We narrowed to 87,390 results for: lias

1. pCyterm-mScarlet-I3-NCwt_N1

   Plasmid

   #189779

   Purpose

   Construct for testing oligimerization of mScarlet-I3-NCwt in mammalian cells by OSER assay

   Depositor

   Dorus Gadella

   Article

   Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

   Insert

   Cyterm-mScarlet-I3-NCwt
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pHLmMBP-1

   Plasmid

   #72343

   Purpose

   Mammalian expression of secreted mMBP-fused proteins with C-terminal 6His-tag/Strep-tag II/HA-tag

   Depositor

   Luca Jovine

   Article

   Bokhove et al J Struct Biol. 2016 Feb 3. 194:1-7. pii

   Type

   Empty backbone

   Use

   Tags

   6His-tag, HA-tag, Strep-tag II, and mMBP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV enhancer + chicken beta-actin promoter

   Available since

   Feb. 17, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCS2-Lifeact-mRuby

   Plasmid

   #194339

   Purpose

   Expresses Lifeact with mRuby which labels F-actin in mammalian cells and zebrafish embryos

   Depositor

   Heidi Hehnly

   Article

   Aljiboury et al PLoS Genet. 2023 May 15;19(5):e1010765.

   Insert

   Lifeact
   

   Use

   Tags

   mRuby

   Expression

   Mammalian

   Mutation

   Promoter

   CMV IE94

   Available since

   Jan. 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pphiLOV3-N1

   Plasmid

   #178973

   Purpose

   Expresses phiLOV3 in mammalian cells

   Depositor

   Kiryl Piatkevich

   Article

   Babakhanova et al Protein Sci. 2022 Mar;31(3):728-751. doi

   Insert

   phiLOV3
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pKMD106e - intein-Cas9(S219)

   Plasmid

   #64190

   Purpose

   Expresses intein-Cas9(S219) in mammalian cells

   Depositor

   David Liu

   Article

   Davis et al Nat Chem Biol. 2015 May;11(5):316-8. doi

   Insert

   mammalian codon-optimized S. pyogenes intein-Cas9(S219) - NLS - 3x FLAG
   

   Use

   CRISPR

   Tags

   NLS - 3x FLAG (C terminal on insert)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. STIMATE-mCherry

   Plasmid

   #70676

   Purpose

   Expresses STIMATE or TMEM110 in mammalian cells

   Depositor

   Yubin Zhou

   Article

   Jing et al Nat Cell Biol. 2015 Oct;17(10):1339-47.

   Insert

   STIMATE (TMEM110 Human)
   

   Use

   Tags

   mcherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 30, 2015

   Availability

   Academic Institutions and Nonprofits only

7. B2375_CRY2olig-mEGFP

   Plasmid

   #215109

   Purpose

   Expresses CRY2olig (E490G CRY2 1-498) fused to monomeric EGFP in mammalian cells

   Depositor

   Chandra Tucker

   Article

   Hernandez-Candia et al Nat Chem Biol. 2024 Jan 8. doi: 10.1038/

   Insert

   CRY2olig-mEGFP (CRY2 Mustard Weed)
   

   Use

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   E490G, 1-498

   Promoter

   CMV

   Available since

   March 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCMV-CMP-PE-V1

   Plasmid

   #202769

   Purpose

   Expresses CMP-PE-V1 in mammalian cells for prime editing

   Depositor

   Kyoungmi Kim

   Article

   Park et al Genome Biol. 2021 Jun 3;22(1):170. doi:

   Insert

   CMP-PE-V1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pEGFP-Akt(K179M)

   Plasmid

   #39535

   Depositor

   Julian Downward

   Article

   Watton et al Curr Biol. 1999 Apr 22;9(8):433-6.

   Insert

   Akt(K179M) (Akt1 Mouse)
   

   Use

   Tags

   6G linker, EGFP, and HA

   Expression

   Mammalian

   Mutation

   K179M

   Promoter

   Available since

   Sept. 26, 2012

   Availability

   Academic Institutions and Nonprofits only

10. pYJ34-PB-CRISPR-BANCR-E1-gRNA

    Plasmid

    #131077

    Purpose

    long non-coding RNA BANCR knock out

    Depositor

    Xiaojun Lian

    Article

    Jiang et al Bioact Mater. 2022 Feb 7;14:313-320. doi

    Insert

    BANCR Exon1 gRNA (BANCR Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pIVT/Smarca4

    Plasmid

    #122146

    Purpose

    For making Smarca4 RNA

    Depositor

    Carmen Williams

    Article

    Miao et al Biol Reprod. 2018 Jan 9. pii: 4794936. d

    Insert

    Smarca4 (Smarca4 Mouse)
    

    Use

    For in vitro transcription

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    March 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMO746

    Plasmid

    #117480

    Purpose

    upp in an artificial operon with npt from pMO9071 and AmpR-pUC ori from pCR4/TOPO, Pnpt-npt-upp;, KanR

    Depositor

    Judy Wall

    Article

    Parks et al Science. 2013 Mar 15;339(6125):1332-5. d

    Insert

    uracil phosphoribosyltransferase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pJZC43

    Plasmid

    #66565

    Purpose

    sgRNA + 2XPP7 with PCP-VP64 effector for mammalian cells, marked by mCherry

    Depositor

    Wendell Lim, Stanley Qi

    Article

    Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

    Inserts

    sgRNA + 2XPP7
    PCP-VP64 IRES mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and U6

    Available since

    July 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCAGGS-3xFLAG-Rbfox2

    Plasmid

    #213726

    Purpose

    Mammalian cell expression vector for overexpression of Rbfox2 in motor neurons.

    Depositor

    Chaolin Zhang

    Article

    Jacko et al Neuron. 2018 Feb 21;97(4):853-868.e6. do

    Insert

    Rbfox2 (Rbfox2 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pHLmMBP-6

    Plasmid

    #72346

    Purpose

    Mammalian expression of cytoplasmic mMBP-fused proteins with C-terminal 6His-tag/Strep-tag II/HA-tag

    Depositor

    Luca Jovine

    Article

    Bokhove et al J Struct Biol. 2016 Feb 3. 194:1-7. pii

    Type

    Empty backbone

    Use

    Tags

    6His-tag, HA-tag, Strep-tag II, and mMBP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV enhancer + chicken beta-actin promoter

    Available since

    Feb. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Cry2L348F(1-531)-mCh-BAXS184E

    Plasmid

    #117239

    Purpose

    Light activated recruitment of BAX in mammalian cells with Cry2/CIB optogenetic switch

    Depositor

    Robert Hughes

    Article

    Godwin et al J Biol Chem. 2019 Nov 8;294(45):16918-16

    Insert

    Cry2531.L348F-mCh-BAXS184E
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. GFP-PKI WT

    Plasmid

    #118300

    Purpose

    Expression of PKIa

    Depositor

    Philip Stork

    Article

    Billiard et al J Biol Chem. 2001 Aug 17;276(33):31238-4

    Insert

    PKI wt
    

    Use

    Tags

    Flag and GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. GFP-GLT1a

    Plasmid

    #162515

    Purpose

    Rat GLT-1a cDNA N-terminally tagged with EGFP

    Depositor

    Josef Kittler

    Article

    Al Awabdh et al Glia. 2016 Jul;64(7):1252-64. doi: 10.10

    Insert

    GLT1a (Slc1a2 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. U6-3' MS2-CircRNA

    Plasmid

    #213751

    Purpose

    For circular RNA-mediated prime editor using U6-3' MS2-CircRNA in HEK293T cells

    Depositor

    Caixia Gao

    Article

    Liang et al Nat Biotechnol. 2024 Jan 10. doi: 10.103

    Insert

    5' ribozyme, 5' ligation sequences, 3' MS2, 3' ligation sequences, 3' ribozyme
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Feb. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. GFP-GLT1b

    Plasmid

    #162516

    Purpose

    Rat GLT-1b cDNA N-terminally tagged with EGFP

    Depositor

    Josef Kittler

    Article

    Al Awabdh et al Glia. 2016 Jul;64(7):1252-64. doi: 10.10

    Insert

    GLT1b (Slc1a2 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge