We narrowed to 3,528 results for: MAK;

1. pLenti-DsRed_IRES_MAPT:EGFP

   Plasmid

   #92196

   Purpose

   Lentiviral overexpression vector to make stable bicistronic cell line for Tau screen

   Depositor

   Huda Zoghbi

   Article

   Rousseaux et al Elife. 2016 Oct 25;5. pii: e19809. doi:

   Insert

   DsRed-IRES-MAPT-EGFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSF3-Flag-CBir-FRB_Myc-NBir-FKBP

   Plasmid

   #90003

   Purpose

   Split-BioID plasmid: Expresses N-terminally tagged CBir[257-321]-FRB and N-terminally tagged NBir[2-256]-FKBP

   Depositor

   Julien Béthune

   Article

   Schopp et al Nat Commun. 2017 Jun 6;8:15690. doi: 10.

   Inserts

   NBirA*-linker-FKBP
   CBirA*-linker-FRB
   

   Use

   Retroviral; Flp/frt

   Tags

   FLAG and Myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDONR_P2R-P3_R2-Turbo-NES-mVenus-STOP-L3

   Plasmid

   #127353

   Purpose

   gateway entry vector for making C-terminal TurboID-mVenus fusion with non-nuclear proteins

   Depositor

   Dominique Bergmann

   Article

   Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

   Insert

   TurboID (BirA mutant)
   

   Use

   Gateway entry vector

   Tags

   GS linker, NES, V5, and mVenus

   Expression

   Mutation

   Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

   Promoter

   no promoter

   Available since

   Sept. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSF3-Flag-Cbir-Ago2_Myc-Nbir-Dicer

   Plasmid

   #90013

   Purpose

   Split-BioID plasmid: Expresses N-terminally tagged CBir[257-321]-Ago2 and N-terminally tagged NBir[2-256]-Dicer

   Depositor

   Julien Béthune

   Article

   Schopp et al Nat Commun. 2017 Jun 6;8:15690. doi: 10.

   Inserts

   CBirA*-Ago2
   NBirA*-Dicer
   

   Use

   Retroviral; Flp/frt

   Tags

   FLAG and Myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDONR_P2R-P3_R2-miniTurbo-NES-mVenus-STOP-L3

   Plasmid

   #127355

   Purpose

   gateway entry vector for making C-terminal miniTurbo-mVenus fusion with non-nuclear proteins

   Depositor

   Dominique Bergmann

   Article

   Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

   Insert

   miniTurbo (BirA mutant)
   

   Use

   Gateway entry vector

   Tags

   GS linker, NES, V5, and mVenus

   Expression

   Mutation

   aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

   Promoter

   no promoter

   Available since

   Sept. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMGS46 (ARF16-PB1-HA-P2A-OsTIR1)

   Plasmid

   #126580

   Purpose

   A repair construct to express ARF16-PB1-HA and OsTIR1 under the control of the CMV promoter from the human AAVS1 locus

   Depositor

   Michael Guertin

   Article

   Sathyan et al Genes Dev. 2019 Oct 1;33(19-20):1441-145

   Insert

   OsARF16-PB1 domain
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. TRE-mCardinal-P2A-B19L Flp-in vector

   Plasmid

   #178519

   Purpose

   For making TRE-CB mouse line in Tigre locus

   Depositor

   Ian Wickersham

   Article

   Jin et al Nat Neurosci. 2024 Jan 11. doi: 10.1038/

   Insert

   mCardinal-P2A-B19L
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAWPSG-Po-nCas9-BE- Ptac-sgRNA(mmoX_1)

   Plasmid

   #195741

   Purpose

   Base editing for making a pre-stop codon in mmoX using phenol inducible system

   Depositor

   Seung-Goo Lee

   Article

   Jeong et al Chem Eng J, 2023

   Insert

   Di-Methyl Phenol Regulatory protein / APOBEC1-nCas9(D10A)-UGI / Ptac-RiboJ-sgRNA(mmoX_1)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Po (phoenol-inducible promoter)

   Available since

   March 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV_OHu19986C_myc tag

   Plasmid

   #183174

   Purpose

   used to make AAV vectors for hsTRPA1 tagged with myc

   Depositor

   Sreekanth Chalasani

   Article

   Duque et al Nat Commun. 2022 Feb 9;13(1):600. doi: 1

   Insert

   hsTRPA1 (TRPA1 Human)
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDONR_P2R-P3_R2-miniTurbo-mVenus-STOP-L3

    Plasmid

    #127356

    Purpose

    gateway entry vector for making C-terminal miniTurbo-mVenus fusion with nuclear and non-nuclear proteins

    Depositor

    Dominique Bergmann

    Article

    Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

    Insert

    miniTurbo (BirA mutant)
    

    Use

    Gateway entry vector

    Tags

    GS linker, V5, and mVenus

    Expression

    Mutation

    aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

    Promoter

    no promoter

    Available since

    Sept. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMGS56 (GFP-ARF16-PB1-P2A-OsTIR1)

    Plasmid

    #129668

    Purpose

    A repair construct to express GFP-ARF16-PB1 and OsTIR1 under the control of the CMV promoter from the human AAVS1 locus

    Depositor

    Michael Guertin

    Article

    Sathyan et al Genes Dev. 2019 Oct 1;33(19-20):1441-145

    Insert

    OsARF16-PB1 domain
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pB-CAG-B19L-IRES-mCherry-WPRE-BGHpA

    Plasmid

    #178518

    Purpose

    For making Rabies virus SAD B19 strain L cell line with hyPBase

    Depositor

    Ian Wickersham

    Article

    Jin et al Nat Neurosci. 2024 Jan 11. doi: 10.1038/

    Insert

    B19L-IRES-mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCDNA3-HA-Akt1-K179M

    Plasmid

    #73409

    Purpose

    Expresses kinase-inactive mutant of Akt with HA tag

    Depositor

    Jie Chen

    Article

    Zeng et al Mol Biol Cell. 2014 Dec 1;25(24):3954-61

    Insert

    AKT1 (AKT1 Human)
    

    Use

    Tags

    HA tag

    Expression

    Mammalian

    Mutation

    changed K179M to make Kinase inactive mutant

    Promoter

    CMV

    Available since

    April 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA4/TO/GFP-ZNF598

    Plasmid

    #141191

    Purpose

    Expresses GFP-ZNF598 in mammalian cells, can be used to make inducible cell line

    Depositor

    Simon Bekker-Jensen

    Article

    Tollenaere et al Cell Rep. 2019 Mar 26;26(13):3511-3521.e

    Insert

    ZNF598 (ZNF598 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSF3-Flag-Cbir-Ago2_Myc-Nbir-GFP

    Plasmid

    #90015

    Purpose

    Split-BioID plasmid: Expresses N-terminally tagged CBir[257-321]-Ago2 and N-terminally tagged NBir[2-256]-GFP

    Depositor

    Julien Béthune

    Article

    Schopp et al Nat Commun. 2017 Jun 6;8:15690. doi: 10.

    Inserts

    CBirA*-Ago2
    NBirA*-GFP
    

    Use

    Retroviral; Flp/frt

    Tags

    FLAG and Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. Transposon-for RMCE_Sleeping Beauty_LIR-CAG-Frt-red(katushka)-P2A-Puro-F3-PA-RIR

    Plasmid

    #67275

    Purpose

    For sleeping beauty transposition and establishment of an recombinase mediated cassette exchange docking site. To make RMCE in cell line instead of flp in cell lines

    Depositor

    Jannik Elverløv-Jakobsen

    Article

    Callesen et al PLoS One. 2016 Aug 19;11(8):e0161471. do

    Insert

    puromycin resistant gene
    

    Use

    Tags

    linked to red flouresent protein gene (katushka)

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    July 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. CaMKIV-dCT

    Plasmid

    #126422

    Purpose

    Plasmid expresses constitutively active form of CaMK IV (C-terminal regulatory domains removed making the enzyme constitutively active)

    Depositor

    Douglas Black

    Article

    Xie et al Nature. 2001 Apr 19;410(6831):936-9. doi

    Insert

    Calcium/calmodulin-dependent protein kinase type IV (CAMK4 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSF3-Flag-Cbir-Cdc25C WT_Myc-Nbir-14-3-3e

    Plasmid

    #90010

    Purpose

    Split-BioID plasmid: Expresses N-terminally tagged CBir[257-321]-Cdc25C and N-terminally tagged NBir[2-256]-14-3-3e

    Depositor

    Julien Béthune

    Article

    Schopp et al Nat Commun. 2017 Jun 6;8:15690. doi: 10.

    Inserts

    CBirA*-Cdc25C WT
    NBirA*-14-3-3e
    

    Use

    Retroviral; Flp/frt

    Tags

    FLAG and Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pJC5-4-insulator-BREreporter-insulator

    Plasmid

    #171499

    Purpose

    injections to make Bmp reporter mouse transgenic line

    Depositor

    James Briscoe

    Article

    Ivanovitch et al PLoS Biol. 2021 May 17;19(5):e3001200. d

    Insert

    Insulator BRE-MLP:H2B-Turqoise
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    BRE-MLP

    Available since

    March 23, 2022

    Availability

    Academic Institutions and Nonprofits only

20. pAAV_GW_Dest

    Plasmid

    #86955

    Purpose

    Gateway destination transfer plasmid for making AAV. Contains a DIO cassette for cre-induction. Without cre, insert is backwards in reference to promoter.

    Depositor

    Joseph Dougherty

    Article

    Cammack et al Proc Natl Acad Sci U S A. 2020 May 5;117

    Type

    Empty backbone

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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