We narrowed to 4,353 results for: erf

1. pCXLE-dCas9GFP-shP53

   Plasmid

   #102906

   Purpose

   EBNA episome plasmid for CAG promoter constitutive expression of dCas9-GFP. Includes p53 shRNA expression cassette.

   Depositor

   Timo Otonkoski

   Article

   Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

   Insert

   dCas9GFP-shP53
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   D10A, H840A

   Promoter

   CAG

   Available Since

   July 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLenti_dNmeCas9

   Plasmid

   #120077

   Purpose

   dNmeCas9 with no sgRNA cassette lenti construct

   Depositor

   Erik Sontheimer

   Article

   Amrani et al Genome Biol. 2018 Dec 5;19(1):214. doi:

   Insert

   dNmeCas9
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Feb. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMD19T-psba1-Ppsba2-dCas9-SpR

   Plasmid

   #73220

   Purpose

   Contains dCas9 from S. pyogenes under constitutive promoter. Suicide vector inserts into psba1 site of Synechocystis. Carries spectinomycin resist. Recommend E. coli Copy cutter for propogation.

   Depositor

   Paul Hudson

   Article

   Yao et al ACS Synth Biol. 2015 Dec 28.

   Insert

   dCas9 from S. pyogenes
   

   Use

   Tags

   c-myc

   Expression

   Bacterial

   Mutation

   Silent mutation at bp 1341 A->C to remove an E…

   Promoter

   PpsbA2

   Available Since

   March 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. sspa-GCaMP6s

   Plasmid

   #67559

   Purpose

   short photoactivatable fluorescent calcium reporter

   Depositor

   Ehud Isacoff

   Article

   Berlin et al Nat Methods. 2015 Jul 13. doi: 10.1038/n

   Insert

   spa-GCaMP6s
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   K65T, I83V, G87S, Y92D, V115H, K118V, S187R, Y196…

   Promoter

   Available Since

   July 28, 2015

   Availability

   Academic Institutions and Nonprofits only

5. pCfB6679

   Plasmid

   #106158

   Purpose

   EasyCloneYALI system-based yeast integrative vector for markerfree integration via CRISPR/Cas9 to be used in combination with pCfB6638, integration into Yarrowia lipolytica chromosomal location IntE_4, USER site for cloning, amp resistance

   Depositor

   Irina Borodina

   Article

   Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   May 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. NK-1 GFP

   Plasmid

   #24508

   Depositor

   Ron Vale

   Article

   Case et al Cell. 1997 Sep 5. 90(5):959-66.

   Insert

   Drosophila NCD, human kinesin
   

   Use

   Tags

   GFP and His

   Expression

   Bacterial

   Mutation

   NK-1 is a chimera where the entire superfamily-co…

   Promoter

   Available Since

   June 30, 2010

   Availability

   Academic Institutions and Nonprofits only

7. NAX_CAGGS_CAS9_m4_EYFP

   Plasmid

   #167866

   Purpose

   PiggyBac compatible plasmid expressing dCas9

   Depositor

   Alejandro Chavez, Nan Cher Yeo

   Article

   Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

   Insert

   dCas9
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   D10A, D839A, H840A, N863A

   Promoter

   Available Since

   Feb. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAW-EGFP-4xlet-7 (pAW-AS27)

   Plasmid

   #40834

   Purpose

   miRNA sensor used in Drosophila cell lines

   Depositor

   Phillip Zamore

   Article

   Ameres et al Science. 2010 Jun 18;328(5985):1534-9.

   Insert

   Four target sites perfectly complementary to let-7
   

   Use

   Mirna reporter

   Tags

   EGFP

   Expression

   Insect

   Mutation

   Promoter

   Actin5C

   Available Since

   Sept. 4, 2013

   Availability

   Academic Institutions and Nonprofits only

9. pC0.017

   Plasmid

   #119621

   Purpose

   Level 0 Part. CDS

   Depositor

   Alistair McCormick

   Article

   Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

   Insert

   dCas9
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   bp 331 C to A , 1237 A to C, 1284 C to T, 2121 C …

   Promoter

   Available Since

   Aug. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMNK55 - piRNAi 21ur-1224 - cku-80

    Plasmid

    #159820

    Purpose

    piRNA-induced RNA interference (piRNAi) to bias double-strand repair toward homologous recombination

    Depositor

    Christian Froekjaer-Jensen

    Article

    Priyadarshini et al Nat Methods. 2022 Feb;19(2):187-194. doi

    Insert

    cku-80 piRNAi 21ur-1224
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Not applicable

    Promoter

    Available Since

    June 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMNK54 - piRNAi 21ur-1224 - him-5

    Plasmid

    #159818

    Purpose

    piRNA-induced RNA interference (piRNAi) to induce males in transgenic animals

    Depositor

    Christian Froekjaer-Jensen

    Article

    Priyadarshini et al Nat Methods. 2022 Feb;19(2):187-194. doi

    Insert

    him-5 piRNAi 21ur-1224
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Not applicable

    Promoter

    Available Since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMNK56 - piRNAi 21ur-1224 - him-8

    Plasmid

    #159819

    Purpose

    piRNA-induced RNA interference (piRNAi) to induce males in transgenic animals

    Depositor

    Christian Froekjaer-Jensen

    Article

    Priyadarshini et al Nat Methods. 2022 Feb;19(2):187-194. doi

    Insert

    him-8 piRNAi 21ur-1224
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Not applicable

    Promoter

    Available Since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLPC myc-mRAP1

    Plasmid

    #73136

    Purpose

    Expression of myc-tagged mRAP1

    Depositor

    Titia de Lange, Agnel Sfeir

    Article

    Sfeir et al Science. 2010 Mar 26;327(5973):1657-61.

    Insert

    Rap1 (Terf2ip Mouse)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCfB6681

    Plasmid

    #106157

    Purpose

    EasyCloneYALI system-based yeast integrative vector for markerfree integration via CRISPR/Cas9 to be used in combination with pCfB6637, integration into Yarrowia lipolytica chromosomal location IntE_3, USER site for cloning, amp resistance

    Depositor

    Irina Borodina

    Article

    Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    May 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. 4xHRE_MLP-sfGFP-CMV_dsRed Exp

    Plasmid

    #117403

    Purpose

    sfGFP expressed from hypoxia-inducible MLP promoter and dsRed expressed from consitutive CMV promoter

    Depositor

    Yvonne Chen

    Article

    Ede et al ACS Synth Biol. 2016 May 20;5(5):395-404

    Insert

    SuperFolder GFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTFAP2C.1.0-gDNA

    Plasmid

    #113792

    Purpose

    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor TFAP2C

    Depositor

    Kevin White

    Insert

    TFAP2C (TFAP2C Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET28a-ybbR-HIS-CBM-CohI

    Plasmid

    #58709

    Purpose

    Probing molecule to perform single molecule force spectroscopy experiments

    Depositor

    Hermann Gaub

    Article

    Otten et al Nat Methods. 2014 Sep 7. doi: 10.1038/nm

    Insert

    CBM-CohI
    

    Use

    Tags

    HIS, HRV 3C, and ybbR

    Expression

    Bacterial

    Mutation

    changed Alanine 2 to Cysteine in CBM domain

    Promoter

    T7

    Available Since

    Aug. 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA6-rbN1 S77A

    Plasmid

    #32945

    Depositor

    Edward Weinman

    Article

    Weinman et al Am J Physiol Renal Physiol. 2009 Dec;297

    Insert

    SLc9a3r1 (SLC9A3R1 rabbit)
    

    Use

    Tags

    T7 tag, his, and partial FLAG

    Expression

    Mammalian

    Mutation

    Change Serine77 to Alanine

    Promoter

    CMV

    Available Since

    Feb. 22, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcold-6xHis-HRV3Csite-HsPACT

    Plasmid

    #41097

    Depositor

    Phillip Zamore

    Article

    Fukunaga et al Cell. 2012 Oct 9. pii: S0092-8674(12)011

    Insert

    PACT (PRKRA Human)
    

    Use

    Tags

    6xHis-HRV3Csite

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Nov. 20, 2012

    Availability

    Academic Institutions and Nonprofits only

20. pcDNA6-rbN1 S77D

    Plasmid

    #32947

    Depositor

    Edward Weinman

    Article

    Weinman et al Am J Physiol Renal Physiol. 2009 Dec;297

    Insert

    slc9a3r1 (SLC9A3R1 rabbit)
    

    Use

    Tags

    T7 tag, his, and partial FLAG

    Expression

    Mammalian

    Mutation

    Serine 77 mutated to Aspartic Acid

    Promoter

    CMV

    Available Since

    Feb. 22, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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