We narrowed to 23,602 results for: CHI

1. EGFP-hPCM2w/b delta6

   Plasmid

   #47588

   Depositor

   Emanuel Strehler

   Article

   Chicka et al J Biol Chem. 2003 May 16;278(20):18464-7

   Insert

   PMCA2w/b delta6 (ATP2B2 Human)
   

   Use

   Tags

   EGFP-HA-His

   Expression

   Mammalian

   Mutation

   6 C-terminal AA deleted (needed for PDZ binding)

   Promoter

   CMV

   Available since

   Oct. 4, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDONR P2R-P3-EGFP

   Plasmid

   #48349

   Purpose

   Contributes the coding sequence for EGFP as the 3’-module during MultiSite Gateway-cloning of chimeric cDNAs encoding three-part fusion proteins.

   Depositor

   Tomas Santalucia, Anna Planas

   Article

   Buj et al BMC Mol Biol. 2013 Aug 20;14(1):18. doi:

   Insert

   EGFP
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   Aminoacid 40 is valine in our sequence, while it …

   Promoter

   none

   Available since

   April 9, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBa.GFP-KIF13B tail 442-1843

   Plasmid

   #134623

   Purpose

   Mammalian expression of mouse KIF13B 442-1843

   Depositor

   Marvin Bentley

   Article

   Yang et al Traffic. 2019 Aug 28. doi: 10.1111/tra.1

   Insert

   KIF13B (Kif13b Mouse)
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   WT with deletion of the 441 N-terminal residues.

   Promoter

   Chicken Beta actin

   Available since

   Oct. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBiFC-HA-hCaspase 2 FL (C303S)-VC155

   Plasmid

   #49257

   Purpose

   Expresses split fluorescence protein to measure caspase-2 dimerization in mammalian cells.

   Depositor

   Douglas Green

   Article

   Bouchier-Hayes et al Mol Cell. 2009 Sep 24;35(6):830-40. doi:

   Insert

   caspase-2 (CASP2 Human)
   

   Use

   Tags

   HA and split Venus C

   Expression

   Mammalian

   Mutation

   C303S

   Promoter

   CMV

   Available since

   March 14, 2014

   Availability

   Academic Institutions and Nonprofits only

5. Sec61 beta pAcGFP-C1 (802)

   Plasmid

   #62008

   Purpose

   mammalian expression of GFP-Sec61 beta

   Depositor

   Eric Schirmer

   Article

   Zuleger et al J Cell Biol. 2011 Apr 4;193(1):109-23. d

   Insert

   Sec61 beta (SEC61B Human)
   

   Use

   Tags

   AcGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 28, 2015

   Availability

   Academic Institutions and Nonprofits only

6. pcDNA-PU.1-ETS

   Plasmid

   #124652

   Purpose

   Chimeric ETS domain

   Depositor

   Gregory Poon

   Article

   Albrecht et al Nucleic Acids Res. 2018 Nov 16;46(20):10

   Insert

   PU.1 ETS domain (residues 167-272) (SPI1, ETS1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. gZiPro

   Plasmid

   #198441

   Purpose

   Activity/inhibition assays, 19F-NMR

   Depositor

   Ute Hellmich, Tanja Schirmeister

   Article

   Maus et al Eur J Med Chem. 2023 Oct 5;258:115573. d

   Insert

   glycine linked Zika virus NS2B-NS3
   

   Use

   Tags

   His6-TEV

   Expression

   Bacterial

   Mutation

   R95*A,

   Promoter

   T7 promoter

   Available since

   March 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-CAG-dLight1.2

   Plasmid

   #111069

   Purpose

   To generate Adeno-Associated viruses for expression of dLight1.1 under CAG promoter

   Depositor

   Lin Tian

   Article

   Patriarchi et al Science. 2018 Jun 29;360(6396). pii: sci

   Has Service

   AAV1

   Insert

   dLight1.2
   

   Use

   AAV

   Tags

   Flag tag

   Expression

   Mutation

   Promoter

   Available since

   July 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDONR P2R-P3-V5-6xHis

   Plasmid

   #48347

   Purpose

   Contributes the coding sequence of a V5-6xHis epitope tags cassette as the 3’-module during MultiSite Gateway-cloning of chimeric cDNAs encoding three-part fusion proteins.

   Depositor

   Tomas Santalucia, Anna Planas

   Article

   Buj et al BMC Mol Biol. 2013 Aug 20;14(1):18. doi:

   Insert

   V5 and 6xHis epitope tags cassette
   

   Use

   Gateway entry clone

   Tags

   Expression

   Mutation

   Contains a stop codon at the end of the epitope t…

   Promoter

   none

   Available since

   April 9, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. USPL1cat-pEYFP_C1

    Plasmid

    #85766

    Purpose

    mammalian expression of USPL1 catalytic domain (aa 212-514) as YFP fusion

    Depositor

    Frauke Melchior

    Article

    Schulz et al EMBO Rep. 2012 Oct;13(10):930-8. doi: 10

    Insert

    USPL1, catalytic domain (aa 212-514) (USPL1 Human)
    

    Use

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    catalytic domain (aa 212-514)

    Promoter

    CMV

    Available since

    Jan. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDESTpK7WG-RPS5Ap-mito

    Plasmid

    #163145

    Purpose

    Plant mitoTALEN vector assembly, Destination vector for plants to express ORFs from entry clone with mitochondrial presequence under the RPS5A promoter

    Depositor

    Shin-ichi Arimura

    Article

    Arimura et al Plant J. 2020 Oct 24. doi: 10.1111/tpj.1

    Insert

    RPS5A promoter and mitochondrial presequence in front of attR1 in pk7WG
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Jan. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pPB-CAG-3xFLAG::Fnip1-pgk-hph

    Plasmid

    #48758

    Purpose

    PiggyBac backbone, CAG promoter driving murine 3xFLAG-Fnip1 fusion, Hygromycin selection

    Depositor

    Austin Smith

    Article

    Betschinger et al Cell. 2013 Apr 11;153(2):335-47. doi: 10

    Insert

    3xFLAG::Fnip1 (Fnip1 Mouse)
    

    Use

    Piggybac

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Sept. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

13. pET-Duet1-6xHis-TEV-LC3BGlydelta5C

    Plasmid

    #190237

    Purpose

    Plasmid for the expression and purification of 6xHis-TEV-LC3BGlydelta5C. Internal reference: SMC893.

    Depositor

    Sascha Martens

    Article

    Fracchiolla et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

    Insert

    MAP1LC3B (MAP1LC3B Human)
    

    Use

    Tags

    6xHis-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAS_4xEcoLeuT(CUA)_EF1_AnapRS

    Plasmid

    #174894

    Purpose

    AnapRS expression for amber suppression in mammalian cells; transient or piggy bac mediated integration

    Depositor

    Simon Elsaesser

    Article

    Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

    Insert

    AnapRS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    L38F, M40G, L41P, Y499V, Y500L, Y527A, H537E, L53…

    Promoter

    EF1

    Available since

    Nov. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBa.GFP-KIF3B tail 391-747

    Plasmid

    #134615

    Purpose

    Mammalian expression of mouse KIF3B 391-747

    Depositor

    Marvin Bentley

    Article

    Yang et al Traffic. 2019 Aug 28. doi: 10.1111/tra.1

    Insert

    KIF3B (Kif5b Mouse)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    WT with deletion of the 390 N-terminal residues.

    Promoter

    Chicken Beta actin

    Available since

    April 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBa.FRB-3myc-GS-KIF3A tail 419-702

    Plasmid

    #134614

    Purpose

    Mammalian expression of human KIF3A 419-702

    Depositor

    Marvin Bentley

    Article

    Yang et al Traffic. 2019 Aug 28. doi: 10.1111/tra.1

    Insert

    KIF3A (KIF3A Human)
    

    Use

    Tags

    FRB, 3myc

    Expression

    Mammalian

    Mutation

    WT with deletion of the 418 N-terminal residues.

    Promoter

    Chicken Beta actin

    Available since

    Jan. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEVA1

    Plasmid

    #58953

    Purpose

    Contains a synthetic DNA sequence, VAR1u, that specifies the Var1 protein. The Cox4 targeting signal is fused to the N-terminus to direct import of Var1. Complements var1 mutations in mtDNA.

    Depositor

    Thomas Fox, Thomas Mason

    Article

    Sanchirico et al EMBO J. 1998 Oct 1;17(19):5796-804.

    Insert

    VAR1u, VAR1 recoded for expression from the nucleus
    

    Use

    Yeast ecoli shuttle vector

    Tags

    preCox4 mitochondrial matrix targeting signal

    Expression

    Yeast

    Mutation

    Synthetic gene in standard genetic code

    Promoter

    ADH1

    Available since

    Aug. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

18. pDONR221-MICU1-sh1-rORF

    Plasmid

    #31764

    Depositor

    Vamsi Mootha

    Article

    Perocchi et al Nature. 2010 Sep 16;467(7313):291-6. Epu

    Insert

    MICU1 (MICU1 Human)
    

    Use

    Pdonr gateway

    Tags

    None

    Expression

    Mutation

    RNAi-resistant ORF (rORF). 8 synonymous changes i…

    Promoter

    None

    Available since

    May 4, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pBa.GFP-KIF3C tail 411-796

    Plasmid

    #134616

    Purpose

    Mammalian expression of mouse KIF3C 411-796

    Depositor

    Marvin Bentley

    Article

    Yang et al Traffic. 2019 Aug 28. doi: 10.1111/tra.1

    Insert

    KIF3C (Kif3c Mouse)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    WT with deletion of the 410 N-terminal residues.

    Promoter

    Chicken Beta actin

    Available since

    Jan. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. PGEX-6P1-hNF2-AR mutant

    Plasmid

    #107149

    Purpose

    bacterial expression

    Depositor

    Tina Izard

    Article

    Chinthalapudi et al Nat Commun. 2018 Apr 6;9(1):1338. doi: 1

    Insert

    neurofibromin 2 (NF2 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    A585W-R588K

    Promoter

    Ptac

    Available since

    April 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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21. pDONR221 NLRP1 A66V

    Plasmid

    #166817

    Purpose

    Gateway pDONR vector encoding NLRP1 with the patient mutation A66V (Zhong et al., 2016) (no stop codon)

    Depositor

    Daniel Bachovchin

    Article

    Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

    Insert

    NLRP1 (NLRP1 Human)
    

    Use

    Gateway shuttling vector

    Tags

    Expression

    Mutation

    A66V

    Promoter

    Available since

    March 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pTRE Tight ArchT-GFP

    Plasmid

    #79627

    Purpose

    Tetracycline-dependent expression of ArchT-EGFP in mammalian cells

    Depositor

    Yasunori Hayashi

    Article

    Sakaguchi et al PLoS One. 2015 Jun 15;10(6):e0130163. do

    Insert

    ArchT
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    ArchT is a more sensitive version of Arch (archae…

    Promoter

    Tet responsive Ptight promoter

    Available since

    July 1, 2016

    Availability

    Academic Institutions and Nonprofits only

23. pET32a-hAREG

    Plasmid

    #199229

    Purpose

    Bacterial production of soluble, active target proteins; Nterm thrombin and enterokinase cleavage sites.

    Depositor

    Nilson Zanchin

    Article

    Ferreira et al Sci Rep. 2022 Mar 23;12(1):5034. doi: 10

    Insert

    Amphiregulin preproprotein (AREG Human)
    

    Use

    Tags

    TrxA-6xHis-S-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. NET37 pEGFP-N2 (607)

    Plasmid

    #61990

    Purpose

    mammalian expression of nuclear envelope transmembrane protein

    Depositor

    Eric Schirmer

    Article

    Zuleger et al J Cell Biol. 2011 Apr 4;193(1):109-23. d

    Insert

    NET37 (KIAA1161 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

25. pENTR E2 HSPter-RPS5Ap-mito

    Plasmid

    #163146

    Purpose

    Plant mitoTALEN vector assembly, Entry clone 2 with attL1 L4; Multisite Gateway entry vector to terminate the E1 ORF and having RPS5A promoter with mitochondrial presequence for the E3 ORF

    Depositor

    Shin-ichi Arimura

    Article

    Arimura et al Plant J. 2020 Oct 24. doi: 10.1111/tpj.1

    Insert

    A terminator of Arabidopsis heat shock protein, RPS5A promoter, and mitochondrial presequence
    

    Use

    Entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pBa.GFP-KIF17 tail 400-1038

    Plasmid

    #134617

    Purpose

    Mammalian expression of mouse KIF1A 400-1038

    Depositor

    Marvin Bentley

    Article

    Yang et al Traffic. 2019 Aug 28. doi: 10.1111/tra.1

    Insert

    KIF17 (Kif17 Mouse)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    WT with deletion of the 399 N-terminal residues.

    Promoter

    Chicken Beta actin

    Available since

    March 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pDONR221 NLRP1 P1214R

    Plasmid

    #166822

    Purpose

    Gateway pDONR vector encoding NLRP1 with the patient mutation P1214R (Grandemange et al., 2017) (no stop codon)

    Depositor

    Daniel Bachovchin

    Article

    Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

    Insert

    NLRP1 (NLRP1 Human)
    

    Use

    Gateway shuttling vector

    Tags

    Expression

    Mutation

    P1214R

    Promoter

    Available since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. LentiU6-mNeurog2 gRNA_1-MS2-Puro

    Plasmid

    #192680

    Purpose

    Lentiviral expression of sgRNA targeting mIL1RN promoter to activate mouse Neurog2 transcription

    Depositor

    Jeffrey Miner

    Article

    Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

    Insert

    Mouse Neurog2 activating gRNA #1 (Neurog2 Mouse)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. NET50 pmRFP-N2 (489)

    Plasmid

    #61983

    Purpose

    mammalian expression of nuclear envelope transmembrane protein

    Depositor

    Eric Schirmer

    Article

    Malik et al Cell Mol Life Sci. 2010 Apr;67(8):1353-6

    Insert

    NET50 (DHRS7 Human)
    

    Use

    Tags

    mRFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

30. pOTTC1618 pAAV SYN1 Nuc-EYFP miR-30 FF3

    Plasmid

    #135564

    Purpose

    An AAV vector expressing miR-30a shRNA vs FF luciferase and a nuclear EYFP reporter

    Depositor

    Christopher Richie

    Article

    Venniro et al Proc Natl Acad Sci U S A. 2020 Apr 7;117

    Inserts

    miR-30 FF3
    Nuc-EYFP
    

    Use

    AAV

    Tags

    3xNLS

    Expression

    Mammalian

    Mutation

    Promoter

    SYN1 and hSYN1

    Available since

    Sept. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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