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We narrowed to 419 results for: RAP

Showing: 1 - 20 of 419 results

  1. 5 Science Rap Videos & Their Creator: Tom McFadden

    Type
    Blog Post
    Published
    Feb. 9, 2017, 3:30 p.m.
    ... of DNA. 2. The Immortal Rap of Henrietta Lacks Students in Oakland rap about Henrietta Lacks - the...colony collapse disorder.     5. Plasmid Rap Plasmid rap video made with support from Addgene.   ...Science rap mastermind, Tom McFadden, recently worked with high school students in the bay area to create...create a plasmid rap video for us (If you’re new to plasmids, we highly recommend checking out the video...video). Tom has made many more Science rap videos to teach students around the globe and is pushing SciComm...Rosalind Franklin vs. Watson & Crick - Science History Rap Battle A great example of science story telling following... science rap...

  2. AAVs in Retinal Gene Therapy

    Type
    Blog Post
    Published
    July 31, 2018, 12:55 p.m.
    ...into the field of gene therapy, more specifically AAV-based retinal gene therapy. The year was 2001 and...for gene (and cell) therapies. One may wonder why the first AAV-based gene therapy treatment for a genetic...forward with ocular gene therapy The pipeline of AAV-based ocular gene therapies is impressive: clinical...candidate therapies for these diseases. Riding on the early successes of retinal gene therapy, an ever-growing...further reading 1. FDA approves gene therapy for blindness 2. Gene Therapy emerges from disgrace to be the ...Stock of Retinal Gene Therapy: Looking Back and Moving Forward. Molecular Therapy Vol. 25 No 5. PubMed ...PMC5960719. 8. Trapani I and Auricchio A., 2018. Seeing the Light after 25 Years of Retinal Gene Therapy. Trends...

  3. RaPID Detection of RNA-protein Interactions

    Type
    Blog Post
    Published
    Aug. 16, 2018, 12:40 p.m.
    ...subtracted from Ramanathan et al.’s RaPID datasets. Applications of RaPID RaPID sounds like a nifty technique...created the RNA-protein interaction detection (RaPID) method. RaPID borrows the E. coli biotin ligase BirA* ...organisms. Detecting RNA-protein interactions with RaPID RaPID is similar to the BioID platform for identifying...signaling. Limitations of RaPID Like other BirA*-based proximity labeling methods, RaPID cannot distinguish ...native regulatory elements. Controls for RaPID experiments RaPID negative controls include both scrambled... of how RaPID can be used to study RNA-protein interactions. Do you have a question that RaPID could help...identifying protein-protein interactions, but RaPID identifies RNA-protein interactions. Both methods rely on...

  4. Fluorescent Tagging of Endogenous Genes with SapTrap

    Type
    Blog Post
    Published
    Oct. 12, 2017, 2:26 p.m.
    ...with SapTrap The Jorgensen Lab recently developed a modular plasmid assembly toolkit called SapTrap to ... improve CRISPR genomic tagging in C. elegans. SapTrap offers scientists a convenient one tube assembly...selection marker for screening modified strains. With SapTrap, the user first designs either oligos or synthetic...site accepts the homology arm repair template. SapTrap includes a prebuilt donor plasmid library containing...insertion.   In addition to the obvious advantages SapTrap offers for tagging a single genetic locus, the ... for inserting a tag at multiple target sites. SapTrap vectors can be easily modified to tag hundreds ...degron (AID) tag to generate proteins which can be rapidly and reversibly degraded after the addition of auxin...

  5. Rett Syndrome: A History of Research and Therapeutic Outlooks

    Type
    Blog Post
    Published
    Nov. 19, 2020, 2:15 p.m.
    ...research is now focused on therapeutic strategies that use gene replacement therapy, gene editing, MECP2 reactivation...replacement therapy. In 2014, RSRT brought together a strong collaboration between two gene therapy labs (Brian...Novartis and rebranded to Novartis Gene Therapies, and Taysha Gene Therapies, founded on Gray’s work, are each...and highlights some of the latest breakthrough therapeutic approaches. What is Rett Syndrome? Caused by ...Loss of MECP2 in adult animal models results in rapid symptom onset, suggesting that MECP2 is also essential...syndrome (Tillotson et al., 2017). Advancing gene therapies for Rett Syndrome MECP2 follows the “Goldilocks...Gail Mandel and Stuart Cobb) to create the Gene Therapy Consortium. Leveraging their combined technical...

  6. Plasmids 101: Gene, enhancer, and promoter trapping

    Type
    Blog Post
    Published
    May 2, 2023, 1 p.m.
    ...Enhancer traps Like promoter traps, enhancer traps lack enhancer elements and require the trapping of endogenous...circumstances, trap methods may be the preferred screening option. Promoter traps Promoter trap systems lack... enhancer traps! However, don’t count the other traps out just yet. Gene and promoter traps unambiguously... up on their modern counterparts. What are traps? Traps – gene, enhancer, and promoter – all rely on...expression of the gene they monitor. Gene traps Gene traps are promotor-less constructs that contain ...promoter traps. While this system does not require insertion into an exon, like promoter traps, it will...promoter, enhancer, and gene traps at a genomic locus.    Choosing a trap At first glance, you may ...

  7. Adeno Associated Virus (AAV) for Cell and Gene Therapy

    Type
    Blog Post
    Published
    Nov. 7, 2017, 1:59 p.m.
    ...translating gene therapies from conceptual design to clinical trials involve identifying a therapeutic gene, finding...effective. Challenges for AAV gene therapy Although AAV-based gene therapies are promising, scientists are ...Howe, and A. J. Thrasher. "Gene therapy progress and prospects: gene therapy for severe combined immunodeficiency...guest blogger Harshana S De Silva Feelixge. Gene therapy technologies hold great promise for improving or...diseases that were previously thought to be incurable. Rapid advances in next generation sequencing technologies...human conditions, opening up new avenues for therapeutics that treat disease at the molecular level. For...immune cell development and function. To date, gene therapy has been used to treat 10 infants with this disease...

  8. Phage Directory: From Phage Therapy to a Repository of Phage Information

    Type
    Blog Post
    Published
    Jan. 10, 2019, 2:38 p.m.
    ...now, phage therapy is not routinely done in most parts of the world. In the US, phage therapy is used as...it comes to administering phage therapy. The laws behind phage therapy, acceptable endotoxin levels, etc..., 2017 Facilitating the search of phages for therapy Phage Directory started out as a directory of phage... only used phage in basic research but not therapeutically: not all labs in Phage Directory have the resources...information and potential for successful phage therapy, Sacher and Zheng also saw the need to archive ...

  9. Early Career Researcher Toolbox: Free Tools for Making Scientific Graphics

    Type
    Blog Post
    Published
    June 11, 2019, 1:16 p.m.
    ...scientific techniques and cool plasmids. Creating graphics were usually the last thing I thought about when...found some free design software that let me create graphics that better communicated the science in my blog...why this was the first program I used to create graphics for the Addgene blog. However, it has a limited...editing shapes and like Vectr, Inkscape uses vector graphics. There’s a steep learning curve for Inkscape, ...for searching the entire internet for scientific graphics. The tools settings let you tailor your search...

  10. An Interview with Connie Cepko - Gene Therapy, Plasmid Tools and Insights to Success

    Type
    Blog Post
    Published
    Dec. 8, 2015, 3:30 p.m.
    ...Eye 9:05-10:16 : Cell Targeting in Retinal Gene Therapies 10:17-14:34 : Progress in Treating Macular Degeneration...Retinopathies 14:39-25:59 : Strategies to Make Gene Therapies More General (more than correcting a defect in...for Delivering Multiple Genes to Cells for Gene Therapy 28:40-35:10 : GFP Activatable Tools Being Developed... gene therapy...

  11. New Videos: Addgene Lab Tips

    Type
    Blog Post
    Published
    Feb. 15, 2018, 2:41 p.m.
    ...Educational Resource: The Addgene Videos Page 5 Science Rap Videos and Their Creator: Tom McFadden Using Video...

  14. How to Lead a Great Meeting

    Type
    Blog Post
    Published
    Feb. 9, 2016, 3:30 p.m.
    ...Meetings often get a bad rap as annoying interruptions to our “real” work. However, a well-run meeting...

  15. Cancer and the Immune System: Deciphering the Relationship

    Type
    Blog Post
    Published
    March 14, 2017, 2:30 p.m.
    ...Existing therapies and effects on immune system Conventional cancer therapies include radiation therapy (owing...Curie), chemotherapy, and surgical removal of the tumor. However, these conventional therapies produce... Types of immunotherapeutics In lieu of the cancer-immune system interactions, therapeutics have been ...beneficiaries. Collaborative therapy For the most efficient outcomes, collaborative therapies must be explored. ...probability of a relapse. Chemical and radiation therapies cause a transient shutdown of the immune system...boost the activity of the immune system, the therapeutic ability of even these conventional antitumor ...proteins are one of the most commonly used immunotherapeutic agents. Currently the FDA has 12 approved ...

  16. CRISPR in the Clinic

    Type
    Blog Post
    Published
    Nov. 2, 2023, 1:15 p.m.
    ...Current CRISPR Therapies Cancer and CAR-T therapy The FDA has already approved CAR-T immunotherapy for cancer...CRISPR therapies CRISPR technology is rapidly evolving, and many of the challenges with therapies involve...delivery and editing strategies for CRISPR therapies. Systemic therapies involve CRISPR-Cas delivery directly... initiated a CRISPR therapy for hATTR with the first-ever systemic CRISPR therapy. They engineered a CRISPR-Cas...this therapy is affecting hATTR patient outcomes. Bacterial infection and HIV Most CRISPR therapies have...sponsored by Excision Biotherapeutics. Additional ongoing trials The CRISPR therapies don’t stop with the...longer an accurate descriptor; progress in clinical therapy is more fitting, so it is time to update our post...

  17. Mesothelioma - Causes, Symptoms, and Treatment

    Type
    Blog Post
    Published
    Dec. 13, 2016, 3:30 p.m.
    ...treatments, such as surgery, radiation, and chemotherapy, immunotherapy is coming to the fore as a cutting edge...form of treatment known as hyperthermic intraperitoneal chemotherapy (HIPEC). With this technique, surgery...performs a heated chemotherapy wash throughout the abdominal cavity. This targeted chemotherapy approach has...susceptible to up and coming immunotherapies. The standard definition of immunotherapy is the use of drugs (or...research opportunities are being provided to immunotherapy than ever before. Although different types of...great promise in up and coming treatments like immunotherapy. Mesothelioma Mesothelioma is a terrible disease...cancerous masses (tumor resection), systemic chemotherapy to kill cancerous cells that may have spread...

  18. Meet the New Addgene Board Members!

    Type
    Blog Post
    Published
    April 28, 2020, 1:14 p.m.
    ...employees at Dimension Therapeutics, she helped build and grow the gene therapy division of the company...journal Molecular Therapy. Vandenberge is also the Grousbeck Family Chair in Gene Therapy, Director of the...Khandan Baradaran’s expertise in viral safety, gene therapy vector development, vaccine development, and assay...Addgene. She was Head of Quality at Dimension Therapeutics, which was acquired by Ultragenyx Pharmaceutical...Stegmeier is the Chief Scientific Officer at KSQ Therapeutics and is an Addgene depositor. KSQ uses a CRISPR-Cas9...crises like those and ended up in the field of gene therapy.” He’s also worked on vaccines throughout that ...a variety of commercialization efforts in gene therapy either through direct industry collaborations, ...

  19. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

    Type
    Blog Post
    Published
    May 10, 2016, 2:30 p.m.
    ...challenge for CRISPR HIV-1 therapies is sequence specificity. Whereas antiviral therapies target HIV-1 at the...Today, HIV-1 can be controlled with antiviral therapies such that the virus is undetectable in the blood...demonstrated two potential strategies for CRISPR-HIV therapeutics - one using dCas9-SAM to activate HIV-1 transcription...untreated individuals. Symptoms of AIDS include rapid weight loss and an increased risk of infection, ...usually seen in healthy individuals. Antiretroviral therapy (ART) can virtually eliminate plasma HIV-1, improving...the probability of off-target effects. In such a therapy, CRISPR/Cas9 would need to 1. excise the HIV-1 ...most HIV-1 patients. Obstacles for CRISPR HIV-1 therapy Both of these methods represent exciting advances...

  20. Viral Vectors 101: AAV Serotypes and Tissue Tropism

    Type
    Blog Post
    Published
    Jan. 16, 2025, 3:42 p.m.
    ...human gene therapy. Molecular Therapy: The Journal of the American Society of Gene Therapy, 14(3), 316...AAV1 was the first viral vector approved for gene therapy (Issa et al., 2023). It may be a good choice for...high transduction efficiency in multiple tissues, rapid transgene expression, and can cross blood vessel...rAAV treatment, which can pose problems for gene therapy applications. Cross-reactivity of AAV serotypes...AAV7, and AAV8 in the Mouse and Rat. Human Gene Therapy, 19(12), 1359–1368. https://doi.org/10.1089/hum...evolution of a new adeno-associated virus for therapeutic outer retinal gene delivery from the vitreous...underexplored sites on the AAV capsid. Molecular Therapy. Methods & Clinical Development, 26, 331–342. https...
Showing: 1 - 20 of 419 results