We narrowed to 24,603 results for: Nes

1. pLV hUbC-dCas9 VP64-T2A-GFP

   Plasmid

   #53192

   Purpose

   Co-expresses human optimized S. pyogenes dCas9-VP64 and GFP

   Depositor

   Charles Gersbach

   Article

   Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

   Insert

   humanized dead Cas9 VP64 T2A GFP
   

   Use

   CRISPR and Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   D10A and H840A

   Promoter

   hUbC

   Available Since

   June 25, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMC-6-Ruby-9

   Plasmid

   #197740

   Purpose

   Phytobrick (MoClo) Level 0 Part

   Depositor

   Daniel Voytas

   Article

   Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

   Insert

   Ruby reporter tricistronic CDS
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available Since

   May 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET28a_T7-ARG2

   Plasmid

   #106474

   Purpose

   Expresses acoustic reporter genes or gas vesicles (ARG2) in Escherichia coli

   Depositor

   Mikhail Shapiro

   Article

   Bourdeau et al Nature. 2018 Jan 3;553(7686):86-90. doi:

   Insert

   ARG2
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   March 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMaCTag-P21

   Plasmid

   #120032

   Purpose

   Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with 24xSunTag

   Depositor

   Michael Knop

   Article

   Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

   Insert

   24xSunTag
   

   Use

   Pcr template

   Tags

   24xSunTag

   Expression

   Mutation

   Promoter

   None

   Available Since

   Jan. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. 4xHRE_RE:RedF

   Plasmid

   #178314

   Purpose

   HRE RedFirefly luciferase reporter

   Depositor

   Koen Venken

   Article

   Sarrion-Perdigones et al Methods Mol Biol. 2022;2524:409-432. doi

   Insert

   HRE RedFirefly reporter
   

   Use

   Luciferase and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMaCTag-P20

   Plasmid

   #120031

   Purpose

   Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with 10xSuntag

   Depositor

   Michael Knop

   Article

   Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

   Insert

   10xSuntag
   

   Use

   Pcr template

   Tags

   10xSuntag

   Expression

   Mutation

   Promoter

   None

   Available Since

   Jan. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTRANS_231

   Plasmid

   #91120

   Purpose

   Transformation, Type: T-DNA, Plant Selection: 2x35S:bar, Viral Replication: BeYDV

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   35S

   Available Since

   Sept. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLIC-SM-Myc

   Plasmid

   #111189

   Purpose

   High affinity Myc tagging of weakly expressed genes (optimized for Toxoplasma gondii)

   Depositor

   Silvia Moreno

   Article

   Hortua Triana et al J Eukaryot Microbiol. 2018 Apr 19. doi:

   Type

   Empty backbone

   Use

   Toxoplasma gondii expression

   Tags

   Spaghetti Monster Myc Tag

   Expression

   Mutation

   Promoter

   Endogenous

   Available Since

   Aug. 2, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pTnos (GB0037)

   Plasmid

   #68188

   Purpose

   Provides the Agrobacterium tumefaciens Nopaline synthase terminator as a level 0 GoldenBraid part

   Depositor

   Diego Orzaez

   Article

   Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

   Insert

   Nos terminator
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   Available Since

   Oct. 23, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pP35S (GB0030)

    Plasmid

    #68163

    Purpose

    Provides the CaMV 35S Promoter as a level 0 GoldenBraid part

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    CaMV 35S promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available Since

    Oct. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. 560HTR

    Plasmid

    #24444

    Depositor

    Ron Vale

    Article

    Tomishige et al J Cell Biol. 2000 Nov 27. 151(5):1081-92

    Insert

    Kinesin (KIF5B Human)
    

    Use

    Tags

    6XHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    April 23, 2010

    Availability

    Academic Institutions and Nonprofits only

12. UAS:Zebrabow-B

    Plasmid

    #118216

    Purpose

    For broad gal4 inducible expression of Brainbow in zebrafish

    Depositor

    Albert Pan, Alex Schier

    Article

    Pan et al Development. 2013 Jul;140(13):2835-46. d

    Insert

    Brainbow 1.0 L
    

    Use

    Zebrafish expression

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pETM6-H10-mCherry

    Plasmid

    #66531

    Purpose

    Expresses mCherry under the control of Mutant T7 Promoter H10

    Depositor

    Mattheos Koffas

    Article

    Jones et al Sci Rep. 2015 Jun 11;5:11301. doi: 10.10

    Insert

    mCherry
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Codon Optimized for E. coli

    Promoter

    Mutant T7 Promoter - H10

    Available Since

    June 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3/CyclinB1-GFP (28)

    Plasmid

    #39871

    Depositor

    Jonathon Pines

    Article

    Jackman et al Mol Biol Cell. 2002 Mar;13(3):1030-45.

    Insert

    CyclinB1 (CCNB1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    V5A

    Promoter

    Available Since

    June 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. AAV-DeltaFosB

    Plasmid

    #68545

    Purpose

    AAV expression of DeltaFosB

    Depositor

    Eric Nestler

    Article

    Zachariou et al Nat Neurosci. 2006 Feb;9(2):205-11. Epub

    Insert

    DeltaFosB-IRES-GFP
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    truncated splice variant of FosB

    Promoter

    CMV

    Available Since

    July 11, 2018

    Availability

    Academic Institutions and Nonprofits only

16. pmCherry_gRNA

    Plasmid

    #80457

    Purpose

    gRNA expression vector with mCherry transfection control

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

    Inserts

    sp Cas9 gRNA
    mCherry
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and chicken β-actin promoter

    Available Since

    Aug. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMaCTag-Z15

    Plasmid

    #120058

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with miRFP670

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    miRFP670
    

    Use

    Pcr template

    Tags

    miRFP670

    Expression

    Mutation

    Promoter

    None

    Available Since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. PGK-EGFP-FKBPF36V

    Plasmid

    #199765

    Purpose

    plasmid for targeting dTAG into the AAVS1 locus

    Depositor

    Hannes Lans

    Article

    van der Woude et al Nucleic Acids Res. 2023 Oct 13;51(18):e9

    Insert

    GFP-dTAG
    

    Use

    Tags

    GFP and dTAG

    Expression

    Mammalian and Worm

    Mutation

    Promoter

    hPGK

    Available Since

    June 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. AAV-DeltaJunD

    Plasmid

    #68547

    Purpose

    AAV expression of DeltaJunD

    Depositor

    Eric Nestler

    Article

    Winstanley et al J Neurosci. 2007 Sep 26;27(39):10497-507

    Insert

    DeltaJunD-IRES-GFP
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    truncated splice variant

    Promoter

    CMV

    Available Since

    July 11, 2018

    Availability

    Academic Institutions and Nonprofits only

20. pMaCTag-Z08

    Plasmid

    #120051

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with mClover3

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    mClover3
    

    Use

    Pcr template

    Tags

    mClover3

    Expression

    Mutation

    Promoter

    None

    Available Since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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