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pAAV-nEF-Con/Foff 2.0-Arch3.3-EYFP

Plasmid

#137149

Purpose

Intersectional viral expression of Arch3.3-EYFP in cells expressing Cre AND NOT Flp

Depositor

Karl Deisseroth, INTRSECT 2.0 Project

Article

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Has Service

AAV8

Insert

Con/Foff 2.0-Arch3.3-EYFP

Use

AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

Mutation

n/a

Promoter

nEF

Available Since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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pCI-Puro_mouse_Adar2_Tv1

Plasmid

#158111

Purpose

expresses mADAR2 shortest transcript, puromycin selection

Depositor

Emanuela Felley-Bosco

Article

Abukar et al Cells. 2021 Dec 15;10(12). pii: cells101

Insert

adar2 (Adarb1 Mouse)

Expression

Mammalian

Available Since

Dec. 17, 2021

Availability

Academic Institutions and Nonprofits only

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Circular 200,100 with 8 bp bulge loops (GAPDH)

Plasmid

#170121

Purpose

AAV vector carrying a guide RNA targeting the human GAPDH mRNA

Depositor

Prashant Mali

Article

Katrekar et al Nat Biotechnol. 2022 Feb 10. pii: 10.103

Insert

Circular 200,100 guide RNA with 8bp bulge loops (GAPDH Human)

Use

AAV

Expression

Mammalian

Promoter

Human U6

Available Since

Feb. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-nEF-Con/Foff 2.0-bREACHes-EYFP

Plasmid

#137146

Purpose

Intersectional viral expression of bREACHes-EYFP in cells expressing Cre AND NOT Flp

Depositor

Karl Deisseroth, INTRSECT 2.0 Project

Article

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Insert

Con/Foff 2.0-bREACHes-EYFP

Use

AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

Mutation

n/a

Promoter

nEF

Available Since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-nEF-Coff/Fon-Arch3.3-p2a-EYFP

Plasmid

#137150

Purpose

Intersectional viral expression of Arch3.3-EYFP in cells expressing Flp AND NOT Cre

Depositor

Karl Deisseroth, INTRSECT 2.0 Project

Article

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Has Service

AAV8

Insert

Coff/Fon-Arch3.3-p2a-EYFP

Use

AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

Mutation

n/a

Promoter

nEF

Available Since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-nEF-Con/Foff 2.0-ChR2-mCherry

Plasmid

#137143

Purpose

Intersectional viral expression of ChR2-mCherry in cells expressing Cre AND NOT Flp

Depositor

Karl Deisseroth, INTRSECT 2.0 Project

Article

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Has Service

AAV8

Insert

Con/Foff 2.0-ChR2-mCherry

Use

AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

Mutation

H134R

Promoter

nEF

Available Since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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R791-X12-636: His6-MBP-tev-Hs.NF1 ABC (2-1540)

Plasmid

#159577

Purpose

Baculovirus protein expression of His6-MBP-tev-Hs.NF1 ABC (2-1540)

Depositor

Dominic Esposito

Article

Sherekar et al J Biol Chem. 2020 Jan 24;295(4):1105-111

Insert

His6-MBP-tev-Hs.NF1 ABC (2-1540) (NF1 Human)

Tags

His6-MBP-tev

Expression

Insect

Available Since

Sept. 29, 2020

Availability

Academic Institutions and Nonprofits only

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R791-X15-636: His6-MBP-tev-Hs.NF1 DEF (1541-2818)

Plasmid

#159578

Purpose

Baculovirus protein expression of His6-MBP-tev-Hs.NF1 DEF (1541-2818)

Depositor

Dominic Esposito

Article

Sherekar et al J Biol Chem. 2020 Jan 24;295(4):1105-111

Insert

His6-MBP-tev-Hs.NF1 DEF (1541-2818) (NF1 Human)

Tags

His6-MBP-tev

Expression

Insect

Available Since

Sept. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pBGE3.0

Plasmid

#165988

Purpose

Multi-host shuttle vector that can be transferred by conjugation and replicate in S. meliloti, E. coli, S. cerevisiae, and P. tricornutum. Contains S. meliloti pSymB origin and Nm/Kan resistance.

Depositor

Bogumil Karas

Article

Brumwell et al PLoS One. 2019 Jun 17;14(6):e0206781. do

Inserts

RK2/RP4 origin of transfer
repA1B1C1
nourseothricin N-acetyl transferase
neomycin resistance

Use

Synthetic Biology; Bacterial and yeast cloning; c…

Available Since

Dec. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pAGE2.0

Plasmid

#165984

Purpose

Multi-host shuttle vector that can be transferred by conjugation and replicate in S. meliloti, E. coli, S. cerevisiae, and P. tricornutum. Contains S. meliloti pSymA origin and Tet resistance.

Depositor

Bogumil Karas

Article

Brumwell et al PLoS One. 2019 Jun 17;14(6):e0206781. do

Inserts

RK2/RP4 origin of transfer
repA2B2C2
nourseothricin N-acetyl transferase
tetracycline resistance

Use

Synthetic Biology; Bacterial and yeast cloning; c…

Available Since

Oct. 27, 2022

Availability

Academic Institutions and Nonprofits only

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