We narrowed to 3,917 results for: LYC

1. pLenti-PGK-flag-RAP1B-V12-puro

   Plasmid

   #156168

   Purpose

   Lentiviral vector for expression of flag-RAP1B-V12, with puromycin selection

   Depositor

   Roland Friedel

   Article

   Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

   Insert

   RAP1B (RAP1B Bovine)
   

   Use

   Lentiviral

   Tags

   2xFLAG

   Expression

   Mammalian

   Mutation

   changed Glycine 12 to Valine

   Promoter

   human PGK

   Available since

   July 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3-Flag::UbvG08 P69L, L70V, I44A, deltaGG

   Plasmid

   #74940

   Purpose

   Ubiquitin variant with mutations L69P and V70L reverted to wild type (i53-DM mutant)

   Depositor

   Daniel Durocher

   Article

   Canny et al Nat Biotechnol. 2018 Jan;36(1):95-102. d

   Insert

   Ubiquitin
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   UbvG08 with I44A, P69L, and L70V mutations and no…

   Promoter

   CMV

   Available since

   May 13, 2016

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. R620-M14-303: CMV51p> SARS-CoV-2 S(1-1213)-2P-T4f-His6 B.1.351

   Plasmid

   #170198

   Purpose

   Mammalian expression of SARS-CoV-2 soluble spike trimer protein (B.1.351 variant)

   Depositor

   Dominic Esposito

   Article

   Esposito et al Protein Expr Purif. 2020 Jun 4;174:10568

   Insert

   SARS-CoV-2 S (spike) B.1.351 (S SARS-CoV-2)
   

   Use

   Tags

   His6

   Expression

   Mammalian

   Mutation

   L18F, D80A, D215G, Δ(242-244), R246I, K417N, E484…

   Promoter

   CMV51

   Available since

   May 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDONR223-PGK2

   Plasmid

   #23625

   Depositor

   David Root, William Hahn

   Article

   Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

   Insert

   PGK2 (PGK2 Human)
   

   Use

   Gateway donor vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 30, 2010

   Availability

   Academic Institutions and Nonprofits only

5. Spike Display_Part 2 Spacer

   Plasmid

   #172730

   Purpose

   Encodes Part 2 of HexaPro-D614G spike to be used in the Spike Display system

   Depositor

   IIya Finkelstein

   Article

   Javanmardi et al Mol Cell. 2021 Dec 16;81(24):5099-5111.e

   Insert

   SARS-CoV-2 S HexaPro-D614G (S Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Ectodomain only (AAs 1-1208); 682-685 (furin site…

   Promoter

   Available since

   Aug. 17, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pCRISPaint-mScarlet-PuroR [M1G]

   Plasmid

   #171809

   Purpose

   Universal donor plasmid for CRISPitope method encoding mScarlet fluorescent protein and puromycin resistance cassette [p.M1G]

   Depositor

   Michael Hoelzel

   Article

   Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.

   Insert

   mScarlet-T2A-PuroR
   

   Use

   Gene tagging

   Tags

   Expression

   Mutation

   PuroR: Changed Methionin 1 to Glycine.

   Promoter

   Available since

   July 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSBbi-moxGFP-P2A-T2A-cMyc-Puro

   Plasmid

   #176892

   Purpose

   Sleeping Beauty transposon-based vector for mammalian polycistronic expression of moxGFP, c-Myc and an additional gene of interest

   Depositor

   Jorge Luis Arias-Arias

   Article

   Acon et al iScience. 2021 Nov 8;24(12):103407. doi:

   Insert

   moxGFP-P2A-T2A-c-Myc
   

   Use

   Transposon

   Tags

   Myc and moxGFP

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a/RPBSA

   Available since

   Nov. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pVRC8400-SARS-CoV-2-S2P-B.1.617.2-AVI

   Plasmid

   #176334

   Purpose

   Expresses SARS-CoV-2 S2P protein from the B.1.617.2 variant with single chain Fc, HRV3C protease cleavage site, and AVI tag

   Depositor

   Peter Kwong

   Article

   Teng et al PLoS One. 2022 May 24;17(5):e0268767. do

   Insert

   SARS-CoV-2-S2P-B.1.617.2 (S )
   

   Use

   Tags

   AVI, HRV3C, and scFc

   Expression

   Mammalian

   Mutation

   T19R, G142D, del156-157, R158G, L452R, T478K, D61…

   Promoter

   Available since

   March 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. A3Ai E72A-Cas9n-UGI-NLS

   Plasmid

   #109430

   Purpose

   Expresses catalytically inactive human APOBEC3A with an L1 intron fused to Cas9n, Uracil DNA Glycosylase Inhibitor, with a nuclear localization signal.

   Depositor

   Reuben Harris

   Article

   St Martin et al Nucleic Acids Res. 2018 May 9. pii: 4994

   Insert

   Apolipoprotein B mRNA Editing Enzyme, Catalytic Polypeptide-Like 3A E72A Catalytic Mutant (APOBEC3A Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Insertion of an L1 intron into the coding sequenc…

   Promoter

   CMV

   Available since

   July 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAGGS-Omicron.BA.5-RBD

    Plasmid

    #213478

    Purpose

    Mammalian cell expression of SARS-CoV-2 Spike RBD protein of OMICRON.BA.5 variant

    Depositor

    Priyamvada Acharya

    Article

    Zhang et al bioRxiv [Preprint]. 2024 Mar 12:2024.02.

    Insert

    pCAGGS/Omicron.BA.5 RBD (S )
    

    Use

    Tags

    6 His Tag

    Expression

    Mammalian

    Mutation

    G339D,R346T,S371F,S373P,S375F,T376A,D405N,R408S,K…

    Promoter

    Available since

    March 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAGGS/EG5-RBD

    Plasmid

    #213410

    Purpose

    Mammalian cell expression of SARS-CoV-2 Spike RBD protein of OMICRON.EG5 variant

    Depositor

    Priyamvada Acharya

    Article

    Zhang et al bioRxiv [Preprint]. 2024 Mar 12:2024.02.

    Insert

    pCAGGS/EG5-RBD (S )
    

    Use

    Tags

    6 His Tag

    Expression

    Mammalian

    Mutation

    G339H,R346T,L368I,S371F,S373P,S375F,T376A,D405N,R…

    Promoter

    Available since

    March 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. CD59-CD4d3+4-bio

    Plasmid

    #73117

    Purpose

    EXPRESs plasmid for human erythrocyte surface proteins encoding CD59 with rat CD4d3+4-bio

    Depositor

    Gavin Wright

    Article

    Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

    Insert

    CD59 glycoprotein (CD59, MIC11 Human)
    

    Use

    Tags

    biotinylation peptide and ratCD4d3+4

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. 6xHis-TEV-mCherry-LC3B-Gly (Microtubule-associated proteins 1A/1B light chain 3B)

    Plasmid

    #169168

    Purpose

    Conjugatable form of LC3B lacking 5 C-terminal amino acids (MKLSV), with C-terminal Glycine120 exposed for lipidation reaction.

    Depositor

    Sascha Martens

    Article

    Zaffagnini et al EMBO J. 2018 Mar 1;37(5). pii: embj.2017

    Insert

    MAP1LC3B (MAP1LC3B Human)
    

    Use

    Tags

    6X Histidine Tag, TEV cleavage site, mCherry

    Expression

    Bacterial

    Mutation

    Promoter

    T7 lac promoter

    Available since

    May 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pαH-S-GSAS-P1-like

    Plasmid

    #173789

    Purpose

    Mammalian expression of SARS-CoV-2 Spike protein P-1-like variant (K417N)

    Depositor

    Priyamvada Acharya

    Article

    Gobeil et al Science. 2021 Jun 24. pii: science.abi62

    Insert

    Spike S-GSAS- P-1-like variant (K417N) (S Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    HRV3C cleavage site, 8X His tag, 2X Strep-Tag II

    Expression

    Mammalian

    Mutation

    Ectodomain (AAs 1-1208); 682-685 (furin site = RR…

    Promoter

    Available since

    Aug. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pVRC8400-SARS-CoV-2-RBD-BA.2-AVI

    Plasmid

    #184533

    Purpose

    Expresses SARS-CoV-2 RBD domain from the BA.2 variant with single chain Fc, HRV3C protease cleavage site, and AVI tag

    Depositor

    Peter Kwong

    Article

    Teng et al PLoS One. 2022 May 24;17(5):e0268767. do

    Insert

    SARS-CoV-2-RBD-BA.2 (S )
    

    Use

    Tags

    AVI, HRV3C, and scFc

    Expression

    Mammalian

    Mutation

    G339D, S371F, S373P, S375F, T376A, D405N, R408S, …

    Promoter

    Available since

    May 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pVRC8400-SARS-CoV-2-RBD-E484K-AVI

    Plasmid

    #176444

    Purpose

    Expresses SARS-CoV-2 RBD domain containing E484K mutation with single chain Fc, HRV3C protease cleavage site, and AVI tag

    Depositor

    Peter Kwong

    Article

    Teng et al PLoS One. 2022 May 24;17(5):e0268767. do

    Insert

    SARS-CoV-2-RBD-E484K (S )
    

    Use

    Tags

    AVI, HRV3C, and scFc

    Expression

    Mammalian

    Mutation

    E484K

    Promoter

    Available since

    March 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET30-2-GPD1L

    Plasmid

    #83912

    Purpose

    stable overexpression

    Depositor

    David Sabatini

    Article

    Pacold et al Nat Chem Biol. 2016 Jun;12(6):452-8. doi

    Insert

    glycerol-3-phosphate dehydrogenase 1-like (GPD1L Human)
    

    Use

    Tags

    6x His tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLenti-PGK-RAP2A-V12-neo

    Plasmid

    #156178

    Purpose

    Lentiviral vector for expression of RAP2A-V12, with neomycin selection

    Depositor

    Roland Friedel

    Article

    Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

    Insert

    RAP2A (RAP2A Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    changed Glycine 12 to Valine

    Promoter

    human PGK

    Available since

    July 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLenti-PGK-flag-RAP1B-V12-neo

    Plasmid

    #156175

    Purpose

    Lentiviral vector for expression of flag-RAP1B-V12, with neomycin selection

    Depositor

    Roland Friedel

    Article

    Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

    Insert

    RAP1B (RAP1B Bovine)
    

    Use

    Lentiviral

    Tags

    2xFLAG

    Expression

    Mammalian

    Mutation

    changed Glycine 12 to Valine

    Promoter

    human PGK

    Available since

    July 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. R620-M12-303: CMV51p> SARS-CoV-2 S(1-1208)-2P-T4f-3C-His8-Strep2x2 B.1.351

    Plasmid

    #170196

    Purpose

    Mammalian expression of SARS-CoV-2 soluble spike trimer protein (B.1.351 variant)

    Depositor

    Dominic Esposito

    Article

    Esposito et al Protein Expr Purif. 2020 Jun 4;174:10568

    Insert

    SARS-CoV-2 S (spike) B.1.351 (S SARS-CoV-2)
    

    Use

    Tags

    3C-His8-Strep2x2

    Expression

    Mammalian

    Mutation

    L18F, D80A, D215G, Δ(242-244), R246I, K417N, E484…

    Promoter

    CMV51

    Available since

    Nov. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge