We narrowed to 23,600 results for: CHI

1. pcDNA3-Flag-hNF2

   Plasmid

   #107150

   Purpose

   mammalian expression

   Depositor

   Tina Izard

   Article

   Chinthalapudi et al Nat Commun. 2018 Apr 6;9(1):1338. doi: 1

   Insert

   neurofibromin 2 (NF2 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. KA1042_pPB-hCMV*1-Esrrb-IV

   Plasmid

   #124177

   Purpose

   piggyBac-based Dox-inducible expression of Esrrb

   Depositor

   Hans Schöler

   Article

   Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

   Insert

   Esrrb-IRES-Venus (Esrrb Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   tetO-CMVmin

   Available since

   March 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDule2-Tet2.0

   Plasmid

   #85497

   Purpose

   Plasmid for incorporating the non-canonical amino acid Tetrazine 2.0 with the Mj Tet2.0 synthetase and cognate amber suppressing tRNA in Ecoli.

   Depositor

   Ryan Mehl

   Article

   Blizzard et al J Am Chem Soc. 2015 Aug 19;137(32):10044

   Insert

   Tetrazine2.0 tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Y32G L65Q F108S Q109D D158S L162N

   Promoter

   lpp (constitutive)

   Available since

   Jan. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDule-Tet2.0

   Plasmid

   #85496

   Purpose

   Plasmid for incorporating the non-canonical amino acid Tetrazine 2.0 with the Mj Tet2.0 synthetase and cognate amber suppressing tRNA in Ecoli.

   Depositor

   Ryan Mehl

   Article

   Blizzard et al J Am Chem Soc. 2015 Aug 19;137(32):10044

   Insert

   Tetrazine2.0 tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
   

   Use

   Tags

   None

   Expression

   Bacterial

   Mutation

   Y32G L65Q F108S Q109D D158S L162N

   Promoter

   lpp (constitutive)

   Available since

   Jan. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. LAP2 Full I pAcGFP-N1 monomeric GFP (1317)

   Plasmid

   #62044

   Purpose

   mammalian expression of nuclear envelope transmembrane protein

   Depositor

   Eric Schirmer

   Article

   Zuleger et al J Cell Biol. 2011 Apr 4;193(1):109-23. d

   Insert

   LAP2beta (Tmpo Rat)
   

   Use

   Tags

   AcGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV-Myc-FOXA1

   Plasmid

   #219394

   Purpose

   Express FOXA1 in mammalian cells

   Depositor

   Yoichi Furukawa

   Article

   Nakagawa et al Commun Biol. 2024 Apr 29;7(1):510. doi:

   Insert

   FOXA1 (FOXA1 Human)
   

   Use

   Tags

   N terminal Myc tag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMV361_MjTyrRS

   Plasmid

   #193250

   Purpose

   Expresses the M. jannaschii tyrosyl-tRNA synthetase (MjTyrRS)

   Depositor

   Jessica Seeliger, Peter Schultz

   Article

   Touchette et al ACS Infect Dis. 2017 May 12;3(5):336-348

   Insert

   MjTyrRS
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   hsp60

   Available since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pNIC28-Bsa4_ZV-NS5-RdRp

   Plasmid

   #179847

   Purpose

   The RdRp domain (with a 6-histidine tag) from Zika virus nonstructural protein 5 (French Polynesia strain, GenBank accession number ARU07075.1) encompassing residues 270 to 89

   Depositor

   Julien Lescar

   Article

   Gharbi-Ayachi et al J Virol. 2020 Oct 14;94(21). pii: JVI.00

   Insert

   Zika Virus Non-structural protein 5 RdRp domain
   

   Use

   Tags

   6-histine

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1-2xFLAG-2xSTREP (empty vector)

   Plasmid

   #172604

   Purpose

   Empty vector for the cloning of an N-terminal 2xFLAG-2xSTREP-tagged gene of interest, and expression in mammalian cells

   Depositor

   Michele Pagano

   Article

   Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

   Type

   Empty backbone

   Use

   Tags

   2xFLAG-2xSTREP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pB.GFP-KIF1A tail 395-1698

    Plasmid

    #134618

    Purpose

    Mammalian expression of mouse KIF1A 395-1698

    Depositor

    Marvin Bentley

    Article

    Yang et al Traffic. 2019 Aug 28. doi: 10.1111/tra.1

    Insert

    KIF1A (Kif1a Mouse)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    WT with deletion of the 394 N-terminal residues.

    Promoter

    Chicken Beta actin

    Available since

    Jan. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBa.GFP-KIF1C tail 400-1103

    Plasmid

    #134621

    Purpose

    Mammalian expression of human KIF1C 400-1103

    Depositor

    Marvin Bentley

    Article

    Yang et al Traffic. 2019 Aug 28. doi: 10.1111/tra.1

    Insert

    KIF1C (KIF1C Human)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    WT with deletion of the 399 N-terminal residues.

    Promoter

    Chicken Beta actin

    Available since

    Jan. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. NKP38 GFP (1009)

    Plasmid

    #62021

    Purpose

    mammalian expression of nuclear envelope transmembrane protein

    Depositor

    Eric Schirmer

    Article

    Korfali et al Nucleus. 2012 Nov-Dec;3(6):552-64. doi:

    Insert

    AYTL1 (LPCAT2 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

13. pUC-GFP-MCC

    Plasmid

    #133307

    Purpose

    "Burdensome" GFP expressing plasmid carrying microcin-V bacteriocin for plasmid stabilisation

    Depositor

    Chris Barnes

    Article

    Fedorec et al iScience. 2019 Apr 26;14:323-334. doi: 1

    Insert

    microcin-V bacteriocin cassette (cvaC E. coli)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    N112D (please see depositors comments)

    Promoter

    Available since

    Feb. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCb.CAG-HaloTag-GA-ZF-KrabA-KIF5C 378-955

    Plasmid

    #134613

    Purpose

    Mammalian expression of rat KIF5C 378-955

    Depositor

    Marvin Bentley

    Article

    Yang et al Traffic. 2019 Aug 28. doi: 10.1111/tra.1

    Insert

    KIF5C (Kif5c Rat)
    

    Use

    Tags

    Halo

    Expression

    Mammalian

    Mutation

    WT with deletion of the 377 N-terminal residues.

    Promoter

    Chicken Beta actin

    Available since

    April 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTol2CG2-hsp70I:QFGal4-SV40pA

    Plasmid

    #155120

    Purpose

    Tol2 vector for heat-shock inducible expression of QFGal4. Contains cmlc2:mRFP-SV40pA transgenesis marker

    Depositor

    Bret Pearson

    Article

    Burgess et al Dev Biol. 2020 Jul 19. pii: S0012-1606(2

    Insert

    hsp70l:QFGal4 (hsp70l Budding Yeast, Neurospora crassa, Zebrafish)
    

    Use

    Zebrafish expression

    Tags

    Expression

    Mutation

    Promoter

    hsp70l

    Available since

    Sept. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTol2CG2-acta1:QFGal4-SV40pA

    Plasmid

    #155121

    Purpose

    Tol2 vector for skeletal-muscle expression of QFGal4. Contains cmlc2:mRFP-SV40pA transgenesis marker

    Depositor

    Bret Pearson

    Article

    Burgess et al Dev Biol. 2020 Jul 19. pii: S0012-1606(2

    Insert

    acta1:QFGal4
    

    Use

    Zebrafish expression

    Tags

    Expression

    Mutation

    Promoter

    acta1

    Available since

    Sept. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. LentiU6-hASCL1 gRNA_1-MS2-Puro

    Plasmid

    #192670

    Purpose

    Lentiviral expression of sgRNA targeting hASCL1 promoter to activate human ASCL1 transcription

    Depositor

    Jeffrey Miner

    Article

    Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

    Insert

    Human ASCL1 activating gRNA #1 (ASCL1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pOTTC414 - pAAV EF1a hPREP

    Plasmid

    #59967

    Purpose

    An AAV packaging vector that expresses hPREP under control of the EF1a promoter.

    Depositor

    Brandon Harvey

    Article

    Savolainen et al Neurobiol Dis. 2014 Aug;68:1-15. doi: 10

    Insert

    Prolyl oligopeptidase (PREP Human)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Oct. 8, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pRSF-Ivy-his-hLYZ

    Plasmid

    #83920

    Purpose

    Co-expresses a hisx6 tagged copy of the Ivy protein and the wild type human lysozyme protein, both in the cytoplasmic compartment.

    Depositor

    Karl Griswold

    Article

    Lamppa et al J Biotechnol. 2013 Mar 10;164(1):1-8. do

    Inserts

    Ivy
    lysozyme (LYZ Human)
    

    Use

    Tags

    Ivy protein has C-terminal hisx6 tag

    Expression

    Bacterial

    Mutation

    Ivy leader sequence deleted and replaced.

    Promoter

    T7 and t7

    Available since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDule2-3-nitroTyrosine (5B)

    Plasmid

    #85499

    Purpose

    Plasmid for incorporating the non-canonical amino acid 3-nitroTyrosine with the Mj 3NY (5B) synthetase and cognate amber suppressing tRNA in Ecoli.

    Depositor

    Ryan Mehl

    Article

    Cooley et al Biochemistry. 2014 Apr 1;53(12):1916-24.

    Insert

    3-nitroTyrosine tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Y32H H70C D158S I159A L162R

    Promoter

    lpp (constitutive)

    Available since

    Jan. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pET32a-hBTC

    Plasmid

    #199230

    Purpose

    Bacterial production of soluble, active target proteins; Nterm thrombin and enterokinase cleavage sites.

    Depositor

    Nilson Zanchin

    Article

    Ferreira et al Sci Rep. 2022 Mar 23;12(1):5034. doi: 10

    Insert

    Betacellulin (BTC Human)
    

    Use

    Tags

    TrxA-6xHis-S-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pAAV_hSyn1_AchLightR

    Plasmid

    #187463

    Purpose

    Expresses red acetylcholine indicator AchLightR in neuronal cells

    Depositor

    Tommaso Patriarchi

    Article

    Kagiampaki et al Nat Methods. 2023 Sep;20(9):1426-1436. d

    Insert

    AchLightR
    

    Use

    AAV

    Tags

    Igκ leader

    Expression

    Mutation

    Promoter

    human Synapsin-1

    Available since

    July 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pPB-CAG-3xFLAG::Flcn-pgk-hph

    Plasmid

    #48757

    Purpose

    PiggyBac backbone, CAG promoter driving murine 3xFLAG-Flcn fusion, Hygromycin selection

    Depositor

    Austin Smith

    Article

    Betschinger et al Cell. 2013 Apr 11;153(2):335-47. doi: 10

    Insert

    3xFLAG::Flcn (Flcn Mouse)
    

    Use

    Piggybac

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    June 30, 2016

    Availability

    Academic Institutions and Nonprofits only

24. pDONR221-MICU1-mEF

    Plasmid

    #31765

    Depositor

    Vamsi Mootha

    Article

    Perocchi et al Nature. 2010 Sep 16;467(7313):291-6. Epu

    Insert

    MICU1 (MICU1 Human)
    

    Use

    Pdonr gateway

    Tags

    None

    Expression

    Mutation

    Mutated in both EF-hand domains: D231A, E242K, D4…

    Promoter

    Available since

    May 18, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pGEX-E6AP Hect

    Plasmid

    #165102

    Purpose

    bacterial expression of a GST fusion of Human E6AP Hect domain

    Depositor

    Arthur Haas

    Article

    Ronchi et al Methods Mol Biol. 2012;832:197-218. doi:

    Insert

    E6AP Hect (UBE3A Human)
    

    Use

    Tags

    GST from plasmid.

    Expression

    Bacterial

    Mutation

    E6AP Hect domain Amino acids 495-738 (5'QLNP…

    Promoter

    Available since

    March 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCMV-ACBEmax

    Plasmid

    #139106

    Purpose

    Mammalian ACBEmax expression vector (pSI-632)

    Depositor

    Nozomu Yachie

    Article

    Sakata et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

    Insert

    Bi_partite_NLS-TadA*-XTEN_linker-TadA-XTEN_linker-SpCas9(D10A)-SH3_linker-rAPOBEC1-2xUGI-SV40NLS
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available since

    June 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pGIPz-PD-L1 4NQ

    Plasmid

    #121487

    Purpose

    Expression of human PD-L1 4NQ with PD-L1 shRNA

    Depositor

    Mien-Chie Hung

    Article

    Li et al Nat Commun. 2016 Aug 30;7:12632. doi: 10

    Inserts

    PD-L1 4NQ
    hPD-L1 shRNA
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    N35Q, N192Q, N200Q and N219Q

    Promoter

    CMV

    Available since

    Feb. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. USPL1cat-pETM30

    Plasmid

    #85768

    Purpose

    bacterial expression of USPL1 catalytic domain (aa 212-514) as His-GST-TEVsite fusion

    Depositor

    Frauke Melchior

    Article

    Schulz et al EMBO Rep. 2012 Oct;13(10):930-8. doi: 10

    Insert

    USPL1, catalytic domain (aa 212-514) (USPL1 Human)
    

    Use

    Tags

    GST and His

    Expression

    Bacterial

    Mutation

    catalytic domain (aa 212-514)

    Promoter

    T7-lac

    Available since

    March 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. EGFP-hPCM2w/b delta6

    Plasmid

    #47588

    Depositor

    Emanuel Strehler

    Article

    Chicka et al J Biol Chem. 2003 May 16;278(20):18464-7

    Insert

    PMCA2w/b delta6 (ATP2B2 Human)
    

    Use

    Tags

    EGFP-HA-His

    Expression

    Mammalian

    Mutation

    6 C-terminal AA deleted (needed for PDZ binding)

    Promoter

    CMV

    Available since

    Oct. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCb.CAG-HaloTag-GA-ZF-KrabA-KIF5B 376-963

    Plasmid

    #134612

    Purpose

    Mammalian expression of mouse KIF5B 376-963

    Depositor

    Marvin Bentley

    Article

    Yang et al Traffic. 2019 Aug 28. doi: 10.1111/tra.1

    Insert

    KIF5B (Kif5b Mouse)
    

    Use

    Tags

    Halo

    Expression

    Mammalian

    Mutation

    WT with deletion of the 375 N-terminal residues.

    Promoter

    Chicken Beta actin

    Available since

    April 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge