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We narrowed to 40,619 results for: tro

Showing: 7041 - 7080 of 40619 results

  4. AAV-KrasG13V/sgKras/Cre

    Plasmid
    #99860
    Purpose
    Expresses Cre-recombinase, a barcoded HDR template to introduce a G13V mutation into the endogenous Kras locus, and a Kras-targeting sgRNA to enhance HDR.
    Depositor
    Monte Winslow
    Article
    Winters et al Nat Commun. 2017 Dec 12;8(1):2053. doi:
    Insert
    Kras (Kras Mouse)
    Use
    AAV and Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    AvrII site (CAAAGG>CCTAGG), PAM/sgRNA mutation…
    Promoter
    Available since
    Dec. 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    99860-plasmid-map-sequence-id-193770

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  25. GFP Cav2.2 human -HAgHA-minus exon 18a -Short-Cterm - Arg -in pcDNA3

    Plasmid
    #198916
    Purpose
    N-terminal GFP tagged, exofacial HAgHA tag in Domain II, does not contain exon 18a, Short form of alternatively spliced C-teminus, SNP rs2278973 variant Arginine, in pcDNA3 vector
    Depositor
    Annette Dolphin
    Article
    Dahimene et al Function (Oxf). 2023 Oct 19;5(1):zqad060
    Insert
    Cav2.2 (CACNA1B Human)
    Use
    Tags
    N-terminal GFP, internal double HA
    Expression
    Mammalian
    Mutation
    exon 18a deleted, exon 19 starts 3 nucleotides ea…
    Promoter
    CMV
    Available since
    April 1, 2025
    Availability
    Academic Institutions and Nonprofits only
    198916-plasmid-map-sequence-id-392115

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  26. pCMVtrunc msfGFPΔC10-Utr28-222

    Plasmid
    #231558
    Purpose
    Expresses N- and C-terminally truncated Utrophin calponin homology domain fused to C-terminally truncated msfGFP for actin filament organization measurements using fluorescence polarization microscopy
    Depositor
    Manos Mavrakis
    Article
    Martins et al Cell. 2025 Apr 1:S0092-8674(25)00276-4.
    Insert
    Utrophin calponin homology domain (UTRN Human)
    Use
    Tags
    msfGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMVtrunc
    Available since
    Jan. 30, 2025
    Availability
    Academic Institutions and Nonprofits only
    231558-plasmid-map-sequence-id-459698

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  27. pCMV-T7-BPNLS-CE-ABE8e-SpRY--BPNLS-P2A-EGFP (NK289)

    Plasmid
    #208293
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized CE-ABE8e-SpRY A-to-G base editor and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038
    Insert
    pCMV-T7-BPNLS-CE-ABE8e-SpRY-BPNLS-P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS and BPNLS-P2A-EGFP
    Expression
    Mammalian
    Mutation
    ABE8e mutations in TadA inlaid into nSpRY(D10A/A6…
    Promoter
    CMV and T7
    Available since
    Dec. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    208293-plasmid-map-sequence-id-410845

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  28. pCMV-T7-BPNLS-ABE8e(V106W)-SpCas9(D10A)-BPNLS-P2A-EGFP (CA77)

    Plasmid
    #208290
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized ABE8e(V106W) A-to-G base editor with SpCas9(D10A) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038
    Insert
    pCMV-T7-BPNLS-ABE8e(V106W)-SpCas9-BPNLS-P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS and BPNLS-P2A-EGFP
    Expression
    Mammalian
    Mutation
    ABE8e mutations and V106W in TadA and nSpCas9(D10…
    Promoter
    CMV and T7
    Available since
    Dec. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    208290-plasmid-map-sequence-id-410848

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  29. pCMV-T7-BPNLS-ABE8e(V106W)-SpRY-BPNLS-P2A-EGFP (CA136)

    Plasmid
    #208291
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized ABE8e(V106W) A-to-G base editor with SpRY(D10A) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038
    Insert
    pCMV-T7-BPNLS-ABE8e(V106W)-SpRY-BPNLS-P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS and BPNLS-P2A-EGFP
    Expression
    Mammalian
    Mutation
    ABE8e mutations and V106W in TadA and nSpRY(D10A/…
    Promoter
    CMV and T7
    Available since
    Dec. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    208291-plasmid-map-sequence-id-410846

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  30. pBactin-DIO-inv[roxSTOProx-EB3-mNeonGreen-bGH]

    Plasmid
    #196887
    Purpose
    Dual Cre/Dre-dependent expression of EB3-mNeon. Used as functional (labelling of microtubule plus ends) Cre-Dre cotransfection reporter
    Depositor
    Frank Bradke
    Article
    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16
    Insert
    roxSTOProx-EB3 mNeonGreen-bGH (MAPRE3 Human)
    Use
    Cre/Lox; Dre/rox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Chicken bactin (plus Chicken bactin intron)
    Available since
    Aug. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    196887-plasmid-map-sequence-id-442624

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  31. pCMV-T7-ABE8.20m-SpCas9-NRCH-P2A-EGFP (RMD55)

    Plasmid
    #197501
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized ABE(8.20) A-to-G base editor with nSpCas9-NRCH(D10A) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038
    Insert
    pCMV-T7-BPNLS-ABE8.20m-nSpCas9-NRCH-BPNLS-3xFLAG-P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS and BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    ABE8.20 mutations in TadA and nSpCas9-NRCH(D10A/I…
    Promoter
    CMV and T7
    Available since
    March 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    197501-plasmid-map-sequence-id-386690

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  32. pcDNA3.1(+)-Myr-DnaJB1(H33Q)-PKAcat-mCherry

    Plasmid
    #181855
    Purpose
    mCherry-tagged DnaJB1-PKA catalytic subunit fusion N-terminal myristoylation site and H33Q DnaJB1 mutation to block Hsp70 binding; for mammalian expression.
    Depositor
    Jin Zhang
    Article
    Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.
    Insert
    Myr-DnaJB1-PKAcat-mCherry (PRKACA, DNAJB1 Human)
    Use
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Histidine 33 in DnaJB1 changed to glutamine.
    Promoter
    CMV
    Available since
    July 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    181855-plasmid-map-sequence-id-362798

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  33. pcDNA3.1(+)-DnaJB1(H33Q)-PKAcat-mCherry

    Plasmid
    #181854
    Purpose
    mCherry-tagged DnaJB1-PKA catalytic subunit fusion with H33Q DnaJB1 mutation to block Hsp70 binding; for mammalian expression.
    Depositor
    Jin Zhang
    Article
    Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.
    Insert
    DnaJB1-PKAcat-mCherry (PRKACA, DNAJB1 Human)
    Use
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Histidine 33 in DnaJB1 changed to glutamine.
    Promoter
    CMV
    Available since
    July 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    181854-plasmid-map-sequence-id-362794

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  34. pT2iU6Apowriter2N(#355)

    Plasmid
    #184106
    Purpose
    cyclofen-inducible apoptosis activation and imaging (tEosFP) by zebrafish transient transgenesis or mRNA synthesis
    Depositor
    Sophie Vriz
    Article
    Feng et al Rep Prog Phys. 2013 Jul;76(7):072601. do
    Insert
    myr-DIAP1-5myc-tEosFP-nls'-P2A-Casp9-ERT2
    Use
    Zebrafish transient transgenesis + in vitro trans…
    Tags
    internal tag: 5myc
    Expression
    Mutation
    DIAP1: deleted 1-2, N19G, N20V, deleted 146-438; …
    Promoter
    Zf-Ubi+SP6
    Available since
    June 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    184106-plasmid-map-sequence-id-364015

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Showing: 7041 - 7080 of 40619 results