We narrowed to 20 results for: RAP

...Fisher 21028 Protein G GraviTrap columns, Cytiva 28-9852-55 or rProtein A GraviTrap columns, Cytiva 28-9852...and mix well. Uncap the Protein G Gravitrap or rProtein A Gravitrap columns and gently pour off the ethanol...Buffer Reservoirs to the top of the Gravitrap column. Pro-Tip Gravitrap columns have a finite binding capacity...the Gravitrap column to a clamp on a clamp stand. Use scissors to cut the bottom of the Gravitrap column...the Gravitrap columns at the bottom. Add 5 mL of Pierce IgG Elution Buffer to the capped Gravitrap column...430281 0.45 µm PES complete filtration unit, 500 mL, rapid-flow, VWR 73520-984 Syringes, 30 mL, VWR BD302832...upright at -20 °C Procedure Section 1: Affinity chromatography Equilibrate filtered tissue culture supernatant...

...during the scraping process. Add each scrape into a 50 mL conical tube on ice pooling the scrapings of two...spreader and cold LB to scrape bioassay plates and remove bacteria. Use one scraper for all plates. Use two...mL cold LB to each plate for each scrape and use spreader to scrape plates. Pro-Tip Pushing motion is ... each tube on ice while scraping. Repeat addition of 10 mL cold LB and scrape for each plate until agar...spreading. Some spreaders have a sharp edge that can scrape plates more abrasively at a certain angle. Incubate...conical tubes on ice and ensure access to sterile scraper and cold LB. Pro-Tip Prepare one to two more conical...removing LB containing bacteria from plate). Pro-Tip Scraped bacteria in LB can clog the pipette either mix ...

... to collect the next fraction. The flow will be rapid at first and will progressively slow down. Option...methods improves infectious titer and yield." Gene therapy 6.6 (1999): 973-985 (Link opens in a new window... Rist Wolfgang, and Lamla Thorsten. Human Gene Therapy Methods. August 2015, 26(4): 147-157. doi:10.1089...

...microcentrifuge. Remove the gel from the plastic wrapper and rinse with deionized water. Gently remove the...Analyze . Select Gels . Select Plot Lanes . One graph per lane will appear with peaks representing each...

...microtiter plate Chemiluminescence substrate Plastic wrap Primary antibody Secondary antibody Deionized water...BSA standard concentration versus absorbance. Extrapolate the total protein concentration of the sample...

...565-0010 (or for larger volumes 1000 mL 0.45 µm Rapid-Flow PES Filtration Unit, Nalgene 167-0045) Pro-...-Tip The pH of this solution will drift pretty rapidly upon addition of acid or base. Add only a few drops...

...use a sterile loop, toothpick or pipette tip to scrape some of the frozen bacteria off of the top. Do ...

...after Over-Agar Plating of Carbenicillin. The above graph displays the stock concentration of Carbenicillin...

...sequencing results as a trace file (.ab1) which graphically depicts the sequence as a series of colored peaks...

...associated with freeze-thaw. If using frozen virus, rapidly thaw the lentiviral aliquot on ice prior to use...

...-Tip The pH of this solution will drift pretty rapidly upon addition of acid or base. Add only a few drops...

...min at RT in the dark. Pro-Tip The plate can be wrapped in foil to block light. Remove the secondary antibody...

...first. Pro-Tip The pH of this solution will drift rapidly upon addition of acid or base. Add only a few drops...

...have dissolved. Note: The powder should dissolve rapidly (with 20 min) but check for the presence of particles...

...solution has a tendancy to boil over. Placing saran wrap over the top of the flask can help with this, but...

...elongate the new target DNA strand accurately and rapidly. Repeat steps 2-4 25-30 times. Final Extension ...

...especially if that transgene is toxic. Eventually, the rapidly growing low-level transgene expressors may take...

...analyzing the gene’s function. The process is shown graphically in the following cartoon, in which we are adding...

...each well. Cover the plate with a plate seal and wrap in foil. Incubate plate at room temperature on a...

...accuracy of its literature, but realizes that typographical or other errors may occur. Addgene makes no ...