We narrowed to 165,343 results for: addgene

1. LentiCRISPRv2-ACTB-C1

   Plasmid

   #169796

   Purpose

   Expresses Cas9 and guide RNA for ACTB gene in human

   Depositor

   Natsuko Chiba

   Article

   Yoshino et al Sci Rep. 2019 Feb 7;9(1):1644. doi: 10.1

   Insert

   guide RNA for ACTB gene
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   June 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCAG2IG-Hmga2

   Plasmid

   #122294

   Purpose

   Overexpression for mouse Hmga2

   Depositor

   Yukiko Gotoh

   Article

   Kishi et al Nat Neurosci. 2012 Aug;15(8):1127-33. do

   Insert

   Hmga2 (Hmga2 Mouse)
   

   Expression

   Mammalian

   Available Since

   March 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. 15x-QUAS-dTomato-T2A-TRPV1

   Plasmid

   #140945

   Purpose

   QUAS reporter line that expresses cytosolic dTomato and the rat TRPV1 ion channel, separated by T2A

   Depositor

   Leslie Vosshall

   Article

   Jove et al Neuron. 2020 Dec 23;108(6):1163-1180.e12

   Insert

   15x-QUAS-dTomato-T2A-TRPV1
   

   Available Since

   Oct. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. Chx10BP-LCL-EGFP

   Plasmid

   #73992

   Purpose

   Cre-dependent reporter plasmid for bipolar cells in the retina

   Depositor

   Connie Cepko

   Article

   Wang et al Dev Cell. 2014 Sep 8;30(5):513-27. doi:

   Insert

   Chx10BP-loxP-mCherry-loxP-EGFP
   

   Use

   Cre/Lox

   Expression

   Mammalian

   Promoter

   Chx10BP (chx10 enhancer)

   Available Since

   May 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pRosa26 R1-ccdB-R2 RexNeo PI-SceI

   Plasmid

   #24418

   Depositor

   Edward Hsiao

   Article

   Hsiao et al Stem Cell Res Ther. 2011 Mar 4. 2(2):11.

   Insert

   
   

   Use

   Mouse Targeting

   Available Since

   March 16, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. TAL2451

   Plasmid

   #36840

   Depositor

   Keith Joung

   Article

   Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

   Insert

   HMGA2-TALEN_Right (Hmga2 Human)
   

   Use

   Mammalian, t7 for in vitro mrna synthesis

   Tags

   3X Flag and WT FOKI

   Promoter

   CMV

   Available Since

   June 26, 2012

   Availability

   Academic Institutions and Nonprofits only

7. MAC_N_TBA1A

   Plasmid

   #111695

   Purpose

   MAC-tagged gene expression

   Depositor

   Markku Varjosalo

   Article

   Liu et al Nat Commun. 2018 Mar 22;9(1):1188. doi:

   Insert

   TBA1A (TUBA1A Human)
   

   Expression

   Mammalian

   Available Since

   June 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. Halo-TCAB1 KI sgRNA

   Plasmid

   #207611

   Purpose

   sgRNA for HaloTag insertion into the endogenous TCAB1 locus

   Depositor

   Jens Schmidt

   Article

   Klump et al Cell Rep. 2023 Jun 1;42(6):112577. doi:

   Insert

   CCAAAGTCTTCATACTGCAG
   

   Tags

   None

   Expression

   Mammalian

   Promoter

   CMV and U6

   Available Since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. Hmga2 3'UTR wt luciferase (Luc-wt)

   Plasmid

   #14785

   Depositor

   David Bartel

   Article

   Mayr et al Science. 2007 Mar 16. 315(5818):1576-9.

   Insert

   Luc-wt (Hmga2 Mouse)
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Mammalian

   Available Since

   April 6, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAAV-LAP2-mCherry

    Plasmid

    #159524

    Purpose

    Expresses mCherry from LAP2 promoter

    Depositor

    Esteban Engel

    Article

    Maturana et al Mol Ther Methods Clin Dev. 2020 Apr 14;1

    Insert

    mCherry
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    LAP2

    Available Since

    Sept. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. Marathon-RT for Split-PE (pEH59)

    Plasmid

    #190108

    Purpose

    Expresses Marathon-RT (human codon optimization)

    Depositor

    Keith Joung

    Article

    Grunewald et al Nat Biotechnol. 2023 Mar;41(3):337-343.

    Insert

    Marathon-RT
    

    Tags

    bpNLS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pJS183

    Plasmid

    #134142

    Purpose

    Bacterial expression of GFP-Ska1 and Ska2

    Depositor

    Iain Cheeseman

    Article

    Monda et al Curr Biol. 2017 Dec 4;27(23):3666-3675.e

    Insert

    His-GFP-human Ska1; human Ska2
    

    Tags

    GFP

    Expression

    Bacterial

    Available Since

    Feb. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pDOC-K-glmS-GFPfor

    Plasmid

    #158059

    Purpose

    Donor plasmid for insertion of eGFP and the constitutive acpP promoter into the S. Typhimurium chromosome. GFP is in the forward orientation relative to the kanamycin resistance selectable marker

    Depositor

    Mark Webber

    Article

    Holden et al Microbiology (Reading). 2020 Nov 23. doi

    Insert

    Green Fluorescent Protein optimised for excitation with UV light
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    acpP

    Available Since

    Sept. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pSN838

    Plasmid

    #184826

    Purpose

    Expresses human KIF5A(∆exon27) in mammalian cells

    Depositor

    Kyoko Chiba

    Article

    Nakano et al Genes Cells. 2022 Apr 17. doi: 10.1111/g

    Insert

    KIF5A (∆exon27) (KIF5A Human)
    

    Tags

    mScarlet

    Expression

    Mammalian

    Mutation

    ∆exon27 form

    Promoter

    CMV

    Available Since

    May 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pDM026

    Plasmid

    #216808

    Purpose

    Vector for Aspergillus CRISPR-Cas9 genetic engineering with Golden Gate cloning drop-out cassette for protospacer insertion and pyrG selectable marker.

    Depositor

    Ken Sawin

    Article

    Modaffari et al Wellcome Open Res. 2024 Oct 17;9:602. do

    Type

    Empty backbone

    Use

    CRISPR; Fungal expression

    Expression

    Bacterial

    Available Since

    Sept. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. mc2 mNeon EF1a

    Plasmid

    #206224

    Purpose

    For expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).

    Depositor

    Brett Stringer, Simon Conn

    Article

    Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Mammalian

    Available Since

    Aug. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pFYF1328 EGFP Site#3

    Plasmid

    #47513

    Purpose

    human gRNA expression vector targeting EGFP

    Depositor

    Keith Joung

    Article

    Fu et al Nat Biotechnol. 2013 Jun 23. doi: 10.103

    Insert

    gRNA-EGFP site 3
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Aug. 29, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. NDUFV3_pCSdest

    Plasmid

    #53889

    Depositor

    Roger Reeves

    Article

    Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

    Insert

    NDUFV3 (NDUFV3 Human)
    

    Expression

    Mammalian

    Promoter

    SP6 and CMV

    Available Since

    Sept. 8, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

19. EGFP-Rab32b

    Plasmid

    #140885

    Purpose

    Expression of zebrafish GFP-tagged Rab32b in mammalian cells.

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    Rab32b (rab32b Zebrafish)
    

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    Feb. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pDBTrp-LexABD-CRY2FL

    Plasmid

    #78210

    Purpose

    Use with Addgene 40615 for blue light transcription control in yeast

    Depositor

    Chandra Tucker

    Article

    Pathak et al ACS Synth Biol. 2014 Nov 21;3(11):832-8.

    Insert

    LexABD-CRY2 (CRY2 Mustard Weed)
    

    Tags

    lexA binding domain

    Expression

    Yeast

    Mutation

    contains endogenous CRY2 NLS

    Promoter

    ADH1

    Available Since

    Aug. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge