We narrowed to 3,813 results for: biorxiv

1. pRetroQ-GTSE1-acGFP-S91, 262, 454, 724A

   Plasmid

   #224741

   Purpose

   Retroviral vector for delivery and expression of acGFP1-tagged GTSE1 protein with S91, 262, 454, 724A mutation

   Depositor

   Michele Pagano

   Article

   García-Vázquez et al bioRxiv 2024.06.26.600797

   Insert

   GTSE1 (GTSE1 Human)
   

   Use

   Retroviral

   Tags

   acGFP

   Expression

   Mutation

   Serine 91, 262, 454, 724 to Alanine

   Promoter

   CMV

   Available since

   Dec. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRetroQ-GTSE1-acGFP-S91, 262, 454, 724D

   Plasmid

   #224742

   Purpose

   Retroviral vector for delivery and expression of acGFP1-tagged GTSE1 protein with S91, 262, 454, 724D mutation

   Depositor

   Michele Pagano

   Article

   García-Vázquez et al bioRxiv 2024.06.26.600797

   Insert

   GTSE1 (GTSE1 Human)
   

   Use

   Retroviral

   Tags

   acGFP

   Expression

   Mutation

   Serine 91, 262, 454, 724 Aspartate; Valine 53 Iso…

   Promoter

   CMV

   Available since

   Dec. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET3a.H2B-K120C

   Plasmid

   #224706

   Purpose

   Expresses Human H2B K120C mutant in bacterial cells for fluorescent labelling of histone octamers

   Depositor

   Chen Davidovich

   Article

   Uckelmann et al bioRxiv [Preprint]. 2024 Feb 12:2023.05.

   Insert

   H2B-K120C (H2B Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   K120C

   Promoter

   T7

   Available since

   Oct. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. p5E-EFS (EF1a core) (JDW 1319)

   Plasmid

   #224474

   Purpose

   Gateway compatible 5' entry clone with human alpha 2 globin pause site (to prevent transcription readthrough) and EF1a core promoter. Lacks downstream intron for a lower-activity, compact promoter.

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   EFS promoter
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pME-Actin-Vhh-sfGFP-P2A-1xHA-iRFP-caax (JDW 1216)

   Plasmid

   #224496

   Purpose

   Gateway compatible middle entry clone containing an Actin nanobody fused to sfGFP followed by P2A and iRFP-caax tag (GFP actin reporter and cell membrane iRFP reporter)

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   Actin-Vhh-sfGFP-P2A-HA-iRFP-caax
   

   Use

   Gateway cloning

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pME-LexA-mODC-LexOP-35SCaMV (JDW 1348)

   Plasmid

   #224505

   Purpose

   Gateway compatible middle entry clone containing a Destablized LexA and LexOperon with minimal CaMV 35S promoter as an all in one casette for RU486 driven gene expression

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   LexA-mODC-LexOP-35SCaMV
   

   Use

   Gateway cloning

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pME-LexA-mODC-LexOP-c-Fos (JDW 1359)

   Plasmid

   #224506

   Purpose

   Gateway compatible middle entry clone containing a Destablized LexA and LexOperon with 3' minimal c-Fos promoter for an all in one RU486 driven gene expression construct

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   LexA-oMDC-LexOP-c-Fos
   

   Use

   Gateway cloning

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pME-3xHA-BirA-T2A-mCherry-caax (JDW 889)

   Plasmid

   #224512

   Purpose

   A Gateway compatible middle entry clone containing Biotin Ligase followed by T2A and mCherry membrane reporter (biotinylation and flourescent cell membrane reporter)

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   3xHA-BirA-T2A-mCherry-Caax
   

   Use

   Gateway cloning

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pME-lox-3xNLS-mCherry-V5-SV40pA-3xstop-lox (JDW 1232)

   Plasmid

   #224522

   Purpose

   A Gateway compatible middle entry clone containing loxP flanked 3xNLS-mCherry-V5-3xSTOP cassette

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   lox-3xNLS-mCherry-V5-SV40pA-3xstop-lox
   

   Use

   Cre/Lox

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. p5E 5X C120 c-fos min pro (JDW 1241)

    Plasmid

    #224544

    Purpose

    A Gateway compatible 5' entry clone containing 5 TAEL transcription factor binding sites and a minimal c-Fos promoter

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    5x-C120-c-Fos promoter
    

    Use

    Gateway cloning

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. PGK_ACE2-ECD_Notch-Gal4VP64 (pZYW037)

    Plasmid

    #194226

    Purpose

    Lentiviral expression vector expressing ACE2-ECD SynNotch and mCherry transduction marker

    Depositor

    Hana El-Samad

    Article

    Weinberg et al bioRxiv 2021.04.20.440678

    Insert

    PGK-ACE2-SynNotch-T2A-mCherry (ACE2 Synthetic)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    PGK

    Available since

    Sept. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. SS-Myc-HexaPro-Foldon (MTK 3a) (pZYW077)

    Plasmid

    #194233

    Purpose

    Mammalian Toolkit part 3a encoding Jason McClellan lab's 6-proline Spike extracellular domain

    Depositor

    Hana El-Samad

    Article

    Weinberg et al bioRxiv 2021.04.20.440678

    Insert

    CD8a Signal Sequence-Myc Tag-SARS-CoV-2 Spike1-1258 (S Synthetic)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    None

    Available since

    Sept. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBK1297-AAV-EFSNC-dCjCas9-HP1b(79-173)

    Plasmid

    #223142

    Purpose

    Expression of truncated HP1b with dCjCas9 and empty gRNA scaffold

    Depositor

    Boris Kantor

    Article

    Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

    Insert

    HP1b (CBX1 Human)
    

    Use

    AAV and CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Encodes aa 79-173

    Promoter

    EF1a

    Available since

    Aug. 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pBK1295-AAV-EFSNC-dCjCas9-HP1a(72-177)

    Plasmid

    #223140

    Purpose

    Expression of truncated HP1a with dCjCas9 and empty gRNA scaffold

    Depositor

    Boris Kantor

    Article

    Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

    Insert

    HP1a (CBX5 Human)
    

    Use

    AAV and CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Encodes aa 72-177

    Promoter

    EF1a

    Available since

    Aug. 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pBK1305-AAV-EFSNC-dSaCas9-HP1a(72-177)

    Plasmid

    #223150

    Purpose

    Expression of truncated HP1a with dSaCas9 and empty gRNA scaffold

    Depositor

    Boris Kantor

    Article

    Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

    Insert

    HP1a (CBX5 Human)
    

    Use

    AAV and CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Encodes aa 72-177

    Promoter

    EF1a

    Available since

    Aug. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pBK1876-AAV-EFSNC-dSaCas9-KRAB-MECP2(APOE-gRNA2)

    Plasmid

    #223162

    Purpose

    Expression of KRAB and truncated MECP2 with dSaCas9 and gRNA2 targeting human/mouse APOE

    Depositor

    Boris Kantor

    Article

    Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

    Insert

    KRAB-MECP2 (MECP2 Human)
    

    Use

    AAV and CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Encodes aa 204-310 (MECP2)

    Promoter

    EF1a

    Available since

    Aug. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBK1298-AAV-EFSNC-dCjCas9-HP1bNH(111-173)

    Plasmid

    #223143

    Purpose

    Expression of truncated HP1b with dCjCas9 and empty gRNA scaffold

    Depositor

    Boris Kantor

    Article

    Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

    Insert

    HP1b (CBX1 Human)
    

    Use

    AAV and CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Encodes aa 111-173

    Promoter

    EF1a

    Available since

    Aug. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pBK1306-AAV-EFSNC-dSaCas9-HP1aNH(115-177)

    Plasmid

    #223151

    Purpose

    Expression of truncated HP1a with dSaCas9 and empty gRNA scaffold

    Depositor

    Boris Kantor

    Article

    Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

    Insert

    HP1a (CBX5 Human)
    

    Use

    AAV and CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Encodes aa 115-177

    Promoter

    EF1a

    Available since

    Aug. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pBK1296-AAV-EFSNC-dCjCas9-HP1aNH(115-177)

    Plasmid

    #223141

    Purpose

    Expression of truncated HP1a with dCjCas9 and empty gRNA scaffold

    Depositor

    Boris Kantor

    Article

    Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

    Insert

    HP1a (CBX5 Human)
    

    Use

    AAV and CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Encodes aa 115-177

    Promoter

    EF1a

    Available since

    Aug. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBK1308-AAV-EFSNC-dSaCas9-HP1bNH(111-173)

    Plasmid

    #223153

    Purpose

    Expression of truncated HP1b with dSaCas9 and empty gRNA scaffold

    Depositor

    Boris Kantor

    Article

    Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

    Insert

    HP1b (CBX1 Human)
    

    Use

    AAV and CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Encodes aa 111-173

    Promoter

    EF1a

    Available since

    Aug. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge