We narrowed to 4,722 results for: golden gate
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Plasmid#218397Purpose3-exon minigene, with neutral middle exon, hnRNPA1 motif-rich region in second intron, and AG>GG mutation in final 3' splice siteDepositorInsertModified CHO DHFR minigene
ExpressionMammalianPromotertetracycline-responsive promoterAvailable SinceMay 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pK7-g7g8-K8
Plasmid#218220PurposeAllows users to clone two Spacer sequences into an arrray of AtU6-promoter driven sgRNAsDepositorInsertAtU6 promoters and sgRNA scaffolds
UseCRISPRAvailable SinceMay 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pK3-g3g4-K4
Plasmid#218218PurposeAllows users to clone two Spacer sequences into an arrray of AtU6-promoter driven sgRNAsDepositorInsertAtU6 promoters and sgRNA scaffolds
UseCRISPRAvailable SinceMay 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pK5-g5g6-K6
Plasmid#218219PurposeAllows users to clone two Spacer sequences into an arrray of AtU6-promoter driven sgRNAsDepositorInsertAtU6 promoters and sgRNA scaffolds
UseCRISPRAvailable SinceMay 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAGIG_V5-PPP6C
Plasmid#92013PurposeExpresses V5-PPP6C, includes IRES-EmeraldDepositorAvailable SinceAug. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
IBMc233
Plasmid#161939PurposeCarries Golden Gate substitution insert for IBM with M86 intein. PPhlF as C-lobe promoterDepositorInsertpSB1A3-BbsI-M86N-T-cat-PPhlF-RiboJ-B32-M86C-SapI
ExpressionBacterialPromoterPPhlFAvailable SinceMarch 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
IBMc050
Plasmid#161938PurposeCarries Golden Gate substitution insert for IBM with M86 intein. Plux2 as C-lobe promoterDepositorInsertpSB1A3-BbsI-M86N-cat-Plux2-B32-M86C-SapI
ExpressionBacterialPromoterPlux2Available SinceMarch 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
IBMc318
Plasmid#161941PurposeCarries Golden Gate substitution insert for ER-LBD domain-insertion mappingDepositorInsertpSB1A3-BbsI-G-ER-LBD-S-SapI
ExpressionBacterialAvailable SinceMarch 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
L1T10 - T2PB/pUBQ10 (mTagBFP2, mCherry, NeonGreen)
Plasmid#219687PurposeLevel 1 History-dependent target, switch PhiC31 then Bxb1, pUBQ10 promoter, and output: mtagBFP2 then mCherry then NeonGreen (for transformation, Kan plant resistance, Kan bacteria resistance).DepositorInsertT2PB/pUBQ10 (mTagBFP2, mCherry, NeonGreen)
ExpressionPlantAvailable SinceJuly 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
L1T18 - T2PsB/p35S preswitched (mTagBFP2, mCherry, NeonGreen)
Plasmid#219690PurposeLevel 1 preswitched History-dependent target, switch with Bxb1, p35S promoter, and output: mCherry then NeonGreen (for transformation, Kan plant resistance, Kan bacteria resistance).DepositorInsertT2PsB/p35S (mTagBFP2, mCherry, NeonGreen)
ExpressionPlantAvailable SinceJuly 16, 2024AvailabilityAcademic Institutions and Nonprofits only