We narrowed to 3,807 results for: golden gate

1. proUBI10::VIH2-FL::FLAG-P378V-G500V-G501A

   Plasmid

   #238338

   Purpose

   proUBI10::VIH2-FL::FLAG-P378V-G500V-G501A ubiquitous expression in Arabidopsis plants

   Depositor

   Michael Hothorn

   Article

   Raia et al Nat Commun. 2025 Feb 19;16(1):1753. doi:

   Insert

   VIP1 (VIP1 Mustard Weed)
   

   Tags

   3x Flag-tag

   Expression

   Plant

   Mutation

   AtVIH2-P378V-G500V-G501A

   Available Since

   June 12, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. proUBI10::VIH2-FL::FLAG-R722S-D724A-T726A

   Plasmid

   #238339

   Purpose

   proUBI10::VIH2-FL::FLAG-R722S-D724A-T726A ubiquitous expression in Arabidopsis plants

   Depositor

   Michael Hothorn

   Article

   Raia et al Nat Commun. 2025 Feb 19;16(1):1753. doi:

   Insert

   VIP1 (VIP1 Mustard Weed)
   

   Tags

   3x Flag-tag

   Expression

   Plant

   Mutation

   AtVIH2-R722S-D724A-T726A

   Available Since

   June 12, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. proUBI10::VIH2-FL::FLAG-K383A-K447A-K598A-R722A

   Plasmid

   #238340

   Purpose

   proUBI10::VIH2-FL::FLAG-K383A-K447A-K598A-R722A ubiquitous expression in Arabidopsis plants

   Depositor

   Michael Hothorn

   Article

   Raia et al Nat Commun. 2025 Feb 19;16(1):1753. doi:

   Insert

   VIP1 (VIP1 Mustard Weed)
   

   Tags

   3x Flag-tag

   Expression

   Plant

   Mutation

   AtVIH2-K383A-K447A-K598A-R722A

   Available Since

   June 12, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. proUBI10::VIH2-FL::FLAG-R372A-H373A-R376A-E882A

   Plasmid

   #238334

   Purpose

   proUBI10::VIH2-FL::FLAG-R372A-H373A-R376A-E882A ubiquitous expression in Arabidopsis plants

   Depositor

   Michael Hothorn

   Article

   Raia et al Nat Commun. 2025 Feb 19;16(1):1753. doi:

   Insert

   VIP1 (VIP1 Mustard Weed)
   

   Tags

   3x Flag-tag

   Expression

   Plant

   Mutation

   AtVIH2-R372A-H373A-R376A-E882A

   Available Since

   June 12, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. proUBI10::VIH2-FL::FLAG-K379A-K381A-K498A

   Plasmid

   #238341

   Purpose

   proUBI10::VIH2-FL::FLAG-K379A-K381A-K498A ubiquitous expression in Arabidopsis plants

   Depositor

   Michael Hothorn

   Article

   Raia et al Nat Commun. 2025 Feb 19;16(1):1753. doi:

   Insert

   VIP1 (VIP1 Mustard Weed)
   

   Tags

   3x Flag-tag

   Expression

   Plant

   Mutation

   AtVIH2-K379A-K381A-K498A

   Available Since

   June 12, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pLentiX1-[TRE3G-EGFP(miRE.FF4)-FMRP-TS.FF6x1-bGH]-EFS-rtTA-P2A-mRuby2-WPRE

   Plasmid

   #235307

   Purpose

   Lentiviral expression of ComMAND open-loop circuit regulating EGFP-FMRP (therapeutically relevant gene)

   Depositor

   Kate Galloway

   Article

   Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

   Insert

   EGFP-Fmr1 (Fmr1 Mouse)
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   TRE3G (3rd-generation Tet system)

   Available Since

   May 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pLentiX1-[TRE3G-FXN-P2A-mRuby2-bGH]-EFS-rtTA-P2A-mGL-WPRE

   Plasmid

   #235303

   Purpose

   Lentiviral expression of ComMAND base gene regulating FXN-P2A-mRuby2 (therapeutically relevant gene)

   Depositor

   Kate Galloway

   Article

   Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

   Insert

   FXN-P2A-mRuby2 (FXN Human)
   

   Use

   Lentiviral and Synthetic Biology

   Expression

   Mammalian

   Promoter

   TRE3G (3rd-generation Tet system)

   Available Since

   May 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pLentiX1-[TRE3G-FXN-P2A-mRuby2(miRE.FF4)-TS.FF6x1-bGH]-EFS-rtTA-P2A-mGL-WPRE

   Plasmid

   #235304

   Purpose

   Lentiviral expression of ComMAND open-loop circuit regulating FXN-P2A-mRuby2 (therapeutically relevant gene)

   Depositor

   Kate Galloway

   Article

   Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

   Insert

   FXN-P2A-mRuby2 (FXN Human)
   

   Use

   Lentiviral and Synthetic Biology

   Expression

   Mammalian

   Promoter

   TRE3G (3rd-generation Tet system)

   Available Since

   May 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pLentiX1-[TRE3G-EGFP(miRE.FF4)-FMRP-TS.FF4x1-bGH]-EFS-rtTA-P2A-mRuby2-WPRE

   Plasmid

   #235308

   Purpose

   Lentiviral expression of ComMAND closed-loop circuit regulating EGFP-FMRP (therapeutically relevant gene)

   Depositor

   Kate Galloway

   Article

   Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

   Insert

   EGFP-Fmr1 (Fmr1 Mouse)
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   TRE3G (3rd-generation Tet system)

   Available Since

   May 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLentiX1-[TRE3G-FXN-P2A-mRuby2(miRE.FF4)-TS.FF4x1-bGH]-EFS-rtTA-P2A-mGL-WPRE

    Plasmid

    #235305

    Purpose

    Lentiviral expression of ComMAND closed-loop circuit regulating FXN-P2A-mRuby2 (therapeutically relevant gene)

    Depositor

    Kate Galloway

    Article

    Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

    Insert

    FXN-P2A-mRuby2 (FXN Human)
    

    Use

    Lentiviral and Synthetic Biology

    Expression

    Mammalian

    Promoter

    TRE3G (3rd-generation Tet system)

    Available Since

    May 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pLentiX1-[TRE3G-EGFP-FMRP-bGH]-EFS-rtTA-P2A-mRuby2-WPRE

    Plasmid

    #235306

    Purpose

    Lentiviral expression of ComMAND base gene regulating EGFP-FMRP (therapeutically relevant gene)

    Depositor

    Kate Galloway

    Article

    Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

    Insert

    EGFP-Fmr1 (Fmr1 Mouse)
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    TRE3G (3rd-generation Tet system)

    Available Since

    May 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. lenti_U6_miR-Oct4e_TRE12_OCT4(co)-mNeonGreen_WPRE

    Plasmid

    #229544

    Purpose

    Lentiviral construct to express synthetic miRNA and OCT4-mNeonGreen fusion protein

    Depositor

    Domitilla Del Vecchio

    Article

    Ilia et al Sci Adv. 2023 Dec;9(48):eadg8495. doi: 1

    Insert

    Oct4(co)-mNeonGreen (POU5F1 Human)
    

    Use

    Lentiviral

    Tags

    Oct4-mNeonGreen

    Expression

    Mammalian

    Mutation

    codon-optimized OCT4

    Promoter

    TRE12

    Available Since

    Jan. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. PDG458 eSp(1.1) V3

    Plasmid

    #226964

    Purpose

    CBh-eSpCas9(1.1)-2A-GFP, and 2X hU6-sgRNA (Sp) with BbsI golden gate cloning backbone dual gRNAs. sgRNA uses 3TC scaffold for increased sgRNA expression.

    Depositor

    Paul Thomas

    Article

    Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Oct. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. HNRNPA1-miRFP670-TRF1

    Plasmid

    #227384

    Purpose

    Adhesion module, creates 'seed' at telomere

    Depositor

    Clifford Brangwynne

    Article

    Strom et al Cell. 2024 Aug 14:S0092-8674(24)00828-6.

    Insert

    Telomeric HNRNPA1 IDR (TERF1, HNRNPA1 Human)
    

    Use

    Lentiviral

    Available Since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET28:LanIV-Chondromyces

    Plasmid

    #225078

    Purpose

    Expresses precursor peptide and lanthipeptide synthetase from Chondromyces LanIV (FAST-RiPPs) in E. coli

    Depositor

    Wilfred van der Donk

    Article

    Ayikpoe et al Nat Commun. 2022 Oct 17;13(1):6135. doi:

    Insert

    LanA (LanIV-Chondromyces)
    

    Tags

    His6

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    T7

    Available Since

    Oct. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET28:LanII-56

    Plasmid

    #225073

    Purpose

    Expresses precursor peptide and lanthipeptide synthetase from LanII-56 (FAST-RiPPs) in E. coli

    Depositor

    Wilfred van der Donk

    Article

    Ayikpoe et al Nat Commun. 2022 Oct 17;13(1):6135. doi:

    Insert

    LanA (LanII-56)
    

    Tags

    His6

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    T7

    Available Since

    Oct. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET28:LanI-101

    Plasmid

    #225065

    Purpose

    Expresses precursor peptide, lanthipeptide cyclase, and lanthipeptide dehydratase from LanI-101 (FAST-RiPPs) in E. coli

    Depositor

    Wilfred van der Donk

    Article

    Ayikpoe et al Nat Commun. 2022 Oct 17;13(1):6135. doi:

    Insert

    LanA (LanI-101)
    

    Tags

    His6

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    T7

    Available Since

    Oct. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET28:LanII-2E

    Plasmid

    #225072

    Purpose

    Expresses precursor peptide and lanthipeptide synthetase from LanII-2E (FAST-RiPPs) in E. coli

    Depositor

    Wilfred van der Donk

    Article

    Ayikpoe et al Nat Commun. 2022 Oct 17;13(1):6135. doi:

    Insert

    LanA (LanII-2E)
    

    Tags

    His6

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    T7

    Available Since

    Oct. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET28:LanII-2D

    Plasmid

    #225071

    Purpose

    Expresses precursor peptide and lanthipeptide synthetase from LanII-2D (FAST-RiPPs) in E. coli

    Depositor

    Wilfred van der Donk

    Article

    Ayikpoe et al Nat Commun. 2022 Oct 17;13(1):6135. doi:

    Insert

    LanA (LanII-2D)
    

    Tags

    His6

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    T7

    Available Since

    Oct. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pMBS-859

    Plasmid

    #218009

    Purpose

    mCerulean fluorescent protein fused to Factor Xa Protease cleavage site and Strep-Tag II

    Depositor

    Michael Schroda

    Article

    Hieronimus et al Microb Cell. 2024 Apr 30;11:128-142. doi

    Insert

    mCerulean
    

    Use

    Synthetic Biology

    Tags

    Factor Xa protease cleavage site and Strep-tag II

    Available Since

    Aug. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge