We narrowed to 3,807 results for: golden gate

1. pMBS-869

   Plasmid

   #218021

   Purpose

   ER retention signal (DGDL) for protein targeting to ER

   Depositor

   Michael Schroda

   Article

   Hieronimus et al Microb Cell. 2024 Apr 30;11:128-142. doi

   Insert

   ER retention signal (DGDL)
   

   Use

   Synthetic Biology

   Available Since

   Aug. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCM0-101

   Plasmid

   #218199

   Purpose

   Multi-Stop for adding 3 stops for each frame

   Depositor

   Michael Schroda

   Article

   Hieronimus et al Microb Cell. 2024 Apr 30;11:128-142. doi

   Insert

   Multi-Stop
   

   Use

   Synthetic Biology

   Available Since

   Aug. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCM0-099

   Plasmid

   #218201

   Purpose

   Strep-8xHis-tag for protein detection and purification

   Depositor

   Michael Schroda

   Article

   Hieronimus et al Microb Cell. 2024 Apr 30;11:128-142. doi

   Insert

   Strep-8xHis
   

   Use

   Synthetic Biology

   Available Since

   Aug. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCM0-100

   Plasmid

   #218202

   Purpose

   3xHA epitope tag for protein detection

   Depositor

   Michael Schroda

   Article

   Hieronimus et al Microb Cell. 2024 Apr 30;11:128-142. doi

   Insert

   3xHA
   

   Use

   Synthetic Biology

   Available Since

   Aug. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pMBS-723

   Plasmid

   #218203

   Purpose

   1xHA-8xHis-tag for protein detection and purification

   Depositor

   Michael Schroda

   Article

   Hieronimus et al Microb Cell. 2024 Apr 30;11:128-142. doi

   Insert

   1xHA-8xHis
   

   Use

   Synthetic Biology

   Available Since

   Aug. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCM0-054

   Plasmid

   #218204

   Purpose

   SV40 NLS for protein targeting to nucleus

   Depositor

   Michael Schroda

   Article

   Hieronimus et al Microb Cell. 2024 Apr 30;11:128-142. doi

   Insert

   SV40 NLS
   

   Use

   Synthetic Biology

   Available Since

   Aug. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMBS-838

   Plasmid

   #217994

   Purpose

   pLEXSY_I-blecherry3_dom_lacZ Destination Vector

   Depositor

   Michael Schroda

   Article

   Hieronimus et al Microb Cell. 2024 Apr 30;11:128-142. doi

   Type

   Empty backbone

   Use

   Synthetic Biology

   Promoter

   T7 promotor

   Available Since

   Aug. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMBS-840

   Plasmid

   #217996

   Purpose

   3xHA epitope tag for protein detection

   Depositor

   Michael Schroda

   Article

   Hieronimus et al Microb Cell. 2024 Apr 30;11:128-142. doi

   Insert

   3xHA
   

   Use

   Synthetic Biology

   Available Since

   Aug. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pMBS-843

   Plasmid

   #217997

   Purpose

   smtHSP60 for protein targeting to mitochondrial matrix

   Depositor

   Michael Schroda

   Article

   Hieronimus et al Microb Cell. 2024 Apr 30;11:128-142. doi

   Insert

   smtHSP60
   

   Use

   Synthetic Biology

   Available Since

   Aug. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pMBS-844

    Plasmid

    #217998

    Purpose

    Nucleoplasmin NLS for protein targeting to nucleus

    Depositor

    Michael Schroda

    Article

    Hieronimus et al Microb Cell. 2024 Apr 30;11:128-142. doi

    Insert

    Nucleoplasmin NLS
    

    Use

    Synthetic Biology

    Available Since

    Aug. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pMBS-1125

    Plasmid

    #217999

    Purpose

    Start-methionine

    Depositor

    Michael Schroda

    Article

    Hieronimus et al Microb Cell. 2024 Apr 30;11:128-142. doi

    Insert

    Start-Met
    

    Use

    Synthetic Biology

    Available Since

    Aug. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pMBS-846

    Plasmid

    #218001

    Purpose

    3xFLAG-tag fused to PreScission Protease cleavage site for protein purification and cleavage

    Depositor

    Michael Schroda

    Article

    Hieronimus et al Microb Cell. 2024 Apr 30;11:128-142. doi

    Insert

    3xFLAG-tag
    

    Use

    Synthetic Biology

    Tags

    PreScission protease cleavage site

    Available Since

    Aug. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMBS-847

    Plasmid

    #218002

    Purpose

    Myc-tag fused to PreScission Protease cleavage site for protein purification and cleavage

    Depositor

    Michael Schroda

    Article

    Hieronimus et al Microb Cell. 2024 Apr 30;11:128-142. doi

    Insert

    Myc-tag
    

    Use

    Synthetic Biology

    Tags

    PreScission protease cleavage site

    Available Since

    Aug. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMBS-851

    Plasmid

    #218006

    Purpose

    Spacer to bridge position B3

    Depositor

    Michael Schroda

    Article

    Hieronimus et al Microb Cell. 2024 Apr 30;11:128-142. doi

    Insert

    Spacer
    

    Use

    Synthetic Biology

    Available Since

    Aug. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pECNUS2

    Plasmid

    #184885

    Purpose

    ABE cloning vector, insertion of gRNA sequence via Golden Gate with BsaI and T4 ligase, blue-white screening. Replicates in E.coli and Agrobacterium, binary vector for Agrobacterium T-DNA delivery

    Depositor

    Eunyoung Chae

    Article

    Tan et al Mol Plant Microbe Interact. 2025 Feb;38(

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Plant

    Promoter

    RPS5A

    Available Since

    Aug. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pECNUS3

    Plasmid

    #184886

    Purpose

    ABE cloning vector, insertion of gRNA sequence via Golden Gate with BsaI and T4 ligase, blue-white screening. Replicates in E.coli and Agrobacterium, binary vector for Agrobacterium T-DNA delivery

    Depositor

    Eunyoung Chae

    Article

    Tan et al Mol Plant Microbe Interact. 2025 Feb;38(

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Plant

    Promoter

    RPS5A

    Available Since

    July 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pECNUS1

    Plasmid

    #184884

    Purpose

    ABE cloning vector, insertion of gRNA sequence via Golden Gate with BsaI and T4 ligase, blue-white screening. Replicates in E.coli and Agrobacterium, binary vector for Agrobacterium T-DNA delivery

    Depositor

    Eunyoung Chae

    Article

    Tan et al Mol Plant Microbe Interact. 2025 Feb;38(

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Plant

    Promoter

    RPS5A

    Available Since

    July 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLV-EF1a-NLS-msfGFP-T2A-mCherry-NES> mPGK-PuroR

    Plasmid

    #218916

    Purpose

    Lentiviral construct expressing msfGFP and nuclear mCherry proteins.

    Depositor

    Sven Eyckerman

    Article

    Delhaye et al Cell Rep Methods. 2024 Jul 15;4(7):10081

    Insert

    EF1a-msfGFP-T2A-mCherry-NLS > mPGK-PuroR
    

    Use

    Lentiviral

    Available Since

    June 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pUC57-mini v2-HF24_F4

    Plasmid

    #195718

    Purpose

    This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.

    Depositor

    Eric Cantor

    Article

    Potapov et al ACS Synth Biol. 2018 Nov 16;7(11):2665-2

    Insert

    Fragment#4 of 24 fragment splits in lacI/lacZ cassette
    

    Use

    Synthetic Biology

    Available Since

    March 20, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. pUC57-mini v2-HF24_F20

    Plasmid

    #195734

    Purpose

    This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.

    Depositor

    Eric Cantor

    Article

    Potapov et al ACS Synth Biol. 2018 Nov 16;7(11):2665-2

    Insert

    Fragment#20 of 24 fragment splits in lacI/lacZ cassette
    

    Use

    Synthetic Biology

    Available Since

    March 16, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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