We narrowed to 3,807 results for: golden gate

1. pM-mOrange2

   Plasmid

   #228627

   Purpose

   Mammalian expression of mOrange2 and extra insert, for MuSIC barcode construction

   Depositor

   Marc Birtwistle

   Article

   Lu et al ACS Synth Biol. 2025 May 16;14(5):1533-1

   Insert

   mOrange2
   

   Expression

   Mammalian

   Mutation

   We introduced some silent mutations to avoid esse…

   Promoter

   CMV

   Available Since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pM-mRuby3

   Plasmid

   #228628

   Purpose

   Mammalian expression of mRuby3 and extra insert, for MuSIC barcode construction

   Depositor

   Marc Birtwistle

   Article

   Lu et al ACS Synth Biol. 2025 May 16;14(5):1533-1

   Insert

   mRuby3
   

   Expression

   Mammalian

   Mutation

   We introduced some silent mutations to avoid esse…

   Promoter

   CMV

   Available Since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pUC-hU6-crCoV-Mutiplex_EF1a-BFP

   Plasmid

   #224788

   Purpose

   SARS-COV-2 targeting crRNA array for RfxCas13d expressed from single hU6 promoter and reporter BFP protein expressed from EF1a promoter

   Depositor

   Gabor Balazsi

   Article

   Wan et al ACS Synth Biol. 2024 Oct 18;13(10):3212-

   Insert

   crCoV20, crCoV21, crCoV24
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Mammalian

   Promoter

   hU6 and hU6-2xTetO

   Available Since

   Oct. 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. FgH1tUTG-sgRNA.PRDM1-ZEB2_CRISPRd

   Plasmid

   #216171

   Purpose

   Express the gRNA targeting the PRDM1-binding site in the human ZEB2 locus for the CRISPRd experiment

   Depositor

   Makiko Iwafuchi

   Article

   Matsui et al Mol Cell. 2024 Jan 4:S1097-2765(23)01026

   Insert

   sgRNA- PRDM1.bs- ZEB2.locus (PRDM1 Human)
   

   Use

   Lentiviral

   Tags

   EGFP

   Available Since

   July 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pX330_NPM2_cterm1

   Plasmid

   #222911

   Purpose

   Cas9/sgRNA plasmid for targeting NPM2

   Depositor

   George Church

   Article

   Pierson Smela et al bioRxiv 2022.07.11.499564

   Insert

   Cas9, NPM2 sgRNA 1 (NPM2 Human, Synthetic)
   

   Use

   CRISPR

   Available Since

   July 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pX330_NPM2_cterm4

   Plasmid

   #222914

   Purpose

   Cas9/sgRNA plasmid for targeting NPM2

   Depositor

   George Church

   Article

   Pierson Smela et al bioRxiv 2022.07.11.499564

   Insert

   Cas9, NPM2 sgRNA 4 (NPM2 Human, Synthetic)
   

   Use

   CRISPR

   Available Since

   July 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pX330_NPM2_cterm3

   Plasmid

   #222913

   Purpose

   Cas9/sgRNA plasmid for targeting NPM2

   Depositor

   George Church

   Article

   Pierson Smela et al bioRxiv 2022.07.11.499564

   Insert

   Cas9, NPM2 sgRNA 3 (NPM2 Human, Synthetic)
   

   Use

   CRISPR

   Available Since

   July 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pX330_NPM2_cterm2

   Plasmid

   #222912

   Purpose

   Cas9/sgRNA plasmid for targeting NPM2

   Depositor

   George Church

   Article

   Pierson Smela et al bioRxiv 2022.07.11.499564

   Insert

   Cas9, NPM2 sgRNA 2 (NPM2 Human, Synthetic)
   

   Use

   CRISPR

   Available Since

   July 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. Gln-tRNA-gRNA scaffold for library 1&2

   Plasmid

   #192367

   Purpose

   tRNA sequence provider (for checkpoint and immune library)

   Depositor

   Lijia Ma

   Article

   Lu et al Nucleic Acids Res. 2022 Jun 24;50(11):65

   Insert

   Gln tRNA-gRNA scaffold
   

   Use

   For golden gate assembly

   Available Since

   Oct. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pNHT7

    Plasmid

    #186753

    Purpose

    A basic cloning vector for inserting genes with 5' and 3' UTRs for later subcloning via Golden Gate assembly.

    Depositor

    Bo Wang

    Article

    Hall et al Cell Rep Methods. 2022 Sep 20;2(10):1002

    Type

    Empty backbone

    Use

    Synthetic Biology; Cloning backbone

    Promoter

    T7

    Available Since

    Oct. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pYPQ131-RZ-Cas12j2

    Plasmid

    #173927

    Purpose

    Golden Gate entry vector; empty vector to clone the 1st Cas12j2 crRNA with ribozyme processing

    Depositor

    Yiping Qi

    Article

    Liu et al Plant Commun. 2022 Sep 20:100453. doi: 1

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Plant

    Available Since

    Oct. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pYPQ132-RZ-Cas12j2

    Plasmid

    #173928

    Purpose

    Golden Gate entry vector; empty vector to clone the 2nd Cas12j2 crRNA with ribozyme processing

    Depositor

    Yiping Qi

    Article

    Liu et al Plant Commun. 2022 Sep 20:100453. doi: 1

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Plant

    Available Since

    Oct. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pYPQ133-RZ-Cas12j2

    Plasmid

    #173929

    Purpose

    Golden Gate entry vector; empty vector to clone the 3rd Cas12j2 crRNA with ribozyme processing

    Depositor

    Yiping Qi

    Article

    Liu et al Plant Commun. 2022 Sep 20:100453. doi: 1

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Plant

    Available Since

    Oct. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pYPQ134-RZ-Cas12j2

    Plasmid

    #173930

    Purpose

    Golden Gate entry vector; empty vector to clone the 4th Cas12j2 crRNA with ribozyme processing

    Depositor

    Yiping Qi

    Article

    Liu et al Plant Commun. 2022 Sep 20:100453. doi: 1

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Plant

    Available Since

    Oct. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pSB139 - pL0_ZCT1 (CDS1)

    Plasmid

    #123184

    Purpose

    Golden Gate (MoClo; CDS1) compatible ZCT1 gene from Catharanthus roseus; a repressor of the STR1 promoter from C. roseus

    Depositor

    Carolyn Lee-Parsons, Erin Cram

    Article

    Mortensen et al Front Plant Sci. 2019 Jun 11;10:755. doi

    Insert

    ZCT1 from Catharanthus roseus
    

    Use

    Part for plant expression

    Expression

    Plant

    Mutation

    No BpiI and BsaI sites

    Available Since

    May 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pSB142 - pL0_ORCA3 (CDS1)

    Plasmid

    #123185

    Purpose

    Golden Gate (MoClo; CDS1) compatible ORCA3 gene from Catharanthus roseus; an activator of the STR1 promoter from C. roseus

    Depositor

    Carolyn Lee-Parsons, Erin Cram

    Article

    Mortensen et al Front Plant Sci. 2019 Jun 11;10:755. doi

    Insert

    ORCA3 from Catharanthus roseus
    

    Use

    Part for plant expression

    Expression

    Plant

    Mutation

    No BpiI and BsaI sites

    Available Since

    May 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pSB62 - pL0_STR (pro + 5U)

    Plasmid

    #123186

    Purpose

    Golden Gate (MoClo; PRO + 5U) compatible STR1 promoter from Catharanthus roseus

    Depositor

    Carolyn Lee-Parsons, Erin Cram

    Article

    Mortensen et al Front Plant Sci. 2019 Jun 11;10:755. doi

    Insert

    Catharanthus roseus STR1 promoter and 5'UTR
    

    Use

    Part for plant expression

    Expression

    Plant

    Mutation

    No BpiI and BsaI sites

    Available Since

    May 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pBEST-Pl-tetO1-tetR-linker-deGFP-T500 (109p)

    Plasmid

    #70195

    Purpose

    Post cloned 4-piece from Isothermal/Golden Gate assembly (GGA), using sequences from 2 (PltetO1-UTR1), 3 (tetR+linker from primer extension), 21 (linker from primer extension + deGFP), and 105 (vector

    Depositor

    Richard Murray

    Article

    Sun et al ACS Synth Biol. 2014 Jun 20;3(6):387-97.

    Insert

    tetR-deGFP fusion
    

    Available Since

    May 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pNP3425 [pNP2922 - ISCro4 Enhanced bRNA (TBL4, WT + DBL3, WT) + ISCro4 Recombinase (WT)]

    Plasmid

    #247262

    Purpose

    Express ISCro4 recombinase and bridge RNA

    Depositor

    Patrick Hsu

    Article

    Perry et al Science. 2025 Sep 25:eadz0276. doi: 10.1

    Insert

    pNP2922 - ISCro4 bRNA1-179 C60U, A61G, U73C, dU86, A87G, dU88, ins179(rc(101-111) (TBL4+DBL3)
    

    Expression

    Mammalian

    Mutation

    C60U, A61G, U73C, dU86, A87G, dU88, ins179(rc(101…

    Available Since

    Oct. 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pSLQ14901

    Plasmid

    #239276

    Purpose

    Expresses gG1 for pertubing endogenous human GAPDH mRNA via CRISPR-TO

    Depositor

    Stanley Qi

    Article

    Han et al Nature. 2025 Jul;643(8070):241-251. doi:

    Insert

    PspCas13b-hGAPDH-gRNA1 (GAPDH Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    Sept. 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge