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UBC-TLR4-HA hygro

Plasmid

#246905

Purpose

Expresses HA-tagged human TLR4 in mammalian cells

Depositor

William Kaelin

Article

Lampson et al Cell. 2024 Apr 25;187(9):2209-2223.e16.

Insert

TLR4 (TLR4 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Promoter

UBC

Available Since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pDEST-ITGB1-V1

Plasmid

#215454

Purpose

Expression vector with integrin beta1 tagged with the BiFC V1 fragment

Depositor

Johanna Ivaska

Article

Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

Insert

ITGB1 (ITGB1 Human)

Tags

Venus fragment 1 (V1)

Expression

Mammalian

Mutation

Silent mutations added to disrupt shRNA binding a…

Promoter

CMV

Available Since

Nov. 3, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pCS2+8-CYP2A6(C14A, C82A, C439A)-flag-tev-halo-his

Plasmid

#227164

Purpose

For T-REX experiments of CYP2A6 C14A, C82A, and C439A triple mutant. Almost no sensing ability to reactive electrophilic species (RES).

Depositor

Yimon Aye

Article

Liu et al Cell. 2024 Dec 26;187(26):7450-7469.e29.

Insert

CYP2A6 (CYP2A6 Human)

Tags

Flag, HaloTag, and His6

Expression

Mammalian

Mutation

changed cysteine 14, cysteine 82, and cysteine 43…

Promoter

CMV and SP6

Available Since

Aug. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pENTR2b-ITGB1(WT)

Plasmid

#215450

Purpose

Gateway entry vector with integrin beta1 wild-type (WT)

Depositor

Johanna Ivaska

Article

Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

Insert

ITGB1 (ITGB1 Human)

Use

Gateway entry vector

Mutation

Silent mutations added to disrupt shRNA binding a…

Promoter

none

Available Since

May 29, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA5-FRT/TO-LaG16-APT1

Plasmid

#234423

Purpose

Express APT1 with the anti-GFP nanobody LaG16 fused at the N terminus

Depositor

William Fuller

Article

Kuo et al Nat Commun. 2025 Feb 7;16(1):1445. doi:

Insert

APT1 fused to LaG16 (LYPLA1 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

April 25, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT/TO-APT1-LaG16

Plasmid

#234420

Purpose

Express APT1 with the anti-GFP nanobody LaG16 fused at the C terminus separated by a flexible linker

Depositor

William Fuller

Article

Kuo et al Nat Commun. 2025 Feb 7;16(1):1445. doi:

Insert

APT1 fused to LaG16 (LYPLA1 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

April 25, 2025

Availability

Academic Institutions and Nonprofits only

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AAV 9P31-hSyn-Venus-P2A-Tau-RING I18R/M72E

Plasmid

#233694

Purpose

AAV expression of Venus-P2A-Tau-RING-I18R/M72E from hSyn promoter

Depositor

Leo James

Article

Miller et al Cell. 2024 Oct 17;187(21):5967-5980.e17.

Insert

Venus-P2A-Tau-RING I18R-M72E

Use

AAV

Expression

Mammalian

Available Since

April 18, 2025

Availability

Academic Institutions and Nonprofits only

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TFORF0897

Plasmid

#142707

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

ING1 (ING1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Feb. 27, 2025

Availability

Academic Institutions and Nonprofits only

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Str-KDEL_SBP-EGFP-LAMP1-Y414A

Plasmid

#222319

Purpose

Synchronize the trafficking of LAMP1-Y414A from the ER.

Depositor

Franck Perez

Article

Ecard et al Mol Biol Cell. 2024 Mar 1;35(3):ar42. do

Insert

Streptavidin-KDEL and LAMP1-Y414A fused to SBP-EGFP (LAMP1 Human)

Expression

Mammalian

Mutation

Tyr414 to Ala

Promoter

CMV

Available Since

Feb. 4, 2025

Availability

Academic Institutions and Nonprofits only

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Str-KDEL_SBP-mCherry-LAMP1-Y414A

Plasmid

#222320

Purpose

Synchronize the trafficking of LAMP1-A17 from the ER.

Depositor

Franck Perez

Article

Ecard et al Mol Biol Cell. 2024 Mar 1;35(3):ar42. do

Insert

Streptavidin-KDEL and LAMP1-Y414A fused to SBP-mCherry (LAMP1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pMVP-SEC24C

Plasmid

#231559

Purpose

pMVP expression vector for human SEC24C (wild type, closed, resistant to sgSEC24C #1 and #2) (Blasticidin selection marker)

Depositor

Rizwan Haq

Article

Schiantarelli et al Cancer Cell. 2025 Feb 10;43(2):308-316.e

Insert

SEC24C (SEC24C Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

silent PAM mutations (G67 (GGG>GGA) and A115 (…

Promoter

CMV

Available Since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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PD2529 human beta1 ecto

Plasmid

#221398

Purpose

Mammalian expression of human integrin beta1 ectodomain

Depositor

Timothy Springer

Article

Li et al Cell. 2024 May 14:S0092-8674(24)00475-6.

Insert

integrin beta1 ectodomain (ITGB1 Human)

Tags

AVI tag, HRV3C cleavage site, basic coil, HA tag,…

Expression

Mammalian

Mutation

codon optimized for human mature _1 residues Q1 t…

Available Since

Sept. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pHR-CLIP-CD28(CD45TM)

Plasmid

#223629

Purpose

Lentiviral expression of human CD28 (CD45TM) with CLIP tag in mammalian cells

Depositor

Enfu Hui

Article

Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

Insert

CD28 (CD28 Human)

Use

Lentiviral; mammalian expression

Tags

CLIP

Mutation

CD28 transmembrane domain replaced by CD45 transm…

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pHR-Spy-CD28(CD45TM)

Plasmid

#223624

Purpose

Lentiviral expression of human CD28 (CD45TM) with SpyTag003 in mammalian cells

Depositor

Enfu Hui

Article

Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

Insert

CD28 (CD28 Human)

Use

Lentiviral; mammalian expression

Tags

Spytag003

Mutation

CD28 transmembrane domain replaced by CD45 transm…

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pHR-SNAP-CD86(F56A)

Plasmid

#223609

Purpose

Lentiviral expression of human CD86 (F56A) with SNAP tag in mammalian cells

Depositor

Enfu Hui

Article

Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

Insert

CD86 (CD86 Human)

Use

Lentiviral; mammalian expression

Tags

SNAP

Mutation

F56A

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pHR-SNAP-CD80(Y65A)

Plasmid

#223608

Purpose

Lentiviral expression of human CD80 (Y65A) with SNAP tag in mammalian cells

Depositor

Enfu Hui

Article

Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

Insert

CD80 (CD80 Human)

Use

Lentiviral; mammalian expression

Tags

SNAP

Mutation

Y65A

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pHR-CLIP-CD28(SGGG)

Plasmid

#223607

Purpose

Lentiviral expression of human CD28 (SGGG) with CLIP tag in mammalian cells

Depositor

Enfu Hui

Article

Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

Insert

CD28 (CD28 Human)

Use

Lentiviral; mammalian expression

Tags

CLIP

Mutation

MYPPPY mutated to SGGG

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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Beta1 Q280* K418* full-length

Plasmid

#221406

Purpose

Mammalian expression of human integrin beta1 Q280* K418* full-length

Depositor

Timothy Springer

Article

Li et al Cell. 2024 May 14:S0092-8674(24)00475-6.

Insert

integrin beta1 Q280* K418* full-length (ITGB1 Human)

Tags

CD33 secretion peptide (MPLLLLLPLLWAGALA) and HA …

Expression

Mammalian

Mutation

codon-optimized mature sequence

Available Since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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Beta1 N391* N562* full-length

Plasmid

#221407

Purpose

Mammalian expression of human integrin beta1 N391* N562* full-length

Depositor

Timothy Springer

Article

Li et al Cell. 2024 May 14:S0092-8674(24)00475-6.

Insert

integrin beta1 N391* N562* full-length (ITGB1 Human)

Tags

CD33 secretion peptide (MPLLLLLPLLWAGALA) and HA …

Expression

Mammalian

Mutation

codon-optimized mature sequence

Available Since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV_FKBP-V5-PRRX1

Plasmid

#215024

Purpose

AAV vector for knocking in an N-terminal tag (FKBP12F36V and V5) for human PRRX1

Depositor

Joanna Wysocka

Article

Kim et al Cell. 2024 Feb 1;187(3):692-711.e26. doi

Insert

paired related homeobox 1 (PRRX1 Human)

Use

AAV

Tags

FKBP-V5

Available Since

May 16, 2024

Availability

Academic Institutions and Nonprofits only

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