We narrowed to 3,807 results for: golden gate

1. L4864 IL-10Rb NTEVp chain, IL-10Ra CTEVp chain with ZF6a in MGEV

   Plasmid

   #244189

   Purpose

   Multigene vector for constitutive expression of mTagBFP2, IL-10Rb NTEVp chain with human IgG VH signal peptide, and IL-10Ra CTEVp chain with ZF6a

   Depositor

   Joshua Leonard

   Article

   Edelstein et al Nat Chem Biol. 2025 Aug 22:10.1038/s4158

   Insert

   mTagBFP2; IL-10Rb NTEVp chain with human IgG VH signal sequence; IL-10Ra CTEVp chain with ZF6 synTF
   

   Use

   Synthetic Biology

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   NTEVp_75S, CTEVp_190K

   Promoter

   hEF1a, CMV

   Available Since

   Oct. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. L4857 dsRedExpress2 reporter, VEGFR2 NTEVp, VEGFR1 CTEVp in PiggyBac Transposon Vector

   Plasmid

   #244187

   Purpose

   PiggyBac transposon vector for expression of dsRed-Express2 synTF promoter; constitutive expression of VEGFR2 NTEVp chain, VEGFR1 CTEVp chain, and mNeonGreen-P2A-PuroR selection marker

   Depositor

   Joshua Leonard

   Article

   Edelstein et al Nat Chem Biol. 2025 Aug 22:10.1038/s4158

   Insert

   dsRed-Express2 under synTF responsive promoter; VEGFR2 NTEVp chain with WT NTEVp; VEGFR1 CTEVp chain; mNeonGreen-P2A-PuroR
   

   Use

   Synthetic Biology

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   CTEVp_190K

   Promoter

   hEF1a

   Available Since

   Oct. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pX330_mouseH3.1

   Plasmid

   #244241

   Purpose

   Expresses SpCas9 and a sgRNA targeting the mouse histone H3.1 loci for knock-in.

   Depositor

   Kazuhiro Maeshima

   Article

   Minami et al Sci Adv. 2025 Mar 28;11(13):eadu8400. do

   Insert

   sgRNA targeting mouse histone H3.1 (H3c1 Mouse)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Oct. 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pX330_H3.2

   Plasmid

   #244239

   Purpose

   Expresses SpCas9 and a sgRNA targeting the human histone H3.2 loci for knock-in.

   Depositor

   Kazuhiro Maeshima

   Article

   Minami et al Sci Adv. 2025 Mar 28;11(13):eadu8400. do

   Insert

   sgRNA targeting human histone H3.2 (H3C14, H3C15 Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Oct. 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pKMW121_WT-Cas9_U6-entry

   Plasmid

   #244818

   Purpose

   Mammalian expression of WT Cas9 with Golden Gate-compatible sgRNA spacer cassette

   Depositor

   Jennifer Doudna

   Article

   Shi et al Mol Cell. 2025 May 1;85(9):1730-1742.e9.

   Insert

   SpyCas9 (WT)
   

   Use

   CRISPR

   Tags

   3xFLAG-SV40 NLS and Nucleoplasmin NLS

   Expression

   Mammalian

   Promoter

   CAG, U6

   Available Since

   Sept. 17, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pKMW493_U6-sgRNA-entry

   Plasmid

   #244823

   Purpose

   Mammalian expression of SpyCas9 single guide RNA with Golden Gate-compatible sgRNA spacer

   Depositor

   Jennifer Doudna

   Article

   Shi et al Mol Cell. 2025 May 1;85(9):1730-1742.e9.

   Insert

   SpyCas9 single guide RNA
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Sept. 17, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pKMW122_SpG-Cas9_U6-entry

   Plasmid

   #244819

   Purpose

   Mammalian expression of SpG Cas9 with Golden Gate-compatible sgRNA spacer cassette

   Depositor

   Jennifer Doudna

   Article

   Shi et al Mol Cell. 2025 May 1;85(9):1730-1742.e9.

   Insert

   SpG Cas9
   

   Use

   CRISPR

   Tags

   3xFLAG-SV40 NLS and Nucleoplasmin NLS

   Expression

   Mammalian

   Mutation

   D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R

   Promoter

   CAG, U6

   Available Since

   Sept. 17, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. LLP1011

   Plasmid

   #239933

   Purpose

   dCas9 without Stop codon as Level-0 part (Golden Gate cloning)

   Depositor

   Ryan Lister

   Article

   Khan et al Nat Biotechnol. 2025 Mar;43(3):416-430.

   Insert

   dCas9
   

   Use

   CRISPR and Synthetic Biology

   Mutation

   N/A

   Available Since

   Sept. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. LLP1004

   Plasmid

   #239926

   Purpose

   sgRNA compatible with TCTP-SynPro-07 and CaMV 35S-SynPro-12 as Level-0 part (Golden Gate cloning)

   Depositor

   Ryan Lister

   Article

   Khan et al Nat Biotechnol. 2025 Mar;43(3):416-430.

   Insert

   sgRNA
   

   Use

   Synthetic Biology

   Mutation

   N/A

   Available Since

   Sept. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. LLP1005

    Plasmid

    #239927

    Purpose

    sgRNA compatible with TCTP-SynPro-02 as Level-0 part (Golden Gate cloning)

    Depositor

    Ryan Lister

    Article

    Khan et al Nat Biotechnol. 2025 Mar;43(3):416-430.

    Insert

    sgRNA
    

    Use

    Synthetic Biology

    Mutation

    N/A

    Available Since

    Sept. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. LLP1006

    Plasmid

    #239928

    Purpose

    sgRNA compatible with TCTP-SynPro-02 as Level-0 part (Golden Gate cloning)

    Depositor

    Ryan Lister

    Article

    Khan et al Nat Biotechnol. 2025 Mar;43(3):416-430.

    Insert

    sgRNA
    

    Use

    Synthetic Biology

    Mutation

    N/A

    Available Since

    Sept. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. LLP994

    Plasmid

    #239916

    Purpose

    sgRNA-D compatible with TCTP-SynPro-01, TCTP-SynPro-16 and CaMV 35S-SynPro-11 as Level-0 part (Golden Gate cloning)

    Depositor

    Ryan Lister

    Article

    Khan et al Nat Biotechnol. 2025 Mar;43(3):416-430.

    Insert

    sgRNA-D
    

    Use

    Synthetic Biology

    Mutation

    N/A

    Available Since

    Sept. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. LLP1001

    Plasmid

    #239923

    Purpose

    sgRNA compatible with TCTP-SynPro-09 and CaMV 35S-SynPro-03 as Level-0 part (Golden Gate cloning)

    Depositor

    Ryan Lister

    Article

    Khan et al Nat Biotechnol. 2025 Mar;43(3):416-430.

    Insert

    sgRNA
    

    Use

    Synthetic Biology

    Mutation

    N/A

    Available Since

    Sept. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. LLP1002

    Plasmid

    #239924

    Purpose

    sgRNA compatible with TCTP-SynPro-09 and CaMV 35S-SynPro-10 as Level-0 part (Golden Gate cloning)

    Depositor

    Ryan Lister

    Article

    Khan et al Nat Biotechnol. 2025 Mar;43(3):416-430.

    Insert

    sgRNA
    

    Use

    Synthetic Biology

    Mutation

    N/A

    Available Since

    Sept. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. LLP993

    Plasmid

    #239915

    Purpose

    sgRNA-C compatible with TCTP-SynPro-01, TCTP-SynPro-17 and CaMV 35S-SynPro-11 as Level-0 part (Golden Gate cloning)

    Depositor

    Ryan Lister

    Article

    Khan et al Nat Biotechnol. 2025 Mar;43(3):416-430.

    Insert

    sgRNA-C
    

    Use

    Synthetic Biology

    Mutation

    N/A

    Available Since

    Sept. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. LLP995

    Plasmid

    #239917

    Purpose

    sgRNA-E compatible with TCTP-SynPro-04, TCTP-SynPro-06 and CaMV 35S-SynPro-03 as Level-0 part (Golden Gate cloning)

    Depositor

    Ryan Lister

    Article

    Khan et al Nat Biotechnol. 2025 Mar;43(3):416-430.

    Insert

    sgRNA-E
    

    Use

    Synthetic Biology

    Mutation

    N/A

    Available Since

    Sept. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. LLP996

    Plasmid

    #239918

    Purpose

    sgRNA compatible with TCTP-SynPro-05 and CaMV 35S-SynPro-15 as Level-0 part (Golden Gate cloning)

    Depositor

    Ryan Lister

    Article

    Khan et al Nat Biotechnol. 2025 Mar;43(3):416-430.

    Insert

    sgRNA
    

    Use

    Synthetic Biology

    Mutation

    N/A

    Available Since

    Sept. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. LLP997

    Plasmid

    #239919

    Purpose

    sgRNA compatible with TCTP-SynPro-05 and CaMV 35S-SynPro-07 as Level-0 part (Golden Gate cloning)

    Depositor

    Ryan Lister

    Article

    Khan et al Nat Biotechnol. 2025 Mar;43(3):416-430.

    Insert

    sgRNA
    

    Use

    Synthetic Biology

    Mutation

    N/A

    Available Since

    Sept. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. LLP999

    Plasmid

    #239921

    Purpose

    sgRNA compatible with TCTP-SynPro-08 and CaMV 35S-SynPro-04 as Level-0 part (Golden Gate cloning)

    Depositor

    Ryan Lister

    Article

    Khan et al Nat Biotechnol. 2025 Mar;43(3):416-430.

    Insert

    sgRNA
    

    Use

    Synthetic Biology

    Mutation

    N/A

    Available Since

    Sept. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. LLP998

    Plasmid

    #239920

    Purpose

    sgRNA compatible with TCTP-SynPro-06, TCTP-SynPro-13 and CaMV 35S-SynPro-10 as Level-0 part (Golden Gate cloning)

    Depositor

    Ryan Lister

    Article

    Khan et al Nat Biotechnol. 2025 Mar;43(3):416-430.

    Insert

    sgRNA
    

    Use

    Synthetic Biology

    Mutation

    N/A

    Available Since

    Sept. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge