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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 25,772 results for: Vit;

Showing: 3481 - 3500 of 25772 results

  5. UPRT-mCh-Nluc-P2A-neo-UPRT

    Plasmid
    #135015
    Purpose
    Expresses mCherry protein and a fusion protein of Nluc-neo inserting into Cryptosporidium parvum UPRT locus
    Depositor
    David Sibley
    Article
    Wilke et al Cell Host Microbe. 2019 Jun 18. pii: S19
    Inserts
    mCh-Nluc-P2A-neo
    UPRT 5'UTR
    UPRT 3'UTR
    Use
    Cryptosporidium expression
    Tags
    mCherry and Nluc-neo
    Expression
    Mutation
    Promoter
    Cryptosporidium actin and enolase
    Available since
    Jan. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    135015-plasmid-map-sequence-id-266476

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  7. Lyn-FLINC-EKAR1(TA)

    Plasmid
    #87708
    Purpose
    Negative-control mutant of Lyn-FLINC-EKAR1.
    Depositor
    Jin Zhang
    Article
    Mo et al Nat Methods. 2017 Apr;14(4):427-434. doi
    Insert
    Lyn-FLINC-EKAR1(TA)
    Use
    Tags
    N-terminal targeting sequence from Lyn kinase
    Expression
    Mammalian
    Mutation
    Target Thr residue in substrate domain mutated to…
    Promoter
    CMV
    Available since
    Feb. 22, 2018
    Availability
    Academic Institutions and Nonprofits only
    87708-plasmid-map-sequence-id-171092

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  8. pNOC_dfnCas12a_crRNA NC

    Plasmid
    #176256
    Purpose
    pNOC episomal plasmid harboring the dead version of humanized fnCas12a gene sequence tagged with Nlux and spacer sequence is replaced by the type IIS restriction site for endonuclease BaeI that can be
    Depositor
    John van der Oost
    Article
    Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-
    Insert
    humanized fnCas12a
    Use
    CRISPR, Luciferase, and Synthetic Biology ; Expre…
    Tags
    luciferase
    Expression
    Mutation
    D917A and E1006A mutations to inactivate the endo…
    Promoter
    Available since
    Oct. 28, 2021
    Availability
    Academic Institutions and Nonprofits only
    176256-plasmid-map-sequence-id-346577

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  16. pcDNA3-Par6-ExRai-CKAR2

    Plasmid
    #236101
    Purpose
    Subcellularly targeted enhanced excitation-ratiometric biosensor for monitoring Protein Kinase C activity in living cells for selective detection of aPKC activity within the Par complex.
    Depositor
    Jin Zhang
    Article
    Su et al Nat Chem Biol. 2024 Oct 11. doi: 10.1038
    Insert
    Par6C-ExRai-CKAR2 (PARD6A Human, Synthetic)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    May 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    236101-plasmid-map-sequence-id-473796

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  18. pCDNA3.1-SP-ALFA-lk-HiBiT-lk-ADGRL3-lk-HiBiT

    Plasmid
    #233064
    Purpose
    Expresses mouse ADGRL3 (UniProt Q80TS3-3). An N-terminal AFLA-tag (SRLEEELRRRLTE) and HiBiT tag (VSGWRLFKKIS) follow the endogenous ADGRL3 signal peptide. Also includes a C-terminal HiBiT tag.
    Depositor
    Jonathan Javitch
    Article
    Perry-Hauser et al J Biol Chem. 2025 Jan 9:108174. doi: 10.
    Insert
    ADGRL3 (Adgrl3 Mouse)
    Use
    Tags
    ALFA and HiBiT
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    233064-plasmid-map-sequence-id-464574

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  19. pCDNA3.1-SP-ALFA-lk-HiBiT-lk-ADGRL3-T923G

    Plasmid
    #233063
    Purpose
    Expresses cleavage-deficient mouse ADGRL3-T923G (UniProt Q80TS3-3). An N-terminal AFLA-tag (SRLEEELRRRLTE) and HiBiT tag (VSGWRLFKKIS) follow the endogenous ADGRL3 signal peptide.
    Depositor
    Jonathan Javitch
    Article
    Perry-Hauser et al J Biol Chem. 2025 Jan 9:108174. doi: 10.
    Insert
    ADGRL3 (Adgrl3 Mouse)
    Use
    Tags
    ALFA and HiBiT
    Expression
    Mammalian
    Mutation
    Threonine 923 to Glycine
    Promoter
    Available since
    March 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    233063-plasmid-map-sequence-id-464572

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  20. pUC57 mini-ZsGreen-P2A-FLAG-HA-Regnase-1 D141N

    Plasmid
    #222650
    Purpose
    For in vitro transcription of mouse Zc3h12a D141N mutant tagged with FLAG-HA at N-term
    Depositor
    Silvia Monticelli
    Article
    Bataclan et al Life Sci Alliance. 2024 Jun 3;7(8):e2024
    Insert
    Zc3h12a (Zc3h12a Mouse)
    Use
    In vitro transcription
    Tags
    FLAG and HA
    Expression
    Mutation
    mutated aspartic acid 141 to asparagine to abroga…
    Promoter
    T7
    Available since
    Oct. 8, 2024
    Availability
    Academic Institutions and Nonprofits only
    222650-plasmid-map-sequence-id-448254

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Showing: 3481 - 3500 of 25772 results