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pBa.Halo-KIF3A DD 590-702

Plasmid

#193928

Purpose

Mammalian expression of Halo-tagged Kif 3A DD 590-702

Depositor

Marvin Bentley

Article

Garbouchian et al Mol Biol Cell. 2022 Oct 6:mbcE22080336.

Insert

Kif 3A (KIF3A Human)

Tags

Halo

Expression

Mammalian

Promoter

Chicken Beta actin

Available Since

Dec. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pBa.GFP-KIF3A CC 419-589

Plasmid

#193925

Purpose

Mammalian expression of GFP-tagged Kif 3A CC 419-589

Depositor

Marvin Bentley

Article

Garbouchian et al Mol Biol Cell. 2022 Oct 6:mbcE22080336.

Insert

Kif 3A (KIF3A Human)

Tags

GFP

Expression

Mammalian

Promoter

Chicken Beta actin

Available Since

Dec. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pSN526

Plasmid

#184827

Purpose

Expresses human KIF5A(∆exon27) in insect cells

Depositor

Kyoko Chiba

Article

Nakano et al Genes Cells. 2022 Apr 17. doi: 10.1111/g

Insert

KIF5A (∆exon27) (KIF5A Human)

Tags

mScarlet-2xStrepII

Expression

Insect

Mutation

∆exon27 form of human KIF5A

Promoter

polyhedrin

Available Since

May 25, 2022

Availability

Academic Institutions and Nonprofits only

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pJet-gR-X4ex2-DTS

Plasmid

#177154

Purpose

Donor plasmid for knock-in of MT-C34 peptide into exon-2 of CXCR4

Depositor

Dmitriy Mazurov

Article

Maslennikova et al mBio. 2022 Jan 25:e0358921. doi: 10.1128

Insert

MT-C34-pA with CXCR4 arms of homology

Use

Pcr cloning

Promoter

none

Available Since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pLENTI CMV GFP-TREX1 P290L BLAST

Plasmid

#164239

Purpose

lentiviral plasmid for GFP-TREX1 P290L

Depositor

John Maciejowski

Article

Mohr et al Mol Cell. 2021 Jan 12. pii: S1097-2765(2

Insert

TREX1 (TREX1 Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

P290L point mutation

Promoter

CMV

Available Since

Nov. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pLENTI CMV GFP-TREX1 Y305C BLAST

Plasmid

#164240

Purpose

lentiviral plasmid for GFP-TREX1 Y305C

Depositor

John Maciejowski

Article

Mohr et al Mol Cell. 2021 Jan 12. pii: S1097-2765(2

Insert

TREX1 (TREX1 Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

Y305C point mutation

Promoter

CMV

Available Since

Nov. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pLENTI CMV GFP-TREX1 D272fs BLAST

Plasmid

#164237

Purpose

lentiviral plasmid for GFP-TREX1 D272fs

Depositor

John Maciejowski

Article

Mohr et al Mol Cell. 2021 Jan 12. pii: S1097-2765(2

Insert

TREX1 (TREX1 Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

D272 frame shift mutation

Promoter

CMV

Available Since

Nov. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pLENTI CMV GFP-TREX1 L287fs BLAST

Plasmid

#164238

Purpose

lentiviral plasmid for GFP-TREX1 L287fs

Depositor

John Maciejowski

Article

Mohr et al Mol Cell. 2021 Jan 12. pii: S1097-2765(2

Insert

TREX1 (TREX1 Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

L287 frame shift mutation

Promoter

CMV

Available Since

Nov. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-TREtight-H2b-emiRFP670-P2A-TEVP

Plasmid

#174379

Purpose

AAV virus for expressing TEVP and H2b-emiRFP670

Depositor

Ian Wickersham

Article

Jin et al Proc Natl Acad Sci U S A. 2023 Feb 14;12

Insert

TEVP

Use

AAV

Available Since

Sept. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pRV∆G-N*PEST-4Cre

Plasmid

#174381

Purpose

Rabies vector for expressing Cre recombinase and N gene with a stop codon before PEST domain

Depositor

Ian Wickersham

Article

Jin et al Proc Natl Acad Sci U S A. 2023 Feb 14;12

Insert

Cre recombinase

Expression

Mammalian

Available Since

Sept. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pLENTI CMV GFP-TREX1 309* BLAST

Plasmid

#164242

Purpose

lentiviral plasmid for GFP-TREX1 309*

Depositor

John Maciejowski

Article

Mohr et al Mol Cell. 2021 Jan 12. pii: S1097-2765(2

Insert

TREX1 (TREX1 Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

deletion of amino acids 310-314

Promoter

CMV

Available Since

March 31, 2021

Availability

Academic Institutions and Nonprofits only

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pLENTI CMV GFP-TREX1 G306A BLAST

Plasmid

#164241

Purpose

lentiviral plasmid for GFP-TREX1 G306A

Depositor

John Maciejowski

Article

Mohr et al Mol Cell. 2021 Jan 12. pii: S1097-2765(2

Insert

TREX1 (TREX1 Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

G306A point mutation

Promoter

CMV

Available Since

March 31, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti-FNLSHF1-P2A-Puro

Plasmid

#136901

Purpose

Lentivirus for constitutive expression of FNLSHF1 in mammalian cells (codon optimized)

Depositor

Lukas Dow

Article

Katti et al Nucleic Acids Res. 2020 Feb 29. pii: 576

Insert

FNLSHF1

Use

Lentiviral

Tags

FLAG-NLS and NLS

Mutation

R661A,Q695A,Q926A

Promoter

EF1s

Available Since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pSiren_EGFP-shHmga2#2

Plasmid

#122290

Purpose

Knockdown for mouse Hmga2

Depositor

Yukiko Gotoh

Article

Kishi et al Nat Neurosci. 2012 Aug;15(8):1127-33. do

Insert

Knockdown shRNA for mouse Hmga2 (Hmga2 Mouse)

Use

Retroviral

Expression

Mammalian

Available Since

March 22, 2019

Availability

Academic Institutions and Nonprofits only

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pBW106

Plasmid

#111138

Purpose

For the expression of rat AANAT with Met-Ser-Ile N-terminus in S. cerevisiae

Depositor

Alexander Varshavsky

Article

Wadas et al J Biol Chem. 2016 Aug 12;291(33):17178-9

Insert

MSI-ratAANAT-3FLAG (Leu2 deletion) (Aanat Rat)

Tags

3x Flag

Expression

Yeast

Mutation

Leu2 deletion

Promoter

Gal1

Available Since

June 4, 2018

Availability

Academic Institutions and Nonprofits only

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pSC24

Plasmid

#104798

Purpose

CRISPR/Cas9 2xplex gRNA targeting Medtr2g101120, Medtr2g101130 (Acre2). Also expresses Cas9 from rolD promoter from AtUBQ10 promoter

Depositor

Nevin Young

Article

Curtin et al Plant Physiol. 2017 Feb;173(2):921-931.

Insert

Medtr2g101120, Medtr2g101130

Use

CRISPR

Expression

Plant

Available Since

Jan. 31, 2018

Availability

Academic Institutions and Nonprofits only

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pSC22

Plasmid

#104796

Purpose

CRISPR/Cas9 2xplex gRNA targeting Medtr2g101120, Medtr2g101130 (Acre1). Also expresses Cas9 from Gmubi promoter from AtUBQ10 promoter

Depositor

Nevin Young

Article

Curtin et al Plant Physiol. 2017 Feb;173(2):921-931.

Insert

Medtr2g101120, Medtr2g101130

Use

CRISPR

Expression

Plant

Available Since

Jan. 31, 2018

Availability

Academic Institutions and Nonprofits only

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pMDC84-ScSEIPIN-Ndelta

Plasmid

#96985

Purpose

Express yeast SEIPIN gene in plants.

Depositor

Kent Chapman

Article

Cai et al Plant Cell. 2015 Sep;27(9):2616-36. doi:

Insert

ScSEIPIN (SEI1 Budding Yeast)

Tags

6xHis and GFP

Expression

Plant

Mutation

N-terminal 39 nucleotides were deleted from yeast…

Promoter

35S

Available Since

Aug. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pILGFP88

Plasmid

#83544

Purpose

Used to evaluate the expression output of RPL15A promoter with yEGFP used as the reporter gene.

Depositor

Claudia Vickers

Article

Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

Insert

RPL15A promoter-yEGFP

Expression

Yeast

Promoter

RPL15A

Available Since

Dec. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pILGFP77

Plasmid

#83538

Purpose

Used to evaluate the expression output of RPL8B promoter with yEGFP used as the reporter gene.

Depositor

Claudia Vickers

Article

Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

Insert

RPL8B promoter-yEGFP

Expression

Yeast

Promoter

RPL8B

Available Since

Dec. 15, 2016

Availability

Academic Institutions and Nonprofits only

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