We narrowed to 13,043 results for: SHI

1. pBM019

   Plasmid

   #128179

   Purpose

   Construct for generating stable Cas9-expressing Toxoplasma gondii

   Depositor

   Sebastian Lourido

   Article

   Markus et al mSphere. 2019 Jun 26;4(3). pii: 4/3/e003

   Inserts

   sgRNA#1
   SpCas9
   Chloramphenicol acetyltransferase
   

   Use

   CRISPR; Toxoplasma gondii expression

   Tags

   3X FLAG and Nuclear Localization Signal

   Expression

   Mutation

   Promoter

   TUB1 (Toxoplasma gondii), U6 (Toxoplasma gondii),…

   Available since

   July 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pH2R-mCherry-N1

   Plasmid

   #84326

   Purpose

   Histamine-2 receptor tagged with mCherry

   Depositor

   Dorus Gadella

   Article

   van Unen et al Mol Pharmacol. 2016 Sep;90(3):162-76. do

   Insert

   H2R (HRH2 Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHS0806 His14-bdSUMO-AwaIscB

   Plasmid

   #176539

   Purpose

   Bacterial expression of His14-bdSUMO-AwaIscB

   Depositor

   Feng Zhang

   Article

   Altae-Tran et al Science. 2021 Oct;374(6563):57-65. doi:

   Insert

   AwaIscB
   

   Use

   CRISPR

   Tags

   14xHis-bdSUMO tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMDJ22 - pEXP[Psmu-2 | Cre Recombinase (PATCs) | gpd-2 tagRFP-T smu-1 UTR]

   Plasmid

   #159814

   Purpose

   Optimized enzyme for genome editing in C. elegans

   Depositor

   Christian Froekjaer-Jensen

   Article

   Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

   Insert

   pEXP[Psmu-2 | Cre Recombinase (PATCs) | gpd-2 tagRFP-T smu-1 UTR]
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Not applicable

   Promoter

   Available since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pLVX-NPC1(P202A/F203A)-FLAG

   Plasmid

   #164976

   Purpose

   Lentiviral vector for NPC1 expression in mammalian cells

   Depositor

   Roberto Zoncu

   Article

   Davis et al Dev Cell. 2020 Dec 7. pii: S1534-5807(20

   Insert

   NPC1 (NPC1 Human)
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   P202A/ F203A

   Promoter

   EF1a

   Available since

   March 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEX-CFP-hLC3WT

   Plasmid

   #24985

   Depositor

   Isei Tanida

   Article

   Tanida et al Autophagy. 2008 Jan 1. 4(1):131-4.

   Insert

   a wild type human MAP1-LC3B fused with ECFP (MAP1LC3B Human)
   

   Use

   Tags

   ECFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 21, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. Human NANOS3-P2A-mCherry knock-in targeting construct

   Plasmid

   #59721

   Purpose

   Gene targeting construct for human NANOS3 locus, introducing a P2A-mCherry reporter gene. Contains an frt-PGK-Neo-PA-frt cassette that can be excised.

   Depositor

   Jacob Hanna

   Article

   Irie et al Cell. 2015 Jan 15;160(1-2):253-68. doi:

   Insert

   P2A mCherry
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 29, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMS_375

   Plasmid

   #182132

   Purpose

   expresses retron RNA, ec.86 Reverse Transcriptase, CspRecT SSAP, and mutL* to create a specific rifampicin resistance mutation while inhibiting MMR

   Depositor

   George Church

   Article

   Schubert et al Proc Natl Acad Sci U S A. 2021 May 4;118

   Inserts

   retron RNA
   ec.86 RT
   CspRecT
   

   Use

   Tags

   Expression

   Mutation

   incorporated rifampicin-resistance-conferring don…

   Promoter

   Available since

   Sept. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pMK312 (TOP2A CRISPR)

   Plasmid

   #140654

   Purpose

   TOP2A tagging CRISPR

   Depositor

   Masato Kanemaki

   Article

   Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

   Insert

   TOP2A (TOP2A Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAAV-Ef1a-fDIO-mRuby3-2A-dCre

    Plasmid

    #203845

    Purpose

    Flp-dependent enzymatically inactive Cre and mRuby3 expression

    Depositor

    Ivo Spiegel

    Article

    Roethler et al Neuron. 2023 Jun 20:S0896-6273(23)00399-

    Insert

    dCre
    

    Use

    AAV

    Tags

    mRuby3-2A

    Expression

    Mutation

    Enzymatically inactive Cre

    Promoter

    Available since

    Aug. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pX330.Pten.a

    Plasmid

    #66587

    Purpose

    sgRNA for Pten deletion

    Depositor

    Wen Xue

    Article

    Li et al Genome Biol. 2015 May 28;16(1):111.

    Insert

    Pten deletion (Pten Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 27, 2015

    Availability

    Academic Institutions and Nonprofits only

12. MS2_adRNA-MCP_ADAR1 DD (E1008Q)_NLS

    Plasmid

    #124626

    Purpose

    Expresses MS2_adRNA targeting the RAB7A transcript along with the MCP_ADAR1 DD(E1008Q)_NLS

    Depositor

    Prashant Mali

    Article

    Katrekar et al Nat Methods. 2019 Mar;16(3):239-242. doi

    Insert

    MCP_ADAR1 DD (E1008Q)_NLS
    

    Use

    AAV

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    E1008Q hyperactive mutant

    Promoter

    CMV

    Available since

    May 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAAV-hSyn-OsTIR1(WT)

    Plasmid

    #140729

    Purpose

    OsTIR1(WT)-P2A-mAID-EGFP-NES

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

    Insert

    OsTIR1(WT)-P2A-mAID-EGFP-NES
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. AsCas12a-2C-NLS in pCSDest

    Plasmid

    #126637

    Purpose

    Expresses AsCas12a-2C-NLS in mammalian cells

    Depositor

    Scot Wolfe

    Article

    Liu et al Nucleic Acids Res. 2019 Mar 20. pii: 540

    Insert

    AsCas12a-2C-NLS
    

    Use

    CRISPR

    Tags

    3xHuman influenza hemagglutinin (HA)-Tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV IE94 promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pHS1861

    Plasmid

    #205974

    Purpose

    FLAG-SV40 NLS-Cas8-HNH from JAGBLH010000220 Cas8-HNH in pcDNA3.1(+)

    Depositor

    Feng Zhang

    Article

    Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

    Insert

    Cas8-HNH
    

    Use

    Tags

    FLAG-SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. AAV_pSyn_Mito-LOV-Turbo-V5

    Plasmid

    #199669

    Purpose

    expresses Mito-LOV-Turbo-V5 in neurons, AAV vector

    Depositor

    Alice Ting

    Article

    Lee et al Nat Methods. 2023 May 15. doi: 10.1038/s

    Insert

    LOV-Turbo
    

    Use

    AAV

    Tags

    COX4I1 mito targeting sequence and V5

    Expression

    Mutation

    Promoter

    Synapsin

    Available since

    May 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pGEX Stat5A-SH2

    Plasmid

    #46515

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    Stat5A (STAT5A Human)
    

    Use

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 582-693

    Promoter

    Tac

    Available since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAsCpf1(TATV)(BB) (pY221)

    Plasmid

    #89354

    Purpose

    Expresses humanized AsCpf1 TATV PAM variant and crRNA guide

    Depositor

    Feng Zhang

    Article

    Gao et al Nat Biotechnol. 2017 Jun 5. doi: 10.1038

    Insert

    Cpf1 RVR variant
    

    Use

    CRISPR

    Tags

    NLS and NLS-3xHA

    Expression

    Mammalian

    Mutation

    S542R/K548V/N552R

    Promoter

    CBh

    Available since

    July 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLenti-eGFP-dSaCas9-blast

    Plasmid

    #191653

    Purpose

    Lentiviral expression of S. aureus dead Cas9 (dCas9/dSaCas9/SadCas9), eGFP, and the blasticidin resistance gene.

    Depositor

    Moshe Szyf

    Article

    Sapozhnikov et al Sci Rep. 2023 Feb 9;13(1):2280. doi: 10.

    Insert

    eGFP-T2A-dSaCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    3XFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pEX-GFP-hLC3ΔG

    Plasmid

    #24988

    Depositor

    Isei Tanida

    Article

    Tanida et al Autophagy. 2008 Jan 1. 4(1):131-4.

    Insert

    a mutant human MAP1-LC3B fused with EGFP (MAP1LC3B Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    deleted Gly at 121

    Promoter

    Available since

    June 28, 2010

    Availability

    Academic Institutions and Nonprofits only

21. pMSCV-FKBP-RFP(pLxIS-PLPLR)

    Plasmid

    #221284

    Purpose

    Expression of RFP (pLxIS-PLPLR) in mammalian cells by retroviral transduction

    Depositor

    Jonathan Kagan

    Article

    Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

    Insert

    RFP (pLxIS-PLPLR) (Mkrn1 Synthetic)
    

    Use

    Retroviral

    Tags

    3xFLAG-4xFKBP

    Expression

    Mutation

    Promoter

    Available since

    July 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pMSCV-FKBP-TRIF(Nterm)

    Plasmid

    #221244

    Purpose

    Expression of TRIF N-terminus (1-222) in mammalian cells by retroviral transduction

    Depositor

    Jonathan Kagan

    Article

    Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

    Insert

    TRIF N-terminus (1-222) (Ticam1 Mouse)
    

    Use

    Retroviral

    Tags

    3xFLAG-4xFKBP

    Expression

    Mutation

    Promoter

    Available since

    July 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. integrin beta6 pcDNA1 neo

    Plasmid

    #13580

    Depositor

    Dean Sheppard

    Article

    Weinacker et al J Biol Chem. 1994 Mar 4. 269(9):6940-8.

    Insert

    integrin beta 6 (ITGB6 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 8, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. 3xFLAG-CUL7-pCMV7.1

    Plasmid

    #155025

    Purpose

    N-terminal 3xFLAG-tagged CUL7 in a pCMV7.1 vector

    Depositor

    Andre Catic

    Article

    Sweeney et al Sci Rep. 2020 Aug 18;10(1):13942. doi: 1

    Insert

    Cullin 7 (CUL7 Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Bacterial

    Mutation

    Promoter

    pCMV7.1

    Available since

    Sept. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pGEX Stat5B-SH2

    Plasmid

    #46516

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    Stat5B (STAT5B Human)
    

    Use

    Tags

    AviTag, GST, and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 593-692

    Promoter

    Tac

    Available since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. PDE9A_pcDNA6.2/EmGFP-Bsd

    Plasmid

    #176945

    Purpose

    Mammalian expression vector encoding PDE9A and EmGFP-Bsd

    Depositor

    Roger Reeves

    Article

    Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

    Insert

    PDE9A (PDE9A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. CoV2-N-R203K

    Plasmid

    #177951

    Purpose

    Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (R203K). Used for generating RNA packaging virus-like particles.

    Depositor

    Jennifer Doudna

    Article

    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

    Insert

    SARS-CoV-2 Nucleocapsid (R203K) (N )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    R203K

    Promoter

    Available since

    Nov. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCFJ2475 - pEXP[Psmu-2 | mosase(PATCs) | gpd-2 tagRFP-T(myr, patcs) smu-1 UTR]

    Plasmid

    #159813

    Purpose

    Optimized enzyme for genome editing in C. elegans

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    pEXP[Psmu-2 | mosase(PATCs) | gpd-2 tagRFP-T(myr, patcs) smu-1 UTR]
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Not applicable

    Promoter

    Available since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCFJ2334 - [1-2] ENTR - Fluor - ce-GFP(PATCs(900bp), NLS, no_atg, no stop)

    Plasmid

    #159850

    Purpose

    Three-fragment Gateway compatible flourophore for expression in C. elegans

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    [1-2] ENTR - Fluor - ce-GFP(PATCs(900bp), NLS, no_atg, no stop)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Not applicable

    Promoter

    Available since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pEX-HcRed-hLC3WT

    Plasmid

    #24991

    Depositor

    Isei Tanida

    Article

    Tanida et al Autophagy. 2008 Jan 1. 4(1):131-4.

    Insert

    a wild type human MAP1-LC3B fused with HcRed (MAP1LC3B Human)
    

    Use

    Tags

    HcRed

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 21, 2010

    Availability

    Academic Institutions and Nonprofits only

31. EGFP-uTEV1-NES

    Plasmid

    #171006

    Purpose

    HiLITR protease with C-terminal NNES (Cytoplasmic)

    Depositor

    Alice Ting

    Article

    Coukos et al eLife. 2021 Aug 20;10. pii: 69142. doi:

    Insert

    EGFP-uTEV1-NES
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pTRE-tight (TetOn)

    Available since

    Aug. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. Sufu_pLENTI-CAG-IRES-GFP

    Plasmid

    #176993

    Purpose

    Mammalian lentiviral expression vector encoding Sufu

    Depositor

    Roger Reeves

    Article

    Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

    Insert

    Sufu (Sufu Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    CAG

    Available since

    April 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pHS1871

    Plasmid

    #205976

    Purpose

    U6-JAGBLH010000220 Cas8-HNH DR-BpiI-DR

    Depositor

    Feng Zhang

    Article

    Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

    Insert

    Cas8-HNH DR-BpiI-DR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Feb. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pGEX ShcD-SH2

    Plasmid

    #46488

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    ShcD (SHC4 Human)
    

    Use

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 512-623

    Promoter

    Tac

    Available since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pHLmMBP-2

    Plasmid

    #72344

    Purpose

    Mammalian expression of secreted N-terminally 6His-tagged mMBP-fused proteins with C-terminal 6His-tag/Strep-tag II/HA-tag

    Depositor

    Luca Jovine

    Article

    Bokhove et al J Struct Biol. 2016 Feb 3. 194:1-7. pii

    Type

    Empty backbone

    Use

    Tags

    6His-tag, HA-tag, Strep-tag II, and mMBP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV enhancer + chicken beta-actin promoter

    Available since

    Feb. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. p5071 pcDNA4c hBrd4 (595-1362)

    Plasmid

    #14450

    Depositor

    Peter Howley

    Article

    You et al Cell. 2004 Apr 30. 117(3):349-60.

    Insert

    Brd4 (595-1362) (BRD4 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    amino acids 595-1362

    Promoter

    Available since

    March 2, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. CMV:eGFP-p2A-HA-GPR151

    Plasmid

    #190133

    Purpose

    expresses eGFP-p2A-HA-GPR151 (wild-type variant) in mammalian cells

    Depositor

    Joshua Knowles

    Article

    Bielczyk-Maczynska et al Nat Commun. 2022 Dec 1;13(1):7408. doi:

    Insert

    eGFP-p2A-HA-GPR151
    

    Use

    Lentiviral

    Tags

    HA, eGFP, and p2A

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pGEX SH2-B-SH2

    Plasmid

    #46479

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    SH2-B (SH2B1 Human)
    

    Use

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 515-639

    Promoter

    Tac

    Available since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pHS1543

    Plasmid

    #205963

    Purpose

    NZ_CP025815 DinG HNH Cascade proteins operon (E. coli codon optimized) with His14-tagged Csf3 in pET45b(+)

    Depositor

    Feng Zhang

    Article

    Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

    Insert

    DinG HNH Cascade with His14-tagged Csf3
    

    Use

    Tags

    14xHis on Csf3

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pMDJ112 - pEXP(HygroR, 5605)[Phsp-16.41 | eFlp recombinase (PATCs) | tbb-2 UTR]

    Plasmid

    #159811

    Purpose

    Optimized enzyme for genome editing in C. elegans

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    pEXP(HygroR, 5605)[Phsp-16.41 | eFlp recombinase (PATCs) | tbb-2 UTR]
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Not applicable

    Promoter

    Available since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pClneoMyc human CIN85

    Plasmid

    #47935

    Purpose

    expresses Myc tagged human CIN85 in mammalian cells

    Depositor

    Yutaka Hata

    Article

    Kawata et al J Biochem. 2006 May;139(5):931-9.

    Insert

    CIN85 (SH3KBP1 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    contains amino acids 2-665 of human CIN85; D114G

    Promoter

    CMV

    Available since

    Sept. 27, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pGEX Stat6-SH2

    Plasmid

    #46517

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    Stat6 (STAT6 Human)
    

    Use

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 510-654

    Promoter

    Tac

    Available since

    July 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pHS1517

    Plasmid

    #205980

    Purpose

    FLAG-SV40 NLS-Cas5-HNH from MWEE01000013 Cas5-HNH in pcDNA3.1(+)

    Depositor

    Feng Zhang

    Article

    Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

    Insert

    Cas5-HNH
    

    Use

    Tags

    FLAG-SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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44. pEGFP-N2-RIPK1 I539D

    Plasmid

    #78848

    Purpose

    To detect localization of RIPK1 I539D protein in live mammalian cells

    Depositor

    Jaewhan Song

    Article

    Seo et al Nat Cell Biol. 2016 Mar;18(3):291-302. d

    Insert

    RIPK1 (RIPK1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    I539D

    Promoter

    CMV

    Available since

    June 14, 2016

    Availability

    Academic Institutions and Nonprofits only

45. pLenti-dSaCas9-KRAB-blast

    Plasmid

    #191654

    Purpose

    Lentiviral expression of S. aureus dead Cas9 (dCas9/dSaCas9/SadCas9) fused to the KRAB domain for gene silencing and the blasticidin resistance gene.

    Depositor

    Moshe Szyf

    Article

    Sapozhnikov et al Sci Rep. 2023 Feb 9;13(1):2280. doi: 10.

    Insert

    dSaCas9-KRAB
    

    Use

    CRISPR and Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. CoV2-N-G204R

    Plasmid

    #177953

    Purpose

    Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (G204R). Used for generating RNA packaging virus-like particles.

    Depositor

    Jennifer Doudna

    Article

    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

    Insert

    SARS-CoV-2 Nucleocapsid (G204R) (N )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    G204R

    Promoter

    Available since

    Nov. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. TIRAP-GFP

    Plasmid

    #52739

    Purpose

    Retroviral vector expresses WT hTIRAPfused to GFP. Also contains IRES-hCD2 as selectable marker

    Depositor

    Jonathan Kagan

    Article

    Bonham et al Cell. 2014 Feb 13;156(4):705-16. doi: 10

    Insert

    Toll/IL-1 Receptor adaptor protein (TIRAP Human)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 20, 2014

    Availability

    Academic Institutions and Nonprofits only

48. SMC2 CRISPR

    Plasmid

    #140649

    Purpose

    SMC2 tagging CRISPR

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

    Insert

    SMC2 (SMC2 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pLenti CRISPR sgMTHFD2_1

    Plasmid

    #106313

    Purpose

    Express Cas9 and sgRNA targeting MTHFD2

    Depositor

    Richard Possemato

    Article

    Minton et al Mol Cell. 2018 Feb 15;69(4):610-621.e5.

    Insert

    sgRNA targeting MTHFD2 (MTHFD2 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pAAV-CMV-SspB-EGFP-VP16-P2A-FLAG-Gal4DBD-CapN-SsrA-CapC-HA

    Plasmid

    #213536

    Purpose

    Expresses the split transcription factors in mouse liver

    Depositor

    Wenjing Wang

    Article

    Shen et al Nat Methods. 2023 Jan;20(1):112-122. doi

    Insert

    SspB-EGFP-VP16-P2A-FLAG-Gal4DBD-CapN-SsrA-CapC-HA-HA
    

    Use

    AAV

    Tags

    EGFP and HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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