We narrowed to 7,898 results for: sms

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AAVS1-A2UCOE-EF1a-iCasp9-P2A-HLA-E SCT-T2A-hCD55-E2A-neo-AAVS1

Plasmid

#205444

Purpose

human AAVS1-targeting plasmid for expression of human CD46, HLA-G SCT, CD47, and CD59

Depositor

Deepta Bhattacharya

Article

Pizzato et al Stem Cell Reports. 2023 Dec 22:S2213-671

Insert

iCasp9-HLA-E SCT-hCD55

Expression

Mammalian

Promoter

EF1a

Available Since

Sept. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-eNme2-T.2-ABE8e

Plasmid

#185669

Purpose

Expresses eNme2-T.2-ABE8e in mammalian cells

Depositor

David Liu

Article

Huang et al Nat Biotechnol. 2022 Sep 8. pii: 10.1038

Insert

ecTadA(8e)-neNme2-T.2

Use

CRISPR

Expression

Mammalian

Mutation

Nme2Cas9 D16A/E47K/R63K/V68M/A116T/T123A/D152N/E1…

Available Since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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Proinsulin-NanoLuc in pLX304

Plasmid

#62057

Purpose

Luminescent reporter of insulin secretion

Depositor

David Altshuler

Article

Burns et al Cell Metab. 2015 Jan 6;21(1):126-37. doi

Insert

Proinsulin-NanoLuc (Ins2 Mouse, Synthetic)

Use

Lentiviral and Luciferase

Expression

Mammalian

Mutation

Inserted NanoLuc into C-peptide of mouse Ins2 cod…

Promoter

CMV

Available Since

Jan. 20, 2015

Availability

Academic Institutions and Nonprofits only

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PB713B-pCMV-MITD-NeoMini-T2A-tCD19-WPRE

Plasmid

#174610

Purpose

PiggyBack transposon expressing a fusion of a tandem minigene antigens in KRAS, BRAF and CMV fused to an endosomal targeting sequence and translationally linked to human truncated CD19

Depositor

Stanley Riddell

Article

Veatch et al J Clin Invest. 2021 Aug 16;131(16):e1441

Insert

human CD19, antigen minigene

Expression

Mammalian

Available Since

Oct. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pBacPak-HA-E(y)2

Plasmid

#52288

Purpose

Expresses Drosophila E(y)2

Depositor

Jerry Workman

Article

Mohan et al Genes Dev. 2014 Feb 1;28(3):259-72. doi:

Insert

E(y)2 (e(y)2 Fly)

Tags

HA

Expression

Insect

Available Since

April 24, 2014

Availability

Academic Institutions and Nonprofits only

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pLenti Lifeact-EGFP BlastR

Plasmid

#84383

Purpose

Lentiviral expression of EGFP-tagged Lifeact with Blasticidin selection in cells including neurons

Depositor

Ghassan Mouneimne

Article

Padilla-Rodriguez et al Nat Commun. 2018 Jul 30;9(1):2980. doi:

Insert

Lifeact

Use

Lentiviral

Tags

EGFP

Promoter

CMV immediate early

Available Since

Jan. 5, 2017

Availability

Academic Institutions and Nonprofits only

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opCas12a-T8N (pZZ34, TAT-6MycNLS-opCas12a-2SV40NLS-sfGFP)

Plasmid

#199605

Purpose

Expression of cell permeable opAsCas12a in E. Coli.

Depositor

Junwei Shi

Article

Zhang et al Nat Biotechnol. 2023 Apr 24. doi: 10.103

Insert

opCas12a-T8N

Use

CRISPR

Tags

6xHis, Twin-strep, SUMO

Expression

Bacterial

Available Since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

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CDK2_HUMAN_D0

Plasmid

#79726

Purpose

This plasmid encodes the kinase domain of CDK2. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

CDK2 (CDK2 Human)

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
Cas9-T6N (pZZ12, TAT-4MycNLS-Cas9-2SV40NLS-sfGFP)

Plasmid

#199604

Purpose

Expression of cell permeable spCas9 in E. Coli.

Depositor

Junwei Shi

Article

Zhang et al Nat Biotechnol. 2023 Apr 24. doi: 10.103

Insert

Cas9-T6N

Use

CRISPR

Expression

Bacterial

Available Since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

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pMP71-tCD19-mGFP

Plasmid

#174609

Purpose

expresses membrane-associated (palmitoylated) EGFP and extracellular and transmembrane domains of murine CD19

Depositor

Stanley Riddell

Article

Veatch et al J Clin Invest. 2021 Aug 16;131(16):e1441

Insert

CD19 extracellular and transmembrane domain (Cd19 Mouse)

Tags

palmitoylated EGFP

Expression

Mammalian

Available Since

Oct. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX6p1-GST-Rnf31

Plasmid

#63134

Purpose

Expression of human RNF31/HOIP codon optimized for E. coli and insect cells

Depositor

Titia Sixma

Article

Smit et al EMBO J. 2012 Oct 3;31(19):3833-44. doi:

Insert

Rnf31 (RNF31 Human, Synthetic)

Tags

GST

Expression

Bacterial

Mutation

Synthetic based on human protein sequence

Promoter

tac

Available Since

March 16, 2015

Availability

Academic Institutions and Nonprofits only
pOPINK-OTUB1 (full-length, aa 1-271)

Plasmid

#61420

Purpose

Expresses human OTUB1 (full-length) in E. coli.

Depositor

David Komander

Article

Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

Insert

OTUB1 (Ubiquitin thioesterase OTUB1) (OTUB1 Human)

Tags

His6-GST-3C

Expression

Bacterial

Promoter

T7

Available Since

March 12, 2015

Availability

Academic Institutions and Nonprofits only

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RfxCas13d-backbone

Plasmid

#165078

Purpose

Expression of RfxCas13d sgRNAs from a U6 promoter

Depositor

Ling-Ling Chen

Article

Li et al Nat Methods. 2021 Jan;18(1):51-59. doi:

Insert

Scaffold gRNA

Expression

Mammalian

Available Since

June 16, 2021

Availability

Academic Institutions and Nonprofits only

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WDV-Echistatin

Plasmid

#226985

Purpose

Expresses the HUH endonuclease WDV fused at the C termini with Echistatin to target RGD binding integrins with DNA tension sensors

Depositor

Wendy Gordon

Article

Pawlak et al Nat Commun. 2023 Apr 28;14(1):2468. doi:

Inserts

WDV (repA )
Echistatin

Tags

6xHis and SUMO

Expression

Bacterial

Promoter

T7

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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AAVS1-A2UCOE-EF1a-iCasp9-P2A-Crry-T2A-mCD59-E2A-Qa1 SCT-F2A-neo-AAVS1

Plasmid

#205445

Purpose

human AAVS1-targeting plasmid for expression of iCasp9, Crry, mouse CD59, and Qa1 SCT

Depositor

Deepta Bhattacharya

Article

Pizzato et al Stem Cell Reports. 2023 Dec 22:S2213-671

Insert

iCasp9-Crry-mCD59-Qa1 SCT

Expression

Mammalian

Promoter

EF1a

Available Since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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YFP-UBF1-C1

Plasmid

#134532

Purpose

Expresses YFP-UBF1 fusion protein

Depositor

Susan Janicki

Article

Newhart et al Mol Biol Cell. 2016 Apr 1;27(7):1154-69.

Insert

UBF1 (UBTF Human)

Tags

YFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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Cherry-Fibrillarin-C1

Plasmid

#134539

Purpose

Expresses Cherry-Fibrillarin fusion protein

Depositor

Susan Janicki

Article

Newhart et al Mol Biol Cell. 2016 Apr 1;27(7):1154-69.

Insert

Fibrillarin (FBL Human)

Tags

Cherry

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pLM-mCitrine-Sox2

Plasmid

#23242

Depositor

Michel Sadelain

Article

Papapetrou et al Proc Natl Acad Sci U S A. 2009 Aug 4. 10

Insert

mCitrine_2A_SOX2 (SOX2 Human, synthetic fluorescent protein)

Use

Lentiviral

Available Since

Feb. 5, 2010

Availability

Academic Institutions and Nonprofits only

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pEGFPc1 IP3R3 WT

Plasmid

#236453

Purpose

transient overexpression of IP3R3 in mammalian cells

Depositor

Michele Pagano

Article

Kuchay et al Nature. 2017 Jun 22;546(7659):554-558. d

Insert

IP3R3 (Itpr3 Rat)

Expression

Mammalian

Available Since

May 21, 2025

Availability

Academic Institutions and Nonprofits only

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pMP71-tCD19-llo-mc38tmg

Plasmid

#174603

Purpose

expresses the extracellular and transmembrane domain of murine CD19 with a C terminal fusion of the LLO190 and minigenes of 3 neoantigens from the MC38 tumor in genes ADGPK, DPAGT and Reps1

Depositor

Stanley Riddell

Article

Veatch et al J Clin Invest. 2021 Aug 16;131(16):e1441

Insert

extracell transmem domains moCD19 wCterm fusion LLO190 and minigenes of 3neoantigens MC38tumor in genes ADGPK DPAGT Reps1

Expression

Mammalian

Available Since

Oct. 4, 2021

Availability

Academic Institutions and Nonprofits only

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