Addgene

We narrowed to 3,266 results for: aaas

Showing: 341 - 360 of 3266 results

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MYMH 35

Plasmid

#138032

Purpose

Encodes Ribosome Binding Site (RBS) variant using GFP as a reporter gene

Depositor

Meng How Tan

Article

Teh et al ACS Synth Biol. 2019 Apr 19;8(4):708-723

Insert

pSEVA-331Bb-6GT-GFP

Use

Tags

Expression

Bacterial

Mutation

AAA GAT GAG AAA

Promoter

Available since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

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MYMH 33

Plasmid

#138031

Purpose

Encodes Ribosome Binding Site (RBS) variant using GFP as a reporter gene

Depositor

Meng How Tan

Article

Teh et al ACS Synth Biol. 2019 Apr 19;8(4):708-723

Insert

pSEVA-331Bb-6GC-GFP

Use

Tags

Expression

Bacterial

Mutation

AAA GAC GAG AAA

Promoter

Available since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

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MYMH 31

Plasmid

#138030

Purpose

Encodes Ribosome Binding Site (RBS) variant using GFP as a reporter gene

Depositor

Meng How Tan

Article

Teh et al ACS Synth Biol. 2019 Apr 19;8(4):708-723

Insert

pSEVA-331Bb-5AT-GFP

Use

Tags

Expression

Bacterial

Mutation

AAA GTG GAG AAA

Promoter

Available since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

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MYMH 29

Plasmid

#138029

Purpose

Encodes Ribosome Binding Site (RBS) variant using GFP as a reporter gene

Depositor

Meng How Tan

Article

Teh et al ACS Synth Biol. 2019 Apr 19;8(4):708-723

Insert

pSEVA-331Bb-5AC-GFP

Use

Tags

Expression

Bacterial

Mutation

AAA GCG GAG AAA

Promoter

Available since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

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MYMH 27

Plasmid

#138028

Purpose

Encodes Ribosome Binding Site (RBS) variant using GFP as a reporter gene

Depositor

Meng How Tan

Article

Teh et al ACS Synth Biol. 2019 Apr 19;8(4):708-723

Insert

pSEVA-331Bb-5AG-GFP

Use

Tags

Expression

Bacterial

Mutation

AAA GGG GAG AAA

Promoter

Available since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
MYMH 25

Plasmid

#138027

Purpose

Encodes Ribosome Binding Site (RBS) variant using GFP as a reporter gene

Depositor

Meng How Tan

Article

Teh et al ACS Synth Biol. 2019 Apr 19;8(4):708-723

Insert

pSEVA-331Bb-4GA-GFP

Use

Tags

Expression

Bacterial

Mutation

AAA AAG GAG AAA

Promoter

Available since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

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MYMH 23

Plasmid

#138026

Purpose

Encodes Ribosome Binding Site (RBS) variant using GFP as a reporter gene

Depositor

Meng How Tan

Article

Teh et al ACS Synth Biol. 2019 Apr 19;8(4):708-723

Insert

pSEVA-331Bb-4GT-GFP

Use

Tags

Expression

Bacterial

Mutation

AAA TAG GAG AAA

Promoter

Available since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

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MYMH 21

Plasmid

#138025

Purpose

Encodes Ribosome Binding Site (RBS) variant using GFP as a reporter gene

Depositor

Meng How Tan

Article

Teh et al ACS Synth Biol. 2019 Apr 19;8(4):708-723

Insert

pSEVA-331Bb-4GC-GFP

Use

Tags

Expression

Bacterial

Mutation

AAA CAG GAG AAA

Promoter

Available since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

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pJC45BS104 Y257W

Plasmid

#1303

Depositor

Susan Lindquist

Article

Cashikar et al Mol Cell 2002 Apr;9(4):751-60.

Insert

Hsp104 Y257W (HSP104 Budding Yeast)

Use

Tags

10X His tag

Expression

Bacterial

Mutation

Changed Tyr 257 to Trp

Promoter

Available since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only

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pJC45BS104 Y507W

Plasmid

#1305

Depositor

Susan Lindquist

Article

Cashikar et al Mol Cell 2002 Apr;9(4):751-60.

Insert

Hsp104 Y507W (HSP104 Budding Yeast)

Use

Tags

10X His tag

Expression

Bacterial

Mutation

Changed Tyr 507 to Trp

Promoter

Available since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only

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HA, EGFP Spastin M87 with exon 4

Plasmid

#167542

Purpose

For doxycycline inducible mammalian expression of EGFP tagged Spastin M87 with exon 4.

Depositor

Tarun Kapoor

Article

Cupido et al Nat Chem Biol. 2019 May;15(5):444-452. d

Insert

Spastin M87 with exon 4 (SPAST Human)

Use

Tags

EGFP and HA tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 5, 2021

Availability

Academic Institutions and Nonprofits only

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KBB394

Plasmid

#185086

Purpose

NMA111 with both nuclear localization signals mutated to alanines fused to GFP (mutagenesis of KBB280)

Depositor

Ken Belanger

Article

Belanger et al J Cell Sci. 2009 Nov 1;122(Pt 21):3931-4

Insert

NMA111

Use

Tags

GFP

Expression

Yeast

Mutation

Nma111 KKR 9-11 AAA; KRK 28-30 AAA

Promoter

Available since

July 21, 2022

Availability

Academic Institutions and Nonprofits only

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Mdn1-FL

Plasmid

#171602

Purpose

Expresses full length S. pombe Mdn1 in insect cells.

Depositor

Tarun Kapoor

Article

Mickolajczyk et al Proc Natl Acad Sci U S A. 2020 Aug 4;117

Insert

Mdn1

Use

Tags

His6 tag

Expression

Insect

Mutation

Promoter

Available since

July 19, 2021

Availability

Academic Institutions and Nonprofits only

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pCDF-1b-RFC140ΔN555

Plasmid

#175043

Purpose

Overexpression of human (Rfc1) p140ΔN555 in E.coli

Depositor

Brian Kelch

Article

Gaubitz et al Proc Natl Acad Sci U S A. 2020 Sep 22;11

Insert

Replication factor C subunit 1 (RFC1 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

Jan. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pET15b His-p97

Plasmid

#169020

Purpose

Expresses His-p97 in bacteria

Depositor

Hemmo Meyer

Article

Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

Insert

p97

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 7, 2021

Availability

Academic Institutions and Nonprofits only

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HA, EGFP Spastin M87 with exon 4, N386C

Plasmid

#167543

Purpose

For doxycycline inducible mammalian expression of EGFP tagged Spastin M87 with exon 4, with N386C mutation.

Depositor

Tarun Kapoor

Article

Cupido et al Nat Chem Biol. 2019 May;15(5):444-452. d

Insert

Spastin M87 with exon 4 (SPAST Human)

Use

Tags

EGFP and HA tag

Expression

Mammalian

Mutation

N386C

Promoter

CMV

Available since

May 5, 2021

Availability

Academic Institutions and Nonprofits only

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SNAP-MIDAS

Plasmid

#171604

Purpose

Expresses the SNAP-tagged MIDAS domain of S. pombe Mdn1 (a.a. 4381-4717) in bacteria.

Depositor

Tarun Kapoor

Article

Mickolajczyk et al Proc Natl Acad Sci U S A. 2020 Aug 4;117

Insert

MIDAS domain of S. pombe Mdn1 (a.a. 4381-4717)

Use

Tags

His6 tag and SNAP tag

Expression

Bacterial

Mutation

Promoter

Available since

July 19, 2021

Availability

Academic Institutions and Nonprofits only

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KBB430

Plasmid

#185091

Purpose

NMA111-GFP with first nuclear localization signal mutated (amino acids 28 - 30, KRK -> AAA)

Depositor

Ken Belanger

Article

Belanger et al J Cell Sci. 2009 Nov 1;122(Pt 21):3931-4

Insert

NMA111

Use

Tags

GFP

Expression

Yeast

Mutation

First nuclear localization signal mutated (amino …

Promoter

Available since

July 29, 2022

Availability

Academic Institutions and Nonprofits only

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KBB428

Plasmid

#185090

Purpose

NMA111-GFP with first nuclear localization signal mutated (amino acids 9 - 11, KKR -> AAA)

Depositor

Ken Belanger

Article

Belanger et al J Cell Sci. 2009 Nov 1;122(Pt 21):3931-4

Insert

NMA111

Use

Tags

GFP

Expression

Yeast

Mutation

First nuclear localization signal mutated (amino …

Promoter

Available since

July 27, 2022

Availability

Academic Institutions and Nonprofits only

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Mdn1-deltaC

Plasmid

#171603

Purpose

Expresses S. pombe Mdn1 a.a. 1-3911 in insect cells.

Depositor

Tarun Kapoor

Article

Mickolajczyk et al Proc Natl Acad Sci U S A. 2020 Aug 4;117

Insert

Mdn1

Use

Tags

His6 tag

Expression

Insect

Mutation

C-terminal deletion; a.a. 1-3911 only

Promoter

Available since

July 19, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge