We narrowed to 165,343 results for: addgene

1. pAc5.1B-HA-SBP-MBP_J

   Plasmid

   #146623

   Purpose

   Insect Expression vector

   Depositor

   Elisa Izaurralde

   Type

   Empty backbone

   Expression

   Insect

   Available Since

   Jan. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pT7-EGFP-SBP-C1_J

   Plasmid

   #146655

   Purpose

   Mammalian Expression vector

   Depositor

   Elisa Izaurralde

   Type

   Empty backbone

   Expression

   Mammalian

   Available Since

   Jan. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pT7-HA-SBP-C1_J

   Plasmid

   #146656

   Purpose

   Mammalian Expression vector

   Depositor

   Elisa Izaurralde

   Type

   Empty backbone

   Expression

   Mammalian

   Available Since

   Jan. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pT7-MS2-HA-C1_AA

   Plasmid

   #148246

   Purpose

   Mammalian Expression vector

   Depositor

   Elisa Izaurralde

   Type

   Empty backbone

   Expression

   Mammalian

   Available Since

   Nov. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pILGFP1DFB

   Plasmid

   #185871

   Purpose

   A parental plasmid for in vivo gene amplification at RPL25 locus

   Depositor

   Claudia Vickers

   Type

   Empty backbone

   Expression

   Yeast

   Mutation

   None

   Available Since

   Sept. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAc5.1C-RLuc-GFP52_D

   Plasmid

   #146099

   Purpose

   Insect Expression of GFP52

   Depositor

   Elisa Izaurralde

   Insert

   GFP52
   

   Expression

   Insect

   Available Since

   Feb. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. PL2x2_5

   Plasmid

   #45491

   Depositor

   David Baker

   Insert

   PL2x2_5
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   changed E30Y of Di-IV_5_PL2x2_6

   Available Since

   May 23, 2013

   Availability

   Academic Institutions and Nonprofits only

8. ninaC-15

   Plasmid

   #25889

   Depositor

   Gerald Rubin

   Insert

   p174
   

   Use

   Stratagene

   Expression

   Bacterial

   Available Since

   Aug. 4, 2010

   Availability

   Academic Institutions and Nonprofits only

9. lentiCRISPR v2

   Plasmid

   #52961

   Purpose

   Replaces original lentiCRISPRv1 (Addgene Plasmid 49535) and produces ~10-fold higher titer virus. 3rd generation lentiviral backbone.

   Depositor

   Feng Zhang

   Article

   Sanjana et al Nat Methods. 2014 Aug;11(8):783-4. doi:

   Inserts

   Cas9
   Puromycin resistance
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   EFS-NS

   Available Since

   May 8, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. CAG-GFP-IRES-CRE

    Plasmid

    #48201

    Purpose

    retroviral vector encoding for GFP and Cre-recombinase

    Depositor

    Fred Gage

    Article

    Zhao et al J Neurosci. 2006 Jan 4. 26(1):3-11.

    Inserts

    eGFP
    Cre
    

    Use

    Retroviral

    Expression

    Mammalian

    Promoter

    CAG and linked by IRES

    Available Since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. L4440_BioBrick-sgRNA

    Plasmid

    #177783

    Purpose

    Overexpression of sgRNAs in E. coli HT115, sgRNA overexpression scaffold; for ingestion by C. elegans

    Depositor

    Michael Ristow

    Article

    Fischer et al J Biol Chem. 2022 May 27:102085. doi: 10

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    June 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSpCas9(BB)-2A-Puro (PX459)

    Plasmid

    #48139

    Purpose

    NOTE: A new version of this plasmid is now available. See Addgene plasmid 62988.

    Depositor

    Feng Zhang

    Article

    Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

    Insert

    hSpCas9-2A-Puro
    

    Use

    CRISPR

    Tags

    2A-Puro and 3XFLAG

    Expression

    Mammalian

    Promoter

    Cbh

    Available Since

    Oct. 29, 2013

    Availability

    Academic Institutions and Nonprofits only

13. pIVT-Cas9-TMV

    Plasmid

    #238016

    Purpose

    for DNA-free gene knockout editing in rice and wheat or other plants

    Depositor

    Caixia Gao

    Article

    Qiu et al Plant Biotechnol J. 2025 Apr;23(4):1348-

    Insert

    SpCas9
    

    Expression

    Plant

    Promoter

    T7

    Available Since

    Oct. 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. LRG (Lenti_sgRNA_EFS_GFP)

    Plasmid

    #65656

    Purpose

    Lentiviral introduction of sgRNA constitute expression linked with GFP marker into mammalian cell line.

    Depositor

    Christopher Vakoc

    Article

    Shi et al Nat Biotechnol. 2015 May 11. doi: 10.103

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6 promoter-driven sgRNA expression and EFS promo…

    Available Since

    June 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCW-Flag-CtIP

    Plasmid

    #71108

    Purpose

    mammalian expession of Flag-CtlP

    Depositor

    Daniel Durocher

    Article

    Orthwein et al Nature. 2015 Dec 9. doi: 10.1038/nature1

    Insert

    CtlP
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Please see Depositor Comments

    Promoter

    TRE promoter, Tet ON

    Available Since

    Nov. 11, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. pSpCas9-2A-Puro-TBK1-gRNA (PX459)

    Plasmid

    #221550

    Purpose

    Expresses Cas9 and gRNA for disruption of TBK1 gene in human cells

    Depositor

    Shawn Ferguson

    Article

    Talaia et al bioRxiv [Preprint]. 2023 Dec 17:2023.12.

    Insert

    gRNA targeting human TBK1 (TBK1 Human)
    

    Expression

    Mammalian

    Promoter

    u6

    Available Since

    July 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCW-Flag-CtIP-T847E

    Plasmid

    #71110

    Purpose

    mammalian expession of Flag-CtlP T847E mutation

    Depositor

    Daniel Durocher

    Article

    Orthwein et al Nature. 2015 Dec 9. doi: 10.1038/nature1

    Insert

    CtlP
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    T847E

    Promoter

    TRE promoter, Tet ON

    Available Since

    Dec. 9, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. pAAV-G12-CheRiff-T2A-mCherry

    Plasmid

    #170275

    Purpose

    CheRiff-T2A-mCherry construct expression in mammlian cells. pAAV vector. Reporter plasmid.

    Depositor

    Vladislav Verkhusha

    Article

    Kaberniuk et al Nat Commun. 2021 Jun 23;12(1):3859. doi:

    Insert

    CheRiff-T2A-mCherry
    

    Use

    AAV

    Promoter

    11xUAS with TATA-box minimal promoter

    Available Since

    June 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-U6-sgRNA-CMV-GFP

    Plasmid

    #85451

    Purpose

    Express sgRNA in mammalian cells

    Depositor

    Hetian Lei

    Article

    Duan et al J Biol Chem. 2016 May 31. pii: jbc.M116.

    Insert

    pCMV-EGFP
    

    Use

    AAV

    Promoter

    pCMV-EGFP

    Available Since

    Jan. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. ColorfulCell

    Plasmid

    #62449

    Purpose

    MXS_chaining vector with 6 cassettes

    Depositor

    Pierre Neveu

    Article

    Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

    Inserts

    CMV::GTS-Citrine-bGHpA
    CMV::Lyn-Ceruleanx3-bGHpA
    CMV::NLS-TagBFPx3-bGHpA
    CMV::MTS-AzamiGreen-bGHpA
    CMV::iRFP670-SKL-bGHpA
    CMV::ETS-mCherry-KDEL-bGHpA
    

    Use

    Synthetic Biology

    Available Since

    May 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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