We narrowed to 29,109 results for: PLE;

1. pspCas9-3'UTR-Tetra-com-vector

   Plasmid

   #132553

   Purpose

   Expresses spCas9 and sgRNA scaffold with com aptamer replacing the Tetraloop

   Depositor

   Baisong Lu

   Article

   Lu et al CRISPR J. 2021 Mar 16. doi: 10.1089/cris

   Inserts

   Cas9
   sgRNA scaffold with com replacing the Tetraloop
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. HA-MBP-TEVpCS-ADGRE3 CTFdeltaTA-FLAG

   Plasmid

   #217724

   Purpose

   Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

   Depositor

   Stephen Blacklow

   Article

   Dates et al bioRxiv 2022.07.14.500097

   Insert

   ADGRE3 (ADGRE3 Human)
   

   Use

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   Deletion of residues 1-344

   Promoter

   Available since

   Aug. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. HA-MBP-TEVpCS-ADGRE3 CTF-FLAG

   Plasmid

   #217691

   Purpose

   Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

   Depositor

   Stephen Blacklow

   Article

   Dates et al bioRxiv 2022.07.14.500097

   Insert

   ADGRE3 (ADGRE3 Human)
   

   Use

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   Deletion of residues 1-338

   Promoter

   Available since

   April 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. HA-MBP-TEVpCS-ADGRE2 CTF-FLAG

   Plasmid

   #217690

   Purpose

   Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

   Depositor

   Stephen Blacklow

   Article

   Dates et al bioRxiv 2022.07.14.500097

   Insert

   ADGRE2 (ADGRE2 Human)
   

   Use

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   Deletion of residues 1-517

   Promoter

   Available since

   April 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. HA-MBP-TEVpCS-ADGRE2 CTFdeltaTA-FLAG

   Plasmid

   #217723

   Purpose

   Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

   Depositor

   Stephen Blacklow

   Article

   Dates et al bioRxiv 2022.07.14.500097

   Insert

   ADGRE2 (ADGRE2 Human)
   

   Use

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   Deletion of residues 1-523

   Promoter

   Available since

   April 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCas1_Cas2/3

   Plasmid

   #89240

   Purpose

   Plasmid contains the genes Cas1 and Cas2/3, cloned from Pseudomonas aeruginosa as a polycistronic operon. Cas1 includes an N-terminal Strep II tag and TEV protease cleavage site on Cas1

   Depositor

   Blake Wiedenheft

   Article

   Rollins et al Proc Natl Acad Sci U S A. 2017 Apr 24. p

   Insert

   Cas1 and Cas2/3
   

   Use

   Tags

   Strep II tag on Cas1

   Expression

   Mutation

   Promoter

   Available since

   May 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. px330 VRER

   Plasmid

   #101729

   Purpose

   Expresses a sgRNA and a Cas9 VRER variant that recognizes "NGCG" PAM motifs

   Depositor

   Andrew Holland

   Article

   Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

   Insert

   SpCas9 VRER
   

   Use

   Tags

   3xFLAG-NLS and NLS

   Expression

   Mammalian

   Mutation

   VRER (D1135V, G1218R, R1335E, and T1337R mutation…

   Promoter

   Available since

   Oct. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. Dapp1-PH pET11a

   Plasmid

   #71376

   Purpose

   Phosphoinositide Binding

   Depositor

   Mark Lemmon

   Article

   Ferguson et al Mol Cell. 2000 Aug;6(2):373-84.

   Insert

   Dapp1-PH (DAPP1 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Pleckstrin homology (PH) domain; aa 148-273

   Promoter

   T7

   Available since

   Feb. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

9. pRG710_lexO-HO_LexA-TF_HIS3MX

   Plasmid

   #154819

   Purpose

   Inducible expression of HO endonuclease in S. cerevisiae

   Depositor

   Lorraine Symington

   Article

   Gnugge et al Nucleic Acids Res. 2020 Oct 14. pii: 592

   Inserts

   HO endonuclease (HO Budding Yeast)
   LexA-ER-B112
   

   Use

   Tags

   FLAG

   Expression

   Yeast

   Mutation

   Promoter

   ACT1 and lexO_4-CYC1_core

   Available since

   Oct. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSLQ8611 PB-Tre3g-Fluc-DR-crIFNg-DR-crIFNg-DR-SV40PolyA/ R(EF1a- Zeo–t2A-rtTA-WPRE)

    Plasmid

    #140226

    Purpose

    Dox inducible expression of luciferase + crIFNg. Constitutive EF1a driven rtTA and zeocin resistance

    Depositor

    Stanley Qi

    Article

    Kempton et al Mol Cell. 2020 Jan 30. pii: S1097-2765(2

    Insert

    Luciferase + crIFNg
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3G

    Available since

    April 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. p458 VRER

    Plasmid

    #101728

    Purpose

    Expresses a sgRNA and a Cas9 VRER variant that recognizes "NGCG" PAM motifs

    Depositor

    Andrew Holland

    Article

    Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

    Insert

    SpCas9 VRER
    

    Use

    Tags

    3xFLAG-NLS and NLS

    Expression

    Mammalian

    Mutation

    VRER (D1135V, G1218R, R1335E, and T1337R mutation…

    Promoter

    Available since

    Oct. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. p330 VQR

    Plasmid

    #101730

    Purpose

    Expresses a sgRNA and a Cas9 VQR variant that recognizes "NGA" PAM motifs

    Depositor

    Andrew Holland

    Article

    Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

    Insert

    SpCas9 VQR
    

    Use

    Tags

    3xFLAG-NLS and NLS

    Expression

    Mammalian

    Mutation

    VQR (D1135V, R1335Q, and T1337R mutations in Cas9)

    Promoter

    Available since

    Oct. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. Grp1-PH pET11a

    Plasmid

    #71377

    Purpose

    Phosphoinositide Binding

    Depositor

    Mark Lemmon

    Article

    Ferguson et al Mol Cell. 2000 Aug;6(2):373-84.

    Insert

    Grp1-PH (Cyth3 Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Pleckstrin homology (PH) domain; aa 264-391

    Promoter

    T7

    Available since

    Feb. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

14. pX330A_D10A-1x4

    Plasmid

    #58774

    Purpose

    Expresses Cas9 nickase and gRNA

    Depositor

    Takashi Yamamoto

    Article

    Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

    Insert

    humanized S. pyogenes Cas9 (D10A) nickase
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    D10A nickase-converting mutation

    Promoter

    CBh

    Available since

    Nov. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. HA-MBP-TEVpCS-ADGRE1 CTF-FLAG

    Plasmid

    #217689

    Purpose

    Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

    Depositor

    Stephen Blacklow

    Article

    Dates et al bioRxiv 2022.07.14.500097

    Insert

    ADGRE1 (EMR1 Human)
    

    Use

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Deletion of residues 1-584

    Promoter

    Available since

    April 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pX330A_D10A-1x6

    Plasmid

    #58776

    Purpose

    Expresses Cas9 nickase and gRNA

    Depositor

    Takashi Yamamoto

    Article

    Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

    Insert

    humanized S. pyogenes Cas9 (D10A) nickase
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    D10A nickase-converting mutation

    Promoter

    CBh

    Available since

    Nov. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pIHEU_miniSOG2 T2A H3.3A-EGFP

    Plasmid

    #87411

    Purpose

    UAS vector to express Histone 3.3A GFP fusion and miniSOG2 in Drosophila

    Depositor

    Xiaokun Shu

    Article

    Makhijani et al Cell Chem Biol. 2017 Jan 19;24(1):110-11

    Insert

    mini singlet oxygen generator 2
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSumo-His6- Sumo-AtTPL(1-209)

    Plasmid

    #177856

    Purpose

    Bacterial Expression of TPL

    Depositor

    Karsten Melcher

    Article

    Ke et al Sci Adv. 2015 Jul 24;1(6):e1500107. doi:

    Insert

    TPL (TPL Mustard Weed)
    

    Use

    Tags

    6xHis-SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pPpT4- TEF2prom-TIR1F74G-FLAG

    Plasmid

    #189727

    Purpose

    Enables expression of OsTIR1(F74G) from the TEF2 promoter, used to implement the AID2 system in Komagataella phaffii (original name pLB219)

    Depositor

    Anita Emmerstorfer-Augustin

    Article

    Lehmayer et al iScience. 2022 Aug 6;25(9):104888. doi:

    Insert

    OsTIR1
    

    Use

    Tags

    FLAG

    Expression

    Yeast

    Mutation

    Promoter

    TEF2

    Available since

    Sept. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pJZC595

    Plasmid

    #62322

    Purpose

    Expresses MCP-VP64 and dCas9 in Yeast cells

    Depositor

    Stanley Qi, Wendell Lim

    Article

    Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

    Inserts

    MCP-VP64
    dCas9
    

    Use

    Tags

    VP64

    Expression

    Yeast

    Mutation

    Has nuclease-inactivating mutations in the RuvC1 …

    Promoter

    Tdh3 and pAdh

    Available since

    March 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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