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Plasmid#214382PurposedTAG-mClover for N-terminal taggingDepositorInsertBSD-P2A-dTAG-mClover
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMS18
Plasmid#215675PurposeCas9 + guide plasmid for inserting ChrI split hygromycinR landing padDepositorInserteef-1A.1p::Cas9 + U6p::GAAATCGCCGACTTGCGAGG
UseCRISPRTagsExpressionWormMutationPromoterAvailable sinceMarch 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMS62
Plasmid#215676PurposeCas9 + guide plasmid for inserting ChrIII split hygromycinR landing padDepositorInserteef-1A.1p::Cas9 + U6p::GTCGTTCTTCCGTTCTCGGG
UseCRISPRTagsExpressionWormMutationPromoterAvailable sinceMarch 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMS77
Plasmid#215681PurposeCas9 + guide plasmid targeting ChrIII split hygromycinR landing padDepositorInserteef-1A.1p::Cas9 + U6p::GTCCAGCGGCAGATCGGCGG
UseCRISPRTagsExpressionWormMutationPromoterAvailable sinceMarch 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYSD063
Plasmid#212916PurposeEncodes mRuby2 fluorescent protein as a Type 3b' part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)DepositorInsertmRuby2
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
ABE-RA4.1
Plasmid#208306PurposeExpresses ABE-RA4.1 in mammalian cellsDepositorInsertTadA-RA4.1-SpCas9 D10A
UseTagsExpressionMammalianMutationP48A, R51H, I76F, A106V, D108G, K110R, T111H, D11…PromoterAvailable sinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
ABE-RA4.2
Plasmid#208307PurposeExpresses ABE-RA4.2 in mammalian cellsDepositorInsertTadA-RA4.2-SpCas9 D10A
UseTagsExpressionMammalianMutationP48A, R51H, I76F, A106V, D108G, K110R, T111H, D11…PromoterAvailable sinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
ABE-RA4.3
Plasmid#208308PurposeExpresses ABE-RA4.3 in mammalian cellsDepositorInsertTadA-RA4.3-SpCas9 D10A
UseTagsExpressionMammalianMutationP48A, R51H, I76F, A106V, D108G, K110R, T111H, D11…PromoterAvailable sinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
ABE-RA4.4
Plasmid#208309PurposeExpresses ABE-RA4.4in mammalian cellsDepositorInsertTadA-RA4.4-SpCas9 D10A
UseTagsExpressionMammalianMutationP48A, R51H, I76F, A106V, D108G, K110R, T111H, D11…PromoterAvailable sinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
ABE-RA4.5
Plasmid#208310PurposeExpresses ABE-RA4.5 in mammalian cellsDepositorInsertTadA-RA4.5-SpCas9 D10A
UseTagsExpressionMammalianMutationP48A, R51H, I76F, A106V, D108G, K110R, T111H, D11…PromoterAvailable sinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
ABE-RA4.6
Plasmid#208311PurposeExpresses ABE-RA4.6 in mammalian cellsDepositorInsertTadA-RA4.6-SpCas9 D10A
UseTagsExpressionMammalianMutationP48A, R51H, I76F, A106V, D108G, K110R, T111H, D11…PromoterAvailable sinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
ABE-RA5.0
Plasmid#208312PurposeExpresses ABE-RA5.0 in mammalian cellsDepositorInsertTadA-RA5.0-SpCas9 D10A
UseTagsExpressionMammalianMutationW23R, H36L, R47K, P48A, R51L, I76F, V82S, A106V, …PromoterAvailable sinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
ABE-RA5.1
Plasmid#208313PurposeExpresses ABE-RA5.1 in mammalian cellsDepositorInsertTadA-RA5.1-SpCas9 D10A
UseTagsExpressionMammalianMutationW23R, H36L, R47K, P48A, R51L, I76F, V82S, A106V, …PromoterAvailable sinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
ABE-RA5.3
Plasmid#208314PurposeExpresses ABE-RA5.3 in mammalian cellsDepositorInsertTadA-RA5.3-SpCas9 D10A
UseTagsExpressionMammalianMutationW23R, H36L, R47K, P48A, R51L, I76F, V82S, A106V, …PromoterAvailable sinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
ABE-RA5.4
Plasmid#208315PurposeExpresses ABE-RA5.4 in mammalian cellsDepositorInsertTadA-RA5.4-SpCas9 D10A
UseTagsExpressionMammalianMutationW23R, R47K, P48A, R51L, I76Y, V82S, A106V, D108G,…PromoterAvailable sinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
ABE-RA5.6
Plasmid#208317PurposeExpresses ABE-RA5.6 in mammalian cellsDepositorInsertTadA-RA5.6-SpCas9 D10A
UseTagsExpressionMammalianMutationW23R, H36L, R47K, P48A, R51L, I76Y, V82S, A106V, …PromoterAvailable sinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNZ18-CAH
Plasmid#203159PurposeReplicates in E. coli, S. cerevisiae, and A. laidlawiiDepositorInsertYeast centromere, autonomously replicating sequence, histidine auxotrophic marker
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceSept. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
FB395
Plasmid#203631PurposeTU for the expression of luciferase under a synthetic promoter containing the target sequence for gRNA1 (1xLuc).DepositorInsertR1:G1aG2b.1:mPAF:Luc:TtrpC
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceSept. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
FB396
Plasmid#203632PurposeTU for the expression of luciferase under a synthetic promoter containing two copies of the target sequence for gRNA1 (2xLuc).DepositorInsertR1:G1ab.1:mPAF:Luc:TtrpC
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceSept. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
FB397
Plasmid#203633PurposeTU for the expression of luciferase under a synthetic promoter containing three copies of the target sequence for gRNA1 (3xLuc).DepositorInsertR1:G1abc.3:mPAF:Luc:TtrpC
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceSept. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
FB312
Plasmid#203569PurposeTU for the expression of Nanoluciferase (Nluc) under the PgpdA promoter.DepositorInsertPgpdA:Nluc:Ttub
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pACT2-μ4
Plasmid#198176PurposeExpression of GAL4 transcriptional activation domain (AD)-AP-4 μ4 fusion protein in yeast (yeast two-hybrid assays)DepositorInsertAP-4 μ4
UseTagsGAL4 transcriptional activation domain (AD) fragm…ExpressionYeastMutationPromoterADH1Available sinceApril 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pACT2-μ2
Plasmid#198173PurposeExpression of GAL4 transcriptional activation domain (AD)-AP-2 μ2 fusion protein in yeast (yeast two-hybrid assays)DepositorInsertAP-2 μ2
UseTagsGAL4 transcriptional activation domain (AD) fragm…ExpressionYeastMutationPromoterADH1Available sinceMarch 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTL359
Plasmid#168420PurposepGBKT7 yeast two-hybrid plasmidDepositorInsertOsaHIP41
UseTagsExpressionYeastMutationPromoterAvailable sinceNov. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCK411.RR1
Plasmid#192644PurposeExpresses sgRNA RR1 (target mRFP CDS) on ColE1-AmpRDepositorInsertBBa_J23119-RR1
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterBBa_J23119Available sinceNov. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAV103-PRNR2-mCherry-Elk1DM-1SIM
Plasmid#190252PurposeExpression mCherry-Elk1DM in yeast cellsDepositorInsertELK1DM-SIM
UseTagsmCherryExpressionYeastMutationPromoterAvailable sinceNov. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAV105-PPAB1-GFP-Erk1R84S-1SIM
Plasmid#190253PurposeExpression Erk1-GFP-1SIM in yeast cellsDepositorInsertERK1R84S-SIM
UseTagsGFPExpressionYeastMutationERK1R84SPromoterAvailable sinceNov. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pILGFP4D42
Plasmid#185840PurposeTesting the TEF1 promoter inserted with 4 Z268 elements using a green fluorescence protein as the reporterDepositorInsertPURA3>KlURA3>TAgTEF1-PTEF1+[Z268]>yEGFP>TPGK1-TURA3
UseTagsExpressionYeastMutationNonePromoterAvailable sinceSept. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAF9D94 (pAF10C6)
Plasmid#185848PurposeExpressing artificial trans-repressor in Saccharomyces cerevisiaeDepositorInsertPHAC1>TetR>CYC8>PABF1
UseTagsExpressionYeastMutationNonePromoterAvailable sinceSept. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pILGFP10CF71
Plasmid#185860PurposeTesting the SkGAL2 promoter inserted with 4 Z268 elements using a green fluorescent protein as the reporterDepositorInsertPURA3>KlURA3>TAgTEF1- PSkGAL2+[Z268]>(BamHI)yEGFP>TURA3
UseTagsExpressionYeastMutationNonePromoterAvailable sinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pILGFP10CF72 (B9)
Plasmid#185858PurposeTesting the SkGAL2 promoter inserted with 2 PZ4 element using a green fluorescent protein as the reporterDepositorInsertPURA3>KlURA3>TAgTEF1- PSkGAL2M5(5*PZ4)>(BamHI)yEGFP>TURA3
UseTagsExpressionYeastMutationNonePromoterAvailable sinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pILGFP10CF72
Plasmid#185857PurposeTesting the SkGAL2 promoter inserted with 4 PZ4 elements using a green fluorescent protein as the reporterDepositorInsertPURA3>KlURA3>TAgTEF1- PSkGAL2M4(4*PZ4)>(BamHI)yEGFP>TURA3
UseTagsExpressionYeastMutationNonePromoterAvailable sinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pILGFP10E1B
Plasmid#185856PurposeTesting the SkGAL2 promoter inserted with 3 PZ4 elements using a green fluorescent protein as the reporterDepositorInsertPURA3>KlURA3>TAgTEF1- PSkGAL2M2(3*PZ4)>(BamHI)yEGFP>TURA3
UseTagsExpressionYeastMutationNonePromoterAvailable sinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pILGFP10E1A
Plasmid#185855PurposeTesting the SkGAL2 promoter inserted with 2 PZ4 elements using a green fluorescent protein as the reporterDepositorInsertPURA3>KlURA3>TAgTEF1- PSkGAL2M1(2*PZ4)> (BamHI)yEGFP>TURA3
UseTagsExpressionYeastMutationNonePromoterAvailable sinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pILGFP3A2 (1)
Plasmid#185851PurposeTesting the TEF1 promoter appended with 2 tetracycline riboswitch using a green fluorescence protein as the reporterDepositorInsertPURA3>KlURA3>TAgTEF1-PTEF1>2*TcRb>yEGFP>TPGK1-TURA3
UseTagsExpressionYeastMutationNonePromoterAvailable sinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCfB9341 (pgRNA_IX-1_NatMX)
Plasmid#161593PurposeEasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site IX-1DepositorInsertguiding RNA
UseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceAug. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCfB9343 (pgRNA_XV-1_NatMX)
Plasmid#161595PurposeEasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site XV-1DepositorInsertguiding RNA
UseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceAug. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCfB9358(VII-1_Markerfree_BackBone)
Plasmid#161599PurposeEasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site VII-1, (Chr VII: 438509..438490)DepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceAug. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCfB9359(VIII-1_Markerfree_BackBone)
Plasmid#161600PurposeEasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site VIII-1, (Chr VIII: 293615..293634)DepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceAug. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCfB9361(XIII-1_Markerfree_BackBone)
Plasmid#161602PurposeEasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site XIII-1, (Chr XIII: 306804..306823)DepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceAug. 9, 2022AvailabilityAcademic Institutions and Nonprofits only