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pFGC5941-PacI-ath-STTM828

Plasmid

#84157

Purpose

target miRNA828 for destruction in Arabidopsis thaliana

Depositor

Guiliang Tang

Article

Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

Insert

STTM828

Expression

Plant

Promoter

2x35S

Available Since

Feb. 14, 2017

Availability

Academic Institutions and Nonprofits only
pLentiGuide_GFP_sgEPAS1#1

Plasmid

#183119

Purpose

pLentiGuide modified to express GFP and an sgRNa targeting EPAS1

Depositor

William Kaelin

Article

Stransky et al Proc Natl Acad Sci U S A. 2022 Apr 5;119

Insert

sgEPAS1-1

Use

Lentiviral

Promoter

U6

Available Since

Aug. 24, 2022

Availability

Academic Institutions and Nonprofits only

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VYAIFIFQL_epitope

Plasmid

#165018

Purpose

Expression of CMV US20 (29-37) fused to IL-2 signal sequence

Depositor

Matthew Meyerson

Article

Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

Insert

CMV US20 (29-37)

Use

Lentiviral

Tags

human IL2 signal sequence at N-term

Expression

Mammalian

Promoter

CMV

Available Since

March 18, 2021

Availability

Academic Institutions and Nonprofits only

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pMB1-B-TelR

Plasmid

#204980

Purpose

Level 1 MoClo plasmid for A. baumannii with additional Tellurite resistance cassette for multi-drug resistance strains

Depositor

Veronica Godoy-Carter

Article

Brychcy et al ACS Synth Biol. 2023 Aug 16. doi: 10.102

Insert

mRFP1

Use

Synthetic Biology

Expression

Bacterial

Mutation

Added replications elements for Acinetobacter spe…

Available Since

Sept. 25, 2023

Availability

Academic Institutions and Nonprofits only

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p35S::mCh-HA-P2A-lifeact-mCh

Plasmid

#135234

Purpose

constitutive expression of 2 mCherry molecules: one tagged with HA and another that binds to actin (control plasmid)

Depositor

Armin Djamei

Article

Alcantara et al Plant Methods. 2020 Jan 21;16:4. doi: 10

Insert

mCh-HA-P2A-lifeact-mCh

Tags

HA, lifeact, and p2a

Expression

Plant

Promoter

35S

Available Since

Jan. 7, 2020

Availability

Academic Institutions and Nonprofits only

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AQTFAATHNPWASQA_epitope

Plasmid

#165019

Purpose

Expression of CMV UL86 (1222-1236) fused to CD74

Depositor

Matthew Meyerson

Article

Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

Insert

CMV UL86 (1222-1236)

Use

Lentiviral

Tags

CD74 at N-term; cathepsin S cleavage site (GRWHTV…

Expression

Mammalian

Promoter

CMV

Available Since

March 17, 2021

Availability

Academic Institutions and Nonprofits only

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pCETN1-IRFP670

Plasmid

#99879

Purpose

Expression of CETN1-iRFP670 in mammalian cells

Depositor

Marc Tramier

Article

Bertolin et al Nat Commun. 2016 Sep 14;7:12674. doi: 10

Insert

CETN1 (CETN1 Human)

Tags

iRFP670

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pFA0055

Plasmid

#131774

Purpose

Guide RNA (gCASS5a) and Cas9 expression plasmid for cleaving pFA6 series deletion cassettes, including KanMX, hphMX and natMX. Used to perform CRISPR-Swap of alleles.

Depositor

Frank Albert

Article

Lutz et al PLoS Genet. 2019 Nov 18;15(11):e1008375.

Insert

20mer CASS5a guide (gCASS5a) and 5' sgRNA

Expression

Bacterial and Yeast

Available Since

Oct. 7, 2019

Availability

Academic Institutions and Nonprofits only

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MAC_N_RAB5A

Plasmid

#111692

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Article

Liu et al Nat Commun. 2018 Mar 22;9(1):1188. doi:

Insert

RAB5A (RAB5A Human)

Expression

Mammalian

Available Since

June 14, 2018

Availability

Academic Institutions and Nonprofits only

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pFGC5941-PacI-ath-STTM843

Plasmid

#84169

Purpose

target miRNA843 for destruction in Arabidopsis thaliana

Depositor

Guiliang Tang

Article

Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

Insert

STTM843

Expression

Plant

Promoter

2x35S

Available Since

Feb. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pFGC5941-PacI-ath-STTM845

Plasmid

#84170

Purpose

target miRNA845 for destruction in Arabidopsis thaliana

Depositor

Guiliang Tang

Article

Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

Insert

STTM845

Expression

Plant

Promoter

2x35S

Available Since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

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sgRNA2_GAL4UAS-Luciferase reporter

Plasmid

#64158

Purpose

Photoactivatable transcription system. Lentiviral expression of sgRNA2 to target GAL4UAS-luciferase. Also contains a CMV-puro-t2A-mCherry expression cassette

Depositor

Moritoshi Sato

Article

Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

Insert

sgRNA2 for GAL4UAS-Luciferase reporter

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

April 15, 2015

Availability

Academic Institutions and Nonprofits only

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TAL2236

Plasmid

#36687

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

CHD4-TALEN_Left (Chd4 Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Promoter

CMV

Available Since

June 26, 2012

Availability

Academic Institutions and Nonprofits only
PTTG1IP_pCSdest

Plasmid

#53956

Depositor

Roger Reeves

Article

Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

Insert

PTTG1IP (PTTG1IP Human)

Expression

Mammalian

Promoter

SP6 and CMV

Available Since

Sept. 8, 2014

Availability

Industry, Academic Institutions, and Nonprofits
THI VP64 pGAP T-

Plasmid

#161487

Purpose

Non targeting control for CRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_VP64_SV40NLS_pGAP_THI11_T-_gRNA_MS2

Depositor

Brigitte Gasser, Diethard Mattanovich

Article

Baumschabl et al ACS Synth Biol. 2020 Dec 18;9(12):3202-3

Inserts

dCAS9
MS2-VP64
gRNA (Non targeting control NTC)

Expression

Yeast

Promoter

pGAP, pPOR1, and pTEF2

Available Since

March 15, 2021

Availability

Academic Institutions and Nonprofits only

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pLV-EF1a-GFP RanSen

Plasmid

#184705

Purpose

Lentiviral transfer plasmid for expression of GFP RanSen

Depositor

Brian Van Tine

Article

Rogers et al Cancer Metab. 2021 Jan 21;9(1):4. doi: 1

Insert

GFP RanSen

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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B108-Otx2-IRES-Cre

Plasmid

#73993

Purpose

B108 enhancer of Blimp1 gene drives Otx2 (isoform b) and Cre expression.

Depositor

Connie Cepko

Article

Wang et al Dev Cell. 2014 Sep 8;30(5):513-27. doi:

Insert

B108-Otx2 (isoform b)-IRES-Cre (Otx2 Mouse)

Expression

Mammalian

Available Since

July 27, 2017

Availability

Academic Institutions and Nonprofits only

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pFGC5941-PacI-ath-STTM173

Plasmid

#84101

Purpose

target miRNA173 for destruction in Arabidopsis thaliana

Depositor

Guiliang Tang

Article

Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

Insert

STTM173

Expression

Plant

Promoter

2x35S

Available Since

Nov. 29, 2016

Availability

Academic Institutions and Nonprofits only

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pMB1-D1-TelR

Plasmid

#204983

Purpose

Level 1 MoClo plasmid for A. baumannii with additional Tellurite resistance cassette for multi-drug resistance strains

Depositor

Veronica Godoy-Carter

Article

Brychcy et al ACS Synth Biol. 2023 Aug 16. doi: 10.102

Insert

mRFP1

Use

Synthetic Biology

Expression

Bacterial

Mutation

Added replications elements for Acinetobacter spe…

Available Since

Sept. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pBABE hygro rat IRS-3

Plasmid

#11450

Depositor

Ronald Kahn

Article

Tsuruzoe et al Mol Cell Biol. 2001 Jan . 21(1):26-38.

Insert

IRS-3 (Irs3 Rat)

Use

Retroviral

Expression

Mammalian

Available Since

March 3, 2006

Availability

Academic Institutions and Nonprofits only