We narrowed to 6,801 results for: CAR

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pET22b-T5-sfGFP*- MmH-MH4R

Plasmid

#196409

Purpose

Express sfGFP with TAG and enzymes for biosynthesizing DOPA

Depositor

Han Xiao

Article

Chen et al J Mol Biol. 2021 Dec 20:167412. doi: 10.

Inserts

sfGFP with TAG at 151 position
Tyrosine Hydroxylase (Th Mouse)
dihydropterine reductase from human (QDPR Human)
pterin-4a-carbinolamine dehydratase from human

Expression

Bacterial

Available Since

Feb. 10, 2023

Availability

Academic Institutions and Nonprofits only

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mcherry-p62 delta PB1 silent

Plasmid

#187985

Purpose

human p62 lacking PB1 domain (=aa1-102) with a silent mutation to get resistance for sip62 #5 (Dharmacon). Internal Reference: SMC552

Depositor

Sascha Martens

Article

Wurzer et al Elife. 2015 Sep 28;4:e08941. doi: 10.755

Insert

p62 (NUP62 Human)

Tags

mCherry

Expression

Mammalian

Mutation

Lacking PB1 domain (aa1-102) with a silent mutati…

Available Since

Oct. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(hYAP1-Y2B)-PGKpuro2AmCherry-W

Plasmid

#163179

Purpose

Lentiviral gRNA plasmid targeting human YAP1 gene, co-expression of mCherry tag

Depositor

Sok Ching Cheong

Article

Chai et al Elife. 2020 Sep 29;9. pii: 57761. doi: 1

Insert

YAP1 (YAP1 Human)

Use

CRISPR and Lentiviral

Tags

mCherry

Expression

Mammalian

Promoter

U6

Available Since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-DTYMK

Plasmid

#100546

Purpose

mammalian expression of deoxythymidylate kinase

Depositor

Yuh-Shan Jou

Article

Yeh et al Oncotarget. 2017 Sep 8;8(44):77734-77751

Insert

deoxythymidylate kinase (DTYMK Human)

Expression

Mammalian

Available Since

Sept. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pGEX Cis1-SH2

Plasmid

#46417

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Cis1 (SOCS1 Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 90-214

Promoter

Tac

Available Since

Sept. 17, 2013

Availability

Academic Institutions and Nonprofits only

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pAAV-iCre-2A-cherry

Plasmid

#182246

Purpose

Expresses iCre linked to mCherry. The promoter is a fragment of the hdc gene promoter which drives pan neuronal expression.

Depositor

William Wisden

Article

Miracca et al J Neurosci. 2022 May 31. pii: JNEUROSCI.

Insert

pan neuronal gene promoter (hdc) fragment driving iCre recombinase and 2A linked to mCherry

Use

AAV and Cre/Lox

Expression

Mammalian

Promoter

fragment of histidine decarboxylase gene promoter…

Available Since

March 4, 2022

Availability

Academic Institutions and Nonprofits only

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p3F-Strep-mBCL10

Plasmid

#33314

Depositor

Vanessa Redecke

Article

Stempin et al J Biol Chem. 2011 Oct 28;286(43):37147-5

Insert

Bcl10 (Bcl10 Mouse)

Tags

3xFLAG and StrepOne

Expression

Mammalian

Promoter

EF1a

Available Since

Dec. 21, 2011

Availability

Academic Institutions and Nonprofits only
pAED2

Plasmid

#234599

Purpose

Fluorescent reporter of the SOS response

Depositor

Tanel Tenson

Article

Kaldalu et al NPJ Antimicrob Resist. 2025 Apr 11;3(1):

Inserts

mScarlet-I
gfp-mut2

Use

Reporter

Expression

Bacterial

Mutation

GFPmut2 was derived from avGFP with the following…

Promoter

Ptet+dnaK P1 and cda

Available Since

April 15, 2025

Availability

Academic Institutions and Nonprofits only

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pMXS-IRES-BLAST flag-CAD T456A S1406A S1859A

Plasmid

#169826

Purpose

Expresses C-terminal flag-tagged CAD with mutation of reported S6K, PKA, and Erk1/2 phosphorylation site

Depositor

Richard Possemato

Article

Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

Insert

CAD (CAD Human)

Use

Retroviral

Tags

Flag tag

Expression

Mammalian

Mutation

T456A S1406A S1859A; TCCC -> AGTC silent mutat…

Available Since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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pFOXA2-T2A-mTagBFP2-PGK-Neo

Plasmid

#223191

Purpose

BFP reporter plasmid targeting the human FOXA2 gene

Depositor

Matthew Birket

Article

Lynch et al EMBO J. 2025 Mar 31. doi: 10.1038/s44318

Insert

FOXA2-T2A-mTagBFP2-PGK-Neo-FOXA2 (FOXA2 Human)

Use

Cre/Lox; Human targeting

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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pRetroX-TRE3G-PLOD2_PGK-GpNLuc

Plasmid

#136457

Purpose

Expresses Tet/Dox-inducible human PLOD2 in mammalian cells.

Depositor

Antonio Amelio

Article

Sato et al Neoplasia. 2021 Jun 6;23(6):594-606. doi

Insert

TRE3G::PLOD2-T2A-Hygromycin (PLOD2 Human)

Use

Luciferase and Retroviral

Expression

Mammalian

Promoter

TRE3G

Available Since

June 7, 2021

Availability

Academic Institutions and Nonprofits only

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pMXs-ms-L-Myc-L351P

Plasmid

#50777

Purpose

retroviral expression of mouse L-Myc L351P mutant

Depositor

Shinya Yamanaka

Article

Nakagawa et al Proc Natl Acad Sci U S A. 2010 Aug 10;10

Insert

L-Myc-L351P (Mycl Mouse)

Use

Retroviral

Expression

Mammalian

Mutation

L351P (does not bind to Max)

Available Since

Feb. 28, 2014

Availability

Academic Institutions and Nonprofits only

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pAFMW-Nbr-D435A/E437A

Plasmid

#40829

Purpose

Expresses Flag, Myc tagged mutant Nbr in Drosophila cells

Depositor

Phillip Zamore

Article

Han et al Curr Biol. 2011 Nov 22;21(22):1878-87. E

Insert

Nbr D435A/E437A (Nbr Fly)

Tags

3xFLAG and 6xMyc

Expression

Insect

Mutation

D435A/E437A, part of the DEDD motif; mutations p…

Promoter

Actin5C

Available Since

Sept. 4, 2013

Availability

Academic Institutions and Nonprofits only
pGEX RASGAP(NC)-SH2

Plasmid

#46433

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Gap/RASGAP(NC) (RASA1 Human)

Tags

GST

Expression

Bacterial

Mutation

contains amino acids 171-448

Promoter

Tac

Available Since

Aug. 12, 2013

Availability

Academic Institutions and Nonprofits only

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pGEX tensin-SH2

Plasmid

#46525

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

tensin (TNS1 Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 1451-1588

Promoter

Tac

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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XE129 beta2AR Fz1-pcDNA3.1

Plasmid

#16795

Depositor

Randall Moon

Article

Liu et al Proc Natl Acad Sci U S A. 1999 Dec 7;96(

Insert

beta 2 AR Fz1 (Fzd1, Adrb2 Rat)

Use

Xenopus expression

Mutation

Chimera of beta 2 adrenergic receptor (extracellu…

Available Since

March 13, 2008

Availability

Academic Institutions and Nonprofits only
pCMV-GABRA1(A322D)-IRES2-EGFP

Plasmid

#170821

Purpose

human GABAA receptors alpha1 subunit carrying A322D mutation and IRES2-EGFP

Depositor

Tingwei Mu

Article

Fu et al PLoS One. 2018 Nov 27;13(11):e0207948. d

Insert

Human GABAA receptor alpha1 subunit carrying A322D mutation (GABRA1 Human)

Tags

IRES2-EGFP

Expression

Mammalian

Mutation

change Alanine 322 to Aspartate

Available Since

Aug. 10, 2021

Availability

Academic Institutions and Nonprofits only

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6xHis-TEV-mCherry-LC3B-Gly (Microtubule-associated proteins 1A/1B light chain 3B)

Plasmid

#169168

Purpose

Conjugatable form of LC3B lacking 5 C-terminal amino acids (MKLSV), with C-terminal Glycine120 exposed for lipidation reaction.

Depositor

Sascha Martens

Article

Zaffagnini et al EMBO J. 2018 Mar 1;37(5). pii: embj.2017

Insert

MAP1LC3B (MAP1LC3B Human)

Tags

6X Histidine Tag, TEV cleavage site, mCherry

Expression

Bacterial

Promoter

T7 lac promoter

Available Since

May 6, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-N-Myc_884-1102 USP7

Plasmid

#131249

Purpose

Mammalian expression of the USP7 UBL4-5 domains with an N-terminal Myc tag

Depositor

Roger Woodgate

Article

Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

Insert

Ubiquitin carboxyl-terminal hydrolase 7 (USP7 Human)

Tags

Myc

Expression

Mammalian

Mutation

Encodes USP7 UBL domains 4-5 (USP7 amino acids 88…

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-hAR-R617M,K618M,K632M,K633M

Plasmid

#115778

Purpose

Expresses human androgen receptor (R617M, K618M, K632M, K633M) in mammalian cells

Depositor

Elizabeth Wilson

Article

Zhou et al J Biol Chem. 1994 May 6;269(18):13115-23

Insert

Androgen Receptor (AR Human)

Expression

Mammalian

Mutation

R617M, K618M ,K632M, K633M, insertion of Q81 and …

Promoter

CMV

Available Since

Oct. 15, 2018

Availability

Academic Institutions and Nonprofits only

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