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mScarlet-I-Myo10 ΔF

Plasmid

#145139

Purpose

Expresses mScarlet-I tagged MYO10 construct with a MyTH/FERM domain deletion

Depositor

Johanna Ivaska

Article

Miihkinen et al Cell Rep. 2021 Sep 14;36(11):109716. doi

Insert

MYO10 ΔF (MYO10 Human)

Expression

Mammalian

Available Since

July 2, 2025

Availability

Academic Institutions and Nonprofits only

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pENTR2b ITGB1-tail-mTurquoise2

Plasmid

#145142

Purpose

Shuttle vector with mTurquoise2-tagged ITGB1 cytoplasmic tail as an insert

Depositor

Johanna Ivaska

Article

Miihkinen et al Cell Rep. 2021 Sep 14;36(11):109716. doi

Insert

ITGB1-tail-mTurquoise2 (ITGB1 Human)

Use

Entry vector for gateway cloning

Available Since

July 2, 2025

Availability

Academic Institutions and Nonprofits only

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pENTR2b ITGA2-tail-YPet

Plasmid

#145143

Purpose

Shuttle vector with YPet-tagged ITGA2 wild-type cytoplasmic tail as an insert

Depositor

Johanna Ivaska

Article

Miihkinen et al Cell Rep. 2021 Sep 14;36(11):109716. doi

Insert

ITGA2-tail-YPet (ITGA2 Human)

Use

Entry vector for gateway cloning

Available Since

July 2, 2025

Availability

Academic Institutions and Nonprofits only

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pOP823

Plasmid

#235718

Purpose

Protein expression of His6-SUMO-HsRAB5A (Q79L C19S,C63S) 1-212 in bacteria for maleimide conjugation

Depositor

Roger Williams

Article

Buckles et al Biophys J. 2020 Dec 1;119(11):2205-2218.

Insert

RAB5A (RAB5A Human)

Tags

His6-SUMO

Expression

Bacterial

Mutation

Q79L, C19S, C63S, aa 1-212

Available Since

May 13, 2025

Availability

Academic Institutions and Nonprofits only

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pYO1645

Plasmid

#235719

Purpose

Protein expression of GST-tagged PX domain (Cys at 2 and C85S) in bacteria

Depositor

Roger Williams

Article

Buckles et al Biophys J. 2020 Dec 1;119(11):2205-2218.

Insert

p40phox PX domain (NCF4 Human)

Tags

GST

Expression

Bacterial

Mutation

Cys at 2, C85S, aa 2-149

Available Since

April 16, 2025

Availability

Academic Institutions and Nonprofits only

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EHMT1_exon 3_gRNA

Plasmid

#228809

Purpose

CRISPR targeting of human EHMT1

Depositor

Shravanti Rampalli

Article

Biligiri et al PLOS Biology 22(11): e3002871.

Insert

gRNA targeting EHMT1 exon 3 (EHMT1 Human)

Use

CRISPR

Expression

Mammalian

Available Since

March 10, 2025

Availability

Academic Institutions and Nonprofits only

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EGFP_EHMT1B1-4

Plasmid

#228812

Purpose

Expression of mouse EHMT1 with deletion of aa 809-1298

Depositor

Shravanti Rampalli

Article

Biligiri et al PLOS Biology 22(11): e3002871.

Insert

EHMT1 (Ehmt1 Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

deletion of aa 809-1298, See Depositor Comments

Available Since

March 10, 2025

Availability

Academic Institutions and Nonprofits only

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pGG-PIP39

Plasmid

#196777

Purpose

Contains WRKY11 promoter

Depositor

Marc Somssich

Article

Calabria et al J Exp Bot. 2025 Jan 17:erae516. doi: 10.

Insert

WRKY DNA-BINDING PROTEIN 11 (WRKY11 Mustard Weed)

Use

Greengate (gg) entry vector

Mutation

240 C->G

Available Since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a_PA1472_Cterm_His

Plasmid

#220485

Purpose

heterologous expression of PA1472 for protein purification

Depositor

Emily Balskus

Article

Mayers et al Cell. 2024 Jun 11:S0092-8674(24)00579-8.

Insert

PA1472

Tags

6xHs

Expression

Bacterial

Mutation

none

Available Since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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pgRNA-bacteria_speG_M15

Plasmid

#220488

Purpose

inducible CRISPRi

Depositor

Emily Balskus

Article

Mayers et al Cell. 2024 Jun 11:S0092-8674(24)00579-8.

Insert

gspeG

Use

CRISPR and RNAi

Mutation

none

Available Since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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pgRNA-bacteria_speG_E23

Plasmid

#220489

Purpose

inducible CRISPRi

Depositor

Emily Balskus

Article

Mayers et al Cell. 2024 Jun 11:S0092-8674(24)00579-8.

Insert

gspeG

Use

CRISPR and RNAi

Mutation

none

Available Since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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pTrcHis_VectorA_short_speG_genomic

Plasmid

#220473

Purpose

heterologous expression under endogenous speG E. coli promoter

Depositor

Emily Balskus

Article

Mayers et al Cell. 2024 Jun 11:S0092-8674(24)00579-8.

Insert

speG

Expression

Bacterial

Mutation

none

Available Since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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pTrcHis_VectorA_short_speGpromoter_PA4114

Plasmid

#220474

Purpose

heterologous expression of PA4114 under endogenous speG E. coli promoter

Depositor

Emily Balskus

Article

Mayers et al Cell. 2024 Jun 11:S0092-8674(24)00579-8.

Insert

PA4114

Expression

Bacterial

Mutation

none

Available Since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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pTrcHis_VectorA_short_speGpromoter_PA1472

Plasmid

#220475

Purpose

heterologous expression of PA1472 under endogenous speG E. coli promoter

Depositor

Emily Balskus

Article

Mayers et al Cell. 2024 Jun 11:S0092-8674(24)00579-8.

Insert

PA1472

Expression

Bacterial

Mutation

none

Available Since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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pTrcHis_VectorA_short_speGpromoter_PA1377

Plasmid

#220476

Purpose

heterologous expression of PA1377 under endogenous speG E. coli promoter

Depositor

Emily Balskus

Article

Mayers et al Cell. 2024 Jun 11:S0092-8674(24)00579-8.

Insert

PA1377

Expression

Bacterial

Mutation

none

Available Since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a_speG_Nterm_His

Plasmid

#220478

Purpose

heterologous expression for protein purification

Depositor

Emily Balskus

Article

Mayers et al Cell. 2024 Jun 11:S0092-8674(24)00579-8.

Insert

speG

Tags

6xHs

Expression

Bacterial

Mutation

none

Available Since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a_PA4114_Nterm_His

Plasmid

#220479

Purpose

heterologous expression of PA4114 for protein purification

Depositor

Emily Balskus

Article

Mayers et al Cell. 2024 Jun 11:S0092-8674(24)00579-8.

Insert

PA4114

Tags

6xHs

Expression

Bacterial

Mutation

none

Available Since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a_PA1472_Nterm_His

Plasmid

#220480

Purpose

heterologous expression of PA1472 for protein purification

Depositor

Emily Balskus

Article

Mayers et al Cell. 2024 Jun 11:S0092-8674(24)00579-8.

Insert

PA1472

Tags

6xHs

Expression

Bacterial

Mutation

none

Available Since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a_PA1377_Nterm_His

Plasmid

#220481

Purpose

heterologous expression of PA1377 for protein purification

Depositor

Emily Balskus

Article

Mayers et al Cell. 2024 Jun 11:S0092-8674(24)00579-8.

Insert

PA1377

Tags

6xHs

Expression

Bacterial

Mutation

none

Available Since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-BBS9

Plasmid

#218717

Purpose

Expresses N-terminally EGFP-tagged BBS9 in mammalian cells

Depositor

Kazuhisa Nakayama

Article

Katoh et al J Cell Sci. 2015 May 11. pii: jcs.168740

Insert

BBS9 (BBS9 Human)

Tags

EGFP

Expression

Mammalian

Mutation

A455T (g1876a)

Promoter

CMV

Available Since

June 14, 2024

Availability

Academic Institutions and Nonprofits only

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