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pLY232

Plasmid

#184149

Purpose

short tracrRNA generator with WT anti-repeat region

Depositor

Baojun Wang

Article

Liu et al Nat Commun. 2022 Apr 11;13(1):1937. doi:

Insert

s-tracrRNA-WT

Use

Synthetic Biology

Expression

Bacterial

Promoter

araBAD promoter

Available Since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pLY237

Plasmid

#184150

Purpose

Reprogrammed short tracrRNA generator for hijacking RFP mRNA as CRISPR RNA (target site 1)

Depositor

Baojun Wang

Article

Liu et al Nat Commun. 2022 Apr 11;13(1):1937. doi:

Insert

s-tracrRNA-R1

Use

Synthetic Biology

Expression

Bacterial

Promoter

araBAD promoter

Available Since

Oct. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pLY251

Plasmid

#184153

Purpose

Reprogrammed short tracrRNA for hijacking mRNA of arsenic responsive gene cluster (site 2)

Depositor

Baojun Wang

Article

Liu et al Nat Commun. 2022 Apr 11;13(1):1937. doi:

Insert

s-tracrRNA-Ar2

Use

Synthetic Biology

Expression

Bacterial

Promoter

araBAD promoter

Available Since

Oct. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pGL2208

Plasmid

#137049

Purpose

Drug Resistance pDONR221BSD

Depositor

Jeremy Mottram

Article

Duncan et al Methods Mol Biol. 2019;1971:211-224. doi

Insert

blasticidin-S deaminase

Use

Leishmania expression gateway vector

Available Since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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pBI-p53 R175H/EGFP

Plasmid

#16544

Depositor

Bert Vogelstein

Article

Yu et al Proc Natl Acad Sci U S A. 1999 Dec 7. 96

Insert

p53 (TP53 Human)

Expression

Mammalian

Mutation

R175H

Available Since

June 20, 2008

Availability

Academic Institutions and Nonprofits only

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MMTV-SV40-Bssk

Plasmid

#1824

Depositor

Philip Leder

Article

Ornitz et al Proc Natl Acad Sci U S A 1991 Feb 1;88(3

Type

Empty backbone

Expression

Mammalian

Available Since

July 18, 2006

Availability

Academic Institutions and Nonprofits only

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pAAV.hSynapsin.SF-iGluSnFR.S72A

Plasmid

#106176

Purpose

Weak affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

Depositor

Loren Looger

Article

Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

Has Service

AAV1

Insert

SF-iGluSnFR.S72A

Use

AAV

Mutation

GltI: S72A

Promoter

hSynapsin

Available Since

June 19, 2018

Availability

Academic Institutions and Nonprofits only

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pET28a SpyCatcher-SnoopCatcher

Plasmid

#72324

Purpose

Bacterial expression of SpyCatcher linked to SnoopCatcher.

Depositor

Mark Howarth

Article

Veggiani et al Proc Natl Acad Sci U S A. 2016 Jan 19. p

Insert

pET28a SpyCatcher-SnoopCatcher

Tags

N-terminal His6 tag

Expression

Bacterial

Mutation

35 amino acids at the N-term of SpyCatcher have b…

Available Since

Feb. 12, 2016

Availability

Academic Institutions and Nonprofits only

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pJFRC28-10XUAS-IVS-GFP-p10

Plasmid

#36431

Depositor

Gerald Rubin

Article

Pfeiffer et al Proc Natl Acad Sci U S A. 2012 Apr 9.

Insert

GFP

Expression

Insect

Available Since

May 3, 2012

Availability

Academic Institutions and Nonprofits only

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Ngn2

Plasmid

#34999

Depositor

Malin Parmar

Article

Pfisterer et al Proc Natl Acad Sci U S A. 2011 Jun 21;10

Insert

Neurogenin-2 (Neurog2 Mouse)

Use

Lentiviral

Expression

Mammalian

Available Since

March 6, 2012

Availability

Academic Institutions and Nonprofits only

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pCMV5 HA-TBRII (delta Cyt)

Plasmid

#14051

Depositor

Joan Massague

Article

Siegel et al Proc Natl Acad Sci U S A. 2003 Jul 8. 10

Insert

TGF beta rec II (delta cyt) (TGFBR2 Human)

Tags

HA (PYDVPDYA)

Expression

Mammalian

Mutation

Dominant negative mutant. A stop codon was introd…

Available Since

May 10, 2007

Availability

Academic Institutions and Nonprofits only

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scFLARE2

Plasmid

#158700

Purpose

Neuronal expression of scFLARE2 component

Depositor

Alice Ting

Article

Sanchez et al Proc Natl Acad Sci U S A. 2020 Dec 15. p

Insert

scFLARE2

Use

AAV

Tags

HA, Flag, VP16

Expression

Mammalian

Mutation

Truncated form of TEV protease (220-242 amino aci…

Promoter

Syn

Available Since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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mRFP1-C1

Plasmid

#54764

Purpose

Localization: C1 Cloning Vector, Excitation: 584, Emission: 607

Depositor

Robert Campbell, Michael Davidson, Roger Tsien

Article

Campbell et al Proc Natl Acad Sci U S A. 2002 Jun 11;99

Type

Empty backbone

Tags

mRFP1

Expression

Mammalian

Available Since

June 11, 2014

Availability

Academic Institutions and Nonprofits only

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pLD-puro-Cc-SARS-CoV-2_Spike_Alpha-VA

Plasmid

#191217

Purpose

Lentiviral expression of SARS-CoV-2 Spike_Alpha in mammalian cells

Depositor

Mohan Babu

Article

Broderick et al Nat Commun. 2025 Jul 1;16(1):5784. doi:

Insert

SPIKE_SARS2_Alpha (S SARS CoV-2)

Use

Lentiviral

Tags

VA tag

Expression

Mammalian

Mutation

Del 69-70 Del 144 N501Y A570D D614G P681H T716I S…

Promoter

CMV

Available Since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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pLD-puro-Cc-SARS-CoV-2_Spike_Delta-VA

Plasmid

#191219

Purpose

Lentiviral expression of SARS-CoV-2 Spike_Delta in mammalian cells

Depositor

Mohan Babu

Article

Broderick et al Nat Commun. 2025 Jul 1;16(1):5784. doi:

Insert

SPIKE_SARS2_Delta (S SARS CoV-2)

Use

Lentiviral

Tags

VA tag

Expression

Mammalian

Mutation

T19R 156del 157del R158G L452R T478K D614G P681R …

Promoter

CMV

Available Since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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pLD-puro-Cc-SARS-CoV-2_Spike_Lambda-VA

Plasmid

#191220

Purpose

Lentiviral expression of SARS-CoV-2 Spike_Lambda in mammalian cells

Depositor

Mohan Babu

Article

Broderick et al Nat Commun. 2025 Jul 1;16(1):5784. doi:

Insert

SPIKE_SARS2_Lambda (S SARS CoV-2)

Use

Lentiviral

Tags

VA tag

Expression

Mammalian

Mutation

G75V T76I Del 246-252 L452Q F490S D614G T859N

Promoter

CMV

Available Since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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pCS2-UTX-F-MT2

Plasmid

#40619

Purpose

Expression of enzyme-dead UTX in mammalian cells

Depositor

Kai Ge

Article

Wang et al Proc Natl Acad Sci U S A. 2012 Sep 4.

Insert

UTX (KDM6A Human)

Tags

6xMyc and Flag

Expression

Mammalian

Mutation

changed Histidine 1146 and Glutamic Acid 1148 to …

Promoter

CMV

Available Since

Nov. 5, 2012

Availability

Academic Institutions and Nonprofits only
mPlum-pBAD

Plasmid

#54564

Purpose

Localization: Protein Expression Vector , Excitation: 590, Emission: 649

Depositor

Michael Davidson, Roger Tsien

Article

Wang et al Proc Natl Acad Sci U S A. 2004 Nov 30;10

Type

Empty backbone

Tags

6xHis and mPlum

Expression

Bacterial

Available Since

July 25, 2014

Availability

Academic Institutions and Nonprofits only

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H2BK120C

Plasmid

#75332

Purpose

Bacteria expression of H2BK120C

Depositor

Cynthia Wolberger

Article

Morgan et al Science. 2016 Feb 12;351(6274):725-8. do

Insert

H2BK120C (h2bc11.S Frog)

Tags

none

Expression

Bacterial

Mutation

truncated; K120C

Promoter

T7

Available Since

May 16, 2016

Availability

Academic Institutions and Nonprofits only

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SpyLigase

Plasmid

#51722

Purpose

Plasmid for recombinant expression in bacteria of SpyLigase. The Howarth lab has created two improved peptide ligation enzymes. See depositor comments below!

Depositor

Mark Howarth

Article

Fierer et al Proc Natl Acad Sci U S A. 2014 Mar 17.

Insert

SpyLigase

Tags

His6 Tag

Expression

Bacterial

Promoter

T7

Available Since

May 1, 2014

Availability

Academic Institutions and Nonprofits only

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