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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 6,961 results for: RAP

Showing: 5311 - 5340 of 6961 results

  1. pRRL.SIN.EF1A.JAG1-F1.GS.CAR-2G

    Plasmid
    #194461
    Purpose
    CAR featuring: GMCSF (sig. peptide); CD8A & CD8A (hinge & TM); 4-1BB & CD3ζ (signaling domains); anti-JAG1 from J1.F1 in VH-VL order and (GGGGS)3 linker (scFv). GFP-Zeo for selection and monitoring.
    Depositor
    Ana Sofia Coroadinha
    Article
    Silva et al Biomolecules. 2023 Mar 2;13(3):459. doi:
    Insert
    Anti-JAG1 CAR
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    194461-plasmid-map-sequence-id-384277

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  11. pRRL.SIN.EF1A.JAG1-B5.GS.CAR-2G

    Plasmid
    #194463
    Purpose
    CAR featuring: GMCSF (sig. peptide); CD8A & CD8A (hinge & TM); 4-1BB & CD3ζ (signaling domains); anti-JAG1 from J1.B5 in VH-VL order and (GGGGS)3 linker (scFv). GFP-Zeo for selection and monitoring.
    Depositor
    Ana Sofia Coroadinha
    Article
    Silva et al Biomolecules. 2023 Mar 2;13(3):459. doi:
    Insert
    Anti-JAG1 CAR
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    194463-plasmid-map-sequence-id-384263

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  14. JPF0535a

    Plasmid
    #124042
    Purpose
    Encodes pEF1a driving expression of TET3G P2A iRFP713, pEF1a expressing spdCas9 fused to tagBFP P2A tagBFP, and an sgRNA targeting pTRE expressing mAzamiGreen in a PiggyBac destination vector
    Depositor
    Hana El-Samad
    Article
    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2
    Insert
    PB_pEF1a-tet3G-P2A-iRFP713_pEF1a-spdCAS9::tagBFP-P2A-tagBFP-SV40PA_mu6-sgTRE_pTRE-NLS::mAzamiGreen-rgPA
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    124042-plasmid-map-sequence-id-264228

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  21. pMuLE ENTR SV40-hCas9 L3-L2

    Plasmid
    #62133
    Purpose
    MuLE (Multiple Lentiviral Expression) Entry vector containing SV40 promoter and human codon optimized Cas9 module. Compatible with MultiSite Gateway cloning
    Depositor
    Ian Frew
    Article
    Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d
    Insert
    human codon optimized Cas9
    Use
    CRISPR; Mule gateway entry vector
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    SV40
    Available since
    June 16, 2015
    Availability
    Academic Institutions and Nonprofits only
    62133-plasmid-map-sequence-id-101417

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  25. mKate2-P2A-APEX2-2xML1N(7Q)

    Plasmid
    #67666
    Purpose
    Mammalian expression vector driving APEX2-ML1N (Mouse 2xML1N domain (mutant version), [marks PI(3,5)P2] fused to EM peroxidase marker). Also contains bicistronially expressed cytoplasmic red reporter.
    Depositor
    Rob Parton
    Article
    Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:
    Insert
    mKate2-P2A-APEX2-ML1Nmut*2
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Nov. 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    67666-plasmid-map-sequence-id-117215

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  27. pDP1.2

    Plasmid
    #171092
    Purpose
    Expresses cell cycle-dependent OsTIR1 fused with mEmerald and Geminin (1-110). AKA ROLECCS G2(allows integration into AAVS1 safe-harbor)
    Depositor
    Dario Palmieri
    Article
    Capece et al Cell Death Differ. 2023 Aug 3. doi: 10.1
    Insert
    AAVS1-CMV-OsTIR1-mEmerald-Geminin-Puro-AAVS1
    Use
    Synthetic Biology
    Tags
    Emerald, Geminin
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    171092-plasmid-map-sequence-id-339101

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  28. pLN484 (FuGW-S(cMYC)p-[GAD-Ex1]-[miR1-Mv3 intron]-[GAD-Ex2]-11Pe)

    Plasmid
    #105192
    Purpose
    Module 1 - synthetic promoter cMYC drives self-inhibiting GAD expression (see PMID: 29056342 for detailed information)
    Depositor
    Timothy Lu
    Article
    Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.
    Insert
    S(cMYC)p-[GAD-Ex1]-[miR1-Mv3 intron]-[GAD-Ex2]-11Pe
    Use
    Lentiviral and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 17, 2018
    Availability
    Academic Institutions and Nonprofits only
    105192-plasmid-map-sequence-id-201266

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  30. pMS74 (synthetic guide::∆HYGR::unc-54 3' UTR^SEC)

    Plasmid
    #154838
    Purpose
    Insertion of split hygromycin landing pad into ChrII:8420157 site C. elegans strains via CRISPR with SEC selection
    Depositor
    Patrick Phillips
    Article
    Stevenson et al G3 (Bethesda). 2020 Aug 19. pii: g3.120.
    Insert
    synthetic guide::∆HYGR::unc-54 3' UTR
    Use
    CRISPR and Cre/Lox
    Tags
    Expression
    Worm
    Mutation
    ∆HYGR is promoterless and encodes aa 60-341
    Promoter
    Available since
    Aug. 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    154838-plasmid-map-sequence-id-307904

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Showing: 5311 - 5340 of 6961 results