We narrowed to 9,424 results for: try

1. mutpRL-TK 5xUTR

   Plasmid

   #40760

   Purpose

   To evaluate the efficacy of silencing and the extent of cooperativity directed by a small silencing RNA bound at one or multiple sites on an mRNA

   Depositor

   Phillip Zamore

   Article

   Broderick et al RNA. 2011 Oct;17(10):1858-69. Epub 2011

   Insert

   5 copies of the CXCR4 small RNA target site
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Mutation

   mutated from TAG to CTC at nt 387–389 of the Reni…

   Promoter

   TK

   Available since

   Sept. 26, 2013

   Availability

   Academic Institutions and Nonprofits only

2. mutpRL-TK 1xUTR

   Plasmid

   #40756

   Purpose

   To evaluate the efficacy of silencing and the extent of cooperativity directed by a small silencing RNA bound at one or multiple sites on an mRNA

   Depositor

   Phillip Zamore

   Article

   Broderick et al RNA. 2011 Oct;17(10):1858-69. Epub 2011

   Insert

   CXCR4 small RNA target site
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Mutation

   mutated from TAG to CTC at nt 387–389 of the Reni…

   Promoter

   TK

   Available since

   Sept. 26, 2013

   Availability

   Academic Institutions and Nonprofits only

3. CS2-Myc-dLIS1

   Plasmid

   #47047

   Purpose

   Expression of tagged Drosophila LIS1

   Depositor

   Laura Lee

   Article

   Jodoin et al Mol Biol Cell. 2012 Dec;23(24):4713-24.

   Insert

   Lis1 (Lis-1 Fly)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 18, 2013

   Availability

   Academic Institutions and Nonprofits only

4. CS2-CHY-dLIS1

   Plasmid

   #47045

   Purpose

   Expression of fluorescent Drosophila LIS1

   Depositor

   Laura Lee

   Article

   Jodoin et al Mol Biol Cell. 2012 Dec;23(24):4713-24.

   Insert

   Lis1 (Lis-1 Fly)
   

   Use

   Tags

   CHY

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 18, 2013

   Availability

   Academic Institutions and Nonprofits only

5. pDM10

   Plasmid

   #41759

   Depositor

   Jeff Gelles

   Article

   Martin et al Proc Natl Acad Sci U S A. 2010 Mar 23;10

   Insert

   kinesin heavy chain (Khc Fly, E. coli)
   

   Use

   Tags

   His6 and bccp

   Expression

   Bacterial

   Mutation

   residues 1-401; C45S C338S A378C C451S

   Promoter

   Available since

   July 29, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDM8

   Plasmid

   #41758

   Depositor

   Jeff Gelles

   Article

   Martin et al Proc Natl Acad Sci U S A. 2010 Mar 23;10

   Insert

   kinesin heavy chain (Khc Fly, E. coli)
   

   Use

   Tags

   His6 and bccp

   Expression

   Bacterial

   Mutation

   residues 1-401; C45S C338S S364C C451S

   Promoter

   Available since

   July 29, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pOU1

   Plasmid

   #41753

   Depositor

   Jeff Gelles

   Article

   Martin et al Proc Natl Acad Sci U S A. 2010 Mar 23;10

   Insert

   kinesin heavy chain (Khc Fly, E. coli)
   

   Use

   Tags

   His6 and bccp

   Expression

   Bacterial

   Mutation

   residues 1-401; C338S C451S

   Promoter

   Available since

   July 29, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. YopH (residues 164-468)

   Plasmid

   #79749

   Purpose

   This plasmid encodes the active catalytic domain (amino acid residues 164-468) of YopH. Inteded for co-expression with human Tyr kinase plasmids to enhance bacterial kinase expression.

   Depositor

   John Chodera, Nicholas Levinson, Markus Seeliger

   Article

   Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

   Insert

   YopH (residues 164-468)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCF159_BRL

   Plasmid

   #225962

   Purpose

   CMV-Intron-BRL (env protein). Expresses BRL (BaEVRLess) env for VLP production.

   Depositor

   Christof Fellmann

   Article

   Ramadoss et al bioRxiv [Preprint]. 2024 Jun 26:2024.06.

   Insert

   CMV-Intron-BRL (env protein)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. plentiCMV-sct-pp65-b2m-HLA-A0201

    Plasmid

    #196511

    Purpose

    Express single-chain peptide-MHC (pp65 CMV epitope on HLA-A0201)

    Depositor

    Howard Chang

    Article

    Yu et al Cell. 2022 Dec 22;185(26):4904-4920.e22.

    Insert

    single chain of a pp65 peptide, b2m and HLA A0201
    

    Use

    Lentiviral

    Tags

    NA

    Expression

    Mammalian

    Mutation

    Y84C

    Promoter

    CMV

    Available since

    March 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. dCas9-DNMT3a-DNMT3l-3xFLAG-tagBFP

    Plasmid

    #154139

    Purpose

    Expresses the dCas9-DNMT3a-DNMT3l-3xFLAG-tagBFP fusion protein for targeted DNA methylation.

    Depositor

    Albert Jeltsch

    Article

    Hofacker et al Int J Mol Sci. 2020 Jan 13;21(2). pii: i

    Insert

    dCas9, DNMT3a (catalytic domain), DNMT3l (C-terminal part), tagBFP
    

    Use

    Tags

    3x FLAG and tagBFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. mTq2-Calmodulin

    Plasmid

    #198200

    Purpose

    FRET donor: Calmodulin-2 tagged with N-terminal mTurquoise2

    Depositor

    Christian Wahl-Schott

    Article

    Feldmann et al STAR Protoc. 2023 Jul 28;4(3):102459. do

    Insert

    Calmodulin 2 (Calm2 Rat)
    

    Use

    Tags

    mTurquoise2

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. NowGFP /pQE-30

    Plasmid

    #74749

    Purpose

    NowGFP gene for bacterial expression

    Depositor

    Konstantin Lukyanov

    Article

    Sarkisyan et al Biophys J. 2015 Jul 21;109(2):380-9. doi

    Insert

    NowGFP
    

    Use

    Tags

    HisTag (with a mutation but still working)

    Expression

    Bacterial

    Mutation

    WasCFP with substitutions E6K, L42M, T43S, V68M, …

    Promoter

    T5

    Available since

    Sept. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. H6GB1tevP53SS(1-393)

    Plasmid

    #200311

    Purpose

    Bacterial expression of human superstable p53 as His6GB1tev fusion

    Depositor

    Peter Wright

    Article

    Krois et al Biochemistry. 2022 Dec 6;61(23):2709-271

    Insert

    p53 (TP53 Human)
    

    Use

    Tags

    His6-GB1-tev

    Expression

    Bacterial

    Mutation

    M133L, V203A, N239Y, N268D

    Promoter

    T7

    Available since

    April 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. CYFIP1.mCherry

    Plasmid

    #122051

    Purpose

    Expresses human CYFIP1 in mammalian cells.

    Depositor

    Josef Kittler

    Article

    Pathania et al Transl Psychiatry. 2014 Mar 25;4:e374. d

    Insert

    CYFIP1 (CYFIP1 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pENTR1a-tagRFP-KDEL

    Plasmid

    #114177

    Purpose

    Gateway entry clone containing tagRFP with a signal peptide and KDEL ER retention signal for expression of tagRFP in the ER

    Depositor

    Nicolas Demaurex

    Article

    Nunes-Hasler et al Nat Commun. 2017 Nov 24;8(1):1852. doi:

    Insert

    tagRFP-KDEL
    

    Use

    Gateway entry clone

    Tags

    KDEL retention signal and signal peptide

    Expression

    Mutation

    Promoter

    Available since

    Sept. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pIVT-R2Tg-WT

    Plasmid

    #238105

    Purpose

    For IVT of wildtype R2Tg editor, codon optimized for mammalian expression.

    Depositor

    Feng Zhang

    Article

    Edmonds et al Nat Commun. 2025 Jul 2;16(1):6079. doi:

    Insert

    Wildtype R2Tg, mammalian codon optimized
    

    Use

    For ivt

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. HA-MAP1LC3B

    Plasmid

    #137757

    Purpose

    Expression of LC3B

    Depositor

    Carol Mercer

    Article

    Stefely et al Autophagy. 2020 Jan 23. doi: 10.1080/155

    Insert

    MAP1LC3B (MAP1LC3B Human)
    

    Use

    Tags

    HA and myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV FLEX Synaptophysin GFP

    Plasmid

    #137188

    Purpose

    Cre-dependent expression of synaptophysin-GFP

    Depositor

    Matthew Nolan

    Article

    Surmeli et al Neuron. 2016 Nov 23;92(4):929. doi: 10.1

    Insert

    Synaptophysin GFP (Syp Mouse)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pIVT-R2Tg-RTmut

    Plasmid

    #238106

    Purpose

    For IVT of RT-mutant R2Tg editor, codon optimized for mammalian expression.

    Depositor

    Feng Zhang

    Article

    Edmonds et al Nat Commun. 2025 Jul 2;16(1):6079. doi:

    Insert

    Reverse transcriptase mutant R2Tg, mammalian codon optimized
    

    Use

    For ivt

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge