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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 8,371 results for: BLI;

Showing: 7161 - 7200 of 8371 results

  1. GRLF1

    Plasmid
    #36195
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    GRLF1 (Codon Devices Synthesized) (ARHGAP35 Human)
    Use
    Tags
    His tag with TEV cleavage
    Expression
    Bacterial
    Mutation
    Contains the Ras homolog domain only (amino acid …
    Promoter
    Available since
    June 19, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits

  2. pcDNA3_HLA-cmyc-GRM3 C127A

    Plasmid
    #140451
    Purpose
    Mammalian expression plasmid for c-myc-tagged mGluR3 C127A mutant
    Depositor
    Erik Procko
    Insert
    GRM3 (GRM3 Human)
    Use
    Tags
    Signal/leader sequence from HLA class I histocomp…
    Expression
    Mammalian
    Mutation
    Cysteine 127 to alanine
    Promoter
    Available since
    July 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    140451-plasmid-map-sequence-id-291874

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  16. pGEX2TK-YAP-WW1-RRK

    Plasmid
    #104306
    Purpose
    bacterial expression of RKK mutant WW domain 1 of YAP; RRK mutant binds to PPxY where Y is phosphorylated
    Depositor
    Marius Sudol
    Insert
    YAP WW1 domain (YAP1 Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    WW domain 1 RRK mutant (TTAAATCACATCGATCAG to AGA…
    Promoter
    tac
    Available since
    Jan. 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    104306-plasmid-map-sequence-id-201230

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  18. pLSU4mg10

    Plasmid
    #126079
    Purpose
    Plant transformation vector for stable expression of mitochondria targeted GFP1-10 in plant cells
    Depositor
    Ralf-Bernd Klösgen
    Article
    Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs
    Insert
    mitochondria Rieske iron sulphur protein (1-100 aa)
    Use
    Binary
    Tags
    GFP1-10
    Expression
    Plant
    Mutation
    Promoter
    'long' CaMV35S
    Available since
    Aug. 26, 2019
    Availability
    Academic Institutions and Nonprofits only
    126079-plasmid-map-sequence-id-254915

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  19. TET-pLKO-neo CXCR4-sh3

    Plasmid
    #163743
    Purpose
    Lentivirus for inducible knockdown of CXCR4 (human)
    Depositor
    Roland Friedel
    Insert
    CXCR4 (CXCR4 Human)
    Use
    Lentiviral and RNAi
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    TRE
    Available since
    Jan. 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    163743-plasmid-map-sequence-id-322871

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  20. TET-pLKO-neo CXCR4-sh1

    Plasmid
    #163741
    Purpose
    Lentivirus for inducible knockdown of CXCR4 (human)
    Depositor
    Roland Friedel
    Insert
    CXCR4 (CXCR4 Human)
    Use
    Lentiviral and RNAi
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    TRE
    Available since
    April 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    163741-plasmid-map-sequence-id-330897

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  21. TET-pLKO-neo CXCR4-sh2

    Plasmid
    #163742
    Purpose
    Lentivirus for inducible knockdown of CXCR4 (human)
    Depositor
    Roland Friedel
    Insert
    CXCR4 (CXCR4 Human)
    Use
    Lentiviral and RNAi
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    TRE
    Available since
    Jan. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    163742-plasmid-map-sequence-id-322780

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  24. pB6-Csrp2-T2A-sfGFP-iCre-ERT2-FNF-DTA

    Plasmid
    #140270
    Purpose
    B6J Csrp2-T2A-sfGFP-iCre-ERT2 allele targeting vector, low copy number
    Depositor
    Mario Capecchi
    Insert
    Csrp2 (Csrp2 Mouse)
    Use
    Cre/Lox and Mouse Targeting
    Tags
    C-terminal fusion of T2A-sfGFP-iCre-ERT2
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    140270-plasmid-map-sequence-id-317909

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  27. L3MBTL3 (3UT1)

    Plasmid
    #36901
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    L3MBTL3 (L3MBTL3 Human)
    Use
    Tags
    His tag and Streptavidin-Binding Peptide (SBP)-Ta…
    Expression
    Bacterial
    Mutation
    contains amino acid residues 228-551 only
    Promoter
    Available since
    June 21, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits

  28. pGS1011

    Plasmid
    #63783
    Purpose
    Vector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal Strep tag II followed by TRP1 selection marker
    Depositor
    Monika Golas, Bjoern Sander
    Article
    Rai et al Mol Biotechnol. 2014 Jun 27.
    Insert
    Strep-tag II followed by TRP1 selection marker
    Use
    E. coli cloning vector
    Tags
    Strep-tag II
    Expression
    Mutation
    Promoter
    none
    Available since
    April 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    63783-plasmid-map-sequence-id-106469

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  30. pLD-puro-Cc-IFIT5_K206R-VA

    Plasmid
    #191224
    Purpose
    Lentiviral expression of human IFIT5_K206R in mammalian cells
    Depositor
    Mohan Babu
    Insert
    interferon-induced protein with tetratricopeptide repeats 5 (IFIT5 Human)
    Use
    Lentiviral
    Tags
    3xFlag-TEVx2-6xHis-Strep x2 tag and VA tag
    Expression
    Mammalian
    Mutation
    changed Lysine 206 to Arginine (K206R)
    Promoter
    CMV
    Available since
    April 30, 2025
    Availability
    Academic Institutions and Nonprofits only
    191224-plasmid-map-sequence-id-438791

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  31. rTetR-GFP-p65

    Plasmid
    #232633
    Purpose
    p65 activation domain coupled to reverse Tet repressor; labeled with GFP; binds tetO sites upon doxycycline addition
    Depositor
    Karsten Rippe
    Insert
    rTetR-GFP-p65 (RELA Synthetic, Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    none
    Promoter
    CMV
    Available since
    March 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    232633-plasmid-map-sequence-id-463626

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  32. pdCas9-GFP-p65

    Plasmid
    #232630
    Purpose
    p65 activation domain coupled to catalytically inactive dCas9 for localization; contains GFP for labeling and as a spacer
    Depositor
    Karsten Rippe
    Insert
    dCas9-GFP-p65 (RELA Synthetic, Human)
    Use
    CRISPR
    Tags
    NLS
    Expression
    Mammalian
    Mutation
    none
    Promoter
    CMV
    Available since
    March 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    232630-plasmid-map-sequence-id-463624

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  33. Kif3A-EGFP-SNAP

    Plasmid
    #229852
    Purpose
    Kif3A (aa1-342)-Kif1A neck linker(17aa) fused to Kin1 coiled coil (aa345-406)-EGFP-SNAP-his. This Kif3A was used to link an oligo to the motor using the SNAP tag for single molecule experiments
    Depositor
    William Hancock
    Insert
    Kif3A (Kif3a Mouse)
    Use
    Tags
    6xHis, EGFP, and SNAP-tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7 promoter
    Available since
    Feb. 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    229852-plasmid-map-sequence-id-460709

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  34. pLANT-2b

    Plasmid
    #231487
    Purpose
    Expresses His6-SUMO^NIT-FraB where "NIT" stands for "no internal translation", reflecting the mutation of the two Shine-Dalgarno-like sequences within the SUMO tag.
    Depositor
    Venkat Gopalan
    Inserts
    SUMO^NIT (SMT3 Budding Yeast)
    FraB
    Use
    Tags
    His6 tag and His6-SUMO^NIT
    Expression
    Bacterial
    Mutation
    Deleted amino acids 1-6 (MEPEES). and M83L, E84Q,…
    Promoter
    T7 promoter
    Available since
    Feb. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    231487-plasmid-map-sequence-id-460673

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  35. E547* FL USP7

    Plasmid
    #229001
    Purpose
    E547-mutant of human USP7
    Depositor
    Irina Bezsonova
    Insert
    USP7 1-546 (USP7 Human)
    Use
    Tags
    6xHis cleavable with thrombin
    Expression
    Bacterial
    Mutation
    E547 mutated to stop codon
    Promoter
    T7
    Available since
    Jan. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    229001-plasmid-map-sequence-id-458328

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  36. pCAGGS-Twin-strep-TEV-DGKδ2

    Plasmid
    #223720
    Purpose
    Express human diacylglycerol kinase delta 2 protein (N-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-fusion protein (ENLYFQGS-WSHPQFEK-(GGGS)2-GGSA-WSHPQFEK)) in mammalian cell
    Depositor
    Chiaki Murakami
    Insert
    DGKD (DGKD Human)
    Use
    Tags
    TEV cleavable site and Twin-Strep-tag
    Expression
    Mammalian
    Mutation
    deleted start codon
    Promoter
    CAG and chicken β-actin promoter
    Available since
    Jan. 8, 2025
    Availability
    Academic Institutions and Nonprofits only
    223720-plasmid-map-sequence-id-457431

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  37. pCAGGS-PHOSPHO1-TEV-Twin-Strep

    Plasmid
    #222015
    Purpose
    Express human PHOSPHO1 protein (C-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-fusion protein (ENLYFQGS-WSHPQFEK-(GGGS)2-GGSA-WSHPQFEK)) in mammalian cell
    Depositor
    Chiaki Murakami
    Insert
    PHOSPHO1 (PHOSPHO1, PHOSPHO1 Human)
    Use
    Tags
    TEV cleavable site and Twin-Strep-tag
    Expression
    Mammalian
    Mutation
    stop codon (TGA) is deleted
    Promoter
    chicken β-actin promoter
    Available since
    Jan. 8, 2025
    Availability
    Academic Institutions and Nonprofits only
    222015-plasmid-map-sequence-id-457427

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  38. pWR58

    Plasmid
    #202795
    Purpose
    integrating bioluminescene reporter for Cas9 activity in S. stipitis
    Depositor
    J. Brian Robertson
    Article
    Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:
    Inserts
    P-TDH3/coCBG(first portion)-YUM1
    P-TEF1/ShBle/T-ACT1
    YUM1-coCBG(last portion)/T-ADH2
    URA3 and flanking sequence
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Dec. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    202795-plasmid-map-sequence-id-455380

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  39. pDGB3_alpha2_BeYDV geminino 2.0 (no ATG)-eGFP (GB5178)

    Plasmid
    #225443
    Purpose
    Geminino 2.0-no ATG on CDS. BeYDV LIR1 + 2nd half intron ICON MP + eGFP w/o ATG + t35S:SIR + P35s + 1st half of intron from ICON MP + BeYDV LIR1.
    Depositor
    Diego Orzaez
    Insert
    BeYDV LIR1 + 2nd half intron ICON MP + eGFP w/o ATG + t35S:SIR + P35s + 1st half of intron from ICON MP + BeYDV LIR1
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    Available since
    Oct. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    225443-plasmid-map-sequence-id-447736

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  40. pTipi2.1_Anti-GCN4 [IPI-C11L34] Mouse/Rabbit LC kappa

    Plasmid
    #221354
    Purpose
    Mammalian expression of Anti-GCN4 [IPI-C11L34] Mouse/Rabbit LC kappa; to be used with Anti-GCN4 [IPI-C11L34] heavy chain (Plasmid 221353) to make the antibody.
    Depositor
    Institute for Protein Innovation (IPI)
    Insert
    Anti-GCN4 [IPI-C11L34] Mouse/Rabbit LC kappa
    Use
    Affinity Reagent/ Antibody
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 1, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    221354-plasmid-map-sequence-id-436804

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Showing: 7161 - 7200 of 8371 results