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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 2,603 results for: ubiquitin

Showing: 1731 - 1740 of 2603 results

  1. His8:MBP-tev-Arg-eΔK-Flv

    Plasmid
    #83396
    Purpose
    bacterial expression vector pVP16 containing a derivative of E. coli lacZ fused with E. coli flavin binding protein MioC to be used - as control - in N-end rule in vitro ubiquitination studies
    Depositor
    Nico Dissmeyer
    Insert
    flavin binding protein MioC
    Use
    Synthetic Biology
    Tags
    3xHA, 8xHis, and MBP
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 21, 2017
    Availability
    Academic Institutions and Nonprofits only
    83396-plasmid-map-sequence-id-175052

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  2. His8:MBP-tev-Phe-eΔK-Flv

    Plasmid
    #83395
    Purpose
    bacterial expression vector pVP16 containing a derivative of E. coli lacZ fused with E. coli flavin binding protein MioC to be used - as control - in N-end rule in vitro ubiquitination studies
    Depositor
    Nico Dissmeyer
    Insert
    flavin binding protein MioC
    Use
    Synthetic Biology
    Tags
    3xHA, 8xHis, and MBP
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 21, 2017
    Availability
    Academic Institutions and Nonprofits only
    83395-plasmid-map-sequence-id-175037

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  3. Nav1.5_1-2linker411-717_PPSYtoAASA1960-1996

    Plasmid
    #187732
    Purpose
    Express a chimera of the Nav1.5 linker between the first and second transmembrane domain with the motif surrounding PPSY; residues 1974-1977 PPSY mutated to AASA
    Depositor
    Sandra Gabelli
    Article
    Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi
    Insert
    Nav1.5 1,2linker residues 411-717 and Nav1.5 residues 1960-1996 PPSY mutated to AASA (SCN5A Human)
    Use
    Tags
    His-TEV
    Expression
    Bacterial
    Mutation
    deletion of residues 1-410 and 718-1959 and 1997-…
    Promoter
    Available since
    Oct. 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    187732-plasmid-map-sequence-id-372456

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  4. p663-UBC-V5-TurboID-V5-STOP_IDG-K

    Plasmid
    #158159
    Purpose
    Gateway destination clone of TurboID-V5 (as control) tagged with N-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    V5-TurboID-V5
    Use
    Lentiviral; Gateway destination
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    Ubiquitin
    Available since
    Aug. 31, 2020
    Availability
    Academic Institutions and Nonprofits only
    158159-plasmid-map-sequence-id-309970

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  5. p663-UBC-PM-TurboID-V5_IDG-K

    Plasmid
    #214455
    Purpose
    Gateway destination clone of PM-TurboID (plasma membrane control) tagged with C-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    PM-TurboID-V5
    Use
    Lentiviral; Gateway destination
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    Ubiquitin
    Available since
    Feb. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    214455-plasmid-map-sequence-id-423213

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  6. FUGW-APOE-E3-T2A-mCherry

    Plasmid
    #190808
    Purpose
    Express APOE E3 allele from Ubiquitin C promoter, co-expressing mCherry
    Depositor
    Ronald Hart
    Insert
    APOE (APOE Human)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    E3
    Promoter
    Ubi C
    Available since
    Sept. 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    190808-plasmid-map-sequence-id-372853

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  7. FUGW-APOE-E4-T2A-mCherry

    Plasmid
    #190809
    Purpose
    Express APOE E4 allele from Ubiquitin C promoter, co-expressing mCherry
    Depositor
    Ronald Hart
    Insert
    APOE (APOE Human)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    E4
    Promoter
    Ubi C
    Available since
    Oct. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    190809-plasmid-map-sequence-id-374463

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  8. FUGW-APOE-E2-T2A-mCherry

    Plasmid
    #190807
    Purpose
    Express APOE E2 allele from Ubiquitin C promoter, co-expressing mCherry
    Depositor
    Ronald Hart
    Insert
    APOE (APOE Human)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    E2
    Promoter
    Ubi C
    Available since
    Sept. 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    190807-plasmid-map-sequence-id-372851

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  9. p663-UBC-V5-miniTurbo-V5-STOP_IDG-K

    Plasmid
    #158158
    Purpose
    Gateway destination clone of miniTurbo-V5 (as control) tagged with N-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    V5-miniTurbo-V5
    Use
    Lentiviral; Gateway destination
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    Ubiquitin
    Available since
    Oct. 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    158158-plasmid-map-sequence-id-314579

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  10. p663-UBC-2xV5-TurboID-NES-STOP_IDG-K

    Plasmid
    #158162
    Purpose
    Gateway destination clone of TurboID-NES (as control) tagged with N-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    2xV5-TurboID-NES
    Use
    Lentiviral; Gateway destination
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    Ubiquitin
    Available since
    Oct. 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    158162-plasmid-map-sequence-id-314581

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Showing: 1731 - 1740 of 2603 results