We narrowed to 8,296 results for: construct

1. R26TV CAG LSL 2-5

   Plasmid

   #68412

   Purpose

   Rosa26 targeting vector backbone for use with GMAP

   Depositor

   Tyler Jacks

   Article

   Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

   Type

   Empty backbone

   Use

   Cre/Lox and Mouse Targeting; Gmap

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Feb. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. p3E-FRT-KanR-FRT pA

   Plasmid

   #82601

   Purpose

   3' entry vector containing FRT-flanked kanamycin resistance cassette (KanR) with pA for FLP-induced kanamycin resistance, drug selection

   Depositor

   Kryn Stankunas

   Article

   Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

   Insert

   FRT-Kan-FRT
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   None

   Available since

   Dec. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. MSCV Puro-CMV:GFP

   Plasmid

   #68485

   Purpose

   Retrovirus expressing GFP driven by CMV promoter

   Depositor

   Tyler Jacks

   Article

   Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

   Inserts

   Puromycin Resistance
   GFP
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and LTR

   Available since

   Feb. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCGN N-Ras wt

   Plasmid

   #14723

   Depositor

   Adrienne Cox

   Article

   Fiordalisi et al Methods Enzymol. 2001 . 332():3-36.

   Insert

   N-ras (NRAS Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 29, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEnt L1L3 EF1a-tTA-2

   Plasmid

   #26803

   Depositor

   Edward Hsiao

   Article

   Hsiao et al Stem Cell Res Ther. 2011 Mar 4. 2(2):11.

   Insert

   Elongation Factor 1 alpha promoter and tTA transgene
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 23, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRosa26 R1-ccdB-R2 RexNeo PI-SceI

   Plasmid

   #24418

   Depositor

   Edward Hsiao

   Article

   Hsiao et al Stem Cell Res Ther. 2011 Mar 4. 2(2):11.

   Insert

   
   

   Use

   Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 16, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV/3' Box_(GLuc)_TOP1

   Plasmid

   #68434

   Purpose

   Transient expression of the "TOP1" construct, targeting the GLuc reporter, in mammalian cells. CMV/3' Box expression backbone.

   Depositor

   John Rinn

   Article

   Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

   Insert

   TOP1 construct
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3-NP-V5-B/BRISBANE/60/2008

   Plasmid

   #187923

   Purpose

   Expresses C terminal V5 tagged nucleoprotein of Influenza B/Brisbane/60/2008 virus in mammalian cells

   Depositor

   Arindam Mondal

   Article

   Kedia et al Front Microbiol. 2022 May 25;13:868367.

   Insert

   Influenza B Nucleoprotein
   

   Use

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   CMV Promoter

   Available since

   Nov. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCk4_spacer

   Plasmid

   #136074

   Purpose

   Use when making even level constructs (L2,...) if pCk4 position (position 4) has no construct assigned

   Depositor

   Jim Haseloff

   Article

   Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

   Insert

   Odd spacer
   

   Use

   Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   BsaI/ SapI domesticated

   Promoter

   Available since

   Nov. 30, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pCk3_spacer

    Plasmid

    #136073

    Purpose

    Use when making even level constructs (L2,...) if pCk3 position (position 3) has no construct assigned

    Depositor

    Jim Haseloff

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    Odd spacer
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    BsaI/ SapI domesticated

    Promoter

    Available since

    Nov. 30, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pCRII-1.1-1.10a

    Plasmid

    #69589

    Purpose

    OT-II TCR a chain cDNA in pCRII

    Depositor

    Francis R. Carbone

    Article

    Barnden et al Immunol Cell Biol. 1998 Feb;76(1):34-40.

    Insert

    TCR alpha clone 1.1 (Tcra Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 24, 2015

    Availability

    Academic Institutions and Nonprofits only

12. pGBW-m4046887

    Plasmid

    #145730

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 S (surface glycoprotein) (S Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;6xHIS

    Expression

    Bacterial

    Mutation

    wt

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available since

    April 28, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. pCE27

    Plasmid

    #174405

    Purpose

    C. auris LEUpOUT NAT marker gRNA expression construct. Use with pCE35 CAS9 expression construct.

    Depositor

    Clarissa Nobile

    Article

    Ennis et al Microbiol Spectr. 2021 Dec 22;9(3):e0182

    Insert

    NAT 2 of 2, pSNR52, ADE2 gRNA, gRNA conserved, C. auris LEU2 2 of 2
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCE38

    Plasmid

    #174432

    Purpose

    C. auris LEUpOUT HYG marker CAS9 expression construct. Use with pCE41 gRNA expression construct.

    Depositor

    Clarissa Nobile

    Article

    Ennis et al Microbiol Spectr. 2021 Dec 22;9(3):e0182

    Insert

    C. auris LEU2 1 of 2, pENO1, Cas9, HYG 1 of 2
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCE41

    Plasmid

    #174433

    Purpose

    C. auris LEUpOUT HYG marker gRNA expression construct. Use with pCE38 CAS9 expression construct.

    Depositor

    Clarissa Nobile

    Article

    Ennis et al Microbiol Spectr. 2021 Dec 22;9(3):e0182

    Insert

    HYG 2 of 2, pSNR52, ADE2 gRNA, gRNA conserved, C. auris LEU2 2 of 2
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Dec. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCE35

    Plasmid

    #174409

    Purpose

    C. auris LEUpOUT NAT marker CAS9 expression construct. Use with pCE27 gRNA expression construct.

    Depositor

    Clarissa Nobile

    Article

    Ennis et al Microbiol Spectr. 2021 Dec 22;9(3):e0182

    Insert

    C. auris LEU2 1 of 2, pENO1, Cas9, NAT 1 of 2
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCsC_spacer

    Plasmid

    #136077

    Purpose

    Use when making odd level constructs (L3,...) if pCsC position (position 3) has no construct assigned

    Depositor

    Jim Haseloff

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    Even spacer
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    BsaI/ SapI domesticated

    Promoter

    Available since

    Nov. 30, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. pCsE_spacer

    Plasmid

    #136078

    Purpose

    Use when making odd level constructs (L3,...) if pCsE position (position 4) has no construct assigned

    Depositor

    Jim Haseloff

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    Even spacer
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    BsaI/ SapI domesticated

    Promoter

    Available since

    Nov. 30, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pTmpKmA1O4in

    Plasmid

    #176232

    Purpose

    A template plasmid for amplification of the cI-hok-neo cassette, which contains A1O4 promoter. The promoter is directed toward the cI gene.

    Depositor

    Sergey Sineoky

    Article

    Bubnov et al Nucleic Acids Res. 2022 Aug 26;50(15):89

    Insert

    cI of phage lambda, hok of the R1 plasmid, neo, hybrid PA1O4 promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGBW-m4046828

    Plasmid

    #145742

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 S (surface glycoprotein) (S Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;6xHIS

    Expression

    Bacterial

    Mutation

    Escherichia coli recode 1

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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