Addgene

We narrowed to 684 results for: PRK5

Showing: 341 - 360 of 684 results

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pRK5-HA-GST-4EBP1-Low GC

Plasmid

#69018

Purpose

expresses tagged 4EBP1

Depositor

David Sabatini

Article

Grabiner et al Cancer Discov. 2014 May;4(5):554-63. doi

Insert

EIF4EBP1 (EIF4EBP1 Human)

Use

Tags

GST and HA

Expression

Mammalian

Mutation

lowGC content, wt is ~70% GC rich. Still, it code…

Promoter

Available since

Jan. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pRK5-HA GST RagC 75L

Plasmid

#19305

Depositor

David Sabatini

Article

Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

Insert

Ras-related GTP binding C (RRAGC Human)

Use

Tags

GST and HA

Expression

Mammalian

Mutation

S75L

Promoter

Available since

Sept. 15, 2008

Availability

Academic Institutions and Nonprofits only

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pRK5-mEGFP-HMGB3-MUT

Plasmid

#194565

Purpose

Mammalian Expression of mEGFP-HMGB3 Mutant (K161Ifs*55) Fusion Protein

Depositor

Denes Hnisz

Article

Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

Insert

HMGB3 (HMGB3 Human)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

K161Ifs*55

Promoter

Available since

Feb. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pRK5-EGFP-Tau AP P301L

Plasmid

#46906

Purpose

expresses EGFP tagged Tau AP P301L in mammalian cells

Depositor

Karen Ashe

Article

Hoover et al Neuron. 2010 Dec 22;68(6):1067-81. doi:

Insert

Tau AP P301L (MAPT Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

P301L AND all 14 S/P or T/P amino acid residues (…

Promoter

CMV

Available since

July 24, 2013

Availability

Academic Institutions and Nonprofits only

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pRK5-Myc-TEAD1-Y406A

Plasmid

#33047

Depositor

Kunliang Guan

Article

Li et al Genes Dev. 2010 Feb 1;24(3):235-40.

Insert

TEAD1 (TEAD1 Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

Y406A

Promoter

Available since

Feb. 10, 2012

Availability

Academic Institutions and Nonprofits only
pRK5-HA GST RagD 77L

Plasmid

#19308

Depositor

David Sabatini

Article

Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

Insert

Ras-related GTP binding D (RRAGD Human)

Use

Tags

GST and HA

Expression

Mammalian

Mutation

S77L

Promoter

Available since

Sept. 15, 2008

Availability

Academic Institutions and Nonprofits only

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pRK5-HA GST RagB 54L

Plasmid

#19302

Depositor

David Sabatini

Article

Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

Insert

Ras-related GTP binding B (RRAGB Human)

Use

Tags

GST and HA

Expression

Mammalian

Mutation

T54L

Promoter

Available since

Sept. 15, 2008

Availability

Academic Institutions and Nonprofits only
pRK5-myc-Rac1-Q61L

Plasmid

#12983

Depositor

Gary Bokoch

Insert

Rac1 constitutively active (RAC1 Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

Q61L Constitutively active

Promoter

Available since

Oct. 27, 2006

Availability

Academic Institutions and Nonprofits only

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pRK5-HA GST RagD 121L

Plasmid

#19309

Depositor

David Sabatini

Article

Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

Insert

Ras-related GTP binding D (RRAGD Human)

Use

Tags

GST and HA

Expression

Mammalian

Mutation

Q121L

Promoter

Available since

Sept. 15, 2008

Availability

Academic Institutions and Nonprofits only

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pRK5 FLAG human DEPTOR

Plasmid

#21334

Depositor

David Sabatini

Article

Peterson et al Cell. 2009 May 13. ():.

Insert

DEPTOR (DEPTOR Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

N204S and S389N mutations (numbering according to…

Promoter

Available since

June 9, 2009

Availability

Academic Institutions and Nonprofits only

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pRK5-HA GST RagC 120L

Plasmid

#19306

Depositor

David Sabatini

Article

Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

Insert

Ras-related GTP binding C (RRAGC Human)

Use

Tags

GST and HA

Expression

Mammalian

Mutation

Q120L

Promoter

Available since

Sept. 15, 2008

Availability

Academic Institutions and Nonprofits only

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pRK5-mEGFP-SQSTM1-MUT

Plasmid

#194583

Purpose

Mammalian Expression of mEGFP-SQSTM1 Mutant (V271Sfs*41) Fusion Protein

Depositor

Denes Hnisz

Article

Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

Insert

SQSTM1 (SQSTM1 Human)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

V271Sfs*41

Promoter

Available since

Feb. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pRK5-HA GST RagA 66L

Plasmid

#19300

Depositor

David Sabatini

Article

Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

Insert

Ras-related GTP binding A (RRAGA Human)

Use

Tags

GST and HA

Expression

Mammalian

Mutation

Q66L

Promoter

Available since

Sept. 15, 2008

Availability

Academic Institutions and Nonprofits only

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Flag-MIOS(1-875) in pRK5

Plasmid

#184561

Purpose

CMV-driven expression of an N-terminally Flag-tagged MIOS (core subunit of GATOR2) — native sequence for transient expression in mammalian cells.

Depositor

Kacper Rogala

Article

Valenstein et al Nature. 2022 Jul;607(7919):610-616. doi:

Insert

MIOS (MIOS Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pRK5 CASTOR1-Flag Cterm

Plasmid

#84488

Purpose

overexpression of CASTOR1 with Flag tag

Depositor

David Sabatini

Article

Chantranupong et al Cell. 2016 Mar 24;165(1):153-64. doi: 10

Insert

CASTOR1 (GATSL3 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 24, 2016

Availability

Academic Institutions and Nonprofits only
pRK5-myc-Rac1-T17N

Plasmid

#12984

Depositor

Gary Bokoch

Insert

Rac1 DN (RAC1 Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

T17N Dominant negative

Promoter

Available since

Oct. 27, 2006

Availability

Academic Institutions and Nonprofits only

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pRK5 Flag Grb10 wt long

Plasmid

#37481

Depositor

David Sabatini

Article

Hsu et al Science. 2011 Jun 10;332(6035):1317-22.

Insert

Grb10 (GRB10 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

isoform a, 594aa

Promoter

Available since

Aug. 30, 2012

Availability

Academic Institutions and Nonprofits only

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pRK5-HA GST RagA 21L

Plasmid

#19299

Depositor

David Sabatini

Article

Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

Insert

Ras-related GTP binding A (RRAGA Human)

Use

Tags

GST and HA

Expression

Mammalian

Mutation

T21L

Promoter

Available since

Sept. 15, 2008

Availability

Academic Institutions and Nonprofits only
[pSK729] pRK5 HA-FLCN(R164A)

Plasmid

#136147

Purpose

mammalian expression of FLCN

Depositor

Kuang Shen, David Sabatini

Article

Shen et al Cell. 2019 Nov 27;179(6):1319-1329.e8. d

Insert

FLCN (FLCN Human)

Use

Tags

HA

Expression

Mammalian

Mutation

R164A

Promoter

Available since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pRK5-PLA2G15-6xHis S198A

Plasmid

#213605

Purpose

Expresses PLA2G15-6xHis S198A in mammalian cells

Depositor

Monther Abu-Remaileh

Article

Nyame et al Mol Cell. 2024 Apr 4;84(7):1354-1364.e9.

Insert

Phospholipase A2 Group XV (PLA2G15 Human)

Use

Tags

6xHis

Expression

Mammalian

Mutation

Changed serine 198 to alanine

Promoter

CMV

Available since

March 6, 2024

Availability

Academic Institutions and Nonprofits only

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