We narrowed to 38,394 results for: ANT

1. pSF279

   Plasmid

   #167674

   Purpose

   Intermediate vector for five gRNAs using GoldenGate, contains ccdB and attL1-L2 sites for Gateway

   Depositor

   Niko Geldner

   Article

   Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

   Insert

   attL1-ccdB-attL2
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pmScarlet3-Peroxi_C1

   Plasmid

   #189769

   Purpose

   Construct for labelling peroxisomes in mammalian cells with peroxisomal targeted mScarlet3

   Depositor

   Dorus Gadella

   Article

   Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

   Insert

   mScarlet3-Peroxi
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pAB1

   Plasmid

   #62547

   Purpose

   broad host range plasmid for IPTG-inducible expression of eGFP. Designed for imaging Pseudomonas aeruginosa activity in biofilms.

   Depositor

   Michael Franklin

   Article

   Walters MC et al Antimicrob Agents Chemother. 2003 Jan;47

   Insert

   eGFP
   

   Use

   Broad host range plasmid

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pTrc

   Available since

   April 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pmTurquoise2-Peroxi

   Plasmid

   #36203

   Purpose

   In vivo visualization of the peroxisome (can be used for colocalization studies)

   Depositor

   Dorus Gadella

   Article

   Goedhart et al Nat Commun. 2012 Mar 20;3:751. doi: 10.1

   Insert

   Peroxisomal targeting sequence
   

   Use

   Tags

   mTurquoise2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 15, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAMBIA1301-RmMYB108

   Plasmid

   #173180

   Purpose

   Overexpresses Rosa multiflora MYB108 in plants

   Depositor

   Daidi Che

   Article

   Dong et al Front Plant Sci. 2021 Jul 27;12:696919.

   Insert

   RmMYB108
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Oct. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGTQL1221YC

   Plasmid

   #61705

   Purpose

   Gateway compatible BiFC vector (pEarleyGate202-YC)

   Depositor

   Yuhai Cui

   Article

   Lu et al Plant J. 2010 Jan;61(2):259-70. doi: 10.

   Type

   Empty backbone

   Use

   Bifc assay

   Tags

   YFP-C and FLAG

   Expression

   Plant

   Mutation

   Promoter

   35S

   Available since

   May 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. OaAEP1_C247A

   Plasmid

   #200307

   Purpose

   Bacterial expression plasmids for production of C247A mutant of asparaginyl endopeptidase 1 from O. affinis (OaAEP1_C247A)

   Depositor

   Artur Krężel

   Article

   Antonenko et al Bioconjug Chem. 2023 Mar 15;34(4):719-27

   Insert

   Asparaginyl endopeptidase from Oldenlandia affinis
   

   Use

   Tags

   Smt3

   Expression

   Bacterial

   Mutation

   Cysteine 247 to Alanine

   Promoter

   T7

   Available since

   June 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. CRISPR_HLA_class_I_1

   Plasmid

   #164986

   Purpose

   Expression of gRNA targeting multiple HLA-A, HLA-B, and HLA-C genes

   Depositor

   Matthew Meyerson

   Article

   Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

   Insert

   gRNA against HLA class I
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pDGE332

   Plasmid

   #153241

   Purpose

   M1 shuttle vector containing the Arabidopsis thaliana U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

   Depositor

   Johannes Stuttmann

   Article

   Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pDGE333

    Plasmid

    #153242

    Purpose

    M2 shuttle vector containing the Arabidopsis thaliana U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

    Depositor

    Johannes Stuttmann

    Article

    Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pDGE335

    Plasmid

    #153244

    Purpose

    M3 shuttle vector containing the Arabidopsis thaliana U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

    Depositor

    Johannes Stuttmann

    Article

    Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. 3XHA-PP2A-Ba

    Plasmid

    #195229

    Purpose

    Retroviral vector containing HA tagged PP2A

    Depositor

    David Bryant, Keith Mostov

    Article

    Bryant et al Dev Cell. 2014 Oct 27;31(2):171-87. doi:

    Insert

    PPP2R2A (PPP2R2A Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. LV

    Plasmid

    #202004

    Purpose

    Empty lenti backbone

    Depositor

    Nikhil Joshi

    Article

    Cui et al Cell. 2021 Dec 9;184(25):6101-6118.e13.

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. PrimeRoot.v2C-FLP

    Plasmid

    #201740

    Purpose

    For DNA insertion test in protoplast

    Depositor

    Caixia Gao

    Article

    Sun et al Nat Biotechnol. 2023 Apr 24. doi: 10.103

    Insert

    ePPE,FLP
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. μ2-HA-WT

    Plasmid

    #32752

    Depositor

    Alexander Sorkin

    Article

    Nesterov et al EMBO J. 1999 May 4;18(9):2489-99.

    Insert

    μ2 subunit of clathrin adaptor complex AP-2 (Ap2m1 Rat)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 17, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pDe-CAS9

    Plasmid

    #61433

    Purpose

    Binary expression vector with A.th. codon-optimized Cas9 and Gateway destination sequence

    Depositor

    Holger Puchta

    Article

    Fauser et al Plant J. 2014 Jul;79(2):348-59. doi: 10.

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    PcUBI4-2

    Available since

    Sept. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pDGE655

    Plasmid

    #153253

    Purpose

    M1 shuttle vector containing the Solanum lycopersicum U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

    Depositor

    Johannes Stuttmann

    Article

    Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. mTurquoise2-C1

    Plasmid

    #54842

    Purpose

    Localization: C1 Cloning Vector, Excitation: 434, Emission: 474

    Depositor

    Michael Davidson, Dorus Gadella

    Article

    Goedhart et al Nat Commun. 2012 Mar 20;3:751. doi: 10.1

    Type

    Empty backbone

    Use

    Tags

    mTurquoise2

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 12, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. Cas9 Null

    Plasmid

    #198727

    Purpose

    Expresses Cas9 without a mobility signal and the mRNA is not graft mobile. Induced by estradiol.

    Depositor

    Friedrich Kragler

    Article

    Yang et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    Cas9
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    pG10-90

    Available since

    May 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. p32Fn

    Plasmid

    #158153

    Purpose

    Construct conventional Cpf1 vectors that uses U3p and UBI10p to express single crRNA (or PTC/CA) and LbCpf1, respectively. For plant genome editing.

    Depositor

    Kabin Xie

    Article

    Ding et al Mol Plant. 2018 Apr 2;11(4):542-552. doi

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Rice codon optimized

    Promoter

    Available since

    Jan. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pRK1043

    Plasmid

    #8835

    Depositor

    David Waugh

    Article

    Kapust et al Protein Eng. 2001 Dec . 14(12):993-1000.

    Insert

    tobacco etch virus protease, catalytic domain
    

    Use

    Tags

    MBP and polyarginine

    Expression

    Bacterial

    Mutation

    Autolysis-resistant S219V mutant

    Promoter

    Available since

    Feb. 14, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pRK5-HA-SZT2

    Plasmid

    #87035

    Purpose

    mammalian expression of SZT2

    Depositor

    David Sabatini

    Article

    Wolfson et al Nature. 2017 Feb 15. doi: 10.1038/nature

    Insert

    SZT2 (SZT2 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pP35S (GB0030)

    Plasmid

    #68163

    Purpose

    Provides the CaMV 35S Promoter as a level 0 GoldenBraid part

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    CaMV 35S promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available since

    Oct. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pJAT11

    Plasmid

    #204290

    Purpose

    For inserting a phiC31 attP site. HDR integrant is marked with removable ie1-DsRed cassette, which is expressed in abdomen and mouthparts.

    Depositor

    David Stern

    Article

    Stern et al bioRxiv 2023.06.17.545412

    Insert

    ie1-DsRed
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    ie1

    Available since

    Sept. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pmTurquoise2-NES

    Plasmid

    #36206

    Purpose

    In vivo visualization of the cytoplasm, but not the nucleus (can be used for colocalization studies)

    Depositor

    Dorus Gadella

    Article

    Goedhart et al Nat Commun. 2012 Mar 20;3:751. doi: 10.1

    Insert

    NES
    

    Use

    Tags

    mTurquoise2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 15, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pLentiGuide_mCherry_sgEPAS1#1

    Plasmid

    #183122

    Purpose

    pLentiGuide modified to express mCherry and an sgRNA targeting EPAS1

    Depositor

    William Kaelin

    Article

    Stransky et al Proc Natl Acad Sci U S A. 2022 Apr 5;119

    Insert

    sgEPAS1-1
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Aug. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pAC-ZEAX

    Plasmid

    #53274

    Purpose

    Contains crtE, crtB, crtI, crtY and crtZ genes of Erwinia herbicola (Pantoea agglomerans) Eho10, and thereby produces zeaxanthin in E. coli

    Depositor

    Francis X Cunningham Jr

    Article

    Sun et al J Biol Chem. 1996 Oct 4;271(40):24349-52

    Insert

    crtE, crtY, crtI, crtB, crtZ
    

    Use

    Low copy number bacterial cloning vector

    Tags

    Expression

    Mutation

    Deleted idi (ORF6) and crtX genes

    Promoter

    endogenous promoters

    Available since

    Jan. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pRU325

    Plasmid

    #167676

    Purpose

    Intermediate vector for eight gRNAs using GoldenGate, contains ccdB and attL1-L2 sites for Gateway

    Depositor

    Niko Geldner

    Article

    Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

    Insert

    attL1-ccdB-attL2
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pLentiCRISPR_Puro_sgAAVS1

    Plasmid

    #187818

    Purpose

    pLentiCRISPR that is selectable with puromycin with an sgRNA targeting AAVS1

    Depositor

    William Kaelin

    Article

    Stransky et al Proc Natl Acad Sci U S A. 2022 Apr 5;119

    Insert

    sgAAVS1
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Aug. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pXEE401BG

    Plasmid

    #91717

    Purpose

    CRISPR/Cas9-mediated genome editing in Arabidopsis, Bar and glyphosate-resistance

    Depositor

    Qi-Jun Chen

    Article

    Wang et al Genome Biol. 2015 Jul 21;16:144. doi: 10

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    July 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pCyterm-mScarlet3_N1

    Plasmid

    #189778

    Purpose

    Construct for testing oligimerization of mScarlet3 in mammalian cells by OSER assay

    Depositor

    Dorus Gadella

    Article

    Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

    Insert

    Cyterm-mScarlet3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pUPD2

    Plasmid

    #68161

    Purpose

    Universal domesticator plasmid for level 0 parts

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    empty vector
    

    Use

    Synthetic Biology; Domestication of dna parts for…

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. HLA_DRB1_07_01

    Plasmid

    #165014

    Purpose

    Expression of HLA-DRB1*07:01 cDNA

    Depositor

    Matthew Meyerson

    Article

    Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

    Insert

    HLA-DRB1*07:01 (HLA-DRB1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pRK5-FLAG-SZT2

    Plasmid

    #87034

    Purpose

    mammalian expression of SZT2

    Depositor

    David Sabatini

    Article

    Wolfson et al Nature. 2017 Feb 15. doi: 10.1038/nature

    Insert

    SZT2 (SZT2 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pH-A3A-PBE

    Plasmid

    #119774

    Purpose

    Conversion of C to T in rice plants transformation

    Depositor

    Caixia Gao

    Article

    Zong et al Nat Biotechnol. 2018 Oct 1. pii: nbt.426

    Insert

    APOBEC3A-nCas9-NLS-UGI-NLS
    

    Use

    Tags

    NLS (flanking UGI)

    Expression

    Plant

    Mutation

    nCas9 has a mutation D10A in wild type SpCas9

    Promoter

    maize Ubiquitin-1

    Available since

    Sept. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pMP71-Flt3L

    Plasmid

    #174600

    Purpose

    Secretes the extracellular domain of murine Flt3L

    Depositor

    Stanley Riddell

    Article

    Veatch et al J Clin Invest. 2021 Aug 16;131(16):e1441

    Insert

    secretes extracellular domain of mouse Flt3-L
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pCAS9-TPC

    Plasmid

    #61478

    Purpose

    Binary expression vector with A.th. codon-optimized Cas9 for conventional cloning

    Depositor

    Holger Puchta

    Article

    Fauser et al Plant J. 2014 Jul;79(2):348-59. doi: 10.

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    PcUbi4-2

    Available since

    Sept. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pTnos (GB0037)

    Plasmid

    #68188

    Purpose

    Provides the Agrobacterium tumefaciens Nopaline synthase terminator as a level 0 GoldenBraid part

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    Nos terminator
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available since

    Oct. 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pTC223

    Plasmid

    #70019

    Purpose

    Cas9/sgRNA targeting ANT1 locus, donor molecule with 5' homology arm-Pnos:NptII-35S:ANT13' homology arm as a Gemini Viral Replicon based on Bean Yellow Dwarf Virus; sgRNA= 7

    Depositor

    Daniel Voytas

    Article

    Cermak et al Genome Biol. 2015 Nov 6;16(1):232. doi:

    Insert

    Nuclease (Cas9/sgRNA) + Donor + GVR
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Dec. 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pDGE1108

    Plasmid

    #202772

    Purpose

    CRISPR-KO in Arabidopsis thaliana

    Depositor

    Johannes Stuttmann

    Article

    Ordon et al Plant Methods. 2023 Mar 28;19(1):30. doi

    Inserts

    FAST marker
    pRPS5a:zCas9_ttriple
    ccdB cassette (BsaI-excisable)
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Aug. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge