We narrowed to 14,112 results for: CAN
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Plasmid#108455PurposeThis plamid can be used to recombine the iTol2-Amp-γ-crystallin:RFP into a BAC.DepositorInsertTol2-Amp-y-cryst-mCherry
UseGatewayAvailable SinceSept. 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
STAGR_gRNAScaffold_mU6
Plasmid#102844PurposeCan be used as PCR template for a STAgR reactionDepositorInsertSTAgR Insert gRNAScaffold_mU6
UsePcr template for stagr insertsPromotermU6Available SinceFeb. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCfb12620
Plasmid#219928PurposePrTPI with the overhangs for SapI restriction site can be insert into the base plasmid of TUNEYALIDepositorInsertPromoter TPI
ExpressionYeastAvailable SinceJune 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
SiC-V1
Plasmid#133041PurposeSiC-V1 vector with SpCas9 gene and dTomato reporter. sgRNA targeting a gene of interest can be cloned downstream of U6 promoter.DepositorInsertSpCas9
UseCRISPR and LentiviralTagsdTomatoAvailable SinceNov. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCS2+ GFP-2xESP3I-IRES-mCherry
Plasmid#230996PurposeProtein stability reporter construct for transient expression in mammalian cells. Stability of N-terminal GFP-fusion protein can be assessed by flow cytometry by normalizing to mCherry expression.DepositorTypeEmpty backboneTagsAcGFP1ExpressionMammalianPromoterCMVAvailable SinceJan. 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
iTol2Amp-γ-crystallin:venusGFP
Plasmid#108462PurposeThis plamid can be used to recombine the iTol2-Amp-γ-crystallin:RFP into a BAC.DepositorInsertiTol2Amp crystGFP
Available SinceSept. 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pY2ShHELIX_sgRNA_entry (CJT30)
Plasmid#181781PurposeExpresses Y2-ShHELIX containing a nicking Y2 I-AniI fusion to ShTnsB. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).DepositorInsertY2-nAniI-ShTnsB, ShTnsC, ShTniQ, ShCas12k
ExpressionBacterialMutationY2-nAniI = F13Y, S111Y, K227M, F80K, L232KPromoterLac and J23119Available SinceFeb. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
STAGR_gRNAScaffold_hH1
Plasmid#102841PurposeCan be used as PCR template for a STAgR reactionDepositorInsertSTAgR Insert gRNAScaffold_hH1
UsePcr template for stagr insertsPromoterhH1Available SinceMay 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pT-GEM(2)
Plasmid#62894PurposeTrojan-Gal4 Expression Module in Phase 2. Insert T2A-Gal4 to genomic loci using the Crispr/Cas technology. Can be converted by cassette exchange into other Trojan exonsDepositorTypeEmpty backboneAvailable SinceMay 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
STAgR_Neo
Plasmid#102992PurposeCan be used as backbone for a STAgR reactionDepositorTypeEmpty backboneUseCRISPR; Pcr template for stagr vectorsPromoterU6Available SinceFeb. 6, 2019AvailabilityAcademic Institutions and Nonprofits only