We narrowed to 12,680 results for: sequence

1. pdCas9-humanized

   Plasmid

   #44246

   Purpose

   Expression of a catalytically inactive, human codon-optimized Cas9 under the control of Murine Stem Cell retroVirus LTR promoter for mammalian gene knockdown

   Depositor

   Stanley Qi

   Article

   Qi et al Cell. 2013 Feb 28;152(5):1173-83. doi: 1

   Insert

   dead Cas9 with 3X NLS
   

   Use

   CRISPR and Retroviral; Vector

   Tags

   3x NLS

   Expression

   Mammalian

   Mutation

   D10A H840A (catalytically inactive)

   Promoter

   MSCV LTR promoter

   Available since

   April 8, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. SERPINE2-bio-His

   Plasmid

   #53416

   Purpose

   Expresses full-length Glia-derived nexin precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

   Depositor

   Gavin Wright

   Article

   Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

   Insert

   SERPINE2 (SERPINE2 Human)
   

   Use

   Tags

   His tag, enzymatic biotinylation sequence, and ra…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAF0687 Pa01_attP-mCherry donor

   Plasmid

   #193466

   Purpose

   Promoterless mcherry donor for Pa01

   Depositor

   Patrick Hsu

   Article

   Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

   Insert

   Pa01_attP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. OpenCRISPR-1 sgRNA-16nt stem

   Plasmid

   #221566

   Purpose

   Express OpenCRISPR-1 sgRNA (16nt stem loop) in human cells using a U6 promoter. Plasmid also encodes a CMV-driven GFP reporter.

   Depositor

   Ali Madani

   Article

   Ruffolo et al bioRxiv 2024.04.22.590591

   Insert

   OpenCRISPR-1 sgRNA 16nt stem loop
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Aug. 28, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. OpenCRISPR-1 sgRNA-12nt stem

   Plasmid

   #221567

   Purpose

   Express OpenCRISPR-1 sgRNA (12nt stem loop) in human cells using a U6 promoter. Plasmid also encodes a CMV-driven GFP reporter.

   Depositor

   Ali Madani

   Article

   Ruffolo et al bioRxiv 2024.04.22.590591

   Insert

   OpenCRISPR-1 sgRNA 12nt stem loop
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Aug. 28, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pcDNA3-HA-CUL1 K720A

   Plasmid

   #19939

   Depositor

   Yue Xiong

   Article

   Furukawa et al Mol Cell Biol. 2000 Nov . 20(21):8185-97

   Insert

   cullin 1 K720A (CUL1 Human)
   

   Use

   Tags

   HA2

   Expression

   Mammalian

   Mutation

   CUL1 with a NEDD8 modification mutant

   Promoter

   Available since

   April 17, 2009

   Availability

   Academic Institutions and Nonprofits only

7. pAF0689 Ef1a-Pa01_attB-BFP acceptor

   Plasmid

   #193472

   Purpose

   Acceptor plasmid for 3 plasmid recombination assay

   Depositor

   Patrick Hsu

   Article

   Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

   Insert

   Pa01_attB
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Ef1a

   Available since

   Nov. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. EF1a_MYCN_P2A_Hygro_Barcode

   Plasmid

   #120463

   Purpose

   Barcoded lentiviral vector to express MYCN in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

   Depositor

   Prashant Mali

   Article

   Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

   Insert

   MYCN (MYCN Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   Feb. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3-HA-CUL1 Y42A/M43A

   Plasmid

   #19940

   Depositor

   Yue Xiong

   Article

   Furukawa et al Mol Cell Biol. 2000 Nov . 20(21):8185-97

   Insert

   cullin 1 Y42A/M43A (CUL1 Human)
   

   Use

   Tags

   HA2

   Expression

   Mammalian

   Mutation

   CUL1 with a SKP1 binding mutant

   Promoter

   Available since

   May 5, 2009

   Availability

   Academic Institutions and Nonprofits only

10. pcDNA3-HA-CUL1 delta 610-615

    Plasmid

    #19941

    Depositor

    Yue Xiong

    Article

    Furukawa et al Mol Cell Biol. 2000 Nov . 20(21):8185-97

    Insert

    cullin 1 delta 610-615 (CUL1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    CUL1 with a ROC1 binding mutant

    Promoter

    Available since

    April 28, 2009

    Availability

    Academic Institutions and Nonprofits only