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We narrowed to 4,953 results for: codon optimized

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Showing: 3501 - 3520 of 4953 results

  3. AAV2_hSyn_Aurora_CA_Citrine

    Plasmid
    #98219
    Purpose
    Slow cycling (open for minutes) step-function artificial anion conducting channelrhodopsin (aACR). High light sensitivity. Activation with green light, inactivation max with 625 nm (accelarates closure to ms). Not recommended for in vivo use. Codon optimized for mammalian expression.
    Depositor
    Simon Wiegert
    Publication
    Wietek et al Sci Rep. 2017 Nov 2;7(1):14957. doi: 10.
    Insert
    Synthetic construct Aurora_C128A gene
    Use
    AAV
    Tags
    Citrine
    Expression
    Mammalian
    Mutation
    V59S, E83N, E90Q, E101S, V117R, E123S, C128A, P24…
    Promoter
    human synapsin
    Available since
    Aug. 22, 2017
    Availability
    Academic Institutions and Nonprofits only
    98219-plasmid-map-sequence-id-256052

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  4. pETM6-1D4-mCherry

    Plasmid
    #73423
    Purpose
    Reporter plasmid encoding mCherry, codon optimized for E. coli, transcriptionally driven by orthogonal T7-lac promoter variant 1D4.
    Depositor
    Mattheos Koffas
    Publication
    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw
    Insert
    Promoter 1D4 (orthogonal T7-lac variant)
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    P1D4 (orthogonal T7-lac variant)
    Available since
    March 4, 2016
    Availability
    Academic Institutions and Nonprofits only
    73423-plasmid-map-sequence-id-135323

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  5. pETM6-4A6-mCherry

    Plasmid
    #73424
    Purpose
    Reporter plasmid encoding mCherry, codon optimized for E. coli, transcriptionally driven by orthogonal T7-lac promoter variant 4A6.
    Depositor
    Mattheos Koffas
    Publication
    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw
    Insert
    Promoter 4A6 (orthogonal T7-lac variant)
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    P4A6 (orthogonal T7-lac variant)
    Available since
    March 4, 2016
    Availability
    Academic Institutions and Nonprofits only
    73424-plasmid-map-sequence-id-135324

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  6. pETM6-1E4-mCherry

    Plasmid
    #73426
    Purpose
    Reporter plasmid encoding mCherry, codon optimized for E. coli, transcriptionally driven by orthogonal T7-lac promoter variant 1E4.
    Depositor
    Mattheos Koffas
    Publication
    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw
    Insert
    Promoter 1E4 (orthogonal T7-lac variant)
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    P1E4 (orthogonal T7-lac variant)
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    73426-plasmid-map-sequence-id-135326

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  7. pETM6-1B6-mCherry

    Plasmid
    #73420
    Purpose
    Reporter plasmid encoding mCherry, codon optimized for E. coli, transcriptionally driven by orthogonal T7-lac promoter variant 1B6.
    Depositor
    Mattheos Koffas
    Publication
    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw
    Insert
    Promoter 1B6 (orthogonal T7-lac variant)
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    P1B6 (orthogonal T7-lac variant)
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    73420-plasmid-map-sequence-id-135320

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  8. pETM6-3H5-mCherry

    Plasmid
    #73419
    Purpose
    Reporter plasmid encoding mCherry, codon optimized for E. coli, transcriptionally driven by orthogonal T7-lac promoter variant 3H5.
    Depositor
    Mattheos Koffas
    Publication
    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw
    Insert
    Promoter 3H5 (orthogonal T7-lac variant)
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    P3H5 (orthogonal T7-lac variant)
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    73419-plasmid-map-sequence-id-135319

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  10. prhaBAD-pA-Hia5

    Plasmid
    #222303
    Purpose
    Plasmid for rhamnose inducible expression and purification of protein A-Hia5-6XHis (pA-Hia5) for antibody directed DNA (adenine) methylation (as in DiMeLo-seq)
    Depositor
    Aaron Straight
    Publication
    Altemose et al Nat Methods. 2022 Jun;19(6):711-723. doi
    Insert
    pA-Hia5
    Tags
    6His and protein A
    Expression
    Bacterial
    Mutation
    codon optimized for bacterial expression
    Promoter
    rhaBAD
    Available since
    Oct. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    222303-plasmid-map-sequence-id-447746

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  11. Lenti-EFS-BirAopt-GSQ-RBXN-P2A-BLAST

    Plasmid
    #208048
    Purpose
    Enables constitutive expression of BirAopt alone; RBXN represents a EcoR1-BsiW1-Xba1-Not1 polylinker; to perform bioE3; selection with blasticidin
    Depositor
    Rosa Barrio, James Sutherland
    Publication
    Barroso-Gomila et al Nat Commun. 2023 Nov 23;14(1):7656. doi:
    Insert
    BirAopt
    Use
    Lentiviral
    Mutation
    BirAopt is human-codon-optimized BirA (PMID 15707…
    Promoter
    EFS
    Available since
    Nov. 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    208048-plasmid-map-sequence-id-410202

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  12. pLenti-nSpCas9-evoCDA1

    Plasmid
    #209042
    Purpose
    Lentiviral vector expressing doxycycline-inducible human codon-optimized cytosine base editor where evoCDA1 is fused to the N-terminus of SpCas9(D10A)-sDNA Rad51-2xUGI-6xNLS
    Depositor
    Thomas Gonatopoulos-Pournatzis
    Publication
    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e
    Insert
    evoCDA1-nSpCas9
    Use
    CRISPR and Lentiviral
    Tags
    FLAG-HA
    Expression
    Mammalian
    Mutation
    D10A
    Promoter
    Tight TRE promoter
    Available since
    June 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    209042-plasmid-map-sequence-id-413615

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  13. pYPQ230-STU

    Plasmid
    #138110
    Purpose
    Gateway entry plasmid (attL1 & attR5) expressing rice codon optimized LbCas12a without promoter and terminator
    Depositor
    Yiping Qi
    Publication
    Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:
    Insert
    LbCas12a
    Use
    CRISPR; Gateway compatible cas12a entry clone
    Tags
    NLS
    Expression
    Plant
    Available since
    March 31, 2021
    Availability
    Academic Institutions and Nonprofits only
    138110-plasmid-map-sequence-id-272040

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  14. pCJ202

    Plasmid
    #162675
    Purpose
    pET-21b(+) based plasmid for expression of PETase from Ideonella sakaiensis 201-F6 (Genbank GAP38373.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating W159C and S238C mutations.
    Depositor
    Gregg Beckham
    Publication
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    PETase from Ideonella sakaiensis 201-F6 (Genbank GAP38373.1)
    Tags
    His
    Expression
    Bacterial
    Mutation
    W159C, S238C; codon optimized for expression in E…
    Promoter
    T7/lac
    Available since
    Feb. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    162675-plasmid-map-sequence-id-321470

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  15. pCJ211

    Plasmid
    #162684
    Purpose
    pET-21b(+) based plasmid for expression of the putative MHETase from Hydrogenophaga sp. PML113 (Genbank WP_083293388.1) with deletion of the predicted signal peptide (Lys2:Gly19), with C-terminal His tag, codon optimized for expression in E. coli K12.
    Depositor
    Gregg Beckham
    Publication
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    Putative MHETase from Hydrogenophaga sp. PML113 (Genbank WP_083293388.1) without 19 residue signal peptide
    Tags
    His
    Expression
    Bacterial
    Mutation
    deltaK2:G19; codon optimized for expression in E.…
    Promoter
    T7/lac
    Available since
    Jan. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    162684-plasmid-map-sequence-id-321526

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  18. pAAV-CaMKII-Cx34.7-M1-2xHA-T2A-mEmerald

    Plasmid
    #255636
    Purpose
    Encodes M. americana Cx34.7, codon optimized for mouse expression with c-terminally fused 2x hemagglutinin (HA) tag, T2A self-cleaving peptide and mEmerald. Mutations E214K and E223K. CaMKII promoter.
    Depositor
    Kafui Dzirasa
    Publication
    Ransey et al Nature. 2026 May 13. doi: 10.1038/s41586
    Insert
    Cx34.7
    Use
    AAV
    Tags
    2xHA-T2A-mEmerald
    Mutation
    E214K,E223K
    Promoter
    CaMKII
    Available since
    June 11, 2026
    Availability
    Academic Institutions and Nonprofits only
    255636-plasmid-map-sequence-id-512735

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  20. pAAV-CaMKII-Cx34.7-M1-mEmerald

    Plasmid
    #255632
    Purpose
    Encodes M. americana Cx34.7, codon optimized for mouse expression, with 8 amino acid linker and c-terminally fused mEmerald tag. Mutations of E214K and E223K. Expression driven by CaMKII promoter.
    Depositor
    Kafui Dzirasa
    Publication
    Ransey et al Nature. 2026 May 13. doi: 10.1038/s41586
    Insert
    Cx34.7
    Use
    AAV
    Tags
    mEmerald
    Mutation
    E214K,E223K
    Promoter
    CaMKII
    Available since
    May 26, 2026
    Availability
    Academic Institutions and Nonprofits only
    255632-plasmid-map-sequence-id-511284

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Showing: 3501 - 3520 of 4953 results