We narrowed to 6,963 results for: RAP

1. ZIKV_NASBA_32B

   Plasmid

   #75011

   Purpose

   Portion of Zika Virus genome (KU312312: 7066-7399) containing sensor 32B trigger sequence and NASBA amplification sites

   Depositor

   Alexander Green, James Collins

   Article

   Pardee et al Cell. 2016 May 6. pii: S0092-8674(16)305

   Insert

   ZIKV_NASBA_32B
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMS74 (synthetic guide::∆HYGR::unc-54 3' UTR^SEC)

   Plasmid

   #154838

   Purpose

   Insertion of split hygromycin landing pad into ChrII:8420157 site C. elegans strains via CRISPR with SEC selection

   Depositor

   Patrick Phillips

   Article

   Stevenson et al G3 (Bethesda). 2020 Aug 19. pii: g3.120.

   Insert

   synthetic guide::∆HYGR::unc-54 3' UTR
   

   Use

   CRISPR and Cre/Lox

   Tags

   Expression

   Worm

   Mutation

   ∆HYGR is promoterless and encodes aa 60-341

   Promoter

   Available since

   Aug. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. ZIKV_NASBA_27B

   Plasmid

   #75010

   Purpose

   Portion of Zika Virus genome (KU312312: 1248-1594) containing sensor 27B trigger sequence and NASBA amplification sites

   Depositor

   Alexander Green, James Collins

   Article

   Pardee et al Cell. 2016 May 6. pii: S0092-8674(16)305

   Insert

   ZIKV_NASBA_27B
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. PL752-EN1-LA-p2a-mTagBFP2nls-3xHAtag-hGHpA-PGK-puroR-EN1-RA

   Plasmid

   #191691

   Purpose

   For knocking p2a-mTagBFP2nls-3xHAtag into EN1 locus

   Depositor

   Yuejun Chen

   Article

   Xu et al J Clin Invest. 2022 Jul 15;132(14). pii:

   Insert

   EN1 homology arms and p2a-mTagBFP2nls-3xHAtag
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   Available since

   Nov. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pQC hKGA.wt V5-His IRES G418

   Plasmid

   #110390

   Purpose

   γ-Retroviral transfer vector for expressing glutminase isoform KGA (wild type), C-terminal V5-6xHis tags, IRES-driven Geneticin selection.

   Depositor

   Sandra Martha Gomes Dias

   Insert

   KGA glutaminase (GLS Human)
   

   Use

   Retroviral

   Tags

   6xHis and V5

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pGL-p21UTRm2

   Plasmid

   #20879

   Depositor

   Robert Blelloch

   Article

   Wang et al Nat Genet. 2008 Dec . 40(12):1478-83.

   Insert

   p21 3'UTR site 2 mutation (Cdkn1a Mouse)
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Predicted miRNA binding site 2 (Position ~1100) G…

   Promoter

   Available since

   March 12, 2012

   Availability

   Academic Institutions and Nonprofits only

7. pGEMT-GDNF

   Plasmid

   #119897

   Purpose

   Mouse GDNF In situ hybridization probe

   Depositor

   Wenqin Luo

   Article

   Fleming et al Elife. 2015 Apr 2;4:e06828. doi: 10.7554

   Insert

   glial cell line derived neurotrophic factor (Gdnf Mouse)
   

   Use

   In situ hybridization probe

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. 3788 pmko-neo A9-1

   Plasmid

   #8878

   Depositor

   Stanley Korsmeyer

   Article

   Wang et al EMBO J. 2005 Jan 26. 24(2):368-81.

   Insert

   Hox A9 (HOXA9 Human)
   

   Use

   RNAi and Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 6, 2006

   Availability

   Academic Institutions and Nonprofits only

9. 3788 pmko-neo A9-3

   Plasmid

   #8880

   Depositor

   Stanley Korsmeyer

   Article

   Wang et al EMBO J. 2005 Jan 26. 24(2):368-81.

   Insert

   Hox A9 (HOXA9 Human)
   

   Use

   RNAi and Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 6, 2006

   Availability

   Academic Institutions and Nonprofits only

10. pCMV 3xFLAG-LC3A G120

    Plasmid

    #123091

    Purpose

    Expresses 3xFLAG-LC3A G120 in mammalian cells.

    Depositor

    Robin Ketteler

    Article

    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

    Insert

    Microtubule-associated proteins 1A/1B light chain 3A (MAP1LC3A Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Deleted amino acid 121. Stop codon after G120.

    Promoter

    CMV

    Available since

    May 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMS4 (Empty insertion vector with SEC)

    Plasmid

    #154837

    Purpose

    Vector that can be modified to add gene(s) of interest to C. elegans ChrII:8420157 site via CRISPR with SEC selection

    Depositor

    Patrick Phillips

    Article

    Stevenson et al G3 (Bethesda). 2020 Aug 19. pii: g3.120.

    Insert

    SEC
    

    Use

    CRISPR and Cre/Lox

    Tags

    Expression

    Worm

    Mutation

    sqt-1(e1350)

    Promoter

    Available since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDONR223 LC3A G120

    Plasmid

    #123071

    Purpose

    Gateway entry clone encoding human MAP1LC3A G120

    Depositor

    Robin Ketteler

    Article

    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

    Insert

    Microtubule-associated proteins 1A/1B light chain 3A (MAP1LC3A Human)
    

    Use

    Gateway entry vector / entry clone

    Tags

    Expression

    Mutation

    Deleted amino acid 121. Stop codon after G120.

    Promoter

    Available since

    May 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMP71-tCD19-llo-mc38tmg

    Plasmid

    #174603

    Purpose

    expresses the extracellular and transmembrane domain of murine CD19 with a C terminal fusion of the LLO190 and minigenes of 3 neoantigens from the MC38 tumor in genes ADGPK, DPAGT and Reps1

    Depositor

    Stanley Riddell

    Article

    Veatch et al J Clin Invest. 2021 Aug 16;131(16):e1441

    Insert

    extracell transmem domains moCD19 wCterm fusion LLO190 and minigenes of 3neoantigens MC38tumor in genes ADGPK DPAGT Reps1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. ARB365

    Plasmid

    #124048

    Purpose

    Encodes pEF1a driving expression of TET3G P2A iRFP713, pEF1a expressing sadCas9 fused to mRuby2 P2A mRuby2, and an sgRNA targeting pUAS expressing mAzamiGreen in a PiggyBac destination vector

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    PB_pEF1a-tet3G-P2A-iRFP713_pEF1a-sadCAS9::mRuby2-P2A-mRuby2-SV40PA_mu6-sgUAS_pUAS-NLS::mAzamiGreen-rgPA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. p3xFLAG-CMV-RNF8-N-terminus(1-111)

    Plasmid

    #64674

    Purpose

    Expresses only N terminus of RNF8, amino acids 1 to 111

    Depositor

    Marianne Farnebo

    Article

    Henriksson et al Genes Dev. 2014 Dec 15;28(24):2726-38. d

    Insert

    Ring finger protein 8 (RNF8 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Only N terminus amino acids 1-111

    Promoter

    CMV

    Available since

    May 19, 2015

    Availability

    Academic Institutions and Nonprofits only

16. p28BIOHTEV-LIC: nsp16_SARS-CoV-2

    Plasmid

    #167846

    Purpose

    Bacterial expression of the SARS-CoV-2 protein nsp16

    Depositor

    Cheryl Arrowsmith

    Article

    Perveen et al SLAS Discov. 2021 Jan 10:247255522098504

    Insert

    nsp16
    

    Use

    Tags

    6xHis and AviTag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 16, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

17. pMuLE ENTR SV40-hCas9 L5-L2

    Plasmid

    #62134

    Purpose

    MuLE (Multiple Lentiviral Expression) Entry vector containing SV40 promoter and human codon optimized Cas9 module. Compatible with MultiSite Gateway cloning

    Depositor

    Ian Frew

    Article

    Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

    Insert

    human codon optimized Cas9
    

    Use

    CRISPR; Mule gateway entry vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SV40

    Available since

    Feb. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pJW1788

    Plasmid

    #154317

    Purpose

    CT Donor-cassette for SapTrap

    Depositor

    Jordan Ward

    Article

    Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

    Insert

    linker::TEV::BioTag::AID*::3xFLAG donor for CT slot in the SapTrap cloning system
    

    Use

    CRISPR

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Aug. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pJW1787

    Plasmid

    #154316

    Purpose

    NT Donor-cassette for SapTrap

    Depositor

    Jordan Ward

    Article

    Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

    Insert

    linker::TEV::BioTag::AID*::3xFLAG donor for NT slot in the SapTrap cloning system
    

    Use

    CRISPR

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Aug. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. ABE8.13-d

    Plasmid

    #136297

    Purpose

    expresses ABE8.13-d in mammalian cells

    Depositor

    Nicole Gaudelli

    Article

    Gaudelli et al Nat Biotechnol. 2020 Apr 13. pii: 10.103

    Insert

    ABE8.13-d
    

    Use

    Tags

    C-terminal BPNLS

    Expression

    Mammalian

    Mutation

    D10A in S. pyogenes Cas9, TadA mutations describe…

    Promoter

    Available since

    March 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pMuLE ENTR CMV-hCas9 R4-R3

    Plasmid

    #62132

    Purpose

    MuLE (Multiple Lentiviral Expression) Entry vector containing CMV promoter and human codon optimized Cas9 module Compatible with MultiSite Gateway cloning

    Depositor

    Ian Frew

    Article

    Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

    Insert

    human codon optimized Cas9
    

    Use

    CRISPR; Mule gateway entry vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pUDR211

    Plasmid

    #113870

    Purpose

    expression of Cas9 programming sgRNA3 and sgRNA4 targetting HXT8 and HXT1 respectively

    Depositor

    Jean-Marc Daran, Jack Pronk

    Article

    Wijsman et al FEMS Yeast Res. 2019 Jan 1;19(1). pii: 5

    Insert

    sgRNA3-HXT8 / sgRNA4-HXT14
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Sept. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pUDR418

    Plasmid

    #113875

    Purpose

    expression of double Cas9 programming sgRNA11 targetting STL1

    Depositor

    Jean-Marc Daran, Jack Pronk

    Article

    Wijsman et al FEMS Yeast Res. 2019 Jan 1;19(1). pii: 5

    Insert

    doble sgRNA11-STL1
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Sept. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. R0063 (pLuxR-pR)_AB

    Plasmid

    #66012

    Purpose

    MoClo Basic Part: Controllable promoter - pLuxR(pR) - luxR regulated (right promoter, weak constitutive down regulated by LuxR, C0062 in combination with homoserine lactone (HSL)) [A:R0063:B]

    Depositor

    Douglas Densmore

    Article

    Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.

    Insert

    Controllable promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pDONR223 LC3B G120

    Plasmid

    #123074

    Purpose

    Gateway entry clone encoding human MAP1LC3B G120

    Depositor

    Robin Ketteler

    Article

    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

    Insert

    Microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)
    

    Use

    Gateway entry vector / entry clone

    Tags

    Expression

    Mutation

    Deleted amino acids 121-125. Stop codon after G12…

    Promoter

    Available since

    May 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pRK5 HA-GST-Presc-mAkt1 (S473T)

    Plasmid

    #48806

    Purpose

    expresses mutant Akt1

    Depositor

    David Sabatini

    Article

    Kang et al Science. 2013 Jul 26;341(6144):1236566.

    Insert

    Akt1 (Akt1 Mouse)
    

    Use

    Tags

    HA-GST-Presc

    Expression

    Mammalian

    Mutation

    S473T

    Promoter

    CMV

    Available since

    May 2, 2014

    Availability

    Academic Institutions and Nonprofits only

27. myc-Med4 pBacPAK8

    Plasmid

    #15364

    Depositor

    Ronald Conaway, Joan Conaway

    Article

    Tomomori-Sato et al J Biol Chem. 2004 Feb 13. 279(7):5846-51

    Insert

    Med4 (MED4 N/A, Human)
    

    Use

    Baculovirus construction

    Tags

    FLAG

    Expression

    Mutation

    Promoter

    Available since

    Aug. 15, 2007

    Availability

    Academic Institutions and Nonprofits only

28. pRK5 Flag RagC(GD)/RagB(CTD) chimera

    Plasmid

    #112774

    Purpose

    express Flag-RagC (G domain) fused with with RagB (C terminal domain)

    Depositor

    Roberto Zoncu

    Article

    Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

    Insert

    Flag-RagC (G domain) fused with RagB (C terminal domain) (RRAGB Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pRK5 Flag RagB(GD)/RagC(CTD) chimera

    Plasmid

    #112773

    Purpose

    express Flag-RagB (Gdomain) fused with RagC (C terminal domain)

    Depositor

    Roberto Zoncu

    Article

    Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

    Insert

    Flag-RagB (G domain) fused with RagC (C terminal domain) (RRAGC Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pX61

    Plasmid

    #46852

    Purpose

    TOP Hupki-puro2A-Hupki-wt plasmid

    Depositor

    Monica Hollstein

    Article

    Wei et al Cell Cycle. 2011 Apr 15;10(8):1261-70. E

    Insert

    attB-Puro-2A and Hupki p53 wild type (TP53 Mouse, Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 21, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge