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Showing: 3381 - 3400 of 5008 results

  1. pLenti-CMV-PtenG129E-Puro

    Plasmid
    #135678
    Purpose
    Constitutive expression (cMV promoter) of G129E mutant Pten cDNA, Puromycin selection
    Depositor
    Florian Karreth
    Insert
    Pten (Pten Mouse)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    G129E
    Promoter
    Available since
    Jan. 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    135678-plasmid-map-sequence-id-327597

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  2. pLenti-CMV-PtenK13E-Puro

    Plasmid
    #135680
    Purpose
    Constitutive expression (cMV promoter) of K13E mutant Pten cDNA, Puromycin selection
    Depositor
    Florian Karreth
    Insert
    Pten (Pten Mouse)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    K13E
    Promoter
    Available since
    March 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    135680-plasmid-map-sequence-id-327598

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  3. pLenti-CMV-PtenY138L-Puro

    Plasmid
    #135679
    Purpose
    Constitutive expression (cMV promoter) of Y138L mutant Pten cDNA, Puromycin selection
    Depositor
    Florian Karreth
    Insert
    Pten (Pten Mouse)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Y138L
    Promoter
    Available since
    March 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    135679-plasmid-map-sequence-id-327594

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  4. pAc5.1B-DmPABPC1-V5His6_D

    Plasmid
    #146093
    Purpose
    Insect Expression of DmPABPC1
    Depositor
    Elisa Izaurralde
    Insert
    DmPABPC1 (pAbp Fly)
    Use
    Tags
    Expression
    Insect
    Mutation
    one non-silent mutation G26S compared to the sequ…
    Promoter
    Available since
    Feb. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    146093-plasmid-map-sequence-id-362948

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  5. pLenti-TRE-PtenC124S-Blast

    Plasmid
    #135675
    Purpose
    Dox-inducible expression (TRE promoter) of C124S mutant Pten cDNA, Blasticidin selection
    Depositor
    Florian Karreth
    Insert
    Pten (Pten Mouse)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    135675-plasmid-map-sequence-id-327582

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  6. pBlue-hFX-HA(AscI)

    Plasmid
    #15404
    Depositor
    Michael Ristow
    Insert
    human frataxin cDNA (FXN Human)
    Use
    Tags
    AU1 and HA
    Expression
    Bacterial
    Mutation
    700 bp insert can be released by digestion with r…
    Promoter
    Available since
    Aug. 17, 2007
    Availability
    Academic Institutions and Nonprofits only
    15404-plasmid-map-sequence-id-275749

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  7. STING-V1

    Plasmid
    #124262
    Purpose
    C-terminally tagged STING with split Venus Construct for studying localisation of STING in different cellular compartments
    Depositor
    Eric Schirmer
    Insert
    TMEM173 (STING1 Human)
    Use
    Tags
    Venus1
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 10, 2019
    Availability
    Academic Institutions and Nonprofits only
    124262-plasmid-map-sequence-id-241476

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  8. V2-STING

    Plasmid
    #124265
    Purpose
    N-terminally tagged STING with split Venus Construct for studying localisation of STING in different cellular compartments
    Depositor
    Eric Schirmer
    Insert
    TMEM173 (STING1 Human)
    Use
    Tags
    Venus2
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 10, 2019
    Availability
    Academic Institutions and Nonprofits only
    124265-plasmid-map-sequence-id-241431

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  9. STING-V2

    Plasmid
    #124264
    Purpose
    C-terminally tagged STING with split Venus Construct for studying localisation of STING in different cellular compartments
    Depositor
    Eric Schirmer
    Insert
    TMEM173 (STING1 Human)
    Use
    Tags
    Venus2
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 10, 2019
    Availability
    Academic Institutions and Nonprofits only
    124264-plasmid-map-sequence-id-241461

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  10. V1-STING

    Plasmid
    #124263
    Purpose
    N-terminally tagged STING with split Venus Construct for studying localisation of STING in different cellular compartments
    Depositor
    Eric Schirmer
    Insert
    TMEM173 (STING1 Human)
    Use
    Tags
    Venus1
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 10, 2019
    Availability
    Academic Institutions and Nonprofits only
    124263-plasmid-map-sequence-id-241474

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  11. pFRT-TODestGFP_RNAseH1

    Plasmid
    #65784
    Purpose
    expresses GFP-tagged RNAseH1 in mammalian cells
    Depositor
    Thomas Tuschl
    Insert
    RNaseH1 (RNASEH1 Human, Zebrafish)
    Use
    Tags
    GFP and V5
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, 2x Tet
    Available since
    June 18, 2015
    Availability
    Academic Institutions and Nonprofits only
    65784-plasmid-map-sequence-id-111845

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  12. pFRT-TODestRFP_RNAseH1

    Plasmid
    #65785
    Purpose
    expresses RFP-tagged RNAseH1 in mammalian cells
    Depositor
    Thomas Tuschl
    Insert
    RNaseH1 (RNASEH1 Human, Zebrafish)
    Use
    Tags
    RFP and V5
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, 2x Tet
    Available since
    June 23, 2015
    Availability
    Academic Institutions and Nonprofits only
    65785-plasmid-map-sequence-id-354766

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  13. pFRT-DestFLAGHA_RNAseH1

    Plasmid
    #65783
    Purpose
    constitutively expresses FLAG-HA-tagged RNAseH1 in mammalian cells
    Depositor
    Thomas Tuschl
    Insert
    RNaseH1 (RNASEH1 Human, Zebrafish)
    Use
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, 2x Tet
    Available since
    June 18, 2015
    Availability
    Academic Institutions and Nonprofits only
    65783-plasmid-map-sequence-id-151430

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  14. lentiGuide-Puro

    Plasmid
    #52963
    Purpose
    Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and puromycin resistance from EF-1a promoter. 3rd generation lentiviral backbone.
    Depositor
    Feng Zhang
    Article
    Sanjana et al Nat Methods. 2014 Aug;11(8):783-4. doi:
    Has Service
    Cloning Grade DNA
    Inserts
    S. pyogenes sgRNA cassette
    Puromycin resistance
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Ef1-a and hU6
    Available since
    May 8, 2014
    Availability
    Academic Institutions and Nonprofits only
    52963-plasmid-map-sequence-id-331247

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  16. lentiSAMv2

    Plasmid
    #75112
    Purpose
    lenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2, dCas9-VP64, and blast resistance marker. Contains BsmBI sites for insertion of spacer sequences.
    Depositor
    Feng Zhang
    Article
    Joung et al Nat Protoc. 2017 Apr;12(4):828-863. doi:
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6 and EF1A
    Available since
    May 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    75112-plasmid-map-sequence-id-305764

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  18. TLCV2

    Plasmid
    #87360
    Purpose
    LentiCRISPR v2 was modified into an all-in-one dox inducible system. The addition of doxycycline induces Cas9-2A-eGFP. The U6 promoter drives constitutive sgRNA expression.
    Depositor
    Adam Karpf
    Article
    Barger et al Cancers (Basel). 2019 Feb 21;11(2). pii:
    Has Service
    Cloning Grade DNA
    Insert
    Cas9-2A-eGFP
    Use
    CRISPR and Lentiviral; Doxycycline inducible; egf…
    Tags
    Cas9-T2A-eGFP
    Expression
    Mammalian
    Mutation
    Promoter
    Tight TRE promoter
    Available since
    Feb. 24, 2017
    Availability
    Academic Institutions and Nonprofits only
    87360-plasmid-map-sequence-id-473508

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Showing: 3381 - 3400 of 5008 results