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pcDNA3.3_3xFLAG_SUMO_Eu1_HsAGO2(H56A, R68A, R97A, K98A)

Plasmid

#231379

Purpose

Express mutant human AGO2 with N-terminal 3xFLAG-SUMO_Eu1 tag in mammalian cells

Depositor

David Bartel

Publication

Mohamed et al Cell Rep. 2025 Jan 28;44(1):115166. doi:

Insert

Human Argonaute2 (AGO2 Synthetic, Human)

Tags

3xFLAG-SUMO_Eu1

Expression

Mammalian

Mutation

H56A, R68A, R97A, K98A

Promoter

CMV

Available since

Jan. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.3_3xFLAG_SUMO_Eu1_HsAGO2(R97E, K98E)

Plasmid

#231380

Purpose

Express mutant human AGO2 with N-terminal 3xFLAG-SUMO_Eu1 tag in mammalian cells

Depositor

David Bartel

Publication

Mohamed et al Cell Rep. 2025 Jan 28;44(1):115166. doi:

Insert

Human Argonaute2 (AGO2 Synthetic, Human)

Tags

3xFLAG-SUMO_Eu1

Expression

Mammalian

Mutation

R97E, K98E

Promoter

CMV

Available since

Jan. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.3_3xFLAG_SUMO_Eu1_HsAGO2(R710A)

Plasmid

#231381

Purpose

Express mutant human AGO2 with N-terminal 3xFLAG-SUMO_Eu1 tag in mammalian cells

Depositor

David Bartel

Publication

Mohamed et al Cell Rep. 2025 Jan 28;44(1):115166. doi:

Insert

Human Argonaute2 (AGO2 Synthetic, Human)

Tags

3xFLAG-SUMO_Eu1

Expression

Mammalian

Mutation

R710A

Promoter

CMV

Available since

Jan. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.3_3xFLAG_SUMO_Eu1_HsAGO2(H712A)

Plasmid

#231382

Purpose

Express mutant human AGO2 with N-terminal 3xFLAG-SUMO_Eu1 tag in mammalian cells

Depositor

David Bartel

Publication

Mohamed et al Cell Rep. 2025 Jan 28;44(1):115166. doi:

Insert

Human Argonaute2 (AGO2 Synthetic, Human)

Tags

3xFLAG-SUMO_Eu1

Expression

Mammalian

Mutation

H712A

Promoter

CMV

Available since

Jan. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.3_3xFLAG_SUMO_Eu1_HsAGO2(R710A, H712A)

Plasmid

#231383

Purpose

Express mutant human AGO2 with N-terminal 3xFLAG-SUMO_Eu1 tag in mammalian cells

Depositor

David Bartel

Publication

Mohamed et al Cell Rep. 2025 Jan 28;44(1):115166. doi:

Insert

Human Argonaute2 (AGO2 Synthetic, Human)

Tags

3xFLAG-SUMO_Eu1

Expression

Mammalian

Mutation

R710A, H712A

Promoter

CMV

Available since

Jan. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.3_3xFLAG_SUMO_Eu1_HsAGO2(R635A)

Plasmid

#231384

Purpose

Express mutant human AGO2 with N-terminal 3xFLAG-SUMO_Eu1 tag in mammalian cells

Depositor

David Bartel

Publication

Mohamed et al Cell Rep. 2025 Jan 28;44(1):115166. doi:

Insert

Human Argonaute2 (AGO2 Synthetic, Human)

Tags

3xFLAG-SUMO_Eu1

Expression

Mammalian

Mutation

R635A

Promoter

CMV

Available since

Jan. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.3_3xFLAG_SUMO_Eu1_HsAGO2(EI 5xE)

Plasmid

#231385

Purpose

Express mutant human AGO2 with N-terminal 3xFLAG-SUMO_Eu1 tag in mammalian cells

Depositor

David Bartel

Publication

Mohamed et al Cell Rep. 2025 Jan 28;44(1):115166. doi:

Insert

Human Argonaute2 (AGO2 Synthetic, Human)

Tags

3xFLAG-SUMO_Eu1

Expression

Mammalian

Mutation

S824E, S828E, T830E, S831E, S834E

Promoter

CMV

Available since

Jan. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.3_3xFLAG_SUMO_Eu1_HsAGO2(D669A)

Plasmid

#231371

Purpose

Express catalytically dead mutant human AGO2 with N-terminal 3xFLAG-SUMO_Eu1 tag in mammalian cells

Depositor

David Bartel

Publication

Mohamed et al Cell Rep. 2025 Jan 28;44(1):115166. doi:

Insert

Human Argonaute2 (AGO2 Synthetic, Human)

Tags

3xFLAG-SUMO_Eu1

Expression

Mammalian

Mutation

D669A

Promoter

CMV

Available since

Jan. 14, 2025

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCMV_Myc-HalM-P2A-FLAG-YFast-TEV-His-HalB

Plasmid

#225064

Purpose

Expression of HalM-modified HalA2 in human cells

Depositor

Wilfred van der Donk

Publication

Eslami et al ACS Synth Biol. 2024 Jul 19;13(7):2128-2

Insert

HalA2

Tags

Multiple tags due to polycistronic expression

Expression

Mammalian

Mutation

WT

Promoter

CMV

Available since

Oct. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pH6-2xmNeon

Plasmid

#178462

Purpose

A tandem mNeonGreen fluorescent protein (2xmNeon) with an N-terminal 6xHis-tag in pET28a.

Depositor

Siegfried Musser

Publication

Sharma et al Commun Biol. 2022 Sep 19;5(1):988. doi:

Insert

mNeonGreen dimer

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pTatABmNeonC

Plasmid

#178464

Purpose

pTatABC with an mNeonGreen fluorescent protein tag at the C-terminal end of TatB.

Depositor

Siegfried Musser

Publication

Sharma et al Commun Biol. 2022 Sep 19;5(1):988. doi:

Insert

TatA, TatBmNeon, TatC

Tags

mNeonGreen fused to TatB

Expression

Bacterial

Promoter

araC

Available since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pBAD_TatABmCherryC

Plasmid

#178545

Purpose

pTatABC with an mCherry fluorescent protein tag at the end of TatB.

Depositor

Siegfried Musser

Publication

Sharma et al Commun Biol. 2022 Sep 19;5(1):988. doi:

Insert

TatA, TatBmCherry, TatC

Tags

mCherry fused to TatB

Expression

Bacterial

Promoter

araC

Available since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pPB UbC Arrestin-uTEV1-V5; UAS-mCherry

Plasmid

#173122

Purpose

Expresses uTEV1 and reporter receiver construct in DIPG

Depositor

Alice Ting

Publication

Cho et al Elife. 2022 Mar 21;11:e70881. doi: 10.75

Insert

Arrestin-uTEV1-V5; UAS-mCherry

Expression

Mammalian

Promoter

UbC

Available since

Dec. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG CAG CCL20-pre-mGRASP

Plasmid

#173114

Purpose

Expresses CCL20 sender construct in mammalian cells

Depositor

Alice Ting

Publication

Cho et al Elife. 2022 Mar 21;11:e70881. doi: 10.75

Insert

CCL20-pre-mGRASP

Expression

Mammalian

Promoter

CAG

Available since

Dec. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pCDNA3 CMV myc-Arrestin-TEVp

Plasmid

#173115

Purpose

Expresses arrestin receiver construct in mammalian cells

Depositor

Alice Ting

Publication

Cho et al Elife. 2022 Mar 21;11:e70881. doi: 10.75

Insert

myc-Arrestin-TEVp

Expression

Mammalian

Promoter

CMV

Available since

Dec. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pPB UbC Arrestin-TEVp(WT)-V5; UAS-mCherry

Plasmid

#173121

Purpose

Expresses TEVp(WT) and reporter receiver construct in DIPG

Depositor

Alice Ting

Publication

Cho et al Elife. 2022 Mar 21;11:e70881. doi: 10.75

Insert

Arrestin-TEVp(WT)-V5; UAS-mCherry

Expression

Mammalian

Promoter

UbC

Available since

Dec. 21, 2022

Availability

Academic Institutions and Nonprofits only

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NK72

Plasmid

#176608

Purpose

To test macrophage engulfment initiation. Lacks the FcyR chain.

Depositor

Meghan Morrissey

Publication

Kern et al Elife. 2021 Jun 3;10. pii: 68311. doi: 1

Insert

CD86 (CD86 Human)

Use

Lentiviral

Tags

HA, SNAP-tag and mEGFP

Expression

Mammalian

Available since

July 8, 2022

Availability

Academic Institutions and Nonprofits only

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p8130 MSCV GFP Puro C-FlagHA

Plasmid

#184521

Purpose

Expression of GFP

Depositor

Elizabeth White

Publication

Hatterschide et al Elife. 2022 Feb 16;11. pii: 75466. doi:

Insert

GFP

Use

Retroviral

Tags

Flag, HA

Promoter

MSCV LTR

Available since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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p8322 pDONR ATG-YAP1 S94A-Stop

Plasmid

#184526

Purpose

Subcloning YAP1 S94A

Depositor

Elizabeth White

Publication

Hatterschide et al Elife. 2022 Feb 16;11. pii: 75466. doi:

Insert

YAP1 S94A (YAP1 Human)

Use

Unspecified

Mutation

S94A

Available since

June 8, 2022

Availability

Academic Institutions and Nonprofits only

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p8321 pDONR ATG-YAP1 S127A-Stop

Plasmid

#184525

Purpose

Subcloning YAP1 S127A

Depositor

Elizabeth White

Publication

Hatterschide et al Elife. 2022 Feb 16;11. pii: 75466. doi:

Insert

YAP1 S127A (YAP1 Human)

Use

Unspecified

Mutation

S127A

Available since

June 7, 2022

Availability

Academic Institutions and Nonprofits only

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