We narrowed to 497 results for: RAP
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TypeBlog Post... of DNA. 2. The Immortal Rap of Henrietta Lacks Students in Oakland rap about Henrietta Lacks - the...colony collapse disorder. 5. Plasmid Rap Plasmid rap video made with support from Addgene. ...Science rap mastermind, Tom McFadden, recently worked with high school students in the bay area to create...create a plasmid rap video for us (If you’re new to plasmids, we highly recommend checking out the video...video). Tom has made many more Science rap videos to teach students around the globe and is pushing SciComm...Rosalind Franklin vs. Watson & Crick - Science History Rap Battle A great example of science story telling following... science rap...
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AAVs in Retinal Gene Therapy
TypeBlog Post...into the field of gene therapy, more specifically AAV-based retinal gene therapy. The year was 2001 and...for gene (and cell) therapies. One may wonder why the first AAV-based gene therapy treatment for a genetic...forward with ocular gene therapy The pipeline of AAV-based ocular gene therapies is impressive: clinical...candidate therapies for these diseases. Riding on the early successes of retinal gene therapy, an ever-growing...further reading 1. FDA approves gene therapy for blindness 2. Gene Therapy emerges from disgrace to be the ...Stock of Retinal Gene Therapy: Looking Back and Moving Forward. Molecular Therapy Vol. 25 No 5. PubMed ...PMC5960719. 8. Trapani I and Auricchio A., 2018. Seeing the Light after 25 Years of Retinal Gene Therapy. Trends... -
RaPID Detection of RNA-protein Interactions
TypeBlog Post...subtracted from Ramanathan et al.’s RaPID datasets. Applications of RaPID RaPID sounds like a nifty technique...created the RNA-protein interaction detection (RaPID) method. RaPID borrows the E. coli biotin ligase BirA* ...organisms. Detecting RNA-protein interactions with RaPID RaPID is similar to the BioID platform for identifying...signaling. Limitations of RaPID Like other BirA*-based proximity labeling methods, RaPID cannot distinguish ...native regulatory elements. Controls for RaPID experiments RaPID negative controls include both scrambled... of how RaPID can be used to study RNA-protein interactions. Do you have a question that RaPID could help...identifying protein-protein interactions, but RaPID identifies RNA-protein interactions. Both methods rely on... -
Fluorescent Tagging of Endogenous Genes with SapTrap
TypeBlog Post...with SapTrap The Jorgensen Lab recently developed a modular plasmid assembly toolkit called SapTrap to ... improve CRISPR genomic tagging in C. elegans. SapTrap offers scientists a convenient one tube assembly...selection marker for screening modified strains. With SapTrap, the user first designs either oligos or synthetic...site accepts the homology arm repair template. SapTrap includes a prebuilt donor plasmid library containing...insertion. In addition to the obvious advantages SapTrap offers for tagging a single genetic locus, the ... for inserting a tag at multiple target sites. SapTrap vectors can be easily modified to tag hundreds ...degron (AID) tag to generate proteins which can be rapidly and reversibly degraded after the addition of auxin... -
Rett Syndrome: A History of Research and Therapeutic Outlooks
TypeBlog Post...research is now focused on therapeutic strategies that use gene replacement therapy, gene editing, MECP2 reactivation...replacement therapy. In 2014, RSRT brought together a strong collaboration between two gene therapy labs (Brian...Novartis and rebranded to Novartis Gene Therapies, and Taysha Gene Therapies, founded on Gray’s work, are each...and highlights some of the latest breakthrough therapeutic approaches. What is Rett Syndrome? Caused by ...Loss of MECP2 in adult animal models results in rapid symptom onset, suggesting that MECP2 is also essential...syndrome (Tillotson et al., 2017). Advancing gene therapies for Rett Syndrome MECP2 follows the “Goldilocks...Gail Mandel and Stuart Cobb) to create the Gene Therapy Consortium. Leveraging their combined technical... -
Plasmids 101: Gene, enhancer, and promoter trapping
TypeBlog Post...Enhancer traps Like promoter traps, enhancer traps lack enhancer elements and require the trapping of endogenous...circumstances, trap methods may be the preferred screening option. Promoter traps Promoter trap systems lack... enhancer traps! However, don’t count the other traps out just yet. Gene and promoter traps unambiguously... up on their modern counterparts. What are traps? Traps – gene, enhancer, and promoter – all rely on...expression of the gene they monitor. Gene traps Gene traps are promotor-less constructs that contain ...promoter traps. While this system does not require insertion into an exon, like promoter traps, it will...promoter, enhancer, and gene traps at a genomic locus. Choosing a trap At first glance, you may ... -
Adeno Associated Virus (AAV) for Cell and Gene Therapy
TypeBlog Post...translating gene therapies from conceptual design to clinical trials involve identifying a therapeutic gene, finding...effective. Challenges for AAV gene therapy Although AAV-based gene therapies are promising, scientists are ...Howe, and A. J. Thrasher. "Gene therapy progress and prospects: gene therapy for severe combined immunodeficiency...guest blogger Harshana S De Silva Feelixge. Gene therapy technologies hold great promise for improving or...diseases that were previously thought to be incurable. Rapid advances in next generation sequencing technologies...human conditions, opening up new avenues for therapeutics that treat disease at the molecular level. For...immune cell development and function. To date, gene therapy has been used to treat 10 infants with this disease... -
Phage Directory: From Phage Therapy to a Repository of Phage Information
TypeBlog Post...now, phage therapy is not routinely done in most parts of the world. In the US, phage therapy is used as...it comes to administering phage therapy. The laws behind phage therapy, acceptable endotoxin levels, etc..., 2017 Facilitating the search of phages for therapy Phage Directory started out as a directory of phage... only used phage in basic research but not therapeutically: not all labs in Phage Directory have the resources...information and potential for successful phage therapy, Sacher and Zheng also saw the need to archive ... -
Early Career Researcher Toolbox: Free Tools for Making Scientific Graphics
TypeBlog Post...scientific techniques and cool plasmids. Creating graphics were usually the last thing I thought about when...found some free design software that let me create graphics that better communicated the science in my blog...why this was the first program I used to create graphics for the Addgene blog. However, it has a limited...editing shapes and like Vectr, Inkscape uses vector graphics. There’s a steep learning curve for Inkscape, ...for searching the entire internet for scientific graphics. The tools settings let you tailor your search... -
An Interview with Connie Cepko - Gene Therapy, Plasmid Tools and Insights to Success
TypeBlog Post...Eye 9:05-10:16 : Cell Targeting in Retinal Gene Therapies 10:17-14:34 : Progress in Treating Macular Degeneration...Retinopathies 14:39-25:59 : Strategies to Make Gene Therapies More General (more than correcting a defect in...for Delivering Multiple Genes to Cells for Gene Therapy 28:40-35:10 : GFP Activatable Tools Being Developed... gene therapy... -
Ras Pathway
TypeCollection...guanine nucleotide dissociation stimulator RAPGEF RAPGEF1 RAPGEF2 Rap guanine nucleotide exchange factor RAS...protein, LST8 homolog MTOR Mechanistic target of rapamycin NF1 Neurofibromin 1 NFE2L2 Nuclear factor, erythroid...their downstream targets. Rajalingam K, Schreck R, Rapp UR, Albert S. Biochim Biophys Acta. 2007 Aug;1773... . Targeting RAS signalling pathways in cancer therapy. Downward J. Nat Rev Cancer. 2003 Jan;3(1):11-22... -
New Videos: Addgene Lab Tips
TypeBlog Post...Educational Resource: The Addgene Videos Page 5 Science Rap Videos and Their Creator: Tom McFadden Using Video... -
New Educational Resource: The Addgene Videos Page
TypeBlog Post...Additional Resources on the Addgene Blog 5 Science Rap Videos and Their Creator: Tom McFadden SciComm with... -
Oh, The Places You Can Go: Careers in Science Communication - Curriculum Development
TypeBlog Post...out this Addgene podcast with educator and science rap mastermind Tom McFadden Get tips about management... -
How to Lead a Great Meeting
TypeBlog Post...Meetings often get a bad rap as annoying interruptions to our “real” work. However, a well-run meeting... -
Immunology Research Plasmids and Resources
TypeCollection... PILSAP ERAP2 endoplasmic reticulum aminopeptidase 2 FLJ23633, FLJ23701, FLJ23807, L-RAP, LRAP FCER1G ...oncostatin M receptor) CD130, CDw130, GP130, GP130-RAPS, IL6R-beta IL7 interleukin 7 IL-7 IL7R interleukin...RCCP2, c-Met MIA melanoma inhibitory activity CD-RAP MIF macrophage migration inhibitory factor (glycosylation-inhibiting...FGR, YES MGC45350, SLK, SYN GRAP2 GRB2-related adaptor protein 2 GADS, GRAP-2, GRB2L, GRBLG, GRID, GRPL...cells that can recognize a recurrent infection and rapidly initiate a secondary immune response. Browse Plasmids...CATL, CTSL, FLJ31037, MEP CTSS cathepsin S MGC3886 ERAP1 endoplasmic reticulum aminopeptidase 1 A-LAP, ALAP... -
Neurodegeneration Research Collection
TypeCollection...window) An organization with a mission to rapidly develop therapeutics that will slow the progression of Huntington...partly understood, and there are no effective therapies to prevent, treat, or cure most of these disorders...portfolio of biomolecular tools and emphasizes their rapid and broad dissemination to the research community...agenda and to ensuring the development of improved therapies for those living with Parkinson's today. The Michael...available to the scientific community at low cost, with rapid delivery. Includes antibodies, viral vectors, animal... -
CRISPR Plasmids - Tagging
TypeCollection...into human FBL locus Jorgensen Lab SapTrap CRISPR/Cas Toolkit SapTrap is a modular toolkit from Erik Jorgensen's...insert genetic tags in the C. elegans genome. The SapTrap reaction produces a single plasmid targeting vector...26 plasmids; 21 of the plasmids are for use in SapTrap reactions for targeting vector assembly, and the...prebuilt Unc-32::GFP targeting vector. Jorgensen Lab SapTrap CRISPR/Cas Toolkit Kanemaki Lab Auxin-Inducible...cytoplasmic proteins, which can then be depleted very rapidly after the addition of auxin to the culture medium... -
mTOR Pathway
TypeCollection...mTORC2. Each complex contains a unique subunit: raptor for mTORC1, and rictor for mTORC2. Background mTORC1...threonine kinase 11 mTOR Mechanistic target of rapamycin NF1 Neurofibromin 1 PRAS40 Also known as AKT1S1...RRAGA RRAGB RRAGC RRAGD Ras-related GTP binding Raptor Also known as RPTOR; Regulatory associated protein...associated protein 1 mTOR Mechanistic target of rapamycin p53 TP53; tumor protein p53 PDK1 Pyruvate dehydrogenase...10.1016/j.cell.2012.03.017. PubMed PMID: 22500797 . Rapamycin: one drug, many effects. Li J, Kim SG, Blenis ... -
Worm Expression Resources
TypeCollection...widely used as a genetic model organism due to their rapid life cycle (3 days from egg to adult) and easy lab...along with links to protocols, can be found below: SapTrap-SEC kit for C. elegans genome engineering - Bob...kit can be found at the Goldstein Lab website . SapTrap CRISPR/Cas Toolkit - Erik Jorgensen Lab. A collection...Efficiency Fluorescent Tagging of Endogenous Genes with SapTrap Even more elegant: Single injection CRISPR/Cas9...