We narrowed to 13,079 results for: CAR

1. EGFP-Mm_MyoGEF(WT)

   Plasmid

   #136325

   Purpose

   Mammalian expression of mouse MyoGEF-WT with N-terminal EGFP

   Depositor

   Marcos Malumbres, Guillermo de Cárcer

   Article

   de Carcer et al Nat Med. 2017 Aug;23(8):964-974. doi: 10

   Insert

   PLEKHG6 (Plekhg6 Mouse)
   

   Expression

   Mammalian

   Available Since

   Aug. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAD96_hIRG1_4-461_pvp008

   Plasmid

   #124874

   Purpose

   Expresses hCAD Asn152Ser mutant in E.coli

   Depositor

   Konrad Buessow

   Article

   Chen et al Proc Natl Acad Sci U S A. 2019 Sep 23. p

   Insert

   cis-aconitate decarboxylase (ACOD1 Human)
   

   Tags

   StrepTag and TEV site

   Expression

   Bacterial

   Mutation

   mutated Asn152 to Ser, deleted amino acids 1-3 an…

   Promoter

   T7

   Available Since

   Oct. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCpep-E1GFP

   Plasmid

   #233884

   Purpose

   Expression of a pH sensitive variant of EGFP inserted in the C-peptide of insulin for pH measurement in insulin secretory granules

   Depositor

   Francesco Cardarelli

   Article

   De Lorenzi et al Commun Biol. 2025 Feb 25;8(1):304. doi:

   Insert

   Proinsulin-E1GFP (Ins2 Mouse)
   

   Expression

   Mammalian

   Mutation

   Inserted E1GFP into C-peptide of mouse Ins2 codin…

   Promoter

   CMVF

   Available Since

   April 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. Str-KDEL_SBP-EGFP-LAMP1-Y414A

   Plasmid

   #222319

   Purpose

   Synchronize the trafficking of LAMP1-Y414A from the ER.

   Depositor

   Franck Perez

   Article

   Ecard et al Mol Biol Cell. 2024 Mar 1;35(3):ar42. do

   Insert

   Streptavidin-KDEL and LAMP1-Y414A fused to SBP-EGFP (LAMP1 Human)
   

   Expression

   Mammalian

   Mutation

   Tyr414 to Ala

   Promoter

   CMV

   Available Since

   Feb. 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. Str-KDEL_SBP-mCherry-LAMP1-Y414A

   Plasmid

   #222320

   Purpose

   Synchronize the trafficking of LAMP1-A17 from the ER.

   Depositor

   Franck Perez

   Article

   Ecard et al Mol Biol Cell. 2024 Mar 1;35(3):ar42. do

   Insert

   Streptavidin-KDEL and LAMP1-Y414A fused to SBP-mCherry (LAMP1 Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. macroH2A1.1-F192V-GFP (pc5114)

   Plasmid

   #223437

   Purpose

   Mutation phenylalanine 192 to valine (F192V) in macroH2A1.1-GFP

   Depositor

   Cristina Cardoso

   Article

   Arroyo et al Nucleic Acids Res. 2024 Aug 27:gkae734.

   Insert

   macroH2A1.1-F192V (Macroh2a1 Rat)
   

   Tags

   GFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pKM 1995

   Plasmid

   #217826

   Purpose

   Expresses 1554 bp fragment of HY4 gene in A. thaliana. This HY4 fragment shows high homology to the phr genes in prokaryotes.

   Depositor

   Aziz Sancar

   Article

   Malhotra et al Biochemistry. 1995 May 23;34(20):6892-9.

   Insert

   HY4 (CRY1 Mustard Weed)
   

   Tags

   Maltose-binding protein

   Expression

   Bacterial

   Available Since

   June 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pIG-852_HA-GD2-28z_TFAP4-HA

   Plasmid

   #207502

   Purpose

   This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

   Depositor

   Alexander Marson

   Article

   Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

   Insert

   TFAP4-HA, HA-GD2-28z_CAR (TFAP4 Human)
   

   Use

   CRISPR

   Available Since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMeCP2G pc1121

   Plasmid

   #167566

   Purpose

   The complete rat MeCp2 ORF is fused in frame of the NH2-terminus of the enhanced GFP (isolated from pEGFP-N1 vector; CLONTECH Laboratories, Inc.)

   Depositor

   Cristina Cardoso

   Article

   Brero et al J Cell Biol. 2005 Jun 6;169(5):733-43. d

   Inserts

   Methyl-CpG binding protein 2 (Mecp2 Rat)
   GFP
   

   Tags

   GFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1-kappa-myc-dK-2xG4S-TMst

    Plasmid

    #145766

    Purpose

    Expresses myc-dL5(E52K, L91S) low affinity Fluorogen Activating Protein (FAP) on the extracellular surface anchored to the mammalian cell plasma membrane with the PDGFR transmembrane domain.

    Depositor

    Marcel Bruchez

    Article

    Carpenter et al ACS Chem Biol. 2020 Aug 17. doi: 10.1021

    Insert

    kappa-myc-dK-2xG4S-TMst
    

    Expression

    Mammalian

    Mutation

    The dK FAP is dL5 with mutations at E50K, L89S, a…

    Promoter

    CMV

    Available Since

    Jan. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. Donor-CD55-NeoR

    Plasmid

    #153551

    Purpose

    Used as a donor vector for the targeted knock-in of a CD55 mutation. This plasmid carries a neomycin resistance gene cassette

    Depositor

    Hiroyuki Konishi

    Article

    Hyodo et al Cell Rep. 2020 Jan 28;30(4):1195-1207.e7

    Insert

    mutant CD55 (a 1415-bp fragment from intron 3, exon 4 and intron 4, with a 1-bp mutation in exon 4), divided into 5' and 3' arms
    

    Use

    AAV and Cre/Lox; Donor plasmid/targeting vector

    Tags

    N/A

    Mutation

    a 1-bp mutation in exon 4

    Promoter

    No promoter

    Available Since

    March 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. Donor-PIGA-NeoR

    Plasmid

    #153549

    Purpose

    Used as a donor vector for the targeted knock-in of a PIGA mutation. This plasmid carries a neomycin resistance gene cassette

    Depositor

    Hiroyuki Konishi

    Article

    Hyodo et al Cell Rep. 2020 Jan 28;30(4):1195-1207.e7

    Insert

    mutant PIGA (a 1,991-bp fragment from intron 5 and exon 6 with a 3-bp mutation in exon 6), divided into 5' and 3' arms
    

    Use

    AAV and Cre/Lox; Donor plasmid/targeting vector

    Tags

    N/A

    Mutation

    a 3-bp mutation in exon 6

    Promoter

    No promoter

    Available Since

    Dec. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pENTR-Mm_MyoGEF(T575A)

    Plasmid

    #136328

    Purpose

    Mouse MyoGEF-T575A mutant in Gateway pENTR

    Depositor

    Marcos Malumbres, Guillermo de Cárcer

    Article

    de Carcer et al Nat Med. 2017 Aug;23(8):964-974. doi: 10

    Insert

    PLEKHG6 (Plekhg6 Mouse)
    

    Use

    Gateway entry vector

    Mutation

    T575A

    Available Since

    Aug. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAD100_hIRG1_4-461_pvp008

    Plasmid

    #124875

    Purpose

    Expresses hCAD Arg273His mutant in E.coli

    Depositor

    Konrad Buessow

    Article

    Chen et al Proc Natl Acad Sci U S A. 2019 Sep 23. p

    Insert

    cis-aconitate decarboxylase (ACOD1 Human)
    

    Tags

    StrepTag and TEV site

    Expression

    Bacterial

    Mutation

    mutated Arg273 to His, deleted amino acids 1-3 an…

    Promoter

    T7

    Available Since

    Sept. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. 289aa ELL2

    Plasmid

    #127266

    Purpose

    For in vitro translation of shorter human ELL2 from Met1. Also contains Met133I, M1381I, M186I mutations.

    Depositor

    Christine Milcarek

    Article

    Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

    Insert

    NH2-ELL2 (Met133ILeu, M1381ILeu, M186ILeu) (ELL2 Human)
    

    Use

    In vitro translation

    Mutation

    Three Mets (133, 138, 186) to Ileu. Contains 289…

    Promoter

    T7

    Available Since

    July 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. HA tagged Xba ELL2 Met133, 138, 186 to Ileu

    Plasmid

    #127269

    Purpose

    For in vitro translation of ELL2 with with internal HA tag and Met133, 138, 186 Ileu

    Depositor

    Christine Milcarek

    Article

    Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

    Insert

    ELL2 Met133, 138, 186 to Ileu (ELL2 Human)
    

    Use

    In vitro translation

    Mutation

    Met133, 138, 186 to Ileu HA tag after M186

    Promoter

    T7

    Available Since

    July 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. HA tagged Sph ELL2 Met133, 138, 186 to Ileu

    Plasmid

    #127271

    Purpose

    For in vitro translation of human ELL2 with Met133, 138, 186 to Ileu with HA tag after 8th Met

    Depositor

    Christine Milcarek

    Article

    Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

    Insert

    ELL2 Met133, 138, 186 to Ileu (ELL2 Human)
    

    Use

    In vitro translation

    Mutation

    Met133, 138, 186 to Ileu with HA tag after 8th Met

    Promoter

    T7

    Available Since

    July 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. HA tagged mid ELL2 M133, 138, 186ILeu

    Plasmid

    #127276

    Purpose

    Expresses human ELL2 with M133, 138, 186ILeu to prevent internal Met initiation peptides and contains middle HA tag that doesn't disrupt function

    Depositor

    Christine Milcarek

    Article

    Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

    Insert

    ELL2 (ELL2 Human)
    

    Expression

    Mammalian

    Mutation

    M133, 138, 186 to Ileu, HA tag at XbaI after Met …

    Promoter

    pEF1a

    Available Since

    July 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBABE-hygro-2xMyc-Rac1-P29S

    Plasmid

    #128581

    Purpose

    For mammalian cell expression of P29S mutant murine RAC1 carrying 2x Myc epitope tag sequences at the N-terminus.

    Depositor

    Julian Downward

    Article

    Lionarons et al Cancer Cell. 2019 Jul 8;36(1):68-83.e9.

    Insert

    Rac1 (Rac1 Mouse)
    

    Use

    Retroviral

    Tags

    2xMyc epitope

    Expression

    Mammalian

    Mutation

    Changed Proline29 to Serine.

    Available Since

    Aug. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBABE-hygro-2xMyc-Rac1-Q61L

    Plasmid

    #128582

    Purpose

    For mammalian cell expression of Q61L mutant murine RAC1 carrying 2x Myc epitope tag sequences at the N-terminus.

    Depositor

    Julian Downward

    Article

    Lionarons et al Cancer Cell. 2019 Jul 8;36(1):68-83.e9.

    Insert

    Rac1 (Rac1 Mouse)
    

    Use

    Retroviral

    Tags

    2xMyc epitope

    Expression

    Mammalian

    Mutation

    Changed Glutamine61 to Leucine.

    Available Since

    Aug. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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