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pN1-HsSmg5-del810-846-V5-SBP-MBP_V

Plasmid

#147765

Purpose

Mammalian Expression of HsSmg5-del810-846

Depositor

Elisa Izaurralde

Article

Loh et al Genes Dev. 2013 Oct 1;27(19):2125-38. do

Insert

HsSmg5-del810-846 (SMG5 Human)

Expression

Mammalian

Mutation

one silent mutation C969T compared to the sequenc…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pN1-HsSmg5-del847-1017-V5-SBP-MBP_V

Plasmid

#147766

Purpose

Mammalian Expression of HsSmg5-del847-1017

Depositor

Elisa Izaurralde

Article

Loh et al Genes Dev. 2013 Oct 1;27(19):2125-38. do

Insert

HsSmg5-del847-1017 (SMG5 Human)

Expression

Mammalian

Mutation

one silent mutation C969T compared to the sequenc…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCIneo-V5-SBP-MBP-HsNanos3_4-20_X

Plasmid

#147923

Purpose

Mammalian Expression of HsNanos3_4-20

Depositor

Elisa Izaurralde

Article

Bhandari et al Genes Dev. 2014 Apr 15;28(8):888-901. do

Insert

HsNanos3_4-20 (NANOS3 Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-MS2-HA-EGFP-HsNanos3_4-20_X

Plasmid

#147930

Purpose

Mammalian Expression of HsNanos3_4-20

Depositor

Elisa Izaurralde

Article

Bhandari et al Genes Dev. 2014 Apr 15;28(8):888-901. do

Insert

HsNanos3_4-20 (NANOS3 Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-MS2-HA-HsSmg5-del133-155-G120E_T

Plasmid

#147554

Purpose

Mammalian Expression of HsSmg5-del133-155-G120E

Depositor

Elisa Izaurralde

Article

Loh et al Genes Dev. 2013 Oct 1;27(19):2125-38. do

Insert

HsSmg5-del133-155-G120E (SMG5 Human)

Expression

Mammalian

Mutation

one silent mutation compared to the sequence give…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-Hsp1-Hsp2Cas9-P2A-puro

Plasmid

#192131

Purpose

Expresses Hsp1-Hsp2Cas9, cloning backbone for sgRNA and puromycin resistance gene

Depositor

Yongming Wang

Article

Gao et al Mol Ther. 2023 Apr 5;31(4):1177-1187. do

Insert

Hsp1-Hsp2Cas9

Use

AAV

Expression

Mammalian

Available Since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pT7-V5-SBP-C1-HsNanos3-Y10E_X

Plasmid

#147966

Purpose

Mammalian Expression of HsNanos3-Y10E

Depositor

Elisa Izaurralde

Article

Bhandari et al Genes Dev. 2014 Apr 15;28(8):888-901. do

Insert

HsNanos3-Y10E (NANOS3 Human)

Expression

Mammalian

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-V5-SBP-C1-HsNanos3-del4-20_X

Plasmid

#147963

Purpose

Mammalian Expression of HsNanos3-del4-20

Depositor

Elisa Izaurralde

Article

Bhandari et al Genes Dev. 2014 Apr 15;28(8):888-901. do

Insert

HsNanos3-del4-20 (NANOS3 Human)

Expression

Mammalian

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-V5-SBP-C1-HsNanos3-F4A_X

Plasmid

#147964

Purpose

Mammalian Expression of HsNanos3-F4A

Depositor

Elisa Izaurralde

Article

Bhandari et al Genes Dev. 2014 Apr 15;28(8):888-901. do

Insert

HsNanos3-F4A (NANOS3 Human)

Expression

Mammalian

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-V5-SBP-C1-HsNanos3-W7E_X

Plasmid

#147965

Purpose

Mammalian Expression of HsNanos3-W7E

Depositor

Elisa Izaurralde

Article

Bhandari et al Genes Dev. 2014 Apr 15;28(8):888-901. do

Insert

HsNanos3-W7E (NANOS3 Human)

Expression

Mammalian

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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KBB345

Plasmid

#185078

Purpose

rfa3D46 truncation fused to GFP

Depositor

Ken Belanger

Article

Belanger et al DNA Cell Biol. 2011 Sep;30(9):641-51. do

Insert

RFA3

Tags

GFP

Expression

Yeast

Mutation

Rfa3 truncation aa 1-46

Available Since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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KBB349

Plasmid

#185080

Purpose

rfa2D248 truncation fused to GFP

Depositor

Ken Belanger

Article

Belanger et al DNA Cell Biol. 2011 Sep;30(9):641-51. do

Insert

RFA2

Tags

GFP

Expression

Yeast

Mutation

Rfa2 truncation aa 1-247

Available Since

July 21, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-MS2-HA-HsNanos3-del4-20_X

Plasmid

#147945

Purpose

Mammalian Expression of HsNanos3-del4-20

Depositor

Elisa Izaurralde

Article

Bhandari et al Genes Dev. 2014 Apr 15;28(8):888-901. do

Insert

HsNanos3-del4-20 (NANOS3 Human)

Expression

Mammalian

Available Since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

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pN1-HsSmg5-G120E-del133-155-V5-SBP-MBP_V

Plasmid

#147767

Purpose

Mammalian Expression of HsSmg5-G120E-del133-155

Depositor

Elisa Izaurralde

Article

Loh et al Genes Dev. 2013 Oct 1;27(19):2125-38. do

Insert

HsSmg5-G120E-del133-155 (SMG5 Human)

Expression

Mammalian

Mutation

one silent mutation C969T compared to the sequenc…

Available Since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

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pETM33_SPSB1_SPRY

Plasmid

#178487

Purpose

Bacterial expression of human domain with His-tag and GST tag

Depositor

Ylva Ivarsson

Article

Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

Insert

SPSB1_SPRY (SPSB1 Human, Synthetic)

Tags

His-GST

Expression

Bacterial

Promoter

T7/LacO

Available Since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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pnEA-NvG-HsNot8_13-240_U

Plasmid

#147649

Purpose

Bacterial Expression of HsNot8_13-240

Depositor

Elisa Izaurralde

Article

Loh et al Genes Dev. 2013 Oct 1;27(19):2125-38. do

Insert

HsNot8_13-240 (CNOT8 Human)

Expression

Bacterial

Available Since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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pTrichoGate-14

Plasmid

#160714

Purpose

For C-terminal tags with Trichoderma optimised Synthetic 3X-c-Myc tag peptide (EQKLISEEDL) bordered by BsaI restriction sites

Depositor

Artemio Mendoza

Article

Nogueira-Lopez et al Front Microbiol. 2019 Dec 10;10:2794. do

Insert

Trichoderma optimised Synthetic 3X-c-Myc tag peptide (EQKLISEEDL)

Use

Synthetic Biology

Available Since

March 8, 2022

Availability

Academic Institutions and Nonprofits only

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pML104-natR412

Plasmid

#162042

Purpose

Expresses Cas9 and contains natR412 guide which targets the natR gene

Depositor

Jolanda Van Leeuwen

Article

Parts et al Mol Syst Biol. 2021 May;17(5):e10138. do

Insert

natR412 guide

Use

CRISPR

Expression

Yeast

Available Since

Feb. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pML107-natR854

Plasmid

#162043

Purpose

Expresses Cas9 and contains natR854 guide which targets the natR gene

Depositor

Jolanda Van Leeuwen

Article

Parts et al Mol Syst Biol. 2021 May;17(5):e10138. do

Insert

natR854 guide

Use

CRISPR

Expression

Yeast

Available Since

Feb. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pET28a-anticalin (E60amb)-His

Plasmid

#168043

Purpose

Expresses anticalin targeting CTLA-4 with the codon encoding E60 changed to amber codon (TAG).

Depositor

Michael Nash

Article

Liu et al Nano Lett. 2022 Jan 12;22(1):179-187. do

Insert

Anticalin

Tags

6x Histag

Expression

Bacterial

Mutation

Glu 60 was mutated to amber codon (TAG)

Promoter

T7 promoter

Available Since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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