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Plasmid#170990PurposeFor bacterial expression of bdSUMO tagged muGFP with R9 peptide and NanoLuc complementation peptide variant #86DepositorInsert14x-His-bdSUMO-R9-muGFP-HiBiT
UseLuciferaseTagsExpressionBacterialMutationPromoterT7Available sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGL3-NRASshort
Plasmid#13799DepositorInsertNRAS (NRAS Human)
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 8, 2007AvailabilityAcademic Institutions and Nonprofits only -
pmirGLO-Smad4-1719
Plasmid#49383PurposeInsert is a fragment of the Smad4 3'-UTR that contains miR-17 and miR-19 binding sites. To be used for 3'UTR assay.DepositorInsertSmad family member 4 (SMAD4 Mouse, Human)
UseLuciferaseTagsExpressionMutationPromoterHuman phosphoglycerate kinase (PGK) promoterAvailable sinceOct. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pPRIME-TREX-GFP-FF3
Plasmid#11667Purpose3rd generation lentiviral transfer vector. pPRIME cloning plasmid with a Tet-responsive promoter (TREX) controlling expression of GFP and miR30-based shRNA targeting firefly luciferaseDepositorInsertFF3
UseLentiviralTagsGFPExpressionMammalianMutationPromoterAvailable sinceMarch 1, 2007AvailabilityAcademic Institutions and Nonprofits only -
-962 human cyclin D1 promoter TCF (1) site mutant pGL3Basic
Plasmid#32733DepositorInsertCCND1 promoter (CCND1 Human)
UseLuciferaseTagsExpressionMutation-75 TCF(1) site CTTTGAT deletionPromoter-962 CCND1Available sinceJan. 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
pPKm-118
Plasmid#90491PurposeExpresses pcDNA3 - CMVmin - 5X UAS - pFR LuciferaseDepositorInsertpFR Luciferase Fluc
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-DRD1-NNES-eLOV-TEVcs-Flag-Gal4-V5
Plasmid#125227PurposeExpresses SPARK DRD1 transmembrane component in mammalian cellsDepositorInsertDRD1-NNES-eLOV-TEVcs-Flag-Gal4-V5
UseAAVTagsFlag, V5ExpressionMammalianMutationPromoterCMVAvailable sinceMay 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGL4 mIL-17 1.1kb promoter
Plasmid#20125DepositorInsertmIL-17 (Il17a Mouse)
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceJan. 16, 2009AvailabilityAcademic Institutions and Nonprofits only -
pET28a Gx-NL2
Plasmid#169107PurposeExpresses protein G fused to 2 copies of NanoLuc. The protein G domain contains an amber stop-codon at position 24 for incorporation of the unnatural amino acid para-benzoylphenylalanine.DepositorInsertprotein G - NanoLuc - NanoLuc
UseTagshis-tag and strep-tagExpressionBacterialMutationAla 24 stopPromoterT7Available sinceJune 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTS2311-Tier1-spacer-PPGK-NLuc_TB
Plasmid#169552PurposeTier-1 expression vector encoding PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 preluded by a 5' spacer sequence (spacer-PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA).DepositorInsertPPGK-NanoLuc Luciferase - mTagBFP fusion
UseTagsExpressionMammalianMutationPromoterPPGKAvailable sinceJune 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
-111 hTF m1
Plasmid#15448DepositorInsertTissue factor promoter (F3 Human)
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 27, 2007AvailabilityAcademic Institutions and Nonprofits only -
pCheck2 Dest (R1-R2) #BGV129
Plasmid#48955PurposeDestination dual luciferase reporter plasmid for checking effectiveness of shRNA. Compatible with standard AttL1-AttL2 Gateway Entry vectors.DepositorTypeEmpty backboneUseLuciferase; Gateway cloning vectorTagsExpressionMutationPromoterSV40Available sinceJune 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
-962 human cyclin D1 promoter EtsB site mutant pGL3Basic
Plasmid#32730DepositorInsertCCND1 (CCND1 Human)
UseLuciferaseTagsExpressionMutation-778 EtsB binding site AGGAA deletionPromoter-962 CCND1Available sinceJan. 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
pNB784
Plasmid#60163PurposeFirefly luciferase (Promega) yeast codon-optimized yellow fluorescent protein (yEVenus) fusion driven by SIC1 promoter.DepositorInsertFLuc-yEVenus
UseTagsExpressionYeastMutationFLuc has A4V & S504G (no functional effect)PromoterSIC1prAvailable sinceMarch 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
-962 human cyclin D1 promoter EtsC site mutant pGL3Basic
Plasmid#32731DepositorInsertCCND1 promoter (CCND1 Human)
UseLuciferaseTagsExpressionMutation-424 EtsC site TTCCT deletionPromoter-962 CCND1 promoterAvailable sinceJan. 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
p.UTA.2.0-GL-2
Plasmid#82440PurposeDual Fluorescent endogenous miRNA sensor. Perfect complementary region of an siRNA control targeting firefly luciferase encoded by the pGL2DepositorInsertLuciferase
UseTagsExpressionMutationThe complementary region for GL2 siRNA control wa…PromoterAvailable sinceMarch 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
p6xAP-1_RE
Plasmid#118054PurposeGBPart - Operator/DNA Response element - 6 copies of the AP-1 binding motifDepositorInsert6 copies of the AP-1 binding motif
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceDec. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
-962 human cyclin D1 promoter TCF (1)(2) sites mutant pGL3Basic
Plasmid#32734DepositorInsertCCND1 promoter (CCND1 Human)
UseLuciferaseTagsExpressionMutation-75 TCF(1) and -68 TCF(2) - CTTTGATCTTTGCT deleti…Promoter-962 CCND1Available sinceJan. 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
pGL3-Control-E2F-3'UTR-Mut
Plasmid#21171DepositorInsertE2F 3'UTR Mutant (E2F1 Human)
UseLuciferaseTagsExpressionMammalianMutation2 miR binding sites mutated. Changed from GCACTTT…PromoterAvailable sinceJune 9, 2009AvailabilityAcademic Institutions and Nonprofits only -
Non-targeting CRISPRi plasmid
Plasmid#154891PurposeHuman non-targeting CRISPRi gRNADepositorInsertNontargeting gRNA
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceNov. 18, 2020AvailabilityAcademic Institutions and Nonprofits only