We narrowed to 1,774 results for: FAST
-
Plasmid#187721PurposeExpress GST-tagged NEDD4L W2-W3-W4-HECT (349) in insect cellsDepositorInsertNEDD4L residues 349-975 (NEDD4L Human)
TagsGST-HRV 3C siteExpressionInsectMutationdeletion of residues 1-348Available SinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
Golgi-FAST-IRES-iRFP670
Plasmid#216345PurposeFor testing localization of pFAST and iRFP670 proteins to Golgi complexDepositorInsertST6Gal1_pFAST-IRES-Sys1_iRFP670
Tagsmyc for FAST and iRF670 proteinsExpressionMammalianMutationn/aPromoterCMVAvailable SinceMarch 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
Peroxisomes-FAST-IRES-iRFP670
Plasmid#216347PurposeFor testing localization of pFAST and iRFP670 proteins to peroxisomesDepositorInsertpFAST_SKL-IRES-iRFP670_PXMP2
Tagsmyc for FAST protein onlyExpressionMammalianMutationn/aPromoterCMVAvailable SinceMarch 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
Mito-FAST-IRES-iRFP670
Plasmid#216346PurposeFor testing localization of pFAST and iRFP670 proteins to mitochondriaDepositorInsertCox8_pFAST-IRES-Tomm20_iRFP670
Tagsmyc for FAST and iRF670 proteinsExpressionMammalianMutationn/aPromoterCMVAvailable SinceMarch 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFastBac1-His6-E3(1)-CUbo-TwinStrep
Plasmid#212712PurposeExpression of His6-E3(1)-CUbo-TwinStrep in insect cellsDepositorInsertE3(1)
Tags6xHis and CUbo-TwinStrepExpressionBacterialAvailable SinceMarch 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
PMP34-mApple-NFAST(low)
Plasmid#214418PurposePeroxisome localization of low-affinity NFAST fused to PMP34-AppleDepositorAvailable SinceMarch 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFastBac1-Clover4-LUMABS-S1 RBD
Plasmid#183491PurposeTo express the Clover4-LUMABS-S1 RBD sensor for SARS-CoV-2 antibody dection.DepositorInsertClover4-LUMABS-S1 RBD
ExpressionInsectAvailable SinceAug. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS2393-Tier1-PhCMV-p2a_fastFT
Plasmid#169530PurposeTier-1 vector encoding PhCMV-driven delayed-maturing fluorescent protein fluorescent timer (FT) with "fast" maturation fastFT fused to a "self-cleaving" peptide p2a (PhCMV-p2a_medFT-pA).DepositorInsertPCMV-driven P2A-fused fluorescent timer, fast maturation time
ExpressionMammalianPromoterPhCMVAvailable SinceJuly 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS2385-Tier1-PhCMV-fastFT
Plasmid#169518PurposeTier-1 vector encoding PhCMV-driven delayed-maturing fluorescent protein fluorescent timer (FT) with "fast" maturation fastFT (PhCMV-fastFT-pA).DepositorInsertPCMV-driven fluorescent timer, fast maturation time
ExpressionMammalianPromoterPhCMVAvailable SinceJune 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFASTbac-HisGST-PTPIP51(36-470)
Plasmid#170531PurposeExpresses Cleavable HisGST-tagged PTPIP51 (36-470) in Sf9DepositorInsertProtein tyrosine phosphatase interacting protein 51 (RMDN3 )
TagsGST tag and His tagExpressionInsectPromoterPolyhedrinAvailable SinceAug. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFastBac-LacI-LAP-HEC1
Plasmid#114033PurposeProduction of baculovirus with LacI-LAP-HEC1DepositorAvailable SinceAug. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFastBac(His10-Lckg2a,Y394F)
Plasmid#177893PurposeBaculo expression of His10 tag fused to human LckG2ADepositorAvailabilityAcademic Institutions and Nonprofits only -
pFastBac-GST-TEV-mCherry-TBK1
Plasmid#198033PurposeUsed for the expression and purification of mCherry labelled TBK1 (SMC Internal No.: 1675)DepositorAvailable SinceApril 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
ER-mit RspA-splitFAST (lentiviral plasmid)
Plasmid#233604PurposeExpression of ER-mit RspA-splitFAST by a single lentiviral plasmid encoding the two fragmentsDepositorInsertRspA-splitFAST
UseLentiviralPromoterCMV/SV40Available SinceApril 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pFastBac-M13-6His-KIF1C-GFP
Plasmid#130975PurposeExpression of 6His-KIF1C-GFP in insect cells for protein purification.DepositorInsertKIF1C-GFP (KIF1C Human)
Tags6His and GFPExpressionInsectMutation5 silent mutations that make this construct RNAi …PromoterPolyhedrinAvailable SinceOct. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFastBac-His-MBP-SCov2-nsp12
Plasmid#154759PurposeExpression of SARS-CoV-2 nsp12DepositorInsertSARS-CoV-2 nsp12 (ORF1ab Severe acute respiratory syndrome coronavirus 2)
ExpressionInsectPromoterPolyhedronAvailable SinceAug. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFastBac Dual α1B/β2B Tubulin
Plasmid#170553PurposeEncodes codon-optimized human α1B and β2B tubulin for expression in and purification from insect cells.DepositorTagsAP linker, GS linker (GGSGG), His10 tag, L21 enha…ExpressionInsectAvailable SinceJune 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFastBac-GFP-dynein-2(D1091–Q4307)
Plasmid#64064PurposeCodon-optimized human dynein-2 motor domain (D1091–Q4307) for baculovirus expression. 6His-ZZ-GFP N-terminal tag, TEV site to cleave 6His-ZZ.DepositorInsertCytoplasmic dynein-2 (DYNC2H1 Human)
TagsGFP, His tag, and ZZ tagExpressionInsectMutationDeleted amino acids 1-1090, extra C-terminal vali…PromoterPolyhedrinAvailable SinceApril 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFastBac Dual LIC cloning vector (5A)
Plasmid#30121DepositorTypeEmpty backboneExpressionInsectAvailable SinceJune 9, 2011AvailabilityAcademic Institutions and Nonprofits only -
pET51b-His-TEV-SNAP-tag_fast
Plasmid#167271PurposeOverexpression of the fast variant (E30R) of SNAP-tag in E. coliDepositorInsertSNAPf-tag
TagsHis x10ExpressionBacterialMutationE30RPromoterT7Available SinceApril 14, 2021AvailabilityAcademic Institutions and Nonprofits only