We narrowed to 9,512 results for: try

1. PAK4_HUMAN_D0

   Plasmid

   #79698

   Purpose

   This plasmid encodes the kinase domain of PAK4. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

   Depositor

   Markus Seeliger, Nicholas Levinson, John Chodera

   Article

   Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

   Insert

   PAK4 (PAK4 Human)
   

   Tags

   His10-TEV

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

2. PAK6_HUMAN_D0

   Plasmid

   #79703

   Purpose

   This plasmid encodes the kinase domain of PAK6. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

   Depositor

   Markus Seeliger, Nicholas Levinson, John Chodera

   Article

   Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

   Insert

   PAK6 (PAK6 Human)
   

   Tags

   His10-TEV

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

3. pNC2

   Plasmid

   #72438

   Purpose

   Produces Escherichia coli N5-carboxyaminoimidazole ribonucleotide mutase (EcPurE1); NCAIR synthetase (EcPurK)

   Depositor

   T. J. Kappock, JoAnne Stubbe

   Article

   Meyer et al Biochemistry. 1999 Mar 9;38(10):3012-8.

   Inserts

   N5-carboxyaminoimidazole ribonucleotide mutase
   N5-carboxyaminoimidazole ribonucleotide synthetase
   

   Expression

   Bacterial

   Mutation

   Pro64 to Arg; Asp65 to His; Gln206 to Arg

   Promoter

   T7 and n/a

   Available Since

   May 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJK516

   Plasmid

   #71578

   Purpose

   Produces Acetobacter aceti 1023 succinyl-CoA:acetate CoA-transferase with C-terminal His6 tag and Glu435>Gln mutant (AarCH6-E435Q)

   Depositor

   T. J. Kappock

   Article

   Mullins et al Biochemistry. 2012 Oct 23;51(42):8422-34

   Insert

   succinyl-CoA:acetate CoA-transferase
   

   Tags

   His6

   Expression

   Bacterial

   Mutation

   changes glutamate-435 to glutamine

   Promoter

   T7

   Available Since

   April 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJK524

   Plasmid

   #71580

   Purpose

   Produces Acetobacter aceti 1023 succinyl-CoA:acetate CoA-transferase with C-terminal His6 tag and Asn347>Ala mutant (AarCH6-N347A)

   Depositor

   T. J. Kappock

   Article

   Mullins et al Biochemistry. 2012 Oct 23;51(42):8422-34

   Insert

   succinyl-CoA:acetate CoA-transferase
   

   Tags

   His6

   Expression

   Bacterial

   Mutation

   changes asparagine-347 to alanine

   Promoter

   T7

   Available Since

   April 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJK513

   Plasmid

   #71576

   Purpose

   Produces Acetobacter aceti 1023 succinyl-CoA:acetate CoA-transferase with C-terminal His6 tag and Glu294>Ala mutant (AarCH6-E294A)

   Depositor

   T. J. Kappock

   Article

   Mullins et al Biochemistry. 2012 Oct 23;51(42):8422-34

   Insert

   succinyl-CoA:acetate CoA-transferase
   

   Tags

   His6

   Expression

   Bacterial

   Mutation

   changes glutamate-294 to alanine

   Promoter

   T7

   Available Since

   April 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJK564

   Plasmid

   #71581

   Purpose

   Produces Acetobacter aceti 1023 succinyl-CoA:acetate CoA-transferase with C-terminal His6 tag and Ser71>Ala mutant (AarCH6-S71A)

   Depositor

   T. J. Kappock

   Article

   Mullins et al Biochemistry. 2012 Oct 23;51(42):8422-34

   Insert

   succinyl-CoA:acetate CoA-transferase
   

   Tags

   His6

   Expression

   Bacterial

   Mutation

   changes serine-71 to alanine

   Promoter

   T7

   Available Since

   Jan. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJK517

   Plasmid

   #71579

   Purpose

   Produces Acetobacter aceti 1023 succinyl-CoA:acetate CoA-transferase with C-terminal His6 tag and Glu435>Asp mutant (AarCH6-E435D)

   Depositor

   T. J. Kappock

   Article

   Mullins et al Biochemistry. 2012 Oct 23;51(42):8422-34

   Insert

   succinyl-CoA:acetate CoA-transferase
   

   Tags

   His6

   Expression

   Bacterial

   Mutation

   changes glutamate-435 to aspartate

   Promoter

   T7

   Available Since

   Jan. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJK514

   Plasmid

   #71577

   Purpose

   Produces Acetobacter aceti 1023 succinyl-CoA:acetate CoA-transferase with C-terminal His6 tag and Glu435>Ala mutant (AarCH6-E435A)

   Depositor

   T. J. Kappock

   Article

   Mullins et al Biochemistry. 2012 Oct 23;51(42):8422-34

   Insert

   succinyl-CoA:acetate CoA-transferase
   

   Tags

   His6

   Expression

   Bacterial

   Mutation

   changes glutamine-435 to alanine

   Promoter

   T7

   Available Since

   Jan. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJK512

    Plasmid

    #71575

    Purpose

    Produces Acetobacter aceti 1023 succinyl-CoA:acetate CoA-transferase with C-terminal His6 tag and Arg228>Glu mutant (AarCH6-R228E)

    Depositor

    T. J. Kappock

    Article

    Mullins et al Biochemistry. 2012 Oct 23;51(42):8422-34

    Insert

    succinyl-CoA:acetate CoA-transferase
    

    Tags

    His6

    Expression

    Bacterial

    Mutation

    changes arginine-228 to glutamate

    Promoter

    T7

    Available Since

    Jan. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pFOSGE_COF3H09

    Plasmid

    #71003

    Purpose

    Gateway entry clone

    Depositor

    Alexander Stark

    Article

    Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

    Insert

    SNF4Agamma (SNF4Agamma Fly)
    

    Use

    Gateway entry vector

    Available Since

    Dec. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

12. pFOSGE_COF3C07

    Plasmid

    #70985

    Purpose

    Gateway entry clone

    Depositor

    Alexander Stark

    Article

    Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

    Insert

    14-3-3zeta (14-3-3zeta Fly)
    

    Use

    Gateway entry vector

    Available Since

    Dec. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

13. pFOSGE_COF2B09

    Plasmid

    #70918

    Purpose

    Gateway entry clone

    Depositor

    Alexander Stark

    Article

    Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

    Insert

    gro (gro Fly)
    

    Use

    Gateway entry vector

    Available Since

    Nov. 19, 2015

    Availability

    Academic Institutions and Nonprofits only

14. pFOSGE_COF3C03

    Plasmid

    #70959

    Purpose

    Gateway entry clone

    Depositor

    Alexander Stark

    Article

    Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

    Insert

    Taf4 (Taf4 Fly)
    

    Use

    Gateway entry vector

    Available Since

    Nov. 19, 2015

    Availability

    Academic Institutions and Nonprofits only

15. pDONR221-ARL5B

    Plasmid

    #67326

    Purpose

    hArl5b with stop codon in Gateway Entry vector

    Depositor

    Richard Kahn

    Article

    Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

    Insert

    ARL5B (ARL5B Human)
    

    Use

    Gateway entry vector

    Expression

    Bacterial

    Promoter

    None

    Available Since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

16. pET30Xa-SBL1(160S,NoCys)

    Plasmid

    #66877

    Purpose

    Expresses a cysteine-free version of WTsoybean lipoxygenase-1 160S (4Cys to Ser) with N-term Histag

    Depositor

    Betty Gaffney

    Article

    Coffa et al J Biol Chem. 2005 Nov 18;280(46):38756-6

    Insert

    soybean lipoxygenase-1
    

    Tags

    Histag

    Expression

    Bacterial

    Mutation

    four (all) cysteines mutated to serine

    Promoter

    T7lac

    Available Since

    Aug. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMuLE ENTR SFFV tdTomato L5-L2

    Plasmid

    #62155

    Purpose

    MuLE (Multiple Lentiviral Expression) Entry vector containing a SFFV promoter and tdTomato module. Compatible with MultiSite Gateway cloning

    Depositor

    Ian Frew

    Article

    Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

    Insert

    tdTomato
    

    Use

    Mule gateway entry vector

    Expression

    Mammalian

    Promoter

    SFFV

    Available Since

    June 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMuLE ENTR CMV iRFP L3-L2

    Plasmid

    #62159

    Purpose

    MuLE (Multiple Lentiviral Expression) Entry vector containing a CMV promoter and iRFP module. Compatible with MultiSite Gateway cloning

    Depositor

    Ian Frew

    Article

    Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

    Insert

    iRFP
    

    Use

    Mule gateway entry vector

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMuLE ENTR SV40 PuroR L5-L2

    Plasmid

    #62165

    Purpose

    MuLE (Multiple Lentiviral Expression) Entry vector containing a SV40 promoter and Puromycin module. Compatible with MultiSite Gateway cloning

    Depositor

    Ian Frew

    Article

    Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

    Insert

    Puromycin
    

    Use

    Mule gateway entry vector

    Expression

    Mammalian

    Promoter

    SV40

    Available Since

    June 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMuLE ENTR U6 n.s shRNA L1-L4

    Plasmid

    #62136

    Purpose

    MuLE (Multiple Lentiviral Expression) Entry vector containing a U6 promoter and a non-silencing (scrambled) shRNA module. Compatible with MultiSite Gateway cloning

    Depositor

    Ian Frew

    Article

    Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

    Insert

    non-silencing (scrambled) shRNA
    

    Use

    RNAi; Mule gateway entry vector

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    June 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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