We narrowed to 9,383 results for: bli

1. pcDNA3.1-hSyncytin-1(C186A)

   Plasmid

   #182450

   Purpose

   hSyncytin-1, fusion deficit and dominant negative mutant (C186A)

   Depositor

   Masaharu Somiya

   Insert

   ERVW-1 (ERVW-1 Human)
   

   Expression

   Mammalian

   Mutation

   C186A

   Promoter

   CMV

   Available Since

   April 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAG Retro EGFP Puro

   Plasmid

   #234463

   Purpose

   Control retroviral vector expressing Green Fluorescent Protein. Puromycin selection.

   Depositor

   Melina Fan, Addgene Research Program

   Insert

   Green Fluorescent Protein
   

   Use

   Retroviral

   Available Since

   March 21, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pAG Retro EGFP Hygro

   Plasmid

   #234462

   Purpose

   Control retroviral vector expressing Green Fluorescent Protein. Hygromycin selection.

   Depositor

   Melina Fan, Addgene Research Program

   Insert

   Green Fluorescent Protein
   

   Use

   Retroviral

   Available Since

   March 21, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pCWU50

   Plasmid

   #232318

   Purpose

   A conditional plasmid system where RepA, required for pCWU6-based plasmid replication, is regulated by an inducible fdx promoter and theophylline-responsive riboswitch

   Depositor

   Chenggang Wu

   Insert

   Pfdx-E
   

   Expression

   Bacterial

   Promoter

   fdx

   Available Since

   Feb. 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. 1203AE-CMV-Cas7.1-7.2-H43A-mutant

   Plasmid

   #221449

   Purpose

   Effector expresses Cas7.1, Linker1, Cas11, Linker 2, and deactivated Cas7.2 domains from DiCas7-11, H to A mutation at amino acid 43 in Cas7.1

   Depositor

   Omar Akbari

   Insert

   Truncated DiCas7-11
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Feb. 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. 1203AF-CMV-Cas7.1-7.2-Y55A-mutant

   Plasmid

   #221450

   Purpose

   Effector expresses Cas7.1, Linker1, Cas11, Linker 2, and deactivated Cas7.2 domains from DiCas7-11, Y to A mutation at amino acid 55 in Cas7.1

   Depositor

   Omar Akbari

   Insert

   Truncated DiCas7-11
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Feb. 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. 1203AG-CMV-Cas7.1-7.2-N152A-mutant

   Plasmid

   #221451

   Purpose

   Effector expresses Cas7.1, Linker1, Cas11, Linker 2, and deactivated Cas7.2 domains from DiCas7-11, N to A mutation at amino acid 152 in Cas7.1

   Depositor

   Omar Akbari

   Insert

   Truncated DiCas7-11
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Feb. 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGEX-N-TEV-Twin-Strep

   Plasmid

   #222014

   Purpose

   Express C-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-tagged protein (ENLYFQGS-WSHPQFEK-(GGGS)2-GGSA-WSHPQFEK) in E.coli

   Depositor

   Chiaki Murakami

   Type

   Empty backbone

   Tags

   TEV cleavable site (ENLYFQG) and Twin-strep-tag

   Expression

   Bacterial

   Promoter

   lac promoter

   Available Since

   Jan. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSpec5'

   Plasmid

   #228549

   Purpose

   Template for PCR amplification for gRNA cloning plasmid. The PCR product recombines in vivo with a PCR product from pSpec3', to clone gRNA plasmid

   Depositor

   Jeferson Gross

   Insert

   Truncated 5' region of aadA
   

   Use

   CRISPR

   Available Since

   Dec. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSpec3'

    Plasmid

    #228548

    Purpose

    Template for PCR amplification for gRNA cloning plasmid. The PCR product recombines in vivo with a PCR product from pSpec5', to clone gRNA plasmid

    Depositor

    Jeferson Gross

    Insert

    Truncated 3' region of aadA
    

    Use

    CRISPR

    Available Since

    Dec. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pUSE-URA3-Sec7-mEYFP

    Plasmid

    #218968

    Purpose

    Gene replacement plasmid to label S. cerevisiae Sec7 with mEYFP

    Depositor

    Benjamin Glick

    Insert

    SEC7-mEYFP (SEC7 Budding Yeast)
    

    Use

    Gene replacement

    Tags

    mEYFP

    Available Since

    Oct. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pUSE-URA-Sec7-mGold

    Plasmid

    #218967

    Purpose

    Gene replacement plasmid to label S. cerevisiae Sec7 with mGold

    Depositor

    Benjamin Glick

    Insert

    SEC7-mGold (SEC7 Budding Yeast)
    

    Use

    Gene replacement vector for yeast

    Tags

    mGold

    Available Since

    Sept. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. YIplac211-Kar2-moxGFP2-HDEL

    Plasmid

    #218970

    Purpose

    Gene replacement plasmid to label S. cerevisiae Kar2 with moxGFP2

    Depositor

    Benjamin Glick

    Insert

    KAR2-moxGFP2-HDEL (KAR2 Budding Yeast)
    

    Use

    Gene replacement vector for yeast

    Tags

    moxGFP2

    Available Since

    Sept. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. MSCV-IRES-tagBFP

    Plasmid

    #219965

    Purpose

    express TagBFP in mammalian cells

    Depositor

    Mohamed Fareh

    Insert

    TagBFP
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    Sept. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. lentiGuideSLC35C1-neo

    Plasmid

    #220868

    Purpose

    encodes CRISPR sgRNA targeting human SLC3C1

    Depositor

    Jennifer Kohler

    Insert

    SLC35C1 (SLC35C1 Human)
    

    Use

    Lentiviral

    Available Since

    Sept. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. icLCT

    Plasmid

    #223179

    Purpose

    Native rat lactase gene (truncated) as an autologous marker (X-Gal, X-Fuc)

    Depositor

    Andreas Stuermer

    Insert

    cytosolic intestinal lactase protein fragment (Lct Rat)
    

    Expression

    Mammalian

    Available Since

    Aug. 26, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. pXR004_puroR

    Plasmid

    #219820

    Purpose

    hU6-driven pre-gRNA plasmid for CasRx applications with puromycin resistance. 5' processed DR followed by BbsI sites for guide cloning (Adapted from plasmid #10954)

    Depositor

    Philipp Maass

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. MUT_S7-H1

    Plasmid

    #218396

    Purpose

    3-exon minigene, with SRSF7 motif-rich middle exon, hnRNPA1 motif-rich region in second intron, and AG>GG mutation in final 3' splice site

    Depositor

    Oded Regev

    Insert

    Modified CHO DHFR minigene
    

    Expression

    Mammalian

    Promoter

    tetracycline-responsive promoter

    Available Since

    May 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. AAV-SYN-eGFP-oPRE

    Plasmid

    #218681

    Purpose

    AAV vector plasmid expressing enhanced green fluorescent protein (eGFP) under the human synapsin (SYN) promoter. It also contains an optimised and truncated version of WPRE as a regulatory element.

    Depositor

    Bart Nieuwenhuis

    Insert

    eGFP
    

    Use

    AAV

    Expression

    Mammalian

    Available Since

    May 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET-29b(+)_His-CBD-EmEndoF3

    Plasmid

    #218194

    Purpose

    Bacterial expression of a His-tagged Chitin Binding fusion of Elizabethkingia miricola EndoF3

    Depositor

    Florence Pojer

    Insert

    EndoF3
    

    Tags

    tufA-His-CBD

    Expression

    Bacterial

    Mutation

    codon optimized Ecoli, residues 41-329

    Promoter

    T7

    Available Since

    April 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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